Potassium-transporting ATPase alpha chain 1

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Reviewed, UniProtKB/Swiss-Prot P09626 (ATP4A_RAT)

Last modified October 13, 2009. Version 96. History...

Clusters with 100%, 90%, 50% identity |

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Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein names

Recommended name:
    Potassium-transporting ATPase alpha chain 1
    EC=3.6.3.10
Alternative name(s):
    Proton pump
    Gastric H(+)/K(+) ATPase subunit alpha

Gene names

Name:	Atp4a
Synonyms:	Hka

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

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Protein attributes

Sequence length	1033 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

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General annotation (Comments)

Function	Catalyzes the hydrolysis of ATP coupled with the exchange of H⁺ and K⁺ ions across the plasma membrane. Responsible for acid production in the stomach.
Catalytic activity	ATP + H₂O + H⁺(In) + K⁺(Out) = ADP + phosphate + H⁺(Out) + K⁺(In).
Subunit structure	Composed of two subunits: alpha (catalytic) and beta.
Subcellular location	Membrane; Multi-pass membrane protein.
Sequence similarities	Belongs to the cation transport ATPase (P-type) family. Type IIC subfamily.

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Ontologies

Keywords
Biological process	Hydrogen ion transport Ion transport Potassium transport Transport
Cellular component	Membrane
Domain	Transmembrane
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding Potassium
Molecular function	Hydrolase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	ATP biosynthetic process Inferred from electronic annotation. Source: InterPro ATP hydrolysis coupled proton transport Inferred from electronic annotation. Source: InterPro gastric acid secretion Ref.1 Traceable author statement. Source: RGD potassium ion transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	apical plasma membrane Inferred from direct assay. Source: UniProtKB integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW hydrogen:potassium-exchanging ATPase activity Ref.1 Traceable author statement. Source: RGD magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW potassium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

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Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

Removed By similarity

Chain

2 – 1033

1032

Potassium-transporting ATPase alpha chain 1

PRO_0000046257

Regions

Topological domain

2 – 96

Cytoplasmic Potential

Transmembrane

97 – 117

Potential

Topological domain

118 – 140

Lumenal Potential

Transmembrane

141 – 161

Potential

Topological domain

162 – 297

136

Cytoplasmic Potential

Transmembrane

298 – 317

Potential

Topological domain

318 – 329

Lumenal Potential

Transmembrane

330 – 347

Potential

Topological domain

348 – 781

434

Cytoplasmic Potential

Transmembrane

782 – 801

Potential

Topological domain

802 – 811

Lumenal Potential

Transmembrane

812 – 832

Potential

Topological domain

833 – 852

Cytoplasmic Potential

Transmembrane

853 – 875

Potential

Topological domain

876 – 927

Lumenal Potential

Transmembrane

928 – 947

Potential

Topological domain

948 – 961

Cytoplasmic Potential

Transmembrane

962 – 980

Potential

Topological domain

981 – 995

Lumenal Potential

Transmembrane

996 – 1016

Potential

Topological domain

1017 – 1033

Cytoplasmic Potential

Sites

Active site

385

4-aspartylphosphate intermediate By similarity

Metal binding

726

Magnesium By similarity

Metal binding

730

Magnesium By similarity

Amino acid modifications

Modified residue

952

Phosphoserine; by PKA By similarity

Experimental info

Sequence conflict

K → KA in AAA72354. Ref.4

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Sequences

Sequence

Length

Mass (Da)

Tools

P09626-1 [UniParc].

Last modified January 23, 2007. Version 3.
Checksum: 5A8835BA0CAF987E
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FASTA

1,033

114,038

        10         20         30         40         50         60 
MGKENYELYS VELGTGPGGD MAAKMSKKKA GGGGGKKKEK LENMKKEMEM NDHQLSVSEL 

        70         80         90        100        110        120 
EQKYQTSATK GLKASLAAEL LLRDGPNALR PPRGTPEYVK FARQLAGGLQ CLMWVAAAIC 

       130        140        150        160        170        180 
LIAFAIQASE GDLTTDDNLY LALALIAVVV VTGCFGYYQE FKSTNIIASF KNLVPQQATV 

       190        200        210        220        230        240 
IRDGDKFQIN ADQLVVGDLV EMKGGDRVPA DIRILSAQGC KVDNSSLTGE SEPQTRSPEC 

       250        260        270        280        290        300 
THESPLETRN IAFFSTMCLE GTAQGLVVST GDRTIIGRIA SLASGVENEK TPIAIEIEHF 

       310        320        330        340        350        360 
VDIIAGLAIL FGATFFVVAM CIGYTFLRAM VFFMAIVVAY VPEGLLATVT VCLSLTAKRL 

       370        380        390        400        410        420 
ASKNCVVKNL EAVETLGSTS VICSDKTGTL TQNRMTVSHL WFDNHIHTAD TTEDQSGQTF 

       430        440        450        460        470        480 
DQSSETWRAL CRVLTLCNRA AFKSGQDAVP VPKRIVIGDA SETALLKFSE LTLGNAMGYR 

       490        500        510        520        530        540 
DRFPKVCEIP FNSTNKFQLS IHTLEDPRDP RHLLVMKGAP ERVLERCSSI LIKGQELPLD 

       550        560        570        580        590        600 
EQWREAFQTA YLSLGGLGER VLGFCQLYLN EKDYPPGYTF DVEAMNFPSS GLCFAGLVSM 

       610        620        630        640        650        660 
IDPPRATVPD AVLKCRTAGI RVIMVTGDHP ITAKAIAASV GIISEGSETV EDIAARLRMP 

       670        680        690        700        710        720 
VDQVNKKDAR ACVINGMQLK DMDPSELVEA LRTHPEMVFA RTSPQQKLVI VESCQRLGAI 

       730        740        750        760        770        780 
VAVTGDGVND SPALKKADIG VAMGIAGSDA AKNAADMILL DDNFASIVTG VEQGRLIFDN 

       790        800        810        820        830        840 
LKKSIAYTLT KNIPELTPYL IYITVSVPLP LGCITILFIE LCTDIFPSVS LAYEKAESDI 

       850        860        870        880        890        900 
MHLRPRNPRR DRLVNEPLAA YSYFQIGAIQ SFAGFADYFT AMAQEGWFPL LCVGLRPQWE 

       910        920        930        940        950        960 
DHHLQDLQDS YGQEWTFGQR LYQQYTCYTV FFISIEMCQI ADVLIRKTRR LSAFQQGFFR 

       970        980        990       1000       1010       1020 
NRILVIAIVF QVCIGCFLCY CPGMPNIFNF MPIRFQWWLV PMPFGLLIFV YDEIRKLGVR 

      1030 
CCPGSWWDQE LYY

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References

[1]	"Molecular cloning of the rat stomach (H+ + K+)-ATPase." Shull G.E., Lingrel J.B. J. Biol. Chem. 261:16788-16791(1986) [PubMed: 3023364] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Shull G.E. Submitted (FEB-1987) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"Expression and cellular localization of mRNA encoding the 'gastric' isoform of H(+)-K(+)-ATPase alpha-subunit in rat kidney." Ahn K.Y., Kone B.C. Am. J. Physiol. 268:F99-F109(1995) [PubMed: 7840253] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 54-507.
[4]	Song I., Mortell P., Gantz I., Marino L.R., Yamada T. Submitted (FEB-1993) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE OF 1-50. Tissue: Liver.
[5]	"Control region and gastric specific transcription of the rat H+,K(+)-ATPase alpha subunit gene." Oshiman K., Motajima K., Mahmood S., Shimada A., Tamura S., Maeda M., Futai M. FEBS Lett. 281:250-254(1991) [PubMed: 1849840] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 177-260 AND 436-466. Strain: Sprague-Dawley.
+	Additional computationally mapped references.

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Cross-references

Sequence databases
	J02649 mRNA. Translation: AAA66036.1. S74801 mRNA. Translation: AAP31528.1. L11569 Genomic DNA. Translation: AAA72354.1. X61934 Genomic DNA. Translation: CAA43938.1. X61935 Genomic DNA. Translation: CAA43939.1.
IPI	IPI00365705.
PIR	A25344.
UniGene	Rn.214529
3D structure databases
HSSP	HSSP built from PDB template 1IWC based on UniProtKB P19156.
ModBase	Search...
Protein-protein interaction databases
STRING	P09626.
PTM databases
PhosphoSite	P09626.
Genome annotation databases
Ensembl	ENSRNOT00000028508; ENSRNOP00000028508; ENSRNOG00000020985; Rattus norvegicus. [Genome view]
Organism-specific databases
RGD	2177. Atp4a.
Phylogenomic databases
HOVERGEN	P09626.
Enzyme and pathway databases
BRENDA	3.6.3.10. 248.
Gene expression databases
ArrayExpress	P09626.
Genevestigator	P09626.
GermOnline	ENSRNOG00000020985. Rattus norvegicus.
Family and domain databases
InterPro	IPR008250. ATPase_P-typ_ATPase-assoc-reg. IPR005775. ATPase_P-typ_cation-ex_asu_euk. IPR006069. ATPase_P-typ_cation-exchng_asu. IPR006068. ATPase_P-typ_cation-transptr_C. IPR004014. ATPase_P-typ_cation-transptr_N. IPR015127. ATPase_P-typ_H/K-transp_N. IPR001757. ATPase_P-typ_ion-transptr. IPR018303. ATPase_P-typ_P_site. IPR005834. Dehalogen-like_hydro. [Graphical view]
PANTHER	PTHR11939. ATPase_P. 1 hit.
Pfam	PF00689. Cation_ATPase_C. 1 hit. PF00690. Cation_ATPase_N. 1 hit. PF00122. E1-E2_ATPase. 1 hit. PF09040. H-K_ATPase_N. 1 hit. PF00702. Hydrolase. 1 hit. [Graphical view]
PRINTS	PR00119. CATATPASE. PR00121. NAKATPASE.
TIGRFAMs	TIGR01106. ATPase-IIC_X-K. 1 hit. TIGR01494. ATPase_P-type. 4 hits.
PROSITE	PS00154. ATPASE_E1_E2. 1 hit. [Graphical view]
ProtoNet	Search...

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Entry information

Entry name

ATP4A_RAT

Accession

Primary (citable) accession number: P09626
Secondary accession number(s): P70511

Q6LD86

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	January 23, 2007
Last modified:	October 13, 2009
This is version 96 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents