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P09646

- RNN1_NEUCR

UniProt

P09646 - RNN1_NEUCR

Protein

Guanyl-specific ribonuclease N1

Gene

grn

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 2 (31 Aug 2004)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in G-P with 2',3'-cyclic phosphate intermediates.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei89 – 891By similarity
    Active sitei107 – 1071Proton acceptorBy similarity
    Active sitei141 – 1411Proton donorBy similarity

    GO - Molecular functioni

    1. endoribonuclease activity Source: InterPro
    2. ribonuclease T1 activity Source: UniProtKB-EC
    3. RNA binding Source: InterPro

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Guanyl-specific ribonuclease N1 (EC:3.1.27.3)
    Short name:
    RNase N1
    Gene namesi
    Name:grn
    ORF Names:G15G9.140, NCU01045
    OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
    Taxonomic identifieri367110 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
    ProteomesiUP000001805: Chromosome 2, Linkage Group V

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2020Sequence AnalysisAdd
    BLAST
    Propeptidei21 – 49291 PublicationPRO_0000030831Add
    BLAST
    Chaini50 – 153104Guanyl-specific ribonuclease N1PRO_0000030832Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi51 ↔ 591 Publication
    Disulfide bondi55 ↔ 1521 Publication

    Keywords - PTMi

    Disulfide bond

    Structurei

    3D structure databases

    ProteinModelPortaliP09646.
    SMRiP09646. Positions 50-152.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ribonuclease N1/T1 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG114265.
    HOGENOMiHOG000217295.
    OMAiYQEFPIR.
    OrthoDBiEOG7XSTSF.

    Family and domain databases

    Gene3Di3.10.450.30. 1 hit.
    InterProiIPR000026. Gua-sp_ribonuclease_N1/T1.
    IPR016191. Ribonuclease/ribotoxin.
    [Graphical view]
    PfamiPF00545. Ribonuclease. 1 hit.
    [Graphical view]
    SUPFAMiSSF53933. SSF53933. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P09646-1 [UniParc]FASTAAdd to Basket

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    MVQLLSAFVS LLSVVAVSGA AIPAPAPEAV VDVAPETATI EPTGNFTAQA    50
    CMYICGSVCY SSSAISAALN KGYSYYEDGA TAGSSSYPHR YNNYEGFDFP 100
    TAKPWYEFPI LSSGRVYTGG SPGADRVIFD SHGNLDMLIT HNGASGNNFV 150
    ACN 153
    Length:153
    Mass (Da):16,012
    Last modified:August 31, 2004 - v2
    Checksum:i2F00E46B7ED45A39
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL670006 Genomic DNA. Translation: CAD21301.1.
    CM002240 Genomic DNA. Translation: ESA42488.1.
    PIRiJT0301. NRNCT1.
    RefSeqiXP_961657.1. XM_956564.1.

    Genome annotation databases

    EnsemblFungiiEFNCRT00000004227; EFNCRP00000004227; EFNCRG00000004222.
    GeneIDi3877848.
    KEGGincr:NCU01045.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AL670006 Genomic DNA. Translation: CAD21301.1 .
    CM002240 Genomic DNA. Translation: ESA42488.1 .
    PIRi JT0301. NRNCT1.
    RefSeqi XP_961657.1. XM_956564.1.

    3D structure databases

    ProteinModelPortali P09646.
    SMRi P09646. Positions 50-152.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii EFNCRT00000004227 ; EFNCRP00000004227 ; EFNCRG00000004222 .
    GeneIDi 3877848.
    KEGGi ncr:NCU01045.

    Phylogenomic databases

    eggNOGi NOG114265.
    HOGENOMi HOG000217295.
    OMAi YQEFPIR.
    OrthoDBi EOG7XSTSF.

    Family and domain databases

    Gene3Di 3.10.450.30. 1 hit.
    InterProi IPR000026. Gua-sp_ribonuclease_N1/T1.
    IPR016191. Ribonuclease/ribotoxin.
    [Graphical view ]
    Pfami PF00545. Ribonuclease. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53933. SSF53933. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence."
      Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.
      Nucleic Acids Res. 31:1944-1954(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
    2. "The genome sequence of the filamentous fungus Neurospora crassa."
      Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
      , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
      Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
    3. "The amino acid sequence of ribonuclease N1, a guanine-specific ribonuclease from the fungus Neurospora crassa."
      Takahashi K.
      J. Biochem. 104:375-382(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 50-153.

    Entry informationi

    Entry nameiRNN1_NEUCR
    AccessioniPrimary (citable) accession number: P09646
    Secondary accession number(s): Q1K8C1, Q8WZP7, V5IL75
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: August 31, 2004
    Last modified: October 1, 2014
    This is version 87 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3