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P09646 (RNN1_NEUCR) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 86. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Guanyl-specific ribonuclease N1

Short name=RNase N1
EC=3.1.27.3
Gene names
Name:grn
ORF Names:G15G9.140, NCU01045
OrganismNeurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) [Reference proteome]
Taxonomic identifier367110 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaPezizomycotinaSordariomycetesSordariomycetidaeSordarialesSordariaceaeNeurospora

Protein attributes

Sequence length153 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

Two-stage endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in G-P with 2',3'-cyclic phosphate intermediates.

Sequence similarities

Belongs to the ribonuclease N1/T1 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 Potential
Propeptide21 – 4929
PRO_0000030831
Chain50 – 153104Guanyl-specific ribonuclease N1
PRO_0000030832

Sites

Active site891 By similarity
Active site1071Proton acceptor By similarity
Active site1411Proton donor By similarity

Amino acid modifications

Disulfide bond51 ↔ 59 Ref.3
Disulfide bond55 ↔ 152 Ref.3

Sequences

Sequence LengthMass (Da)Tools
P09646 [UniParc].

Last modified August 31, 2004. Version 2.
Checksum: 2F00E46B7ED45A39

FASTA15316,012
        10         20         30         40         50         60 
MVQLLSAFVS LLSVVAVSGA AIPAPAPEAV VDVAPETATI EPTGNFTAQA CMYICGSVCY 

        70         80         90        100        110        120 
SSSAISAALN KGYSYYEDGA TAGSSSYPHR YNNYEGFDFP TAKPWYEFPI LSSGRVYTGG 

       130        140        150 
SPGADRVIFD SHGNLDMLIT HNGASGNNFV ACN 

« Hide

References

« Hide 'large scale' references
[1]"What's in the genome of a filamentous fungus? Analysis of the Neurospora genome sequence."
Mannhaupt G., Montrone C., Haase D., Mewes H.-W., Aign V., Hoheisel J.D., Fartmann B., Nyakatura G., Kempken F., Maier J., Schulte U.
Nucleic Acids Res. 31:1944-1954(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[2]"The genome sequence of the filamentous fungus Neurospora crassa."
Galagan J.E., Calvo S.E., Borkovich K.A., Selker E.U., Read N.D., Jaffe D.B., FitzHugh W., Ma L.-J., Smirnov S., Purcell S., Rehman B., Elkins T., Engels R., Wang S., Nielsen C.B., Butler J., Endrizzi M., Qui D. expand/collapse author list , Ianakiev P., Bell-Pedersen D., Nelson M.A., Werner-Washburne M., Selitrennikoff C.P., Kinsey J.A., Braun E.L., Zelter A., Schulte U., Kothe G.O., Jedd G., Mewes H.-W., Staben C., Marcotte E., Greenberg D., Roy A., Foley K., Naylor J., Stange-Thomann N., Barrett R., Gnerre S., Kamal M., Kamvysselis M., Mauceli E.W., Bielke C., Rudd S., Frishman D., Krystofova S., Rasmussen C., Metzenberg R.L., Perkins D.D., Kroken S., Cogoni C., Macino G., Catcheside D.E.A., Li W., Pratt R.J., Osmani S.A., DeSouza C.P.C., Glass N.L., Orbach M.J., Berglund J.A., Voelker R., Yarden O., Plamann M., Seiler S., Dunlap J.C., Radford A., Aramayo R., Natvig D.O., Alex L.A., Mannhaupt G., Ebbole D.J., Freitag M., Paulsen I., Sachs M.S., Lander E.S., Nusbaum C., Birren B.W.
Nature 422:859-868(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987.
[3]"The amino acid sequence of ribonuclease N1, a guanine-specific ribonuclease from the fungus Neurospora crassa."
Takahashi K.
J. Biochem. 104:375-382(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 50-153.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AL670006 Genomic DNA. Translation: CAD21301.1.
CM002240 Genomic DNA. Translation: ESA42488.1.
PIRNRNCT1. JT0301.
RefSeqXP_961657.1. XM_956564.1.

3D structure databases

ProteinModelPortalP09646.
SMRP09646. Positions 50-152.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiEFNCRT00000004227; EFNCRP00000004227; EFNCRG00000004222.
GeneID3877848.
KEGGncr:NCU01045.

Phylogenomic databases

eggNOGNOG114265.
HOGENOMHOG000217295.
OMAYQEFPIR.
OrthoDBEOG7XSTSF.

Family and domain databases

Gene3D3.10.450.30. 1 hit.
InterProIPR000026. Gua-sp_ribonuclease_N1/T1.
IPR016191. Ribonuclease/ribotoxin.
[Graphical view]
PfamPF00545. Ribonuclease. 1 hit.
[Graphical view]
SUPFAMSSF53933. SSF53933. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRNN1_NEUCR
AccessionPrimary (citable) accession number: P09646
Secondary accession number(s): Q1K8C1, Q8WZP7, V5IL75
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: August 31, 2004
Last modified: April 16, 2014
This is version 86 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families