Reviewed, UniProtKB/Swiss-Prot P09672 (G3PA_SINAL)

Names and origin

Protein names

Recommended name:
    Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic
    EC=1.2.1.13
Alternative name(s):
    NADP-dependent glyceraldehydephosphate dehydrogenase subunit A

Gene names

Name:

GAPA

Organism

Sinapis alba (White mustard) (Brassica hirta)

Taxonomic identifier

3728 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Sinapis

Protein attributes

Sequence length	233 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	D-glyceraldehyde 3-phosphate + phosphate + NADP+ = 3-phospho-D-glyceroyl phosphate + NADPH.
Pathway	Carbohydrate biosynthesis; Calvin cycle.
Subunit structure	Tetramer of either four A chains (GAPDH 2) or two A and two B chains (GAPDH 1) By similarity.
Subcellular location	Plastid › chloroplast.
Miscellaneous	Plants contain three forms of GAPDH: a cytosolic form which participates in glycolysis and two chloroplast forms which participates in photosynthesis. These three forms are encoded by distinct genes.
Sequence similarities	Belongs to the glyceraldehyde-3-phosphate dehydrogenase family.

Ontologies

Keywords
Biological process	Calvin cycle
Cellular component	Chloroplast Plastid
Ligand	NADP
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	glucose metabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW reductive pentose-phosphate cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	NAD or NADH binding Inferred from electronic annotation. Source: InterPro glyceraldehyde-3-phosphate dehydrogenase (NADP+) (phosphorylating) activity Inferred from electronic annotation. Source: EC glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 233	›233	Glyceraldehyde-3-phosphate dehydrogenase A, chloroplastic		PRO_0000145617
Regions
	Region	49 – 51	3	Glyceraldehyde 3-phosphate binding By similarity
	Region	108 – 109	2	Glyceraldehyde 3-phosphate binding By similarity
Sites
	Active site	50	1	Nucleophile By similarity
	Binding site	80	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	95	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	131	1	Glyceraldehyde 3-phosphate By similarity
	Binding site	213	1	NADP By similarity
	Site	77	1	Activates thiol group during catalysis By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P09672-1 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: EE1502C53AC556F3	FASTA	233	25,103
	10 20 30 40 50 60 DREGAGKHIQ AGAKKVLITA PGKGDIPTYV VGVNAELYSH EDTIISNASC TTNCLAPFVK 70 80 90 100 110 120 VLDQKFGIIK GTMTTTHSYT GDQRLLDASH RDLRRARAAA LNIVPTSTGA AKAVALVLPN 130 140 150 160 170 180 LKGKLNGIAL RVPTPNVSVV DLVVQVSKKT FAEEVNAAFR DAAEKELKGI LDVCDEPLVS 190 200 210 220 230 VDFRCSDVSS TIDSSLTMVM GDDMVKVIAW YDNEWGYSQR VVDLADIVAN NWK

References

[1]	"Prokaryotic features of a nucleus-encoded enzyme. cDNA sequences for chloroplast and cytosolic glyceraldehyde-3-phosphate dehydrogenases from mustard (Sinapis alba)." Martin W.F., Cerff R. Eur. J. Biochem. 159:323-331(1986) [PubMed: 3530755] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X04302 mRNA. Translation: CAA27845.1.
PIR	B24796.
3D structure databases
HSSP	HSSP built from PDB template 1NBO based on UniProtKB P19866.
SMR	P09672. Positions 1-233.
ModBase	Search...
Enzyme and pathway databases
BRENDA	1.2.1.13. 20822.
Family and domain databases
InterPro	IPR000173. GlycerAld_3-P_DH. [Graphical view]
PANTHER	PTHR10836. GAP_DH. 1 hit.
Pfam	PF02800. Gp_dh_C. 1 hit. PF00044. Gp_dh_N. 1 hit. [Graphical view]
PRINTS	PR00078. G3PDHDRGNASE.
PROSITE	PS00071. GAPDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

G3PA_SINAL

Accession

Primary (citable) accession number: P09672

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	June 16, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)