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P09646

- RNN1_NEUCR

UniProt

P09646 - RNN1_NEUCR

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Protein

Guanyl-specific ribonuclease N1

Gene

grn

Organism
Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

Two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in G-P with 2',3'-cyclic phosphate intermediates.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei89 – 891By similarity
Active sitei107 – 1071Proton acceptorBy similarity
Active sitei141 – 1411Proton donorBy similarity

GO - Molecular functioni

  1. endoribonuclease activity Source: InterPro
  2. ribonuclease T1 activity Source: UniProtKB-EC
  3. RNA binding Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Names & Taxonomyi

Protein namesi
Recommended name:
Guanyl-specific ribonuclease N1 (EC:3.1.27.3)
Short name:
RNase N1
Gene namesi
Name:grn
ORF Names:G15G9.140, NCU01045
OrganismiNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Taxonomic identifieri367110 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora
ProteomesiUP000001805: Chromosome 2, Linkage Group V

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020Sequence AnalysisAdd
BLAST
Propeptidei21 – 49291 PublicationPRO_0000030831Add
BLAST
Chaini50 – 153104Guanyl-specific ribonuclease N1PRO_0000030832Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi51 ↔ 591 Publication
Disulfide bondi55 ↔ 1521 Publication

Keywords - PTMi

Disulfide bond

Structurei

3D structure databases

ProteinModelPortaliP09646.
SMRiP09646. Positions 50-152.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ribonuclease N1/T1 family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG114265.
HOGENOMiHOG000217295.
InParanoidiP09646.
OMAiYQEFPIR.
OrthoDBiEOG7XSTSF.

Family and domain databases

Gene3Di3.10.450.30. 1 hit.
InterProiIPR000026. Gua-sp_ribonuclease_N1/T1.
IPR016191. Ribonuclease/ribotoxin.
[Graphical view]
PfamiPF00545. Ribonuclease. 1 hit.
[Graphical view]
SUPFAMiSSF53933. SSF53933. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09646-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MVQLLSAFVS LLSVVAVSGA AIPAPAPEAV VDVAPETATI EPTGNFTAQA
60 70 80 90 100
CMYICGSVCY SSSAISAALN KGYSYYEDGA TAGSSSYPHR YNNYEGFDFP
110 120 130 140 150
TAKPWYEFPI LSSGRVYTGG SPGADRVIFD SHGNLDMLIT HNGASGNNFV

ACN
Length:153
Mass (Da):16,012
Last modified:August 31, 2004 - v2
Checksum:i2F00E46B7ED45A39
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL670006 Genomic DNA. Translation: CAD21301.1.
CM002240 Genomic DNA. Translation: ESA42488.1.
PIRiJT0301. NRNCT1.
RefSeqiXP_961657.1. XM_956564.1.

Genome annotation databases

EnsemblFungiiEFNCRT00000004227; EFNCRP00000004227; EFNCRG00000004222.
GeneIDi3877848.
KEGGincr:NCU01045.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL670006 Genomic DNA. Translation: CAD21301.1 .
CM002240 Genomic DNA. Translation: ESA42488.1 .
PIRi JT0301. NRNCT1.
RefSeqi XP_961657.1. XM_956564.1.

3D structure databases

ProteinModelPortali P09646.
SMRi P09646. Positions 50-152.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii EFNCRT00000004227 ; EFNCRP00000004227 ; EFNCRG00000004222 .
GeneIDi 3877848.
KEGGi ncr:NCU01045.

Phylogenomic databases

eggNOGi NOG114265.
HOGENOMi HOG000217295.
InParanoidi P09646.
OMAi YQEFPIR.
OrthoDBi EOG7XSTSF.

Family and domain databases

Gene3Di 3.10.450.30. 1 hit.
InterProi IPR000026. Gua-sp_ribonuclease_N1/T1.
IPR016191. Ribonuclease/ribotoxin.
[Graphical view ]
Pfami PF00545. Ribonuclease. 1 hit.
[Graphical view ]
SUPFAMi SSF53933. SSF53933. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence."
    Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.
    Nucleic Acids Res. 31:1944-1954(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
  2. "The genome sequence of the filamentous fungus Neurospora crassa."
    Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D.
    , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
    Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
  3. "The amino acid sequence of ribonuclease N1, a guanine-specific ribonuclease from the fungus Neurospora crassa."
    Takahashi K.
    J. Biochem. 104:375-382(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 50-153.

Entry informationi

Entry nameiRNN1_NEUCR
AccessioniPrimary (citable) accession number: P09646
Secondary accession number(s): Q1K8C1, Q8WZP7, V5IL75
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: August 31, 2004
Last modified: October 29, 2014
This is version 88 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3