P09646 (RNN1_NEUCR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Guanyl-specific ribonuclease N1
Short name=RNase N1
EC=3.1.27.3

Gene names

ORF Names:

G15G9.140, NCU01045

Organism

Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) [Reference proteome]

Taxonomic identifier

367110 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Pezizomycotina › leotiomyceta › sordariomyceta › Sordariomycetes › Sordariomycetidae › Sordariales › Sordariaceae › Neurospora › Neurospora crassa

Protein attributes

Sequence length	153 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in G-P with 2',3'-cyclic phosphate intermediates.
Sequence similarities	Belongs to the ribonuclease N1/T1 family.

Ontologies

Keywords
Domain	Signal
Molecular function	Endonuclease Hydrolase Nuclease
PTM	Disulfide bond
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro endoribonuclease activity Inferred from electronic annotation. Source: InterPro ribonuclease T1 activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Propeptide	21 – 49	29			PRO_0000030831
	Chain	50 – 153	104	Guanyl-specific ribonuclease N1		PRO_0000030832
Sites
	Active site	89	1	By similarity
	Active site	107	1	Proton acceptor By similarity
	Active site	141	1	Proton donor By similarity
Amino acid modifications
	Disulfide bond	51 ↔ 59		Ref.3
	Disulfide bond	55 ↔ 152		Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P09646 [UniParc]. Last modified August 31, 2004. Version 2. Checksum: 2F00E46B7ED45A39	FASTA	153	16,012
	10 20 30 40 50 60 MVQLLSAFVS LLSVVAVSGA AIPAPAPEAV VDVAPETATI EPTGNFTAQA CMYICGSVCY 70 80 90 100 110 120 SSSAISAALN KGYSYYEDGA TAGSSSYPHR YNNYEGFDFP TAKPWYEFPI LSSGRVYTGG 130 140 150 SPGADRVIFD SHGNLDMLIT HNGASGNNFV ACN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence." Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U. Nucleic Acids Res. 31:1944-1954(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[2]	"The genome sequence of the filamentous fungus Neurospora crassa." Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W. Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[3]	"The amino acid sequence of ribonuclease N1, a guanine-specific ribonuclease from the fungus Neurospora crassa." Takahashi K. J. Biochem. 104:375-382(1988) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 50-153.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AL670006 Genomic DNA. Translation: CAD21301.1. AABX02000015 Genomic DNA. Translation: EAA32421.1.
PIR	NRNCT1. JT0301.
RefSeq	XP_961657.1. XM_956564.1.
3D structure databases
ProteinModelPortal	P09646.
SMR	P09646. Positions 50-152.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	EFNCRT00000004227; EFNCRP00000004227; EFNCRG00000004222.
GeneID	3877848.
KEGG	ncr:NCU01045.
Phylogenomic databases
eggNOG	NOG114265.
HOGENOM	HOG000217295.
OMA	NNYEGFD.
OrthoDB	EOG4GBBGV.
Family and domain databases
Gene3D	3.10.450.30. 1 hit.
InterPro	IPR000026. Gua-sp_ribonuclease_N1/T1. IPR016191. Ribonuclease/ribotoxin. [Graphical view]
Pfam	PF00545. Ribonuclease. 1 hit. [Graphical view]
SUPFAM	SSF53933. Ribonuclease/ribotoxin. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RNN1_NEUCR

Accession

Primary (citable) accession number: P09646
Secondary accession number(s): Q1K8C1, Q8WZP7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	August 31, 2004
Last modified:	April 3, 2013
This is version 80 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program