Reviewed, UniProtKB/Swiss-Prot P09650 (MCPT1_RAT)

Names and origin

Protein names

Recommended name:
    Mast cell protease 1
    EC=3.4.21.39
Alternative name(s):
    Mast cell protease I
    RMCP-1
    RMCP-I
    Chymase
    Chymotrysin-like protease
      Short name=CLIP protein

Gene names

Name:

Mcpt1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion. May participate in generating perivascular beta-protein which ultimately aggregates into beta-amyloid deposits. Ref.5
Catalytic activity	Preferential cleavage: Phe-|-Xaa > Tyr-|-Xaa > Trp-|-Xaa > Leu-|-Xaa.
Subcellular location	Secreted By similarity. Cytoplasmic granule By similarity. Note: Mast cell granules By similarity.
Sequence similarities	Belongs to the peptidase S1 family. Granzyme subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	serine-type endopeptidase activity Ref.1 Non-traceable author statement. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Potential
	Propeptide	19 – 20	2	Activation peptide Ref.5 Ref.3 Ref.4		PRO_0000027439
	Chain	21 – 260	240	Mast cell protease 1		PRO_0000027440
Regions
	Domain	21 – 244	224	Peptidase S1
Sites
	Active site	65	1	Charge relay system By similarity
	Active site	109	1	Charge relay system By similarity
	Active site	202	1	Charge relay system By similarity
Amino acid modifications
	Disulfide bond	50 ↔ 66		By similarity
	Disulfide bond	143 ↔ 208		By similarity
	Disulfide bond	174 ↔ 187		By similarity
Experimental info
	Sequence conflict	56 – 58	3	TRQ → NRN AA sequence Ref.3
	Sequence conflict	70 – 71	2	ET → NE AA sequence Ref.3
	Sequence conflict	91 – 94	4	KVEK → ALEI AA sequence Ref.5

Sequences

	Sequence		Length	Mass (Da)
	P09650-1 [UniParc]. Last modified November 1, 1997. Version 3. Checksum: 076278089C1F8A96	FASTA	260	28,617
	10 20 30 40 50 60 MQALLFLMAL LLPSGAGAEE IIGGVESRPH SRPYMAHLEI TTERGYKATC GGFLVTRQFV 70 80 90 100 110 120 MTAAHCKGRE TTVTLGVHDV SKTESTQQKI KVEKQIVHPN YNFYSNLHDI MLLKLQKKAK 130 140 150 160 170 180 VTPAVDVIPL PQPSDFLKPG KMCRAAGWGQ TGVTKPTSNT LREVKQRIMD KEACKNYFHY 190 200 210 220 230 240 NYNFQVCVGS PRKIRSAYKG DSGGPLVCAG VAHGIVSYGR GDAKPPAVFT RISPYVPWIN 250 260 KVIKGKDLTS LSLHESESPS

References

[1]	"Secretory granule proteases in rat mast cells. Cloning of 10 different serine proteases and a carboxypeptidase A from various rat mast cell populations." Lutzelschwab C., Pejler G., Aveskogh M., Hellman L. J. Exp. Med. 185:13-29(1997) [PubMed: 8996238] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Mast cell.
[2]	"Molecular cloning of rat mast cell protease 1 and development of specific probes for its gene transcript." Rouleau A., Garbarg M., Schwartz J.C., Ruat M. Biochem. Biophys. Res. Commun. 199:593-602(1994) [PubMed: 8135800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 10-260.
[3]	"Amino acid sequence of rat mast cell protease I (chymase)." le Trong H., Parmelee D.C., Walsh K.A., Neurath H., Woodbury R.G. Biochemistry 26:6988-6994(1987) [PubMed: 3122823] [Abstract] Cited for: PROTEIN SEQUENCE OF 21-247.
[4]	"A major serine protease in rat skeletal muscle: evidence for its mast cell origin." Woodbury R.G., Everitt M., Sanada Y., Katunuma N., Lagunoff D., Neurath H. Proc. Natl. Acad. Sci. U.S.A. 75:5311-5313(1978) [PubMed: 103093] [Abstract] Cited for: PROTEIN SEQUENCE OF 21-73.
[5]	"Identification of a chymotrypsin-like mast cell protease in rat brain capable of generating the N-terminus of the Alzheimer amyloid beta-protein." Nelson R.B., Siman R., Iqbal M.A., Potter H. J. Neurochem. 61:567-577(1993) [PubMed: 8336143] [Abstract] Cited for: PROTEIN SEQUENCE OF 21-34; 91-101 AND 232-238, FUNCTION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U67915 mRNA. Translation: AAB48268.1. S69206 mRNA. Translation: AAB30269.1.
IPI	IPI00188478.
PIR	JC2125.
UniGene	Rn.202971
3D structure databases
HSSP	HSSP built from PDB template 1NN6 based on UniProtKB P23946.
ModBase	Search...
Protein family/group databases
MEROPS	S01.149.
Genome annotation databases
Ensembl	ENSRNOG00000020633. Rattus norvegicus. [Contig view]
Organism-specific databases
RGD	3062. Mcpt1.
Phylogenomic databases
HOVERGEN	P09650.
OMA	P09650. YVPWINK.
Enzyme and pathway databases
BRENDA	3.4.21.39. 248.
Gene expression databases
ArrayExpress	P09650.
GermOnline	ENSRNOG00000020633. Rattus norvegicus.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MCPT1_RAT

Accession

Primary (citable) accession number: P09650

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	November 1, 1997
Last modified:	June 16, 2009
This is version 84 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)