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Protein

Acetylcholine receptor subunit beta

Gene

Chrnb1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

GO - Molecular functioni

  1. acetylcholine-activated cation-selective channel activity Source: MGI
  2. acetylcholine binding Source: UniProtKB
  3. acetylcholine receptor activity Source: Ensembl
  4. channel activity Source: MGI

GO - Biological processi

  1. behavioral response to nicotine Source: MGI
  2. cation transmembrane transport Source: GOC
  3. cation transport Source: MGI
  4. muscle contraction Source: MGI
  5. muscle fiber development Source: MGI
  6. neurological system process Source: MGI
  7. neuromuscular synaptic transmission Source: MGI
  8. postsynaptic membrane organization Source: MGI
  9. regulation of membrane potential Source: MGI
  10. signal transduction Source: MGI
  11. skeletal muscle contraction Source: Ensembl
  12. synaptic transmission, cholinergic Source: MGI
  13. transmembrane transport Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Acetylcholine receptor subunit beta
Gene namesi
Name:Chrnb1
Synonyms:Acrb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 11

Organism-specific databases

MGIiMGI:87890. Chrnb1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini24 – 244221ExtracellularSequence AnalysisAdd
BLAST
Transmembranei245 – 26925HelicalSequence AnalysisAdd
BLAST
Transmembranei277 – 29519HelicalSequence AnalysisAdd
BLAST
Transmembranei311 – 33222HelicalSequence AnalysisAdd
BLAST
Topological domaini333 – 469137CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei470 – 48819HelicalSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. acetylcholine-gated channel complex Source: MGI
  2. cell junction Source: UniProtKB-KW
  3. plasma membrane Source: MGI
  4. postsynaptic membrane Source: UniProtKB-SubCell
  5. synapse Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Add
BLAST
Chaini24 – 501478Acetylcholine receptor subunit betaPRO_0000000316Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi151 ↔ 165By similarity
Glycosylationi164 – 1641N-linked (GlcNAc...)Sequence Analysis
Modified residuei390 – 3901Phosphotyrosine; by Tyr-kinasesBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PRIDEiP09690.

PTM databases

PhosphoSiteiP09690.

Expressioni

Gene expression databases

BgeeiP09690.
GenevestigatoriP09690.

Interactioni

Subunit structurei

Pentamer of two alpha chains, and one each of the beta, delta, and gamma (in immature muscle) or epsilon (in mature muscle) chains.

Protein-protein interaction databases

BioGridi197935. 1 interaction.
IntActiP09690. 4 interactions.
MINTiMINT-2983098.

Structurei

3D structure databases

ProteinModelPortaliP09690.
SMRiP09690. Positions 24-341, 435-500.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG243276.
GeneTreeiENSGT00770000120477.
HOGENOMiHOG000006757.
HOVERGENiHBG003756.
InParanoidiP09690.
KOiK04812.
OMAiFIDGPNR.
OrthoDBiEOG70ZZN7.
PhylomeDBiP09690.
TreeFamiTF315605.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09690-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALGALLLLL GVLGTPLAPG ARGSEAEGQL IKKLFSNYDS SVRPAREVGD
60 70 80 90 100
RVGVSIGLTL AQLISLNEKD EEMSTKVYLD LEWTDYRLSW DPAEHDGIDS
110 120 130 140 150
LRITAESVWL PDVVLLNNND GNFDVALDIN VVVSFEGSVR WQPPGLYRSS
160 170 180 190 200
CSIQVTYFPF DWQNCTMVFS SYSYDSSEVS LKTGLDPEGE ERQEVYIHEG
210 220 230 240 250
TFIENGQWEI IHKPSRLIQL PGDQRGGKEG HHEEVIFYLI IRRKPLFYLV
260 270 280 290 300
NVIAPCILIT LLAIFVFYLP PDAGEKMGLS IFALLTLTVF LLLLADKVPE
310 320 330 340 350
TSLAVPIIIK YLMFTMVLVT FSVILSVVVL NLHHRSPHTH QMPFWVRQIF
360 370 380 390 400
IHKLPPYLGL KRPKPERDQL PEPHHSLSPR SGWGRGTDEY FIRKPPSDFL
410 420 430 440 450
FPKLNRFQPE SSAPDLRRFI DGPTRAVGLP QELREVISSI SYMARQLQEQ
460 470 480 490 500
EDHDALKEDW QFVAMVVDRL FLWTFIVFTS VGTLVIFLDA TYHLPPPEPF

P
Length:501
Mass (Da):56,931
Last modified:July 1, 1989 - v1
Checksum:i787BDDA90EBB0EF2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14537 mRNA. Translation: AAA37154.1.
J04699 Genomic DNA. Translation: AAA37156.1.
AK087554 mRNA. Translation: BAC39924.1.
AL603707 Genomic DNA. Translation: CAI51959.1.
CCDSiCCDS24910.1.
PIRiA33358. A25338.
RefSeqiNP_033731.3. NM_009601.4.
UniGeneiMm.86425.

Genome annotation databases

EnsembliENSMUST00000045971; ENSMUSP00000047270; ENSMUSG00000041189.
GeneIDi11443.
KEGGimmu:11443.
UCSCiuc007jrq.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14537 mRNA. Translation: AAA37154.1.
J04699 Genomic DNA. Translation: AAA37156.1.
AK087554 mRNA. Translation: BAC39924.1.
AL603707 Genomic DNA. Translation: CAI51959.1.
CCDSiCCDS24910.1.
PIRiA33358. A25338.
RefSeqiNP_033731.3. NM_009601.4.
UniGeneiMm.86425.

3D structure databases

ProteinModelPortaliP09690.
SMRiP09690. Positions 24-341, 435-500.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi197935. 1 interaction.
IntActiP09690. 4 interactions.
MINTiMINT-2983098.

Chemistry

BindingDBiP09690.
ChEMBLiCHEMBL3137264.
GuidetoPHARMACOLOGYi471.

PTM databases

PhosphoSiteiP09690.

Proteomic databases

PRIDEiP09690.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000045971; ENSMUSP00000047270; ENSMUSG00000041189.
GeneIDi11443.
KEGGimmu:11443.
UCSCiuc007jrq.2. mouse.

Organism-specific databases

CTDi1140.
MGIiMGI:87890. Chrnb1.

Phylogenomic databases

eggNOGiNOG243276.
GeneTreeiENSGT00770000120477.
HOGENOMiHOG000006757.
HOVERGENiHBG003756.
InParanoidiP09690.
KOiK04812.
OMAiFIDGPNR.
OrthoDBiEOG70ZZN7.
PhylomeDBiP09690.
TreeFamiTF315605.

Miscellaneous databases

NextBioi278748.
PROiP09690.
SOURCEiSearch...

Gene expression databases

BgeeiP09690.
GenevestigatoriP09690.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "A universal oligonucleotide probe for acetylcholine receptor genes. Selection and sequencing of cDNA clones for the mouse muscle beta subunit."
    Buonanno A., Mudd J., Shah V., Merlie J.P.
    J. Biol. Chem. 261:16451-16458(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Isolation and characterization of the beta and epsilon subunit genes of mouse muscle acetylcholine receptor."
    Buonanno A., Mudd J., Merlie J.P.
    J. Biol. Chem. 264:7611-7616(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Eye.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.

Entry informationi

Entry nameiACHB_MOUSE
AccessioniPrimary (citable) accession number: P09690
Secondary accession number(s): Q5F292
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: February 4, 2015
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.