P09647 (RNPC_PENCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Guanyl-specific ribonuclease Pc Short name=RNase Pc EC=3.1.27.3
Organism	Penicillium chrysogenum (Penicillium notatum)
Taxonomic identifier	5076 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › Eurotiomycetes › Eurotiomycetidae › Eurotiales › Trichocomaceae › mitosporic Trichocomaceae › Penicillium › Penicillium chrysogenum complex

Protein attributes

Sequence length	102 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in G-P with 2',3'-cyclic phosphate intermediates.
Sequence similarities	Belongs to the ribonuclease N1/T1 family.

Ontologies

Keywords
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Disulfide bond
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro endoribonuclease activity Inferred from electronic annotation. Source: InterPro ribonuclease T1 activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 102	102	Guanyl-specific ribonuclease Pc		PRO_0000137373
Sites
	Active site	38	1	By similarity
	Active site	56	1	Proton acceptor By similarity
	Active site	90	1	Proton donor By similarity
Amino acid modifications
	Disulfide bond	2 ↔ 10		By similarity
	Disulfide bond	6 ↔ 101		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P09647 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 49D8947B267D5506	FASTA	102	10,745
	10 20 30 40 50 60 ACAATCGSVC YTSSAISAAQ EAGYDLYSAN DDVSNYPHEY RNYEGFDFPV SGTYYEFPIL 70 80 90 100 RSGAVYSGNS PGADRVVFNG NDQLAGVITH TGASGNNFVA CD

References

[1]	"Express analysis of protein amino acid sequences. Primary structure of Penicillium chrysogenum 152A guanyl-specific ribonuclease." Shlyapnikov S.V., Bezborodova S.I., Kulikov V.A., Yakovlev G.I. FEBS Lett. 196:29-33(1986) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE. Strain: 152A.

Cross-references

Sequence databases
PIR	A23620.
3D structure databases
ProteinModelPortal	P09647.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.10.450.30. 1 hit.
InterPro	IPR000026. Gua-sp_ribonuclease_N1/T1. IPR016191. Ribonuclease/ribotoxin. [Graphical view]
Pfam	PF00545. Ribonuclease. 1 hit. [Graphical view]
SUPFAM	SSF53933. Ribonuclease/ribotoxin. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RNPC_PENCH

Accession

Primary (citable) accession number: P09647

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	April 3, 2013
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program