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Protein

Plasma membrane ATPase 1

Gene

pma1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The plasma membrane ATPase of plants and fungi is a hydrogen ion pump. The proton gradient it generates drives the active transport of nutrients by H+-symport. The resulting external acidification and/or internal alkinization may mediate growth responses.

Catalytic activityi

ATP + H2O + H+(In) = ADP + phosphate + H+(Out).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei376 – 37614-aspartylphosphate intermediateBy similarity
Metal bindingi632 – 6321MagnesiumBy similarity
Metal bindingi636 – 6361MagnesiumBy similarity

GO - Molecular functioni

  • ATPase activity Source: PomBase
  • ATP binding Source: UniProtKB-KW
  • hydrogen-exporting ATPase activity, phosphorylative mechanism Source: PomBase
  • metal ion binding Source: UniProtKB-KW
  • proton binding Source: PomBase

GO - Biological processi

  • ATP biosynthetic process Source: InterPro
  • hydrogen ion transmembrane transport Source: GOC
  • proton transport Source: PomBase
  • regulation of intracellular pH Source: PomBase
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Hydrogen ion transport, Ion transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Plasma membrane ATPase 1 (EC:3.6.3.6)
Alternative name(s):
Proton pump 1
Gene namesi
Name:pma1
ORF Names:SPAC1071.10c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC1071.10c.
PomBaseiSPAC1071.10c. pma1.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 113113CytoplasmicSequence analysisAdd
BLAST
Transmembranei114 – 13421Helical; Name=1Sequence analysisAdd
BLAST
Topological domaini135 – 1384ExtracellularSequence analysis
Transmembranei139 – 15820Helical; Name=2Sequence analysisAdd
BLAST
Topological domaini159 – 289131CytoplasmicSequence analysisAdd
BLAST
Transmembranei290 – 31122Helical; Name=3Sequence analysisAdd
BLAST
Topological domaini312 – 32211ExtracellularSequence analysisAdd
BLAST
Transmembranei323 – 34523Helical; Name=4Sequence analysisAdd
BLAST
Topological domaini346 – 717372CytoplasmicSequence analysisAdd
BLAST
Transmembranei718 – 73619Helical; Name=5Sequence analysisAdd
BLAST
Topological domaini737 – 75216ExtracellularSequence analysisAdd
BLAST
Transmembranei753 – 77220Helical; Name=6Sequence analysisAdd
BLAST
Topological domaini773 – 82452CytoplasmicSequence analysisAdd
BLAST
Transmembranei825 – 84521Helical; Name=7Sequence analysisAdd
BLAST
Topological domaini846 – 85813ExtracellularSequence analysisAdd
BLAST
Transmembranei859 – 87517Helical; Name=8Sequence analysisAdd
BLAST
Topological domaini876 – 91944CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • Golgi apparatus Source: PomBase
  • integral component of membrane Source: PomBase
  • integral component of plasma membrane Source: PomBase
  • plasma membrane Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 919919Plasma membrane ATPase 1PRO_0000046269Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei89 – 891Phosphoserine1 Publication
Modified residuei494 – 4941Phosphoserine1 Publication
Modified residuei899 – 8991Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP09627.

PTM databases

iPTMnetiP09627.

Interactioni

Protein-protein interaction databases

BioGridi279117. 7 interactions.
DIPiDIP-59125N.
MINTiMINT-4687131.

Structurei

3D structure databases

ProteinModelPortaliP09627.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000160005.
InParanoidiP09627.
KOiK01535.
OMAiDRINNES.
OrthoDBiEOG789CKN.
PhylomeDBiP09627.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006534. P-type_ATPase_IIIA.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
PRINTSiPR00120. HATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
TIGRFAMsiTIGR01647. ATPase-IIIA_H. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P09627-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MADNAGEYHD AEKHAPEQQA PPPQQPAHAA APAQDDEPDD DIDALIEELF
60 70 80 90 100
SEDVQEEQED NDDAPAAGEA KAVPEELLQT DMNTGLTMSE VEERRKKYGL
110 120 130 140 150
NQMKEELENP FLKFIMFFVG PIQFVMEMAA ALAAGLRDWV DFGVICALLM
160 170 180 190 200
LNAVVGFVQE YQAGSIVDEL KKSLALKAVV IREGQVHELE ANEVVPGDIL
210 220 230 240 250
KLDEGTIICA DGRVVTPDVH LQVDQSAITG ESLAVDKHYG DPTFASSGVK
260 270 280 290 300
RGEGLMVVTA TGDSTFVGRA ASLVNAAAGG TGHFTEVLNG IGTILLVLVL
310 320 330 340 350
LTLFCIYTAA FYRSVRLARL LEYTLAITII GVPVGLPAVV TTTMAVGAAY
360 370 380 390 400
LAEKQAIVQK LSAIESLAGV EVLCSDKTGT LTKNKLSLGE PFTVSGVSGD
410 420 430 440 450
DLVLTACLAA SRKRKGLDAI DKAFLKALKN YPGPRSMLTK YKVIEFQPFD
460 470 480 490 500
PVSKKVTAYV QAPDGTRITC VKGAPLWVLK TVEEDHPIPE DVLSAYKDKV
510 520 530 540 550
GDLASRGYRS LGVARKIEGQ HWEIMGIMPC SDPPRHDTAR TISEAKRLGL
560 570 580 590 600
RVKMLTGDAV DIAKETARQL GMGTNIYNAE RLGLTGGGNM PGSEVYDFVE
610 620 630 640 650
AADGFGEVFP QHKYAVVDIL QQRGYLVAMT GDGVNDAPSL KKADTGIAVE
660 670 680 690 700
GATDAARSAA DIVFLAPGLS AIIDALKTSR QIFHRMYSYV VYRIALSLHL
710 720 730 740 750
EIFLGLWLII RNQLLNLELV VFIAIFADVA TLAIAYDNAP YSMKPVKWNL
760 770 780 790 800
PRLWGLSTVI GIVLAIGTWI TNTTMIAQGQ NRGIVQNFGV QDEVLFLEIS
810 820 830 840 850
LTENWLIFVT RCNGPFWSSI PSWQLSGAVL AVDILATMFC IFGWFKGGHQ
860 870 880 890 900
TSIVAVLRIW MYSFGIFCIM AGTYYILSES AGFDRMMNGK PKESRNQRSI
910
EDLVVALQRT STRHEKGDA
Length:919
Mass (Da):99,884
Last modified:July 1, 1989 - v1
Checksum:i0AE31B92ED607CC7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03498 Genomic DNA. Translation: AAA35324.1.
CU329670 Genomic DNA. Translation: CAB59886.1.
PIRiA28454. PXZP1P.
RefSeqiNP_594360.1. NM_001019781.2.

Genome annotation databases

EnsemblFungiiSPAC1071.10c.1; SPAC1071.10c.1:pep; SPAC1071.10c.
GeneIDi2542664.
KEGGispo:SPAC1071.10c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J03498 Genomic DNA. Translation: AAA35324.1.
CU329670 Genomic DNA. Translation: CAB59886.1.
PIRiA28454. PXZP1P.
RefSeqiNP_594360.1. NM_001019781.2.

3D structure databases

ProteinModelPortaliP09627.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi279117. 7 interactions.
DIPiDIP-59125N.
MINTiMINT-4687131.

PTM databases

iPTMnetiP09627.

Proteomic databases

MaxQBiP09627.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC1071.10c.1; SPAC1071.10c.1:pep; SPAC1071.10c.
GeneIDi2542664.
KEGGispo:SPAC1071.10c.

Organism-specific databases

EuPathDBiFungiDB:SPAC1071.10c.
PomBaseiSPAC1071.10c. pma1.

Phylogenomic databases

HOGENOMiHOG000160005.
InParanoidiP09627.
KOiK01535.
OMAiDRINNES.
OrthoDBiEOG789CKN.
PhylomeDBiP09627.

Miscellaneous databases

PROiP09627.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR023214. HAD-like_dom.
IPR006534. P-type_ATPase_IIIA.
IPR001757. P_typ_ATPase.
[Graphical view]
PfamiPF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
[Graphical view]
PRINTSiPR00120. HATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 2 hits.
TIGRFAMsiTIGR01647. ATPase-IIIA_H. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Mutation of a conserved glycine residue modifies the vanadate sensitivity of the plasma membrane H+-ATPase from Schizosaccharomyces pombe."
    Ghislain M., Schlesser A., Goffeau A.
    J. Biol. Chem. 262:17549-17555(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  3. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-89; SER-494 AND SER-899, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiPMA1_SCHPO
AccessioniPrimary (citable) accession number: P09627
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: June 8, 2016
This is version 142 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.