ID   RNN1_NEUCR              Reviewed;         153 AA.
AC   P09646; Q1K8C1; Q8WZP7; V5IL75;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   31-AUG-2004, sequence version 2.
DT   27-MAR-2024, entry version 126.
DE   RecName: Full=Guanyl-specific ribonuclease N1;
DE            Short=RNase N1;
DE            EC=4.6.1.24;
DE   Flags: Precursor;
GN   Name=grn; ORFNames=G15G9.140, NCU01045;
OS   Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 /
OS   FGSC 987).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes;
OC   Sordariomycetidae; Sordariales; Sordariaceae; Neurospora.
OX   NCBI_TaxID=367110;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=12655011; DOI=10.1093/nar/gkg293;
RA   Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D.,
RA   Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.;
RT   "What's in the genome of a filamentous fungus? Analysis of the Neurospora
RT   genome sequence.";
RL   Nucleic Acids Res. 31:1944-1954(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987;
RX   PubMed=12712197; DOI=10.1038/nature01554;
RA   Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D.,
RA   Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B.,
RA   Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M.,
RA   Qui D., Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M.,
RA   Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U.,
RA   Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D.,
RA   Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S.,
RA   Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D.,
RA   Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S.,
RA   Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A.,
RA   DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R.,
RA   Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R.,
RA   Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I.,
RA   Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.;
RT   "The genome sequence of the filamentous fungus Neurospora crassa.";
RL   Nature 422:859-868(2003).
RN   [3]
RP   PROTEIN SEQUENCE OF 50-153.
RX   PubMed=2977130; DOI=10.1093/oxfordjournals.jbchem.a122476;
RA   Takahashi K.;
RT   "The amino acid sequence of ribonuclease N1, a guanine-specific
RT   ribonuclease from the fungus Neurospora crassa.";
RL   J. Biochem. 104:375-382(1988).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=[RNA] containing guanosine + H2O = an [RNA fragment]-3'-
CC         guanosine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA
CC         fragment].; EC=4.6.1.24;
CC   -!- SIMILARITY: Belongs to the ribonuclease N1/T1 family. {ECO:0000305}.
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DR   EMBL; AL670006; CAD21301.1; -; Genomic_DNA.
DR   EMBL; CM002240; ESA42488.1; -; Genomic_DNA.
DR   PIR; JT0301; NRNCT1.
DR   RefSeq; XP_011394457.1; XM_011396155.1.
DR   AlphaFoldDB; P09646; -.
DR   SMR; P09646; -.
DR   STRING; 367110.P09646; -.
DR   PaxDb; 5141-EFNCRP00000004227; -.
DR   EnsemblFungi; ESA42488; ESA42488; NCU01045.
DR   GeneID; 3877848; -.
DR   KEGG; ncr:NCU01045; -.
DR   VEuPathDB; FungiDB:NCU01045; -.
DR   HOGENOM; CLU_111658_1_0_1; -.
DR   InParanoid; P09646; -.
DR   OMA; ACAYTCG; -.
DR   OrthoDB; 1698814at2759; -.
DR   BRENDA; 4.6.1.24; 3627.
DR   Proteomes; UP000001805; Chromosome 2, Linkage Group V.
DR   GO; GO:0004519; F:endonuclease activity; IEA:UniProtKB-KW.
DR   GO; GO:0016829; F:lyase activity; IEA:UniProtKB-KW.
DR   GO; GO:0046589; F:ribonuclease T1 activity; IEA:UniProtKB-EC.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   CDD; cd00606; fungal_RNase; 1.
DR   Gene3D; 3.10.450.30; Microbial ribonucleases; 1.
DR   InterPro; IPR016191; Ribonuclease/ribotoxin.
DR   InterPro; IPR048269; RNase_U2.
DR   PANTHER; PTHR42104; EXTRACELLULAR GUANYL-SPECIFIC RIBONUCLEASE RNTA (AFU_ORTHOLOGUE AFUA_4G03230); 1.
DR   PANTHER; PTHR42104:SF1; EXTRACELLULAR GUANYL-SPECIFIC RIBONUCLEASE RNTA (AFU_ORTHOLOGUE AFUA_4G03230); 1.
DR   PIRSF; PIRSF037430; RNase_U2; 1.
DR   SUPFAM; SSF53933; Microbial ribonucleases; 1.
PE   1: Evidence at protein level;
KW   Direct protein sequencing; Disulfide bond; Endonuclease; Hydrolase; Lyase;
KW   Nuclease; Reference proteome; Signal.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   PROPEP          21..49
FT                   /evidence="ECO:0000269|PubMed:2977130"
FT                   /id="PRO_0000030831"
FT   CHAIN           50..153
FT                   /note="Guanyl-specific ribonuclease N1"
FT                   /id="PRO_0000030832"
FT   ACT_SITE        89
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        107
FT                   /note="Proton acceptor"
FT                   /evidence="ECO:0000250"
FT   ACT_SITE        141
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250"
FT   DISULFID        51..59
FT                   /evidence="ECO:0000269|PubMed:2977130"
FT   DISULFID        55..152
FT                   /evidence="ECO:0000269|PubMed:2977130"
SQ   SEQUENCE   153 AA;  16012 MW;  2F00E46B7ED45A39 CRC64;
     MVQLLSAFVS LLSVVAVSGA AIPAPAPEAV VDVAPETATI EPTGNFTAQA CMYICGSVCY
     SSSAISAALN KGYSYYEDGA TAGSSSYPHR YNNYEGFDFP TAKPWYEFPI LSSGRVYTGG
     SPGADRVIFD SHGNLDMLIT HNGASGNNFV ACN
//