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P09693

- CD3G_HUMAN

UniProt

P09693 - CD3G_HUMAN

Protein

T-cell surface glycoprotein CD3 gamma chain

Gene

CD3G

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 145 (01 Oct 2014)
      Sequence version 1 (01 Jul 1989)
      Previous versions | rss
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    Functioni

    The CD3 complex mediates signal transduction.

    GO - Molecular functioni

    1. protein heterodimerization activity Source: UniProtKB
    2. receptor signaling complex scaffold activity Source: UniProtKB
    3. T cell receptor binding Source: UniProtKB
    4. transmembrane signaling receptor activity Source: UniProtKB

    GO - Biological processi

    1. cell surface receptor signaling pathway Source: UniProtKB
    2. establishment or maintenance of cell polarity Source: UniProtKB
    3. Fc-gamma receptor signaling pathway involved in phagocytosis Source: Reactome
    4. innate immune response Source: Reactome
    5. protein complex assembly Source: UniProtKB
    6. protein transport Source: UniProtKB
    7. regulation of immune response Source: Reactome
    8. regulation of lymphocyte apoptotic process Source: UniProtKB
    9. T cell activation Source: UniProtKB
    10. T cell costimulation Source: Reactome
    11. T cell receptor signaling pathway Source: Reactome

    Keywords - Molecular functioni

    Receptor

    Enzyme and pathway databases

    ReactomeiREACT_11152. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
    REACT_12555. Downstream TCR signaling.
    REACT_12582. Phosphorylation of CD3 and TCR zeta chains.
    REACT_12596. Translocation of ZAP-70 to Immunological synapse.
    REACT_12623. Generation of second messenger molecules.
    REACT_160086. Regulation of actin dynamics for phagocytic cup formation.
    REACT_160158. Role of phospholipids in phagocytosis.
    REACT_160274. FCGR activation.
    REACT_19324. PD-1 signaling.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    T-cell surface glycoprotein CD3 gamma chain
    Alternative name(s):
    T-cell receptor T3 gamma chain
    CD_antigen: CD3g
    Gene namesi
    Name:CD3G
    Synonyms:T3G
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 11

    Organism-specific databases

    HGNCiHGNC:1675. CD3G.

    Subcellular locationi

    GO - Cellular componenti

    1. alpha-beta T cell receptor complex Source: Ensembl
    2. integral component of plasma membrane Source: UniProtKB
    3. plasma membrane Source: Reactome
    4. T cell receptor complex Source: UniProtKB

    Keywords - Cellular componenti

    Membrane

    Pathology & Biotechi

    Involvement in diseasei

    Immunodeficiency 17 (IMD17) [MIM:615607]: An autosomal recessive primary immunodeficiency characterized by highly variable clinical severity. Some patients have onset of severe recurrent infections in early infancy that may be lethal, whereas others may be only mildly affected or essentially asymptomatic into young adulthood. More severely affected patients may have evidence of autoimmune disease or enteropathy. The immunologic pattern is similar among patients, showing partial T-cell lymphopenia, decreased amounts of the CD3 complex, and impaired proliferative responses to T-cell receptor dependent stimuli. The phenotype in some patients is reminiscent of severe combined immunodeficiency.2 Publications
    Note: The disease is caused by mutations affecting the gene represented in this entry.

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi153 – 1531L → A: Abolishes lysosomal targeting. 1 Publication
    Mutagenesisi153 – 1531L → I: Diminished but persistent lysosomal targeting. 1 Publication
    Mutagenesisi154 – 1541L → A: Diminished but persistent lysosomal targeting. 1 Publication
    Mutagenesisi154 – 1541L → I: No effect. 1 Publication
    Mutagenesisi160 – 1601Y → A: Abolishes lysosomal targeting. 1 Publication
    Mutagenesisi163 – 1631L → A: Abolishes lysosomal targeting. 1 Publication

    Organism-specific databases

    MIMi615607. phenotype.
    Orphaneti169082. Combined immunodeficiency due to CD3gamma deficiency.
    PharmGKBiPA26217.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2222Add
    BLAST
    Chaini23 – 182160T-cell surface glycoprotein CD3 gamma chainPRO_0000014615Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi46 ↔ 871 Publication
    Glycosylationi52 – 521N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi92 – 921N-linked (GlcNAc...)Sequence Analysis
    Modified residuei145 – 1451Phosphoserine2 Publications
    Modified residuei148 – 1481Phosphoserine2 Publications

    Keywords - PTMi

    Disulfide bond, Glycoprotein, Phosphoprotein

    Proteomic databases

    MaxQBiP09693.
    PaxDbiP09693.
    PeptideAtlasiP09693.
    PRIDEiP09693.

    PTM databases

    PhosphoSiteiP09693.

    Expressioni

    Gene expression databases

    ArrayExpressiP09693.
    BgeeiP09693.
    CleanExiHS_CD3G.
    GenevestigatoriP09693.

    Organism-specific databases

    HPAiCAB017520.
    HPA038494.

    Interactioni

    Subunit structurei

    The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta.1 Publication

    Protein-protein interaction databases

    BioGridi107355. 4 interactions.
    IntActiP09693. 1 interaction.
    MINTiMINT-4656150.
    STRINGi9606.ENSP00000292144.

    Structurei

    Secondary structure

    1
    182
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi31 – 333
    Beta strandi43 – 464
    Beta strandi50 – 578
    Beta strandi65 – 684
    Helixi77 – 793
    Beta strandi85 – 884
    Beta strandi97 – 1026

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1SY6X-ray2.10A23-103[»]
    DisProtiDP00508.
    ProteinModelPortaliP09693.
    SMRiP09693. Positions 23-103.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP09693.

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini23 – 11694ExtracellularSequence AnalysisAdd
    BLAST
    Topological domaini138 – 18245CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei117 – 13721HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini37 – 9458Ig-likeAdd
    BLAST
    Domaini149 – 17729ITAMPROSITE-ProRule annotationAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi153 – 1542Di-leucine motif

    Domaini

    A di-leucine motif and a tyrosine-based motif are individually sufficient to induce both endocytosis and delivery to lysosomes.

    Sequence similaritiesi

    Contains 1 ITAM domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Immunoglobulin domain, Signal, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG43749.
    HOGENOMiHOG000015287.
    HOVERGENiHBG005278.
    InParanoidiP09693.
    KOiK06452.
    OMAiDRENDQY.
    OrthoDBiEOG7S4X7Q.
    PhylomeDBiP09693.
    TreeFamiTF335892.

    Family and domain databases

    Gene3Di2.60.40.10. 1 hit.
    InterProiIPR015484. CD3_esu/gsu/dsu.
    IPR013783. Ig-like_fold.
    IPR003598. Ig_sub2.
    IPR003110. Phos_immunorcpt_sig_ITAM.
    [Graphical view]
    PANTHERiPTHR10570. PTHR10570. 1 hit.
    PfamiPF02189. ITAM. 1 hit.
    [Graphical view]
    SMARTiSM00408. IGc2. 1 hit.
    SM00077. ITAM. 1 hit.
    [Graphical view]
    PROSITEiPS51055. ITAM_1. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P09693-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEQGKGLAVL ILAIILLQGT LAQSIKGNHL VKVYDYQEDG SVLLTCDAEA    50
    KNITWFKDGK MIGFLTEDKK KWNLGSNAKD PRGMYQCKGS QNKSKPLQVY 100
    YRMCQNCIEL NAATISGFLF AEIVSIFVLA VGVYFIAGQD GVRQSRASDK 150
    QTLLPNDQLY QPLKDREDDQ YSHLQGNQLR RN 182
    Length:182
    Mass (Da):20,469
    Last modified:July 1, 1989 - v1
    Checksum:iEE65C0186FB9872B
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti131 – 1311V → F.
    Corresponds to variant rs3753058 [ dbSNP | Ensembl ].
    VAR_049854

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X04145 mRNA. Translation: CAA27764.1.
    X06026
    , X06027, X06028, X06029, X06030, X06031 Genomic DNA. Translation: CAA29428.1.
    BC113830 mRNA. Translation: AAI13831.1.
    CCDSiCCDS8395.1.
    PIRiA25468.
    RefSeqiNP_000064.1. NM_000073.2.
    XP_006719004.1. XM_006718941.1.
    UniGeneiHs.2259.

    Genome annotation databases

    EnsembliENST00000532917; ENSP00000431445; ENSG00000160654.
    GeneIDi917.
    KEGGihsa:917.
    UCSCiuc001psu.2. human.

    Polymorphism databases

    DMDMi115993.

    Keywords - Coding sequence diversityi

    Polymorphism

    Cross-referencesi

    Web resourcesi

    CD3Gbase

    CD3G mutation db

    Wikipedia

    CD3 receptor entry

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X04145 mRNA. Translation: CAA27764.1 .
    X06026
    , X06027 , X06028 , X06029 , X06030 , X06031 Genomic DNA. Translation: CAA29428.1 .
    BC113830 mRNA. Translation: AAI13831.1 .
    CCDSi CCDS8395.1.
    PIRi A25468.
    RefSeqi NP_000064.1. NM_000073.2.
    XP_006719004.1. XM_006718941.1.
    UniGenei Hs.2259.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1SY6 X-ray 2.10 A 23-103 [» ]
    DisProti DP00508.
    ProteinModelPortali P09693.
    SMRi P09693. Positions 23-103.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 107355. 4 interactions.
    IntActi P09693. 1 interaction.
    MINTi MINT-4656150.
    STRINGi 9606.ENSP00000292144.

    Chemistry

    ChEMBLi CHEMBL2364168.

    PTM databases

    PhosphoSitei P09693.

    Polymorphism databases

    DMDMi 115993.

    Proteomic databases

    MaxQBi P09693.
    PaxDbi P09693.
    PeptideAtlasi P09693.
    PRIDEi P09693.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000532917 ; ENSP00000431445 ; ENSG00000160654 .
    GeneIDi 917.
    KEGGi hsa:917.
    UCSCi uc001psu.2. human.

    Organism-specific databases

    CTDi 917.
    GeneCardsi GC11P118248.
    HGNCi HGNC:1675. CD3G.
    HPAi CAB017520.
    HPA038494.
    MIMi 186740. gene.
    615607. phenotype.
    neXtProti NX_P09693.
    Orphaneti 169082. Combined immunodeficiency due to CD3gamma deficiency.
    PharmGKBi PA26217.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG43749.
    HOGENOMi HOG000015287.
    HOVERGENi HBG005278.
    InParanoidi P09693.
    KOi K06452.
    OMAi DRENDQY.
    OrthoDBi EOG7S4X7Q.
    PhylomeDBi P09693.
    TreeFami TF335892.

    Enzyme and pathway databases

    Reactomei REACT_11152. Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell.
    REACT_12555. Downstream TCR signaling.
    REACT_12582. Phosphorylation of CD3 and TCR zeta chains.
    REACT_12596. Translocation of ZAP-70 to Immunological synapse.
    REACT_12623. Generation of second messenger molecules.
    REACT_160086. Regulation of actin dynamics for phagocytic cup formation.
    REACT_160158. Role of phospholipids in phagocytosis.
    REACT_160274. FCGR activation.
    REACT_19324. PD-1 signaling.

    Miscellaneous databases

    ChiTaRSi CD3G. human.
    EvolutionaryTracei P09693.
    GeneWikii CD3G.
    GenomeRNAii 917.
    NextBioi 3794.
    PROi P09693.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P09693.
    Bgeei P09693.
    CleanExi HS_CD3G.
    Genevestigatori P09693.

    Family and domain databases

    Gene3Di 2.60.40.10. 1 hit.
    InterProi IPR015484. CD3_esu/gsu/dsu.
    IPR013783. Ig-like_fold.
    IPR003598. Ig_sub2.
    IPR003110. Phos_immunorcpt_sig_ITAM.
    [Graphical view ]
    PANTHERi PTHR10570. PTHR10570. 1 hit.
    Pfami PF02189. ITAM. 1 hit.
    [Graphical view ]
    SMARTi SM00408. IGc2. 1 hit.
    SM00077. ITAM. 1 hit.
    [Graphical view ]
    PROSITEi PS51055. ITAM_1. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Primary structure of the T3 gamma subunit of the T3/T cell antigen receptor complex deduced from cDNA sequences: evolution of the T3 gamma and delta subunits."
      Krissansen G.W., Owen M.J., Verbi W., Crumpton M.J.
      EMBO J. 5:1799-1808(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Physical linkage of three CD3 genes on human chromosome 11."
      Tunnacliffe A., Buluwela L., Rabbitts T.H.
      EMBO J. 6:2953-2957(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    4. Cited for: PROTEIN SEQUENCE OF 139-182, PHOSPHORYLATION AT SER-145 AND SER-148.
    5. "The human T3 gamma chain is phosphorylated at serine 126 in response to T lymphocyte activation."
      Davies A.A., Cantrell D.A., Hexham J.M., Parker P.J., Rothbard J., Crumpton M.J.
      J. Biol. Chem. 262:10918-10921(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION AT SER-145 AND SER-148.
    6. "A novel di-leucine motif and a tyrosine-based motif independently mediate lysosomal targeting and endocytosis of CD3 chains."
      Letourneur F., Klausner R.D.
      Cell 69:1143-1157(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: MUTAGENESIS OF LEU-153; LEU-154; TYR-160 AND LEU-163.
    7. "Brief report: primary immunodeficiency caused by mutations in the gene encoding the CD3-gamma subunit of the T-lymphocyte receptor."
      Arnaiz-Villena A., Timon M., Corell A., Perez-Aciego P., Martin-Villa J.M., Regueiro J.R.
      N. Engl. J. Med. 327:529-533(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: INVOLVEMENT IN IMD17.
    8. Cited for: INVOLVEMENT IN IMD17.
    9. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
      Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
      Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Leukemic T-cell.
    10. "Crystal structure of the human T cell receptor CD3 epsilon gamma heterodimer complexed to the therapeutic mAb OKT3."
      Kjer-Nielsen L., Dunstone M.A., Kostenko L., Ely L.K., Beddoe T., Mifsud N.A., Purcell A.W., Brooks A.G., McCluskey J., Rossjohn J.
      Proc. Natl. Acad. Sci. U.S.A. 101:7675-7680(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 23-103 IN COMPLEX WITH CD3E AND ANTIBODY, SUBUNIT, DISULFIDE BOND.

    Entry informationi

    Entry nameiCD3G_HUMAN
    AccessioniPrimary (citable) accession number: P09693
    Secondary accession number(s): Q2HIZ6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 145 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human cell differentiation molecules
      CD nomenclature of surface proteins of human leucocytes and list of entries
    2. Human chromosome 11
      Human chromosome 11: entries, gene names and cross-references to MIM
    3. Human entries with polymorphisms or disease mutations
      List of human entries with polymorphisms or disease mutations
    4. Human polymorphisms and disease mutations
      Index of human polymorphisms and disease mutations
    5. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    6. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    7. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3