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P09650

- MCPT1_RAT

UniProt

P09650 - MCPT1_RAT

Protein

Mast cell protease 1

Gene

Mcpt1

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 3 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion. May participate in generating perivascular beta-protein which ultimately aggregates into beta-amyloid deposits.1 Publication

    Catalytic activityi

    Preferential cleavage: Phe-|-Xaa > Tyr-|-Xaa > Trp-|-Xaa > Leu-|-Xaa.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei65 – 651Charge relay systemBy similarity
    Active sitei109 – 1091Charge relay systemBy similarity
    Active sitei202 – 2021Charge relay systemBy similarity

    GO - Molecular functioni

    1. serine-type endopeptidase activity Source: RGD

    GO - Biological processi

    1. regulation of angiotensin levels in blood Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase, Protease, Serine protease

    Protein family/group databases

    MEROPSiS01.149.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Mast cell protease 1 (EC:3.4.21.39)
    Short name:
    rMCP-1
    Alternative name(s):
    Chymase
    Chymotrypsin-like protease
    Short name:
    CLIP protein
    Mast cell protease I
    Short name:
    rMCP-I
    Gene namesi
    Name:Mcpt1
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 15

    Organism-specific databases

    RGDi3062. Mcpt1.

    Subcellular locationi

    Secreted By similarity. Cytoplasmic granule By similarity
    Note: Mast cell granules.By similarity

    GO - Cellular componenti

    1. extracellular region Source: UniProtKB-SubCell
    2. intracellular Source: Ensembl

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 1818Sequence AnalysisAdd
    BLAST
    Propeptidei19 – 202Activation peptide3 PublicationsPRO_0000027439
    Chaini21 – 260240Mast cell protease 1PRO_0000027440Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi50 ↔ 66PROSITE-ProRule annotation
    Disulfide bondi143 ↔ 208PROSITE-ProRule annotation
    Disulfide bondi174 ↔ 187PROSITE-ProRule annotation

    Keywords - PTMi

    Disulfide bond, Zymogen

    Proteomic databases

    PaxDbiP09650.
    PRIDEiP09650.

    Expressioni

    Gene expression databases

    GenevestigatoriP09650.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000028012.

    Structurei

    3D structure databases

    ProteinModelPortaliP09650.
    SMRiP09650. Positions 21-243.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini21 – 244224Peptidase S1PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the peptidase S1 family. Granzyme subfamily.PROSITE-ProRule annotation
    Contains 1 peptidase S1 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG5640.
    GeneTreeiENSGT00740000115279.
    HOGENOMiHOG000251820.
    HOVERGENiHBG013304.
    InParanoidiP09650.
    OMAiYMAQLEI.
    PhylomeDBiP09650.
    TreeFamiTF333630.

    Family and domain databases

    InterProiIPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view]
    PfamiPF00089. Trypsin. 1 hit.
    [Graphical view]
    PRINTSiPR00722. CHYMOTRYPSIN.
    SMARTiSM00020. Tryp_SPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF50494. SSF50494. 1 hit.
    PROSITEiPS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P09650-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQALLFLMAL LLPSGAGAEE IIGGVESRPH SRPYMAHLEI TTERGYKATC    50
    GGFLVTRQFV MTAAHCKGRE TTVTLGVHDV SKTESTQQKI KVEKQIVHPN 100
    YNFYSNLHDI MLLKLQKKAK VTPAVDVIPL PQPSDFLKPG KMCRAAGWGQ 150
    TGVTKPTSNT LREVKQRIMD KEACKNYFHY NYNFQVCVGS PRKIRSAYKG 200
    DSGGPLVCAG VAHGIVSYGR GDAKPPAVFT RISPYVPWIN KVIKGKDLTS 250
    LSLHESESPS 260
    Length:260
    Mass (Da):28,617
    Last modified:November 1, 1997 - v3
    Checksum:i076278089C1F8A96
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti56 – 583TRQ → NRN AA sequence (PubMed:3122823)Curated
    Sequence conflicti70 – 712ET → NE AA sequence (PubMed:3122823)Curated
    Sequence conflicti91 – 944KVEK → ALEI AA sequence (PubMed:8336143)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U67915 mRNA. Translation: AAB48268.1.
    S69206 mRNA. Translation: AAB30269.1.
    PIRiJC2125.
    RefSeqiNP_001264597.1. NM_001277668.1.
    UniGeneiRn.145977.
    Rn.202971.

    Genome annotation databases

    EnsembliENSRNOT00000028012; ENSRNOP00000028012; ENSRNOG00000020633.
    GeneIDi100360872.
    KEGGirno:100360872.
    UCSCiRGD:3062. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U67915 mRNA. Translation: AAB48268.1 .
    S69206 mRNA. Translation: AAB30269.1 .
    PIRi JC2125.
    RefSeqi NP_001264597.1. NM_001277668.1.
    UniGenei Rn.145977.
    Rn.202971.

    3D structure databases

    ProteinModelPortali P09650.
    SMRi P09650. Positions 21-243.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000028012.

    Protein family/group databases

    MEROPSi S01.149.

    Proteomic databases

    PaxDbi P09650.
    PRIDEi P09650.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000028012 ; ENSRNOP00000028012 ; ENSRNOG00000020633 .
    GeneIDi 100360872.
    KEGGi rno:100360872.
    UCSCi RGD:3062. rat.

    Organism-specific databases

    CTDi 100360872.
    RGDi 3062. Mcpt1.

    Phylogenomic databases

    eggNOGi COG5640.
    GeneTreei ENSGT00740000115279.
    HOGENOMi HOG000251820.
    HOVERGENi HBG013304.
    InParanoidi P09650.
    OMAi YMAQLEI.
    PhylomeDBi P09650.
    TreeFami TF333630.

    Miscellaneous databases

    PROi P09650.

    Gene expression databases

    Genevestigatori P09650.

    Family and domain databases

    InterProi IPR001254. Peptidase_S1.
    IPR018114. Peptidase_S1_AS.
    IPR001314. Peptidase_S1A.
    IPR009003. Trypsin-like_Pept_dom.
    [Graphical view ]
    Pfami PF00089. Trypsin. 1 hit.
    [Graphical view ]
    PRINTSi PR00722. CHYMOTRYPSIN.
    SMARTi SM00020. Tryp_SPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF50494. SSF50494. 1 hit.
    PROSITEi PS50240. TRYPSIN_DOM. 1 hit.
    PS00134. TRYPSIN_HIS. 1 hit.
    PS00135. TRYPSIN_SER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Secretory granule proteases in rat mast cells. Cloning of 10 different serine proteases and a carboxypeptidase A from various rat mast cell populations."
      Lutzelschwab C., Pejler G., Aveskogh M., Hellman L.
      J. Exp. Med. 185:13-29(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Mast cell.
    2. "Molecular cloning of rat mast cell protease 1 and development of specific probes for its gene transcript."
      Rouleau A., Garbarg M., Schwartz J.C., Ruat M.
      Biochem. Biophys. Res. Commun. 199:593-602(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 10-260.
    3. "Amino acid sequence of rat mast cell protease I (chymase)."
      le Trong H., Parmelee D.C., Walsh K.A., Neurath H., Woodbury R.G.
      Biochemistry 26:6988-6994(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 21-247.
    4. "A major serine protease in rat skeletal muscle: evidence for its mast cell origin."
      Woodbury R.G., Everitt M., Sanada Y., Katunuma N., Lagunoff D., Neurath H.
      Proc. Natl. Acad. Sci. U.S.A. 75:5311-5313(1978) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 21-73.
    5. "Identification of a chymotrypsin-like mast cell protease in rat brain capable of generating the N-terminus of the Alzheimer amyloid beta-protein."
      Nelson R.B., Siman R., Iqbal M.A., Potter H.
      J. Neurochem. 61:567-577(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 21-34; 91-101 AND 232-238, FUNCTION.

    Entry informationi

    Entry nameiMCPT1_RAT
    AccessioniPrimary (citable) accession number: P09650
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 123 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Peptidase families
      Classification of peptidase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3