ID   HXB8_MOUSE              Reviewed;         243 AA.
AC   P09632;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-JAN-1990, sequence version 2.
DT   24-JAN-2024, entry version 182.
DE   RecName: Full=Homeobox protein Hox-B8;
DE   AltName: Full=Homeobox protein Hox-2.4;
GN   Name=Hoxb8; Synonyms=Hox-2.4, Hoxb-8;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=BALB/cJ;
RX   PubMed=2564662; DOI=10.1093/nar/17.5.1881;
RA   Kongsuan K., Allen J., Adams J.M.;
RT   "Expression of Hox-2.4 homeobox gene directed by proviral insertion in a
RT   myeloid leukemia.";
RL   Nucleic Acids Res. 17:1881-1892(1989).
RN   [2]
RP   NUCLEOTIDE SEQUENCE.
RC   STRAIN=BALB/cJ;
RX   PubMed=2907477; DOI=10.1002/j.1460-2075.1988.tb03326.x;
RA   Blatt C., Aberdam D., Schwartz R., Sachs L.;
RT   "DNA rearrangement of a homeobox gene in myeloid leukaemic cells.";
RL   EMBO J. 7:4283-4290(1988).
RN   [3]
RP   SEQUENCE REVISION.
RA   Blatt C.;
RL   Submitted (JUL-1989) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1673811; DOI=10.1016/0042-6822(91)90686-6;
RA   Ben-David L., Aberdam D., Sachs L., Blatt C.;
RT   "A deletion and a rearrangement distinguish between the intracisternal
RT   A- particle of Hox-2.4 and that of interleukin-3 in the same leukemic
RT   cells.";
RL   Virology 182:382-387(1991).
RN   [5]
RP   NUCLEOTIDE SEQUENCE OF 144-243.
RX   PubMed=2891608; DOI=10.1016/0888-7543(87)90011-5;
RA   Hart C.P., Fainsod A., Ruddle F.H.;
RT   "Sequence analysis of the murine Hox-2.2, -2.3, and -2.4 homeo boxes:
RT   evolutionary and structural comparisons.";
RL   Genomics 1:182-195(1987).
RN   [6]
RP   INTERACTION WITH PBX1.
RX   PubMed=7791786; DOI=10.1128/mcb.15.7.3786;
RA   Lu Q., Knoepfler P.S., Scheele J., Wright D.D., Kamps M.P.;
RT   "Both Pbx1 and E2A-Pbx1 bind the DNA motif ATCAATCAA cooperatively with the
RT   products of multiple murine Hox genes, some of which are themselves
RT   oncogenes.";
RL   Mol. Cell. Biol. 15:3786-3795(1995).
CC   -!- FUNCTION: Sequence-specific transcription factor which is part of a
CC       developmental regulatory system that provides cells with specific
CC       positional identities on the anterior-posterior axis.
CC   -!- SUBUNIT: Forms a DNA-binding heterodimer with transcription factor
CC       PBX1. {ECO:0000269|PubMed:7791786}.
CC   -!- INTERACTION:
CC       P09632; P41778: Pbx1; NbExp=4; IntAct=EBI-925374, EBI-6996259;
CC   -!- SUBCELLULAR LOCATION: Nucleus.
CC   -!- SIMILARITY: Belongs to the Antp homeobox family. {ECO:0000305}.
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DR   EMBL; X13961; CAA32141.1; -; Genomic_DNA.
DR   EMBL; X13721; CAA32000.1; -; mRNA.
DR   EMBL; M18399; AAA88246.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; X54077; CAA38014.1; -; Genomic_DNA.
DR   CCDS; CCDS25293.1; -.
DR   PIR; S03712; WJMS24.
DR   RefSeq; NP_034591.1; NM_010461.2.
DR   RefSeq; XP_017169784.1; XM_017314295.1.
DR   RefSeq; XP_017169785.1; XM_017314296.1.
DR   RefSeq; XP_017169786.1; XM_017314297.1.
DR   AlphaFoldDB; P09632; -.
DR   SMR; P09632; -.
DR   BioGRID; 200382; 14.
DR   DIP; DIP-35618N; -.
DR   IntAct; P09632; 15.
DR   STRING; 10090.ENSMUSP00000052496; -.
DR   iPTMnet; P09632; -.
DR   PhosphoSitePlus; P09632; -.
DR   MaxQB; P09632; -.
DR   PaxDb; 10090-ENSMUSP00000052496; -.
DR   ProteomicsDB; 273231; -.
DR   Antibodypedia; 30298; 227 antibodies from 27 providers.
DR   DNASU; 15416; -.
DR   Ensembl; ENSMUST00000052650.4; ENSMUSP00000052496.3; ENSMUSG00000056648.6.
DR   Ensembl; ENSMUST00000168043.2; ENSMUSP00000128136.2; ENSMUSG00000056648.6.
DR   GeneID; 15416; -.
DR   KEGG; mmu:15416; -.
DR   UCSC; uc007lbp.1; mouse.
DR   AGR; MGI:96189; -.
DR   CTD; 3218; -.
DR   MGI; MGI:96189; Hoxb8.
DR   VEuPathDB; HostDB:ENSMUSG00000056648; -.
DR   eggNOG; KOG0489; Eukaryota.
DR   GeneTree; ENSGT00940000161529; -.
DR   HOGENOM; CLU_061398_1_0_1; -.
DR   InParanoid; P09632; -.
DR   OMA; SEDCCEK; -.
DR   OrthoDB; 728401at2759; -.
DR   PhylomeDB; P09632; -.
DR   TreeFam; TF316310; -.
DR   BioGRID-ORCS; 15416; 1 hit in 81 CRISPR screens.
DR   PRO; PR:P09632; -.
DR   Proteomes; UP000000589; Chromosome 11.
DR   RNAct; P09632; Protein.
DR   Bgee; ENSMUSG00000056648; Expressed in lumbar subsegment of spinal cord and 108 other cell types or tissues.
DR   ExpressionAtlas; P09632; baseline and differential.
DR   GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR   GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central.
DR   GO; GO:0001227; F:DNA-binding transcription repressor activity, RNA polymerase II-specific; IDA:NTNU_SB.
DR   GO; GO:0000977; F:RNA polymerase II transcription regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IMP:NTNU_SB.
DR   GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI.
DR   GO; GO:0008344; P:adult locomotory behavior; IMP:MGI.
DR   GO; GO:0009952; P:anterior/posterior pattern specification; IMP:MGI.
DR   GO; GO:0021516; P:dorsal spinal cord development; IMP:MGI.
DR   GO; GO:0048704; P:embryonic skeletal system morphogenesis; IMP:MGI.
DR   GO; GO:0007625; P:grooming behavior; IMP:MGI.
DR   GO; GO:0045638; P:negative regulation of myeloid cell differentiation; IDA:UniProtKB.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; IDA:NTNU_SB.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:0019233; P:sensory perception of pain; IMP:MGI.
DR   GO; GO:0048705; P:skeletal system morphogenesis; IMP:MGI.
DR   CDD; cd00086; homeodomain; 1.
DR   Gene3D; 1.10.10.60; Homeodomain-like; 1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR001827; Homeobox_Antennapedia_CS.
DR   InterPro; IPR017970; Homeobox_CS.
DR   InterPro; IPR001356; Homeobox_dom.
DR   InterPro; IPR020479; Homeobox_metazoa.
DR   InterPro; IPR000047; HTH_motif.
DR   PANTHER; PTHR46166; HOMEOBOX DOMAIN-CONTAINING PROTEIN; 1.
DR   PANTHER; PTHR46166:SF2; HOMEOBOX PROTEIN HOX-B8; 1.
DR   Pfam; PF00046; Homeodomain; 1.
DR   PRINTS; PR00024; HOMEOBOX.
DR   PRINTS; PR00031; HTHREPRESSR.
DR   SMART; SM00389; HOX; 1.
DR   SUPFAM; SSF46689; Homeodomain-like; 1.
DR   PROSITE; PS00032; ANTENNAPEDIA; 1.
DR   PROSITE; PS00027; HOMEOBOX_1; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
DR   Genevisible; P09632; MM.
PE   1: Evidence at protein level;
KW   Developmental protein; DNA-binding; Homeobox; Nucleus; Reference proteome;
KW   Transcription; Transcription regulation.
FT   CHAIN           1..243
FT                   /note="Homeobox protein Hox-B8"
FT                   /id="PRO_0000200149"
FT   DNA_BIND        146..205
FT                   /note="Homeobox"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT   REGION          203..243
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOTIF           134..139
FT                   /note="Antp-type hexapeptide"
SQ   SEQUENCE   243 AA;  27618 MW;  368964C77884D21F CRC64;
     MSSYFVNSLF SKYKTGESLR PNYYDCGFAQ DLGGRPTVVY GPSSGGSFQH PSQIQEFYHG
     PSSLSTAPYQ QNPCAVACHG DPGNFYGYDP LQRQSLFGAQ DPDLVQYADC KLAAASGLGE
     EAEGSEQSPS PTQLFPWMRP QAAAGRRRGR QTYSRYQTLE LEKEFLFNPY LTRKRRIEVS
     HALGLTERQV KIWFQNRRMK WKKENNKDKF PSSKCEQEEL EKEKLERAPE TAEQGDAQKG
     DKK
//