P09693 (CD3G_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
T-cell surface glycoprotein CD3 gamma chain

Alternative name(s):
T-cell receptor T3 gamma chain
CD_antigen=CD3g

Gene names

Name:	CD3G
Synonyms:	T3G

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	182 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The CD3 complex mediates signal transduction.
Subunit structure	The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. Ref.8
Subcellular location	Membrane; Single-pass type I membrane protein.
Domain	A di-leucine motif and a tyrosine-based motif are individually sufficient to induce both endocytosis and delivery to lysosomes.
Sequence similarities	Contains 1 Ig-like (immunoglobulin-like) domain. Contains 1 ITAM domain.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	Immunoglobulin domain Signal Transmembrane Transmembrane helix
Molecular function	Receptor
PTM	Disulfide bond Glycoprotein Phosphoprotein
Technical term	3D-structure Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	Fc-gamma receptor signaling pathway involved in phagocytosis Traceable author statement. Source: Reactome T cell activation Non-traceable author statement PubMed 12407027. Source: UniProtKB T cell costimulation Traceable author statement. Source: Reactome T cell receptor signaling pathway Traceable author statement. Source: Reactome establishment or maintenance of cell polarity Inferred from mutant phenotype PubMed 12407027. Source: UniProtKB innate immune response Traceable author statement. Source: Reactome protein complex assembly Non-traceable author statement PubMed 12794121. Source: UniProtKB protein transport Inferred from mutant phenotype PubMed 12794121. Source: UniProtKB regulation of apoptotic process Inferred from mutant phenotype PubMed 12407027. Source: UniProtKB
Cellular_component	T cell receptor complex Non-traceable author statement PubMed 9485181. Source: UniProtKB alpha-beta T cell receptor complex Inferred from electronic annotation. Source: Compara integral to plasma membrane Non-traceable author statement PubMed 9485181. Source: UniProtKB
Molecular_function	T cell receptor binding Non-traceable author statement PubMed 11186279. Source: UniProtKB receptor signaling complex scaffold activity Non-traceable author statement PubMed 12794121. Source: UniProtKB transmembrane signaling receptor activity Inferred from mutant phenotype PubMed 12794121. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 22

22

Chain

23 – 182

160

T-cell surface glycoprotein CD3 gamma chain

PRO_0000014615

Regions

Topological domain

23 – 116

94

Extracellular Potential

Transmembrane

117 – 137

21

Helical; Potential

Topological domain

138 – 182

45

Cytoplasmic Potential

Domain

37 – 94

58

Ig-like

Domain

149 – 177

29

ITAM

Motif

153 – 154

2

Di-leucine motif

Amino acid modifications

Modified residue

145

1

Phosphoserine Ref.4 Ref.5

Modified residue

148

1

Phosphoserine Ref.4 Ref.5

Glycosylation

52

1

N-linked (GlcNAc...) Potential

Glycosylation

92

1

N-linked (GlcNAc...) Potential

Disulfide bond

46 ↔ 87

Ref.8

Natural variations

Natural variant

131

1

V → F.
Corresponds to variant rs3753058 [ dbSNP | Ensembl ].

VAR_049854

Experimental info

Mutagenesis

153

1

L → A: Abolishes lysosomal targeting. Ref.6

Mutagenesis

153

1

L → I: Diminished but persistent lysosomal targeting. Ref.6

Mutagenesis

154

1

L → A: Diminished but persistent lysosomal targeting. Ref.6

Mutagenesis

154

1

L → I: No effect. Ref.6

Mutagenesis

160

1

Y → A: Abolishes lysosomal targeting. Ref.6

Mutagenesis

163

1

L → A: Abolishes lysosomal targeting. Ref.6

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

182

Helix Strand Turn

Details...

Beta strand

31 – 33

3

Beta strand

43 – 46

4

Beta strand

50 – 57

8

Beta strand

65 – 68

4

Helix

77 – 79

3

Beta strand

85 – 88

4

Beta strand

97 – 102

6

Sequences

	Sequence		Length	Mass (Da)
	P09693 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: EE65C0186FB9872B	FASTA	182	20,469
	10 20 30 40 50 60 MEQGKGLAVL ILAIILLQGT LAQSIKGNHL VKVYDYQEDG SVLLTCDAEA KNITWFKDGK 70 80 90 100 110 120 MIGFLTEDKK KWNLGSNAKD PRGMYQCKGS QNKSKPLQVY YRMCQNCIEL NAATISGFLF 130 140 150 160 170 180 AEIVSIFVLA VGVYFIAGQD GVRQSRASDK QTLLPNDQLY QPLKDREDDQ YSHLQGNQLR RN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Primary structure of the T3 gamma subunit of the T3/T cell antigen receptor complex deduced from cDNA sequences: evolution of the T3 gamma and delta subunits." Krissansen G.W., Owen M.J., Verbi W., Crumpton M.J. EMBO J. 5:1799-1808(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Physical linkage of three CD3 genes on human chromosome 11." Tunnacliffe A., Buluwela L., Rabbitts T.H. EMBO J. 6:2953-2957(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"Dephosphorylation of the human T lymphocyte CD3 antigen." Alexander D., Goris J., Marais R., Rothbard J., Merlevede W., Crumpton M.J. Eur. J. Biochem. 181:55-65(1989) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 139-182, PHOSPHORYLATION AT SER-145 AND SER-148.
[5]	"The human T3 gamma chain is phosphorylated at serine 126 in response to T lymphocyte activation." Davies A.A., Cantrell D.A., Hexham J.M., Parker P.J., Rothbard J., Crumpton M.J. J. Biol. Chem. 262:10918-10921(1987) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION AT SER-145 AND SER-148.
[6]	"A novel di-leucine motif and a tyrosine-based motif independently mediate lysosomal targeting and endocytosis of CD3 chains." Letourneur F., Klausner R.D. Cell 69:1143-1157(1992) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS OF LEU-153; LEU-154; TYR-160 AND LEU-163.
[7]	"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions." Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K. Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Tissue: Leukemic T-cell.
[8]	"Crystal structure of the human T cell receptor CD3 epsilon gamma heterodimer complexed to the therapeutic mAb OKT3." Kjer-Nielsen L., Dunstone M.A., Kostenko L., Ely L.K., Beddoe T., Mifsud N.A., Purcell A.W., Brooks A.G., McCluskey J., Rossjohn J. Proc. Natl. Acad. Sci. U.S.A. 101:7675-7680(2004) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 23-103 IN COMPLEX WITH CD3E AND ANTIBODY, SUBUNIT, DISULFIDE BOND.
+	Additional computationally mapped references.

Web resources

CD3Gbase

CD3G mutation db

Wikipedia

CD3 receptor entry

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X04145 mRNA. Translation: CAA27764.1.
X06026 , X06027, X06028, X06029, X06030, X06031 Genomic DNA. Translation: CAA29428.1.
BC113830 mRNA. Translation: AAI13831.1.

IPI

IPI00016020.

PIR

A25468.

RefSeq

NP_000064.1. NM_000073.2.

UniGene

Hs.2259.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1SY6	X-ray	2.10	A	23-103	[»]

DisProt

DP00508.

ProteinModelPortal

P09693.

ModBase

Search...

Protein-protein interaction databases

IntAct

P09693. 1 interaction.

MINT

MINT-4656150.

STRING

9606.ENSP00000292144.

PTM databases

PhosphoSite

P09693.

Polymorphism databases

DMDM

115993.

Proteomic databases

PaxDb

P09693.

PeptideAtlas

P09693.

PRIDE

P09693.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENST00000532917; ENSP00000431445; ENSG00000160654.

GeneID

917.

KEGG

hsa:917.

UCSC

uc001psu.2. human.

Organism-specific databases

CTD

917.

GeneCards

GC11P118248.

HGNC

HGNC:1675. CD3G.

HPA

CAB017520.

MIM

186740. gene+phenotype.

neXtProt

NX_P09693.

Orphanet

169082. Combined immunodeficiency due to CD3gamma deficiency.

PharmGKB

PA26217.

GenAtlas

Search...

Phylogenomic databases

eggNOG

NOG43749.

HOGENOM

HOG000015287.

HOVERGEN

HBG005278.

InParanoid

P09693.

KO

K06452.

OMA

QNCIELS.

OrthoDB

EOG405S26.

PhylomeDB

P09693.

Enzyme and pathway databases

Pathway_Interaction_DB

cd8tcrdownstreampathway. Downstream signaling in naive CD8+ T cells.
il12_stat4pathway. IL12 signaling mediated by STAT4.
il12_2pathway. IL12-mediated signaling events.
tcrpathway. TCR signaling in naive CD4+ T cells.
cd8tcrpathway. TCR signaling in naive CD8+ T cells.

Reactome

REACT_6900. Immune System.

Gene expression databases

ArrayExpress

P09693.

Bgee

P09693.

CleanEx

HS_CD3G.

Genevestigator

P09693.

GermOnline

ENSG00000160654. Homo sapiens.

Family and domain databases

Gene3D

2.60.40.10. 1 hit.

InterPro

IPR015484. CD3_gsu/dsu.
IPR013783. Ig-like_fold.
IPR003598. Ig_sub2.
IPR003110. Phos_immunorcpt_sig_ITAM.
[Graphical view]

PANTHER

PTHR10570. PTHR10570. 1 hit.

Pfam

PF02189. ITAM. 1 hit.
[Graphical view]

SMART

SM00408. IGc2. 1 hit.
SM00077. ITAM. 1 hit.
[Graphical view]

PROSITE

PS50835. IG_LIKE. False negative.
PS51055. ITAM_1. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

ChiTaRS

CD3G. human.

EvolutionaryTrace

P09693.

GenomeRNAi

917.

NextBio

3794.

SOURCE

Search...

Entry information

Entry name

CD3G_HUMAN

Accession

Primary (citable) accession number: P09693
Secondary accession number(s): Q2HIZ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	May 29, 2013
This is version 131 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.