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Protein

Acetylcholine receptor subunit epsilon

Gene

Chrne

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

GO - Molecular functioni

  • acetylcholine-activated cation-selective channel activity Source: RGD

GO - Biological processi

  • cation transmembrane transport Source: GOC
  • cation transport Source: RGD
  • skeletal muscle contraction Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Acetylcholine receptor subunit epsilon
Gene namesi
Name:Chrne
Synonyms:Achre
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2353. Chrne.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini21 – 239219ExtracellularSequence analysisAdd
BLAST
Transmembranei240 – 26425HelicalSequence analysisAdd
BLAST
Topological domaini265 – 2728CytoplasmicSequence analysis
Transmembranei273 – 29119HelicalSequence analysisAdd
BLAST
Topological domaini292 – 30615ExtracellularSequence analysisAdd
BLAST
Transmembranei307 – 32822HelicalSequence analysisAdd
BLAST
Topological domaini329 – 457129CytoplasmicSequence analysisAdd
BLAST
Transmembranei458 – 48124HelicalSequence analysisAdd
BLAST
Topological domaini482 – 49413ExtracellularSequence analysisAdd
BLAST

GO - Cellular componenti

  • acetylcholine-gated channel complex Source: RGD
  • cell junction Source: UniProtKB-KW
  • postsynaptic membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL4961.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020By similarityAdd
BLAST
Chaini21 – 494474Acetylcholine receptor subunit epsilonPRO_0000000331Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi86 – 861N-linked (GlcNAc...)Sequence analysis
Disulfide bondi148 ↔ 162By similarity
Glycosylationi161 – 1611N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

PaxDbiP09660.
PRIDEiP09660.

Interactioni

Subunit structurei

Pentamer of two alpha chains, and one each of the beta, delta, and gamma (in immature muscle) or epsilon (in mature muscle) chains.

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000005050.

Chemistry

BindingDBiP09660.

Structurei

3D structure databases

ProteinModelPortaliP09660.
SMRiP09660. Positions 22-337.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3645. Eukaryota.
ENOG410XQGR. LUCA.
HOGENOMiHOG000006757.
HOVERGENiHBG003756.
InParanoidiP09660.
KOiK04817.
PhylomeDBiP09660.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 2 hits.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09660-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTMALLGTLL LLALFGRSQG KNEELSLYHH LFDNYDPECR PVRRPEDTVT
60 70 80 90 100
ITLKVTLTNL ISLNEKEETL TTSVWIGIEW QDYRLNFSKD DFAGVEILRV
110 120 130 140 150
PSEHVWLPEI VLENNIDGQF GVAYDCNVLV YEGGSVSWLP PAIYRSTCAV
160 170 180 190 200
EVTYFPFDWQ NCSLIFRSQT YNAEEVELIF AVDDDGNAIN KIDIDTAAFT
210 220 230 240 250
ENGEWAIDYC PGMIRHYEGG STEDPGETDV IYTLIIRRKP LFYVINIIVP
260 270 280 290 300
CVLISGLVLL AYFLPAQAGG QKCTVSINVL LAQTVFLFLI AQKIPETSLS
310 320 330 340 350
VPLLGRYLIF VMVVATLIVM NCVIVLNVSL RTPTTHATSP RLRQILLELL
360 370 380 390 400
PRLLGLSPPP EDPGAASPAR RASSVGILLR AEELILKKPR SELVFEGQRH
410 420 430 440 450
RHGTWTAAAL CQNLGAAAPE VRCCVDAVNF VAESTRDQEA TGEELSDWVR
460 470 480 490
MGKALDNVCF WAALVLFSVG STLIFLGGYF NQVPDLPYPP CIQP
Length:494
Mass (Da):54,948
Last modified:May 1, 1992 - v2
Checksum:i6476E4C938A6A9B6
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti391 – 3922SE → R in CAA31628 (PubMed:3205730).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74836 mRNA. Translation: CAA52830.1.
X13252 mRNA. Translation: CAA31628.1.
Z23062 Genomic DNA. Translation: CAA80597.1.
X06365 Genomic DNA. Translation: CAA29663.1.
PIRiS01959. ACRTE.
RefSeqiNP_058890.1. NM_017194.1.
UniGeneiRn.10301.

Genome annotation databases

GeneIDi29422.
KEGGirno:29422.
UCSCiRGD:2353. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74836 mRNA. Translation: CAA52830.1.
X13252 mRNA. Translation: CAA31628.1.
Z23062 Genomic DNA. Translation: CAA80597.1.
X06365 Genomic DNA. Translation: CAA29663.1.
PIRiS01959. ACRTE.
RefSeqiNP_058890.1. NM_017194.1.
UniGeneiRn.10301.

3D structure databases

ProteinModelPortaliP09660.
SMRiP09660. Positions 22-337.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000005050.

Chemistry

BindingDBiP09660.
ChEMBLiCHEMBL4961.

Proteomic databases

PaxDbiP09660.
PRIDEiP09660.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi29422.
KEGGirno:29422.
UCSCiRGD:2353. rat.

Organism-specific databases

CTDi1145.
RGDi2353. Chrne.

Phylogenomic databases

eggNOGiKOG3645. Eukaryota.
ENOG410XQGR. LUCA.
HOGENOMiHOG000006757.
HOVERGENiHBG003756.
InParanoidiP09660.
KOiK04817.
PhylomeDBiP09660.

Miscellaneous databases

PROiP09660.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 2 hits.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Primary structure and functional expression of the alpha-, beta-, gamma-, delta- and epsilon-subunits of the acetylcholine receptor from rat muscle."
    Witzemann V., Stein E., Barg B., Konno T., Koenen M., Kues W., Criado M., Hofmann M., Sakmann B.
    Eur. J. Biochem. 194:437-448(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE.
    Tissue: Muscle.
  2. "Nucleotide sequence of the rat muscle acetylcholine receptor epsilon-subunit."
    Criado M., Witzemann V., Koenen M., Sakmann B.
    Nucleic Acids Res. 16:10920-10920(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
  3. "Calcium-dependent regulation of rat and chick muscle nicotinic acetylcholine receptor (nAChR) gene expression."
    Walke W., Staple J., Adams L., Gnegy M., Chahine K., Goldman D.
    J. Biol. Chem. 269:19447-19456(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-23.
    Strain: Fischer.
    Tissue: Liver.
  4. "Differential regulation of muscle acetylcholine receptor gamma- and epsilon-subunit mRNAs."
    Witzemann V., Barg B., Nishikawa Y., Sakmann B., Numa S.
    FEBS Lett. 223:104-112(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 202-305.
    Strain: Sprague-Dawley.

Entry informationi

Entry nameiACHE_RAT
AccessioniPrimary (citable) accession number: P09660
Secondary accession number(s): Q6LAD4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: May 1, 1992
Last modified: June 8, 2016
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.