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Protein

Serine protease inhibitor Kazal-type 1-like

Gene

Spink1l

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Serine protease inhibitor which exhibits anti-trypsin activity (PubMed:3202973, PubMed:2110056). In the pancreas, protects against trypsin-catalyzed premature activation of zymogens (By similarity).By similarity2 Publications
In the male reproductive tract, binds to sperm heads where it modulates sperm capacitance by inhibiting calcium uptake and nitrogen oxide (NO) production.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei41 – 422Reactive bond for trypsinBy similarity
Sitei43 – 442Necessary for sperm bindingBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Protease inhibitor, Serine protease inhibitor

Protein family/group databases

MEROPSiI01.047.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine protease inhibitor Kazal-type 1-likeImported
Alternative name(s):
Calcium transport inhibitor
Caltrin
Pancreatic secretory trypsin inhibitor II
Short name:
PSTI-II
Gene namesi
Name:Spink1lImported
Synonyms:Spink3Imported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 18

Organism-specific databases

RGDi708468. Spink1l.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 23233 PublicationsAdd
BLAST
Chaini24 – 7956Serine protease inhibitor Kazal-type 1-likePRO_0000016559Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi32 ↔ 61PROSITE-ProRule annotation
Disulfide bondi39 ↔ 58PROSITE-ProRule annotation
Disulfide bondi47 ↔ 79PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP09656.
PRIDEiP09656.

Expressioni

Tissue specificityi

Seminal vesicle.1 Publication

Gene expression databases

GenevisibleiP09656. RN.

Interactioni

Protein-protein interaction databases

IntActiP09656. 1 interaction.
STRINGi10116.ENSRNOP00000017969.

Structurei

3D structure databases

ProteinModelPortaliP09656.
SMRiP09656. Positions 26-79.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 7954Kazal-likePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 Kazal-like domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410KCWT. Eukaryota.
ENOG410ZBM0. LUCA.
GeneTreeiENSGT00530000064228.
HOGENOMiHOG000090244.
HOVERGENiHBG006182.
InParanoidiP09656.
OMAiREATCTS.
OrthoDBiEOG7BW0M2.
PhylomeDBiP09656.

Family and domain databases

InterProiIPR002350. Kazal_dom.
IPR001239. Prot_inh_Kazal-m.
[Graphical view]
PfamiPF00050. Kazal_1. 1 hit.
[Graphical view]
PRINTSiPR00290. KAZALINHBTR.
SMARTiSM00280. KAZAL. 1 hit.
[Graphical view]
PROSITEiPS00282. KAZAL_1. 1 hit.
PS51465. KAZAL_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09656-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKVAIIFLLS ALALLNLAGN TTAKVIGKKA NCPNTLVGCP RDYDPVCGTD
60 70
GKTYANECIL CFENRKFGTS IRIQRRGLC
Length:79
Mass (Da):8,565
Last modified:February 1, 1991 - v2
Checksum:i6782EBBB531F677C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27883 mRNA. Translation: AAA41976.1. Sequence problems.
D11325 Genomic DNA. Translation: BAA01945.1.
PIRiB33292. TIRT2.
RefSeqiNP_690919.1. NM_152936.1.
UniGeneiRn.144683.

Genome annotation databases

EnsembliENSRNOT00000017969; ENSRNOP00000017969; ENSRNOG00000013410.
GeneIDi266602.
KEGGirno:266602.
UCSCiRGD:708468. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M27883 mRNA. Translation: AAA41976.1. Sequence problems.
D11325 Genomic DNA. Translation: BAA01945.1.
PIRiB33292. TIRT2.
RefSeqiNP_690919.1. NM_152936.1.
UniGeneiRn.144683.

3D structure databases

ProteinModelPortaliP09656.
SMRiP09656. Positions 26-79.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP09656. 1 interaction.
STRINGi10116.ENSRNOP00000017969.

Protein family/group databases

MEROPSiI01.047.

Proteomic databases

PaxDbiP09656.
PRIDEiP09656.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000017969; ENSRNOP00000017969; ENSRNOG00000013410.
GeneIDi266602.
KEGGirno:266602.
UCSCiRGD:708468. rat.

Organism-specific databases

CTDi6690.
RGDi708468. Spink1l.

Phylogenomic databases

eggNOGiENOG410KCWT. Eukaryota.
ENOG410ZBM0. LUCA.
GeneTreeiENSGT00530000064228.
HOGENOMiHOG000090244.
HOVERGENiHBG006182.
InParanoidiP09656.
OMAiREATCTS.
OrthoDBiEOG7BW0M2.
PhylomeDBiP09656.

Miscellaneous databases

PROiP09656.

Gene expression databases

GenevisibleiP09656. RN.

Family and domain databases

InterProiIPR002350. Kazal_dom.
IPR001239. Prot_inh_Kazal-m.
[Graphical view]
PfamiPF00050. Kazal_1. 1 hit.
[Graphical view]
PRINTSiPR00290. KAZALINHBTR.
SMARTiSM00280. KAZAL. 1 hit.
[Graphical view]
PROSITEiPS00282. KAZAL_1. 1 hit.
PS51465. KAZAL_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "On the cDNA's for two types of rat pancreatic secretory trypsin inhibitor."
    Horii A., Tomita N., Yokouchi H., Doi S., Uda K., Ogawa M., Mori T., Matsubara K.
    Biochem. Biophys. Res. Commun. 162:151-159(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Pancreas.
  2. "Molecular cloning and characterization of genes encoding rat pancreatic cholecystokinin (CCK)-releasing peptide (monitor peptide) and pancreatic secretory trypsin inhibitor (PSTI)."
    Tsuzuki S., Miura Y., Fushiki T., Oomori T., Satoh T., Natori Y., Sugimoto E.
    Biochim. Biophys. Acta 1132:199-202(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Purification, characterization and amino-acid sequencing of two pancreatic secretory trypsin inhibitors in rat pancreatic juice."
    Uda K., Ogawa M., Shibita T., Murata A., Mori T., Kikuchi N., Yoshida N., Tsunasawa S., Sakiyama F.
    Biol. Chem. Hoppe-Seyler 369:55-61(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 24-79, FUNCTION.
    Strain: Wistar.
    Tissue: Pancreas.
  4. "A low-molecular-mass Kazal-type protease inhibitor isolated from rat hepatocytes is identical to rat pancreatic secretory trypsin inhibitor II. Purification and amino acid sequence."
    Kido H., Yokogoshi Y., Katunuma N.
    Eur. J. Biochem. 188:501-506(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 24-79.
    Tissue: Hepatocyte.
  5. "Purification, structure, and characterization of caltrin proteins from seminal vesicle of the rat and mouse."
    Coronel C.E., Winnica D.E., Novella M.L., Lardy H.A.
    J. Biol. Chem. 267:20909-20915(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 24-79, FUNCTION, TISSUE SPECIFICITY.
    Tissue: Seminal vesicle.

Entry informationi

Entry nameiISK1L_RAT
AccessioniPrimary (citable) accession number: P09656
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: February 1, 1991
Last modified: July 6, 2016
This is version 130 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.