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P09693 (CD3G_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 143. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (7) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Web links·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
T-cell surface glycoprotein CD3 gamma chain
Alternative name(s):
T-cell receptor T3 gamma chain
CD_antigen=CD3g
Gene names
Name:CD3G
Synonyms:T3G
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length182 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The CD3 complex mediates signal transduction.

Subunit structure

The TCR/CD3 complex of T-lymphocytes consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits of CD3 labeled gamma, delta, epsilon, zeta, and eta. Ref.10

Subcellular location

Membrane; Single-pass type I membrane protein.

Domain

A di-leucine motif and a tyrosine-based motif are individually sufficient to induce both endocytosis and delivery to lysosomes.

Involvement in disease

Immunodeficiency 17 (IMD17) [MIM:615607]: An autosomal recessive primary immunodeficiency characterized by highly variable clinical severity. Some patients have onset of severe recurrent infections in early infancy that may be lethal, whereas others may be only mildly affected or essentially asymptomatic into young adulthood. More severely affected patients may have evidence of autoimmune disease or enteropathy. The immunologic pattern is similar among patients, showing partial T-cell lymphopenia, decreased amounts of the CD3 complex, and impaired proliferative responses to T-cell receptor dependent stimuli. The phenotype in some patients is reminiscent of severe combined immunodeficiency.
Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.7 Ref.8

Sequence similarities

Contains 1 Ig-like (immunoglobulin-like) domain.

Contains 1 ITAM domain.

Ontologies

Keywords
   Cellular componentMembrane
   Coding sequence diversityPolymorphism
   DomainImmunoglobulin domain
Signal
Transmembrane
Transmembrane helix
   Molecular functionReceptor
   PTMDisulfide bond
Glycoprotein
Phosphoprotein
   Technical term3D-structure
Complete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processFc-gamma receptor signaling pathway involved in phagocytosis

Traceable author statement. Source: Reactome

T cell activation

Non-traceable author statement PubMed 12407027. Source: UniProtKB

T cell costimulation

Traceable author statement. Source: Reactome

T cell receptor signaling pathway

Traceable author statement. Source: Reactome

cell surface receptor signaling pathway

Inferred from mutant phenotype PubMed 12407027. Source: UniProtKB

establishment or maintenance of cell polarity

Inferred from mutant phenotype PubMed 12407027. Source: UniProtKB

innate immune response

Traceable author statement. Source: Reactome

protein complex assembly

Non-traceable author statement PubMed 12794121. Source: UniProtKB

protein transport

Inferred from mutant phenotype PubMed 12794121. Source: UniProtKB

regulation of immune response

Traceable author statement. Source: Reactome

regulation of lymphocyte apoptotic process

Inferred from mutant phenotype PubMed 12407027. Source: UniProtKB

   Cellular_componentT cell receptor complex

Non-traceable author statement PubMed 9485181. Source: UniProtKB

alpha-beta T cell receptor complex

Inferred from electronic annotation. Source: Ensembl

integral component of plasma membrane

Non-traceable author statement PubMed 9485181. Source: UniProtKB

plasma membrane

Traceable author statement. Source: Reactome

   Molecular_functionT cell receptor binding

Non-traceable author statement PubMed 11186279. Source: UniProtKB

protein heterodimerization activity

Inferred from physical interaction PubMed 9485181. Source: UniProtKB

receptor signaling complex scaffold activity

Non-traceable author statement PubMed 12794121. Source: UniProtKB

transmembrane signaling receptor activity

Inferred from mutant phenotype PubMed 12794121. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222
Chain23 – 182160T-cell surface glycoprotein CD3 gamma chain
PRO_0000014615

Regions

Topological domain23 – 11694Extracellular Potential
Transmembrane117 – 13721Helical; Potential
Topological domain138 – 18245Cytoplasmic Potential
Domain37 – 9458Ig-like
Domain149 – 17729ITAM
Motif153 – 1542Di-leucine motif

Amino acid modifications

Modified residue1451Phosphoserine Ref.4 Ref.5
Modified residue1481Phosphoserine Ref.4 Ref.5
Glycosylation521N-linked (GlcNAc...) Potential
Glycosylation921N-linked (GlcNAc...) Potential
Disulfide bond46 ↔ 87 Ref.10

Natural variations

Natural variant1311V → F.
Corresponds to variant rs3753058 [ dbSNP | Ensembl ].
VAR_049854

Experimental info

Mutagenesis1531L → A: Abolishes lysosomal targeting. Ref.6
Mutagenesis1531L → I: Diminished but persistent lysosomal targeting. Ref.6
Mutagenesis1541L → A: Diminished but persistent lysosomal targeting. Ref.6
Mutagenesis1541L → I: No effect. Ref.6
Mutagenesis1601Y → A: Abolishes lysosomal targeting. Ref.6
Mutagenesis1631L → A: Abolishes lysosomal targeting. Ref.6

Secondary structure

............... 182
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P09693 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: EE65C0186FB9872B

FASTA18220,469
        10         20         30         40         50         60 
MEQGKGLAVL ILAIILLQGT LAQSIKGNHL VKVYDYQEDG SVLLTCDAEA KNITWFKDGK 

        70         80         90        100        110        120 
MIGFLTEDKK KWNLGSNAKD PRGMYQCKGS QNKSKPLQVY YRMCQNCIEL NAATISGFLF 

       130        140        150        160        170        180 
AEIVSIFVLA VGVYFIAGQD GVRQSRASDK QTLLPNDQLY QPLKDREDDQ YSHLQGNQLR 


RN 

« Hide

References

« Hide 'large scale' references
[1]"Primary structure of the T3 gamma subunit of the T3/T cell antigen receptor complex deduced from cDNA sequences: evolution of the T3 gamma and delta subunits."
Krissansen G.W., Owen M.J., Verbi W., Crumpton M.J.
EMBO J. 5:1799-1808(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Physical linkage of three CD3 genes on human chromosome 11."
Tunnacliffe A., Buluwela L., Rabbitts T.H.
EMBO J. 6:2953-2957(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]"Dephosphorylation of the human T lymphocyte CD3 antigen."
Alexander D., Goris J., Marais R., Rothbard J., Merlevede W., Crumpton M.J.
Eur. J. Biochem. 181:55-65(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 139-182, PHOSPHORYLATION AT SER-145 AND SER-148.
[5]"The human T3 gamma chain is phosphorylated at serine 126 in response to T lymphocyte activation."
Davies A.A., Cantrell D.A., Hexham J.M., Parker P.J., Rothbard J., Crumpton M.J.
J. Biol. Chem. 262:10918-10921(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION AT SER-145 AND SER-148.
[6]"A novel di-leucine motif and a tyrosine-based motif independently mediate lysosomal targeting and endocytosis of CD3 chains."
Letourneur F., Klausner R.D.
Cell 69:1143-1157(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF LEU-153; LEU-154; TYR-160 AND LEU-163.
[7]"Brief report: primary immunodeficiency caused by mutations in the gene encoding the CD3-gamma subunit of the T-lymphocyte receptor."
Arnaiz-Villena A., Timon M., Corell A., Perez-Aciego P., Martin-Villa J.M., Regueiro J.R.
N. Engl. J. Med. 327:529-533(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: INVOLVEMENT IN IMD17.
[8]"Differential biological role of CD3 chains revealed by human immunodeficiencies."
Recio M.J., Moreno-Pelayo M.A., Kilic S.S., Guardo A.C., Sanal O., Allende L.M., Perez-Flores V., Mencia A., Modamio-Hoeybjoer S., Seoane E., Regueiro J.R.
J. Immunol. 178:2556-2564(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: INVOLVEMENT IN IMD17.
[9]"Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Leukemic T-cell.
[10]"Crystal structure of the human T cell receptor CD3 epsilon gamma heterodimer complexed to the therapeutic mAb OKT3."
Kjer-Nielsen L., Dunstone M.A., Kostenko L., Ely L.K., Beddoe T., Mifsud N.A., Purcell A.W., Brooks A.G., McCluskey J., Rossjohn J.
Proc. Natl. Acad. Sci. U.S.A. 101:7675-7680(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.1 ANGSTROMS) OF 23-103 IN COMPLEX WITH CD3E AND ANTIBODY, SUBUNIT, DISULFIDE BOND.
+Additional computationally mapped references.

Web resources

CD3Gbase

CD3G mutation db

Wikipedia

CD3 receptor entry

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X04145 mRNA. Translation: CAA27764.1.
X06026 expand/collapse EMBL AC list , X06027, X06028, X06029, X06030, X06031 Genomic DNA. Translation: CAA29428.1.
BC113830 mRNA. Translation: AAI13831.1.
CCDSCCDS8395.1.
PIRA25468.
RefSeqNP_000064.1. NM_000073.2.
XP_006719004.1. XM_006718941.1.
UniGeneHs.2259.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1SY6X-ray2.10A23-103[»]
DisProtDP00508.
ProteinModelPortalP09693.
SMRP09693. Positions 23-103.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid107355. 4 interactions.
IntActP09693. 1 interaction.
MINTMINT-4656150.
STRING9606.ENSP00000292144.

Chemistry

ChEMBLCHEMBL2364168.

PTM databases

PhosphoSiteP09693.

Polymorphism databases

DMDM115993.

Proteomic databases

MaxQBP09693.
PaxDbP09693.
PeptideAtlasP09693.
PRIDEP09693.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000532917; ENSP00000431445; ENSG00000160654.
GeneID917.
KEGGhsa:917.
UCSCuc001psu.2. human.

Organism-specific databases

CTD917.
GeneCardsGC11P118248.
HGNCHGNC:1675. CD3G.
HPACAB017520.
HPA038494.
MIM186740. gene.
615607. phenotype.
neXtProtNX_P09693.
Orphanet169082. Combined immunodeficiency due to CD3gamma deficiency.
PharmGKBPA26217.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG43749.
HOGENOMHOG000015287.
HOVERGENHBG005278.
InParanoidP09693.
KOK06452.
OMADRENDQY.
OrthoDBEOG7S4X7Q.
PhylomeDBP09693.
TreeFamTF335892.

Enzyme and pathway databases

ReactomeREACT_6900. Immune System.

Gene expression databases

ArrayExpressP09693.
BgeeP09693.
CleanExHS_CD3G.
GenevestigatorP09693.

Family and domain databases

Gene3D2.60.40.10. 1 hit.
InterProIPR015484. CD3_esu/gsu/dsu.
IPR013783. Ig-like_fold.
IPR003598. Ig_sub2.
IPR003110. Phos_immunorcpt_sig_ITAM.
[Graphical view]
PANTHERPTHR10570. PTHR10570. 1 hit.
PfamPF02189. ITAM. 1 hit.
[Graphical view]
SMARTSM00408. IGc2. 1 hit.
SM00077. ITAM. 1 hit.
[Graphical view]
PROSITEPS51055. ITAM_1. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSCD3G. human.
EvolutionaryTraceP09693.
GeneWikiCD3G.
GenomeRNAi917.
NextBio3794.
PROP09693.
SOURCESearch...

Entry information

Entry nameCD3G_HUMAN
AccessionPrimary (citable) accession number: P09693
Secondary accession number(s): Q2HIZ6
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: July 9, 2014
This is version 143 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human polymorphisms and disease mutations

Index of human polymorphisms and disease mutations

Human entries with polymorphisms or disease mutations

List of human entries with polymorphisms or disease mutations

Human chromosome 11

Human chromosome 11: entries, gene names and cross-references to MIM

Human cell differentiation molecules

CD nomenclature of surface proteins of human leucocytes and list of entries