Reviewed, UniProtKB/Swiss-Prot P09732 (POLG_STEVM)

Names and origin

Protein names	Recommended name:     Genome polyprotein Cleaved into the following 7 chains:     1- Recommended name:             Protein C         Alternative name(s):             Core protein             Capsid protein     2- Recommended name:             Small envelope protein M         Alternative name(s):             Matrix protein     3- Recommended name:             Envelope protein E     4- Recommended name:             Non-structural protein 1                 Short name=NS1     5- Recommended name:             Non-structural protein 2A                 Short name=NS2A     6- Recommended name:             Flavivirin protease NS2B regulatory subunit     7- Recommended name:             Flavivirin protease NS3 catalytic subunit               EC=3.4.21.91
Organism	St. louis encephalitis virus (strain MS1-7)
Taxonomic identifier	11081 [NCBI]
Taxonomic lineage	Viruses › ssRNA positive-strand viruses, no DNA stage › Flaviviridae › Flavivirus › Japanese encephalitis virus group
Virus host	Culex pipiens (House mosquito) [TaxID: 7175] Culex quinquefasciatus (Southern house mosquito) (Culex pungens) [TaxID: 7176] Culex tarsalis (Encephalitis mosquito) [TaxID: 7177] Columba livia (Domestic pigeon) [TaxID: 8932] Turdus migratorius (American robin) [TaxID: 9188] Agelaius tricolor (Tricolored blackbird) [TaxID: 9191] Homo sapiens (Human) [TaxID: 9606] Cyanocitta cristata (Blue jay) [TaxID: 28727] Carpodacus mexicanus (House finch) [TaxID: 30427] Culex nigripalpus [TaxID: 42429] Zenaida macroura (Mourning dove) [TaxID: 47245] Passer domesticus (House sparrow) [TaxID: 48849] Mimus polyglottos (Northern mockingbird) [TaxID: 60713] Euphagus cyanocephalus (Brewer's blackbird) [TaxID: 84817] Cardinalis cardinalis (Northern cardinal) [TaxID: 98964]

Protein attributes

Sequence length	1525 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The small proteins NS2A, NS4A and NS4B are hydrophobic, suggesting a possible membrane-related function. NS5 may play a role in the viral RNA replication. The NS2B/NS3 protease complex processes the viral polyprotein.
Catalytic activity	Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala. Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subunit structure	NS3 and NS2B form a heterodimer. NS3 is the catalytic subunit, whereas NS2B strongly stimulates the latter By similarity.
Subcellular location	Protein C: Virion Potential. Host membrane; Single-pass membrane protein Potential. Small envelope protein M: Virion Potential. Host membrane; Single-pass membrane protein Potential. Envelope protein E: Virion Potential. Host membrane; Multi-pass membrane protein Potential.
Post-translational modification	Specific enzymatic cleavages in vivo yield mature proteins By similarity.
Miscellaneous	The virion of this virus is a nucleocapsid covered by a lipoprotein envelope. The envelope contains two proteins: the protein M and glycoprotein E. The nucleocapsid is a complex of protein C and mRNA. In immature particles, there are 60 icosaedrally organized trimeric spikes on the surface. Each spike consists of three heterodimers of envelope protein M precursor (prM) and envelope protein E By similarity.
Sequence similarities	Contains 1 peptidase S7 domain.

Ontologies

Keywords
Cellular component	Capsid protein Core protein Envelope protein Membrane Virion
Domain	Transmembrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
PTM	Cleavage on pair of basic residues Disulfide bond Glycoprotein
Gene Ontology (GO)
Biological process	viral infectious cycle Inferred from electronic annotation. Source: InterPro
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW viral envelope Inferred from electronic annotation. Source: UniProtKB-KW viral nucleocapsid Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW double-stranded RNA binding Inferred from electronic annotation. Source: InterPro helicase activity Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro structural molecule activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed; by host
	Chain	2 – 121	120	Protein C		PRO_0000037735
	Propeptide	122 – 213	92			PRO_0000037736
	Chain	214 – 288	75	Small envelope protein M		PRO_0000037737
	Chain	289 – 789	501	Envelope protein E		PRO_0000037738
	Chain	790 – 1203	414	Non-structural protein 1		PRO_0000037739
	Chain	1204 – 1368	165	Non-structural protein 2A		PRO_0000037740
	Chain	1369 – 1499	131	Flavivirin protease NS2B regulatory subunit		PRO_0000037741
	Chain	1500 – ›1525	›26	Flavivirin protease NS3 catalytic subunit		PRO_0000037742
Regions
	Transmembrane	108 – 119	12	Potential
	Transmembrane	253 – 268	16	Potential
	Transmembrane	274 – 288	15	Potential
	Transmembrane	751 – 762	12	Potential
	Transmembrane	768 – 787	20	Potential
	Transmembrane	1173 – 1188	16	Potential
Amino acid modifications
	Glycosylation	136	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	269	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	442	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	919	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	964	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	996	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	1189	1	N-linked (GlcNAc...); by host Potential
	Disulfide bond	291 ↔ 318		By similarity
	Disulfide bond	348 ↔ 404		By similarity
	Disulfide bond	362 ↔ 393		By similarity
	Disulfide bond	380 ↔ 409		By similarity
	Disulfide bond	478 ↔ 576		By similarity
	Disulfide bond	593 ↔ 624		By similarity
Experimental info
	Non-terminal residue	1525	1

Sequences

	Sequence		Length	Mass (Da)
	P09732-1 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: E1A373F1E511159F	FASTA	1,525	167,892
	10 20 30 40 50 60 MSKKPGKPGR NRVVNMLKRG VSRVNPLTGL KRILGSLLDG RGPVRFMLAI LTFFRFTALQ 70 80 90 100 110 120 PTEALKRRWR AVDKRTALKH LNGFKRDLGS MLDTINRRPS KKRGGTRSLL GLAALIGLAS 130 140 150 160 170 180 SLQLSTYQGK VLMSINKTDA QSAINIPSAN GANTCIVRAL DVGIMCKDDI TYLCPVLSAG 190 200 210 220 230 240 NDPEDIDCWC DVEEVWVHYG RCTRMGHSRR SRRSISVQHH GDSTLATKNT PWLDTVKTTK 250 260 270 280 290 300 YLTKVENWFC RNPGYALVAL AIGWMLGSNN TQRVVFVIML MLIAPAYSFN CLGTSNRDFV 310 320 330 340 350 360 EGASGATWID LVLEGGSCVT VMAPEKPTLD FKVMKMEATE LATVRKYCYE ATLDTLSTVA 370 380 390 400 410 420 RCPTTGEAHN TKRSDPTFVC KRDVVDRGWG NGCGLFGKGS IDTCAKFTCK NKATGKTILR 430 440 450 460 470 480 ENIKYEVAIF VHGSTDSTSH GNYSEQIGKN QAARFTISPQ APSFTANMGE YGTVTIDCEA 490 500 510 520 530 540 RSGINTEDYY VFTVKEKSWL VNRDWFHDLN LPWTSPATTD WRNRETLVEF EEPHATKQTV 550 560 570 580 590 600 VALGSQEGAL HTALAGAIPA TVSSSTLTLQ SGHLKCRAKL DKVKIKGTTY GMCDSAFTFS 610 620 630 640 650 660 KNPTDTGHGT VIVELQYTGS NGPCRVPISV TANLMDLTPV GRLVTVNPFI STGGANNKVM 670 680 690 700 710 720 IEVEPPFGDS YIVVGRGTTQ INYHWHKEGS SIGKALATTW KGAQRLAVLG DTAWDFGSIG 730 740 750 760 770 780 GVFNSIGKAV HQVFGGAFRT LFGGMSWITQ GLLGALLLWM GLQARDRSIS LTLLAVGGIL 790 800 810 820 830 840 IFLATSVQAD SGCAISLQRR ELKCGGGIFV YNDVEKWKSD YKYFPLTPTG LAHVIQEAHA 850 860 870 880 890 900 NGYCGIRSTS RLEHLMWENI QRELNAIFED NEIDLSVVVQ EDPKYYKRAP RRLKKLEDEL 910 920 930 940 950 960 DYGWKKWGKT LFVEPRLGNN TFVVDGPETK ECPTANRAWN SFKVEDFGFG MVFTQLWLTI 970 980 990 1000 1010 1020 REENTTECDS AIIGTAIKGD RAVHSDLSYW IESKKNETWQ LERAVMGEVK SCTWPETHTL 1030 1040 1050 1060 1070 1080 WGDGVVESEM IIPVTLGGPK SHHNKRNGYH TQTKGPWSEG EITLDFDYCP GTTVTVTEHC 1090 1100 1110 1120 1130 1140 GNRGASLRTT TASGKLVTDW CCRSCSLPPL RYTTKDGCWY GMEIRPVKEE EAKLVKSRVT 1150 1160 1170 1180 1190 1200 AGVAGGMEPF QLGLLVAFIA TQEVLKRRWT GKLTLTSLAV CLALLIFGNL TYMDLVRYLV 1210 1220 1230 1240 1250 1260 LVGTAFAEMN TGGDVIHLAL VAVFKVQPAF LAGLFLRMQW SNQENILMVI GAAFLQMAAN 1270 1280 1290 1300 1310 1320 DLKLEVLPIL NAMSIAWMLI RAMKEGKVAM YALPILCALT PGMRMAGLDV IRCLLLIIGI 1330 1340 1350 1360 1370 1380 VTLLNERRES VAKKKGGYLL AAALCQAGVC SPLIMMGGLI LAHPNGKRSW PASEVLTGVG 1390 1400 1410 1420 1430 1440 LMCALAGGLL EFEETSMVVP FAIAGLMYIT YTVSGKAAEM WIEKAADITW EQNAEITGTS 1450 1460 1470 1480 1490 1500 PRLDVDLDSH GNFKLLNDPG APVHLFALRF ILLGLSARFH WFIPFGVLGF WLLGKHSKRG 1510 1520 GALWDVPSPK VYPKCETKPG IYRIM

References

[1]

"Partial nucleotide sequence of St. Louis encephalitis virus RNA: structural proteins, NS1, NS2A, and NS2B." Trent D.W., Kinney R.M., Johnson B.J.B., Vorndam A.V., Grant J.A., Deubel V., Rice C.M., Hahn C. Virology 156:293-304(1987) [PubMed: 3027980] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	M16614 Genomic RNA. Translation: AAA47786.1.
3D structure databases
SMR	P09732. Positions 289-691.
ModBase	Search...
Family and domain databases
InterPro	IPR000069. Env_glycoprot_M_flavivir. IPR013756. Flav_glyE_cen_2. IPR011999. Flav_glyE_cen_dm. IPR013754. Flav_glyE_dim. IPR001122. Flavi_capsidC. IPR001157. Flavi_NS1. IPR000752. Flavi_NS2A. IPR000487. Flavi_NS2B. IPR002535. Flavi_propep. IPR000336. Flv_glyE_Ig-like. [Graphical view]
Gene3D	G3DSA:3.30.67.10. Flav_glyE_cen_2. 1 hit. G3DSA:2.60.98.10. Flav_glyE_dim. 1 hit. G3DSA:2.60.40.350. Flv_glyE_Ig-like. 1 hit.
Pfam	PF01003. Flavi_capsid. 1 hit. PF02832. Flavi_glycop_C. 1 hit. PF00869. Flavi_glycoprot. 1 hit. PF01004. Flavi_M. 1 hit. PF00948. Flavi_NS1. 1 hit. PF01005. Flavi_NS2A. 1 hit. PF01002. Flavi_NS2B. 1 hit. PF01570. Flavi_propep. 1 hit. [Graphical view]
ProDom	PD001496. Flavi_NS1. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

POLG_STEVM

Accession

Primary (citable) accession number: P09732
Secondary accession number(s): Q88781 , Q88782, Q88783, Q88784, Q88785, Q88786, Q88787, Q88788

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	June 16, 2009
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)