P09658 (RBSC_CUPNE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribulose bisphosphate carboxylase small chain, chromosomal
Short name=RuBisCO small subunit
EC=4.1.1.39

Gene names

Name:	cbxSC
Synonyms:	cbbS, cfxSC, rbcS

Organism

Cupriavidus necator (Alcaligenes eutrophus) (Ralstonia eutropha)

Taxonomic identifier

106590 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Betaproteobacteria › Burkholderiales › Burkholderiaceae › Cupriavidus

Protein attributes

Sequence length	139 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	RuBisCO catalyzes two reactions: the carboxylation of D-ribulose 1,5-bisphosphate, the primary event in carbon dioxide fixation, as well as the oxidative fragmentation of the pentose substrate. Both reactions occur simultaneously and in competition at the same active site.
Catalytic activity	2 3-phospho-D-glycerate + 2 H+ = D-ribulose 1,5-bisphosphate + CO2 + H2O. 3-phospho-D-glycerate + 2-phosphoglycolate = D-ribulose 1,5-bisphosphate + O2.
Subunit structure	8 large chains + 8 small chains.
Sequence similarities	Belongs to the RuBisCO small chain family.

Ontologies

Keywords
Biological process	Calvin cycle Carbon dioxide fixation
Molecular function	Lyase Monooxygenase Oxidoreductase
Technical term	3D-structure
Gene Ontology (GO)
Biological_process	reductive pentose-phosphate cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	monooxygenase activity Inferred from electronic annotation. Source: UniProtKB-KW ribulose-bisphosphate carboxylase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 139

139

Ribulose bisphosphate carboxylase small chain, chromosomal

PRO_0000198607

Secondary structure

1

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139

Helix Strand Turn

Details...

Turn

8 – 10

3

Helix

16 – 28

13

Beta strand

32 – 39

8

Beta strand

49 – 53

5

Helix

61 – 74

14

Beta strand

76 – 85

10

Turn

87 – 89

3

Beta strand

92 – 97

6

Helix

102 – 104

3

Beta strand

110 – 115

6

Beta strand

121 – 126

6

Sequences

	Sequence		Length	Mass (Da)
	P09658 [UniParc]. Last modified July 15, 1999. Version 2. Checksum: BDD2679F24897A1C	FASTA	139	16,143
	10 20 30 40 50 60 MRITQGTFSF LPELTDEQIT KQLEYCLNQG WAVGLEYTDD PHPRNTYWEM FGLPMFDLRD 70 80 90 100 110 120 AAGILMEINN ARNTFPNHYI RVTAFDSTHT VESVVMSFIV NRPADEPGFR LVRQEEPGRT 130 LRYSIESYAV QARPEGSRY

References

[1]	"Sequence analysis of the Alcaligenes eutrophus chromosomally encoded ribulose bisphosphate carboxylase large and small subunit genes and their gene products." Andersen K., Caton J. J. Bacteriol. 169:4547-4558(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 17707 / LMG 1207 / Stanier 345 / H-20-R.
[2]	Andersen K. Submitted (AUG-1998) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"The crystal structure of rubisco from Alcaligenes eutrophus reveals a novel central eight-stranded beta-barrel formed by beta-strands from four subunits." Hansen S., Vollan V.B., Hough E., Andersen K. J. Mol. Biol. 288:609-621(1999) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS). Strain: ATCC 17707 / LMG 1207 / Stanier 345 / H-20-R.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M17744 Genomic DNA. Translation: AAC28130.1.

PIR

RKALSE. B26954.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1BXN	X-ray	2.70	I/J/K/L	1-139	[»]

ProteinModelPortal

P09658.

SMR

P09658. Positions 1-129.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-6120N.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Family and domain databases

Gene3D

3.30.190.10. 1 hit.

InterPro

IPR000894. RuBisCO_sc_dom.
[Graphical view]

Pfam

PF00101. RuBisCO_small. 1 hit.
[Graphical view]

SMART

SM00961. RuBisCO_small. 1 hit.
[Graphical view]

SUPFAM

SSF55239. RuBisCO_small. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P09658.

Entry information

Entry name

RBSC_CUPNE

Accession

Primary (citable) accession number: P09658

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 15, 1999
Last modified:	April 3, 2013
This is version 83 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program