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P09650 (MCPT1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 122. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Mast cell protease 1

Short name=rMCP-1
EC=3.4.21.39
Alternative name(s):
Chymase
Chymotrypsin-like protease
Short name=CLIP protein
Mast cell protease I
Short name=rMCP-I
Gene names
Name:Mcpt1
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length260 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion. May participate in generating perivascular beta-protein which ultimately aggregates into beta-amyloid deposits. Ref.5

Catalytic activity

Preferential cleavage: Phe-|-Xaa > Tyr-|-Xaa > Trp-|-Xaa > Leu-|-Xaa.

Subcellular location

Secreted By similarity. Cytoplasmic granule By similarity. Note: Mast cell granules By similarity.

Sequence similarities

Belongs to the peptidase S1 family. Granzyme subfamily.

Contains 1 peptidase S1 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1818 Potential
Propeptide19 – 202Activation peptide
PRO_0000027439
Chain21 – 260240Mast cell protease 1
PRO_0000027440

Regions

Domain21 – 244224Peptidase S1

Sites

Active site651Charge relay system By similarity
Active site1091Charge relay system By similarity
Active site2021Charge relay system By similarity

Amino acid modifications

Disulfide bond50 ↔ 66 By similarity
Disulfide bond143 ↔ 208 By similarity
Disulfide bond174 ↔ 187 By similarity

Experimental info

Sequence conflict56 – 583TRQ → NRN AA sequence Ref.3
Sequence conflict70 – 712ET → NE AA sequence Ref.3
Sequence conflict91 – 944KVEK → ALEI AA sequence Ref.5

Sequences

Sequence LengthMass (Da)Tools
P09650 [UniParc].

Last modified November 1, 1997. Version 3.
Checksum: 076278089C1F8A96

FASTA26028,617
        10         20         30         40         50         60 
MQALLFLMAL LLPSGAGAEE IIGGVESRPH SRPYMAHLEI TTERGYKATC GGFLVTRQFV 

        70         80         90        100        110        120 
MTAAHCKGRE TTVTLGVHDV SKTESTQQKI KVEKQIVHPN YNFYSNLHDI MLLKLQKKAK 

       130        140        150        160        170        180 
VTPAVDVIPL PQPSDFLKPG KMCRAAGWGQ TGVTKPTSNT LREVKQRIMD KEACKNYFHY 

       190        200        210        220        230        240 
NYNFQVCVGS PRKIRSAYKG DSGGPLVCAG VAHGIVSYGR GDAKPPAVFT RISPYVPWIN 

       250        260 
KVIKGKDLTS LSLHESESPS 

« Hide

References

[1]"Secretory granule proteases in rat mast cells. Cloning of 10 different serine proteases and a carboxypeptidase A from various rat mast cell populations."
Lutzelschwab C., Pejler G., Aveskogh M., Hellman L.
J. Exp. Med. 185:13-29(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Mast cell.
[2]"Molecular cloning of rat mast cell protease 1 and development of specific probes for its gene transcript."
Rouleau A., Garbarg M., Schwartz J.C., Ruat M.
Biochem. Biophys. Res. Commun. 199:593-602(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 10-260.
[3]"Amino acid sequence of rat mast cell protease I (chymase)."
le Trong H., Parmelee D.C., Walsh K.A., Neurath H., Woodbury R.G.
Biochemistry 26:6988-6994(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 21-247.
[4]"A major serine protease in rat skeletal muscle: evidence for its mast cell origin."
Woodbury R.G., Everitt M., Sanada Y., Katunuma N., Lagunoff D., Neurath H.
Proc. Natl. Acad. Sci. U.S.A. 75:5311-5313(1978) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 21-73.
[5]"Identification of a chymotrypsin-like mast cell protease in rat brain capable of generating the N-terminus of the Alzheimer amyloid beta-protein."
Nelson R.B., Siman R., Iqbal M.A., Potter H.
J. Neurochem. 61:567-577(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 21-34; 91-101 AND 232-238, FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U67915 mRNA. Translation: AAB48268.1.
S69206 mRNA. Translation: AAB30269.1.
PIRJC2125.
RefSeqNP_001264597.1. NM_001277668.1.
UniGeneRn.145977.
Rn.202971.

3D structure databases

ProteinModelPortalP09650.
SMRP09650. Positions 21-243.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000028012.

Protein family/group databases

MEROPSS01.149.

Proteomic databases

PaxDbP09650.
PRIDEP09650.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000028012; ENSRNOP00000028012; ENSRNOG00000020633.
GeneID100360872.
KEGGrno:100360872.
UCSCRGD:3062. rat.

Organism-specific databases

CTD100360872.
RGD3062. Mcpt1.

Phylogenomic databases

eggNOGCOG5640.
GeneTreeENSGT00740000115279.
HOGENOMHOG000251820.
HOVERGENHBG013304.
InParanoidP09650.
OMAYMAQLEI.
PhylomeDBP09650.
TreeFamTF333630.

Gene expression databases

GenevestigatorP09650.

Family and domain databases

InterProIPR001254. Peptidase_S1.
IPR018114. Peptidase_S1_AS.
IPR001314. Peptidase_S1A.
IPR009003. Trypsin-like_Pept_dom.
[Graphical view]
PfamPF00089. Trypsin. 1 hit.
[Graphical view]
PRINTSPR00722. CHYMOTRYPSIN.
SMARTSM00020. Tryp_SPc. 1 hit.
[Graphical view]
SUPFAMSSF50494. SSF50494. 1 hit.
PROSITEPS50240. TRYPSIN_DOM. 1 hit.
PS00134. TRYPSIN_HIS. 1 hit.
PS00135. TRYPSIN_SER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP09650.

Entry information

Entry nameMCPT1_RAT
AccessionPrimary (citable) accession number: P09650
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: November 1, 1997
Last modified: April 16, 2014
This is version 122 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries