Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Homeobox protein Hox-A9

Gene

Hoxa9

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Required for induction of E-selectin and VCAM-1, on the endothelial cells surface at sites of inflammation (By similarity).By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi205 – 26460HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • anterior/posterior pattern specification Source: MGI
  • definitive hemopoiesis Source: MGI
  • embryonic forelimb morphogenesis Source: MGI
  • embryonic skeletal system development Source: MGI
  • endothelial cell activation Source: MGI
  • male gonad development Source: MGI
  • mammary gland development Source: MGI
  • negative regulation of myeloid cell differentiation Source: UniProtKB
  • proximal/distal pattern formation Source: MGI
  • regulation of gene expression Source: MGI
  • regulation of transcription, DNA-templated Source: UniProtKB-KW
  • single fertilization Source: MGI
  • spermatogenesis Source: MGI
  • transcription, DNA-templated Source: UniProtKB-KW
  • uterus development Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Homeobox protein Hox-A9
Alternative name(s):
Homeobox protein Hox-1.7
Gene namesi
Name:Hoxa9
Synonyms:Hox-1.7, Hoxa-9
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:96180. Hoxa9.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: MGI
  • nucleoplasm Source: MGI
  • nucleus Source: MGI
  • transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 271271Homeobox protein Hox-A9PRO_0000200082Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei139 – 1391Symmetric dimethylarginineBy similarity

Post-translational modificationi

Methylated on Arg-140 by PRMT5; methylation is critical for E-selectin induction.By similarity

Keywords - PTMi

Methylation

Proteomic databases

MaxQBiP09631.
PRIDEiP09631.

PTM databases

PhosphoSiteiP09631.

Expressioni

Tissue specificityi

Expressed in high level in the embryonic and adult spinal cord with a preference in the posterior region.

Gene expression databases

BgeeiP09631.
CleanExiMM_HOXA9.
GenevisibleiP09631. MM.

Interactioni

Subunit structurei

Transiently interacts with PRMT5 in TNF-alpha stimulated endothelial cells.By similarity

Protein-protein interaction databases

BioGridi200373. 13 interactions.
DIPiDIP-35619N.
IntActiP09631. 1 interaction.
MINTiMINT-210007.
STRINGi10090.ENSMUSP00000046939.

Structurei

Secondary structure

1
271
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Turni195 – 1984Combined sources
Helixi214 – 22613Combined sources
Helixi232 – 24211Combined sources
Helixi246 – 26722Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PUFX-ray1.90A193-269[»]
ProteinModelPortaliP09631.
SMRiP09631. Positions 193-269.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09631.

Family & Domainsi

Sequence similaritiesi

Belongs to the Abd-B homeobox family.Curated
Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG286554.
GeneTreeiENSGT00760000118834.
HOGENOMiHOG000231180.
HOVERGENiHBG016849.
InParanoidiP09631.
KOiK09294.
OMAiVPAVYHP.
OrthoDBiEOG722J9F.
PhylomeDBiP09631.
TreeFamiTF317819.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR017112. Homeobox_Hox9.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
IPR006711. Hox9_activation_N.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF04617. Hox9_act. 1 hit.
[Graphical view]
PIRSFiPIRSF037109. Homeobox_Hox9. 1 hit.
PRINTSiPR00024. HOMEOBOX.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform HoxA-9 (identifier: P09631-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATTGALGNY YVDSFLLGAD AADELGAGRY APGTLGQPPR QAAALAEHPD
60 70 80 90 100
FSPCSFQSKA AVFGASWNPV HAAGANAVPA AVYHHHHHPY VHPQAPVAAA
110 120 130 140 150
APDGRYMRSW LEPTPGALSF AGLPSSRPYG IKPEPLSARR GDCPTLDTHT
160 170 180 190 200
LSLTDYACGS PPVDREKQPS EGAFSENNAE NESGGDKPPI DPNNPAANWL
210 220 230 240 250
HARSTRKKRC PYTKHQTLEL EKEFLFNMYL TRDRRYEVAR LLNLTERQVK
260 270
IWFQNRRMKM KKINKDRAKD E
Length:271
Mass (Da):29,917
Last modified:July 15, 1999 - v2
Checksum:iC1E679D9CBF677B0
GO
Isoform HoxA-9T (identifier: P09631-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     105-105: R → S
     106-271: Missing.

Show »
Length:105
Mass (Da):10,684
Checksum:i923A157D0AA22579
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei105 – 1051R → S in isoform HoxA-9T. 1 PublicationVSP_002382
Alternative sequencei106 – 271166Missing in isoform HoxA-9T. 1 PublicationVSP_002383Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB005457 mRNA. Translation: BAA25800.1.
AB005458 mRNA. Translation: BAA25801.1.
AB008914 Genomic DNA. Translation: BAA25802.1.
BC055059 mRNA. Translation: AAH55059.1.
M28449 mRNA. Translation: AAA78790.1.
CCDSiCCDS20146.1. [P09631-1]
PIRiA31400.
JC6553.
RefSeqiNP_034586.1. NM_010456.3. [P09631-1]
UniGeneiMm.4694.

Genome annotation databases

EnsembliENSMUST00000048680; ENSMUSP00000046939; ENSMUSG00000038227. [P09631-1]
ENSMUST00000114425; ENSMUSP00000110068; ENSMUSG00000038227. [P09631-2]
GeneIDi15405.
KEGGimmu:15405.
UCSCiuc009byl.1. mouse. [P09631-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB005457 mRNA. Translation: BAA25800.1.
AB005458 mRNA. Translation: BAA25801.1.
AB008914 Genomic DNA. Translation: BAA25802.1.
BC055059 mRNA. Translation: AAH55059.1.
M28449 mRNA. Translation: AAA78790.1.
CCDSiCCDS20146.1. [P09631-1]
PIRiA31400.
JC6553.
RefSeqiNP_034586.1. NM_010456.3. [P09631-1]
UniGeneiMm.4694.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1PUFX-ray1.90A193-269[»]
ProteinModelPortaliP09631.
SMRiP09631. Positions 193-269.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200373. 13 interactions.
DIPiDIP-35619N.
IntActiP09631. 1 interaction.
MINTiMINT-210007.
STRINGi10090.ENSMUSP00000046939.

PTM databases

PhosphoSiteiP09631.

Proteomic databases

MaxQBiP09631.
PRIDEiP09631.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000048680; ENSMUSP00000046939; ENSMUSG00000038227. [P09631-1]
ENSMUST00000114425; ENSMUSP00000110068; ENSMUSG00000038227. [P09631-2]
GeneIDi15405.
KEGGimmu:15405.
UCSCiuc009byl.1. mouse. [P09631-1]

Organism-specific databases

CTDi3205.
MGIiMGI:96180. Hoxa9.

Phylogenomic databases

eggNOGiNOG286554.
GeneTreeiENSGT00760000118834.
HOGENOMiHOG000231180.
HOVERGENiHBG016849.
InParanoidiP09631.
KOiK09294.
OMAiVPAVYHP.
OrthoDBiEOG722J9F.
PhylomeDBiP09631.
TreeFamiTF317819.

Miscellaneous databases

EvolutionaryTraceiP09631.
NextBioi288114.
PROiP09631.
SOURCEiSearch...

Gene expression databases

BgeeiP09631.
CleanExiMM_HOXA9.
GenevisibleiP09631. MM.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR017112. Homeobox_Hox9.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
IPR006711. Hox9_activation_N.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF04617. Hox9_act. 1 hit.
[Graphical view]
PIRSFiPIRSF037109. Homeobox_Hox9. 1 hit.
PRINTSiPR00024. HOMEOBOX.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Analysis of the murine Hoxa-9 cDNA: an alternatively spliced transcript encodes a truncated protein lacking the homeodomain."
    Fujimoto S., Araki K., Chisaka O., Araki M., Takagi K., Yamamura K.
    Gene 209:77-85(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS HOXA-9 AND HOXA-9T).
    Strain: C57BL/6 and ICR.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM HOXA-9).
    Strain: FVB/N.
    Tissue: Colon.
  3. "Murine Hox-1.7 homeo-box gene: cloning, chromosomal location, and expression."
    Rubin M.R., King W., Toth L.E., Sawczuk I.S., Levine M.S., D'Eustachio P., Nguyen-Huu M.C.
    Mol. Cell. Biol. 7:3836-3841(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE OF 180-271 (HOXA-9).
  4. Erratum
    Rubin M.R., King W., Toth L.E., Sawczuk I.S., Levine M.S., D'Eustachio P., Nguyen-Huu M.C.
    Mol. Cell. Biol. 8:5593-5593(1988)
    Cited for: SEQUENCE REVISION.

Entry informationi

Entry nameiHXA9_MOUSE
AccessioniPrimary (citable) accession number: P09631
Secondary accession number(s): O70154, O70155
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 15, 1999
Last modified: June 24, 2015
This is version 150 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Vertebrate homeotic Hox proteins
    Nomenclature of vertebrate homeotic Hox proteins and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.