Reviewed, UniProtKB/Swiss-Prot P09648 (CATL1_CHICK)

Names and origin

Protein names

Recommended name:
    Cathepsin L1
    EC=3.4.22.15
Cleaved into the following 2 chains:
    1- Recommended name:
            Cathepsin L1 heavy chain
    2- Recommended name:
            Cathepsin L1 light chain

Gene names

Name:	CTSL1
Synonyms:	CTSL

Organism

Gallus gallus (Chicken)

Taxonomic identifier

9031 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length	218 AA.
Sequence status	Fragments.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Important for the overall degradation of proteins in lysosomes.
Catalytic activity	Specificity close to that of papain. As compared to cathepsin B, cathepsin L exhibits higher activity toward protein substrates, but has little activity on Z-Arg-Arg-NHMec, and no peptidyl-dipeptidase activity.
Subunit structure	Dimer of a heavy and a light chain linked by disulfide bonds.
Subcellular location	Lysosome.
Sequence similarities	Belongs to the peptidase C1 family.

Ontologies

Keywords
Cellular component	Lysosome
Molecular function	Hydrolase Protease Thiol protease
PTM	Disulfide bond Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 176	176	Cathepsin L1 heavy chain		PRO_0000026263
	Chain	177 – 218	42	Cathepsin L1 light chain		PRO_0000026264
Sites
	Active site	25	1	By similarity
	Active site	165	1	By similarity
	Active site	185	1	By similarity
Amino acid modifications
	Glycosylation	109	1	N-linked (GlcNAc...)
	Disulfide bond	22 ↔ 65		By similarity
	Disulfide bond	56 ↔ 99		By similarity
	Disulfide bond	158 ↔ 207		Interchain (between heavy and light chains) By similarity
Experimental info
	Sequence conflict	21	1	Q → I AA sequence Ref.3
	Sequence conflict	29	1	S → N AA sequence Ref.3
	Sequence conflict	40 – 42	3	RTK → FKT AA sequence Ref.3
	Sequence conflict	97 – 98	2	Missing AA sequence Ref.3
	Sequence conflict	109	1	N → K AA sequence Ref.3
	Sequence conflict	177	1	Missing AA sequence Ref.3
	Sequence conflict	197	1	I → Q AA sequence Ref.3
	Non-adjacent residues	176 – 177	2

Sequences

	Sequence		Length	Mass (Da)
	P09648-1 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: D26E21BC071686C2	FASTA	218	23,963
	10 20 30 40 50 60 APRSVDWREK GYVTPVKDQG QCGSCWAFST TGALEGQHFR TKGKLVSLSE QNLVDCSRPE 70 80 90 100 110 120 GNQGCNGGLM DQAFQYVQDN GGIDSEESYP YTAKDDEDCR YKAEYNAAND TGFVDIPQGH 130 140 150 160 170 180 ERALMKAVAS VGPVSVAIDA GHSSFQFYQS GIYYEPDCSS EDLDHGVLVV GYGFEGGKKY 190 200 210 WIVKNSWGEK WGDKGYIYMA KDRKNHCGIA TAASYPLV

References

[1]	"Amino acid sequence of chicken liver cathepsin L." Wada K., Takai T., Tanabe T. Eur. J. Biochem. 167:13-18(1987) [PubMed: 3305012] [Abstract] Cited for: PROTEIN SEQUENCE.
[2]	"N-terminal amino acid sequences of the heavy and light chains of chicken liver cathepsin L." Wada K., Tanabe T. FEBS Lett. 209:330-334(1986) [PubMed: 3792553] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-37 AND 177-216. Tissue: Liver.
[3]	"Purification and amino acid sequence of chicken liver cathepsin L." Dufour E., Obled A., Valin C., Bechet D., Ribadeau-Dumas B., Huet J.-C. Biochemistry 26:5689-5695(1987) [PubMed: 3676277] [Abstract] Cited for: PROTEIN SEQUENCE. Tissue: Liver.

Cross-references

Sequence databases
IPI	IPI00602255.
PIR	KHCHL. S00081.
UniGene	Gga.523
3D structure databases
SMR	P09648. Positions 1-218.
ModBase	Search...
Protein-protein interaction databases
STRING	P09648.
Protein family/group databases
MEROPS	C01.032.
Genome annotation databases
Ensembl	ENSGALT00000037988; ENSGALP00000037193; ENSGALG00000012610; Gallus gallus. [Genome view]
Phylogenomic databases
eggNOG	veNOG04454.
HOVERGEN	P09648.
Enzyme and pathway databases
BRENDA	3.4.22.15. 4.
Family and domain databases
InterPro	IPR000169. Pept_cys_AS. IPR013128. Peptidase_C1A. IPR000668. Peptidase_C1A_C. [Graphical view]
PANTHER	PTHR12411. Peptidase_C1A. 1 hit.
Pfam	PF00112. Peptidase_C1. 1 hit. [Graphical view]
PRINTS	PR00705. PAPAIN.
SMART	SM00645. Pept_C1. 1 hit. [Graphical view]
PROSITE	PS00640. THIOL_PROTEASE_ASN. 1 hit. PS00139. THIOL_PROTEASE_CYS. 1 hit. PS00639. THIOL_PROTEASE_HIS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CATL1_CHICK

Accession

Primary (citable) accession number: P09648

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	January 19, 2010
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)