ID   G3PA_SINAL              Reviewed;         233 AA.
AC   P09672;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JUL-1989, sequence version 1.
DT   16-JUN-2009, entry version 69.
DE   RecName: Full=Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic;
DE            EC=1.2.1.13;
DE   AltName: Full=NADP-dependent glyceraldehydephosphate dehydrogenase subunit A;
DE   Flags: Fragment;
GN   Name=GAPA;
OS   Sinapis alba (White mustard) (Brassica hirta).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliophyta; eudicotyledons; core eudicotyledons;
OC   rosids; eurosids II; Brassicales; Brassicaceae; Sinapis.
OX   NCBI_TaxID=3728;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RX   MEDLINE=87004643; PubMed=3530755;
RX   DOI=10.1111/j.1432-1033.1986.tb09871.x;
RA   Martin W.F., Cerff R.;
RT   "Prokaryotic features of a nucleus-encoded enzyme. cDNA sequences for
RT   chloroplast and cytosolic glyceraldehyde-3-phosphate dehydrogenases
RT   from mustard (Sinapis alba).";
RL   Eur. J. Biochem. 159:323-331(1986).
CC   -!- CATALYTIC ACTIVITY: D-glyceraldehyde 3-phosphate + phosphate +
CC       NADP(+) = 3-phospho-D-glyceroyl phosphate + NADPH.
CC   -!- PATHWAY: Carbohydrate biosynthesis; Calvin cycle.
CC   -!- SUBUNIT: Tetramer of either four A chains (GAPDH 2) or two A and
CC       two B chains (GAPDH 1) (By similarity).
CC   -!- SUBCELLULAR LOCATION: Plastid, chloroplast.
CC   -!- MISCELLANEOUS: Plants contain three forms of GAPDH: a cytosolic
CC       form which participates in glycolysis and two chloroplast forms
CC       which participates in photosynthesis. These three forms are
CC       encoded by distinct genes.
CC   -!- SIMILARITY: Belongs to the glyceraldehyde-3-phosphate
CC       dehydrogenase family.
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DR   EMBL; X04302; CAA27845.1; -; mRNA.
DR   PIR; B24796; B24796.
DR   HSSP; P19866; 1NBO.
DR   SMR; P09672; 1-233.
DR   BRENDA; 1.2.1.13; 20822.
DR   GO; GO:0009507; C:chloroplast; IEA:UniProtKB-SubCell.
DR   GO; GO:0047100; F:glyceraldehyde-3-phosphate dehydrogenase (N...; IEA:EC.
DR   GO; GO:0004365; F:glyceraldehyde-3-phosphate dehydrogenase (p...; IEA:InterPro.
DR   GO; GO:0051287; F:NAD or NADH binding; IEA:InterPro.
DR   GO; GO:0006006; P:glucose metabolic process; IEA:InterPro.
DR   GO; GO:0055114; P:oxidation reduction; IEA:UniProtKB-KW.
DR   GO; GO:0019253; P:reductive pentose-phosphate cycle; IEA:UniProtKB-KW.
DR   InterPro; IPR000173; GlycerAld_3-P_DH.
DR   PANTHER; PTHR10836; GAP_DH; 1.
DR   Pfam; PF02800; Gp_dh_C; 1.
DR   Pfam; PF00044; Gp_dh_N; 1.
DR   PRINTS; PR00078; G3PDHDRGNASE.
DR   PROSITE; PS00071; GAPDH; 1.
PE   2: Evidence at transcript level;
KW   Calvin cycle; Chloroplast; NADP; Oxidoreductase; Plastid.
FT   CHAIN        <1    233       Glyceraldehyde-3-phosphate dehydrogenase
FT                                A, chloroplastic.
FT                                /FTId=PRO_0000145617.
FT   REGION       49     51       Glyceraldehyde 3-phosphate binding (By
FT                                similarity).
FT   REGION      108    109       Glyceraldehyde 3-phosphate binding (By
FT                                similarity).
FT   ACT_SITE     50     50       Nucleophile (By similarity).
FT   BINDING      80     80       Glyceraldehyde 3-phosphate (By
FT                                similarity).
FT   BINDING      95     95       Glyceraldehyde 3-phosphate (By
FT                                similarity).
FT   BINDING     131    131       Glyceraldehyde 3-phosphate (By
FT                                similarity).
FT   BINDING     213    213       NADP (By similarity).
FT   SITE         77     77       Activates thiol group during catalysis
FT                                (By similarity).
FT   NON_TER       1      1
SQ   SEQUENCE   233 AA;  25103 MW;  EE1502C53AC556F3 CRC64;
     DREGAGKHIQ AGAKKVLITA PGKGDIPTYV VGVNAELYSH EDTIISNASC TTNCLAPFVK
     VLDQKFGIIK GTMTTTHSYT GDQRLLDASH RDLRRARAAA LNIVPTSTGA AKAVALVLPN
     LKGKLNGIAL RVPTPNVSVV DLVVQVSKKT FAEEVNAAFR DAAEKELKGI LDVCDEPLVS
     VDFRCSDVSS TIDSSLTMVM GDDMVKVIAW YDNEWGYSQR VVDLADIVAN NWK
//