Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Homeobox protein Hox-A9

Gene

Hoxa9

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Required for induction of E-selectin and VCAM-1, on the endothelial cells surface at sites of inflammation (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi205 – 264HomeoboxPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

GO - Biological processi

  • anterior/posterior pattern specification Source: MGI
  • definitive hemopoiesis Source: MGI
  • embryonic forelimb morphogenesis Source: MGI
  • embryonic skeletal system development Source: MGI
  • endothelial cell activation Source: MGI
  • male gonad development Source: MGI
  • mammary gland development Source: MGI
  • negative regulation of myeloid cell differentiation Source: UniProtKB
  • positive regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
  • proximal/distal pattern formation Source: MGI
  • regulation of gene expression Source: MGI
  • single fertilization Source: MGI
  • spermatogenesis Source: MGI
  • uterus development Source: MGI

Keywordsi

Molecular functionDevelopmental protein, DNA-binding
Biological processTranscription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Homeobox protein Hox-A9
Alternative name(s):
Homeobox protein Hox-1.7
Gene namesi
Name:Hoxa9
Synonyms:Hox-1.7, Hoxa-9
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:96180. Hoxa9.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002000821 – 271Homeobox protein Hox-A9Add BLAST271

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei139Symmetric dimethylarginineBy similarity1

Post-translational modificationi

Methylated on Arg-140 by PRMT5; methylation is critical for E-selectin induction.By similarity

Keywords - PTMi

Methylation

Proteomic databases

MaxQBiP09631.
PaxDbiP09631.
PRIDEiP09631.

PTM databases

iPTMnetiP09631.
PhosphoSitePlusiP09631.

Expressioni

Tissue specificityi

Expressed in high level in the embryonic and adult spinal cord with a preference in the posterior region.

Gene expression databases

BgeeiENSMUSG00000038227.
CleanExiMM_HOXA9.
GenevisibleiP09631. MM.

Interactioni

Subunit structurei

Transiently interacts with PRMT5 in TNF-alpha stimulated endothelial cells.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi200373. 13 interactors.
CORUMiP09631.
DIPiDIP-35619N.
IntActiP09631. 2 interactors.
MINTiMINT-210007.
STRINGi10090.ENSMUSP00000046939.

Structurei

Secondary structure

1271
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni195 – 198Combined sources4
Helixi214 – 226Combined sources13
Helixi232 – 242Combined sources11
Helixi246 – 267Combined sources22

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1PUFX-ray1.90A193-269[»]
ProteinModelPortaliP09631.
SMRiP09631.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09631.

Family & Domainsi

Sequence similaritiesi

Belongs to the Abd-B homeobox family.Curated

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG0487. Eukaryota.
ENOG4111FJP. LUCA.
GeneTreeiENSGT00760000118834.
HOGENOMiHOG000231180.
HOVERGENiHBG016849.
InParanoidiP09631.
KOiK09294.
OMAiWSHVPAQ.
OrthoDBiEOG091G0IP5.
PhylomeDBiP09631.
TreeFamiTF317819.

Family and domain databases

CDDicd00086. homeodomain. 1 hit.
InterProiView protein in InterPro
IPR009057. Homeobox-like.
IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR006711. Hox9_activation_N.
IPR017112. HXA9/HXB9/HXC9.
PfamiView protein in Pfam
PF00046. Homeobox. 1 hit.
PF04617. Hox9_act. 1 hit.
PIRSFiPIRSF037109. Homeobox_Hox9. 1 hit.
PRINTSiPR00024. HOMEOBOX.
SMARTiView protein in SMART
SM00389. HOX. 1 hit.
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiView protein in PROSITE
PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform HoxA-9 (identifier: P09631-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATTGALGNY YVDSFLLGAD AADELGAGRY APGTLGQPPR QAAALAEHPD
60 70 80 90 100
FSPCSFQSKA AVFGASWNPV HAAGANAVPA AVYHHHHHPY VHPQAPVAAA
110 120 130 140 150
APDGRYMRSW LEPTPGALSF AGLPSSRPYG IKPEPLSARR GDCPTLDTHT
160 170 180 190 200
LSLTDYACGS PPVDREKQPS EGAFSENNAE NESGGDKPPI DPNNPAANWL
210 220 230 240 250
HARSTRKKRC PYTKHQTLEL EKEFLFNMYL TRDRRYEVAR LLNLTERQVK
260 270
IWFQNRRMKM KKINKDRAKD E
Length:271
Mass (Da):29,917
Last modified:July 15, 1999 - v2
Checksum:iC1E679D9CBF677B0
GO
Isoform HoxA-9T (identifier: P09631-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     105-105: R → S
     106-271: Missing.

Show »
Length:105
Mass (Da):10,684
Checksum:i923A157D0AA22579
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_002382105R → S in isoform HoxA-9T. 1 Publication1
Alternative sequenceiVSP_002383106 – 271Missing in isoform HoxA-9T. 1 PublicationAdd BLAST166

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB005457 mRNA. Translation: BAA25800.1.
AB005458 mRNA. Translation: BAA25801.1.
AB008914 Genomic DNA. Translation: BAA25802.1.
BC055059 mRNA. Translation: AAH55059.1.
M28449 mRNA. Translation: AAA78790.1.
CCDSiCCDS20146.1. [P09631-1]
PIRiA31400.
JC6553.
RefSeqiNP_034586.1. NM_010456.3. [P09631-1]
UniGeneiMm.4694.

Genome annotation databases

EnsembliENSMUST00000048680; ENSMUSP00000046939; ENSMUSG00000038227. [P09631-1]
ENSMUST00000114425; ENSMUSP00000110068; ENSMUSG00000038227. [P09631-2]
GeneIDi15405.
KEGGimmu:15405.
UCSCiuc009byl.2. mouse. [P09631-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiHXA9_MOUSE
AccessioniPrimary (citable) accession number: P09631
Secondary accession number(s): O70154, O70155
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 15, 1999
Last modified: September 27, 2017
This is version 164 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Vertebrate homeotic Hox proteins
    Nomenclature of vertebrate homeotic Hox proteins and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families