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P09626

- ATP4A_RAT

UniProt

P09626 - ATP4A_RAT

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Protein

Potassium-transporting ATPase alpha chain 1

Gene

Atp4a

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the hydrolysis of ATP coupled with the exchange of H+ and K+ ions across the plasma membrane. Responsible for acid production in the stomach.

Catalytic activityi

ATP + H2O + H+(In) + K+(Out) = ADP + phosphate + H+(Out) + K+(In).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei385 – 38514-aspartylphosphate intermediateBy similarity
Metal bindingi726 – 7261MagnesiumBy similarity
Metal bindingi730 – 7301MagnesiumBy similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. hydrogen:potassium-exchanging ATPase activity Source: RGD
  3. magnesium ion binding Source: InterPro

GO - Biological processi

  1. ATP biosynthetic process Source: InterPro
  2. ATP hydrolysis coupled proton transport Source: InterPro
  3. gastric acid secretion Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Hydrogen ion transport, Ion transport, Potassium transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium

Enzyme and pathway databases

BRENDAi3.6.3.10. 5301.

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium-transporting ATPase alpha chain 1 (EC:3.6.3.10)
Alternative name(s):
Gastric H(+)/K(+) ATPase subunit alpha
Proton pump
Gene namesi
Name:Atp4a
Synonyms:Hka
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi2177. Atp4a.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 9695CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei97 – 11721HelicalSequence AnalysisAdd
BLAST
Topological domaini118 – 14023LumenalSequence AnalysisAdd
BLAST
Transmembranei141 – 16121HelicalSequence AnalysisAdd
BLAST
Topological domaini162 – 297136CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei298 – 31720HelicalSequence AnalysisAdd
BLAST
Topological domaini318 – 32912LumenalSequence AnalysisAdd
BLAST
Transmembranei330 – 34718HelicalSequence AnalysisAdd
BLAST
Topological domaini348 – 781434CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei782 – 80120HelicalSequence AnalysisAdd
BLAST
Topological domaini802 – 81110LumenalSequence Analysis
Transmembranei812 – 83221HelicalSequence AnalysisAdd
BLAST
Topological domaini833 – 85220CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei853 – 87523HelicalSequence AnalysisAdd
BLAST
Topological domaini876 – 92752LumenalSequence AnalysisAdd
BLAST
Transmembranei928 – 94720HelicalSequence AnalysisAdd
BLAST
Topological domaini948 – 96114CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei962 – 98019HelicalSequence AnalysisAdd
BLAST
Topological domaini981 – 99515LumenalSequence AnalysisAdd
BLAST
Transmembranei996 – 101621HelicalSequence AnalysisAdd
BLAST
Topological domaini1017 – 103317CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. apical plasma membrane Source: UniProtKB
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 10331032Potassium-transporting ATPase alpha chain 1PRO_0000046257Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei6 – 61PhosphotyrosineBy similarity
Modified residuei9 – 91PhosphotyrosineBy similarity
Modified residuei26 – 261PhosphoserineBy similarity
Modified residuei952 – 9521Phosphoserine; by PKABy similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP09626.
PRIDEiP09626.

PTM databases

PhosphoSiteiP09626.

Expressioni

Gene expression databases

GenevestigatoriP09626.

Interactioni

Subunit structurei

Composed of two subunits: alpha (catalytic) and beta.

Structurei

3D structure databases

ProteinModelPortaliP09626.
SMRiP09626. Positions 1-33, 35-1033.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0474.
HOGENOMiHOG000265622.
HOVERGENiHBG004298.
InParanoidiP09626.
PhylomeDBiP09626.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR015127. ATPase_P-typ_H/K-transp_N.
IPR005775. ATPase_P-typ_Na/K_IIC.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF09040. H-K_ATPase_N. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 3 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09626-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MGKENYELYS VELGTGPGGD MAAKMSKKKA GGGGGKKKEK LENMKKEMEM
60 70 80 90 100
NDHQLSVSEL EQKYQTSATK GLKASLAAEL LLRDGPNALR PPRGTPEYVK
110 120 130 140 150
FARQLAGGLQ CLMWVAAAIC LIAFAIQASE GDLTTDDNLY LALALIAVVV
160 170 180 190 200
VTGCFGYYQE FKSTNIIASF KNLVPQQATV IRDGDKFQIN ADQLVVGDLV
210 220 230 240 250
EMKGGDRVPA DIRILSAQGC KVDNSSLTGE SEPQTRSPEC THESPLETRN
260 270 280 290 300
IAFFSTMCLE GTAQGLVVST GDRTIIGRIA SLASGVENEK TPIAIEIEHF
310 320 330 340 350
VDIIAGLAIL FGATFFVVAM CIGYTFLRAM VFFMAIVVAY VPEGLLATVT
360 370 380 390 400
VCLSLTAKRL ASKNCVVKNL EAVETLGSTS VICSDKTGTL TQNRMTVSHL
410 420 430 440 450
WFDNHIHTAD TTEDQSGQTF DQSSETWRAL CRVLTLCNRA AFKSGQDAVP
460 470 480 490 500
VPKRIVIGDA SETALLKFSE LTLGNAMGYR DRFPKVCEIP FNSTNKFQLS
510 520 530 540 550
IHTLEDPRDP RHLLVMKGAP ERVLERCSSI LIKGQELPLD EQWREAFQTA
560 570 580 590 600
YLSLGGLGER VLGFCQLYLN EKDYPPGYTF DVEAMNFPSS GLCFAGLVSM
610 620 630 640 650
IDPPRATVPD AVLKCRTAGI RVIMVTGDHP ITAKAIAASV GIISEGSETV
660 670 680 690 700
EDIAARLRMP VDQVNKKDAR ACVINGMQLK DMDPSELVEA LRTHPEMVFA
710 720 730 740 750
RTSPQQKLVI VESCQRLGAI VAVTGDGVND SPALKKADIG VAMGIAGSDA
760 770 780 790 800
AKNAADMILL DDNFASIVTG VEQGRLIFDN LKKSIAYTLT KNIPELTPYL
810 820 830 840 850
IYITVSVPLP LGCITILFIE LCTDIFPSVS LAYEKAESDI MHLRPRNPRR
860 870 880 890 900
DRLVNEPLAA YSYFQIGAIQ SFAGFADYFT AMAQEGWFPL LCVGLRPQWE
910 920 930 940 950
DHHLQDLQDS YGQEWTFGQR LYQQYTCYTV FFISIEMCQI ADVLIRKTRR
960 970 980 990 1000
LSAFQQGFFR NRILVIAIVF QVCIGCFLCY CPGMPNIFNF MPIRFQWWLV
1010 1020 1030
PMPFGLLIFV YDEIRKLGVR CCPGSWWDQE LYY
Length:1,033
Mass (Da):114,038
Last modified:January 23, 2007 - v3
Checksum:i5A8835BA0CAF987E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti3 – 31K → KA in AAA72354. 1 PublicationCurated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02649 mRNA. Translation: AAA66036.1.
S74801 mRNA. Translation: AAP31528.1.
L11569 Genomic DNA. Translation: AAA72354.1.
X61934 Genomic DNA. Translation: CAA43938.1.
X61935 Genomic DNA. Translation: CAA43939.1.
PIRiA25344.
UniGeneiRn.214529.

Genome annotation databases

UCSCiRGD:2177. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J02649 mRNA. Translation: AAA66036.1 .
S74801 mRNA. Translation: AAP31528.1 .
L11569 Genomic DNA. Translation: AAA72354.1 .
X61934 Genomic DNA. Translation: CAA43938.1 .
X61935 Genomic DNA. Translation: CAA43939.1 .
PIRi A25344.
UniGenei Rn.214529.

3D structure databases

ProteinModelPortali P09626.
SMRi P09626. Positions 1-33, 35-1033.
ModBasei Search...
MobiDBi Search...

Chemistry

BindingDBi P09626.
ChEMBLi CHEMBL2095199.

PTM databases

PhosphoSitei P09626.

Proteomic databases

PaxDbi P09626.
PRIDEi P09626.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi RGD:2177. rat.

Organism-specific databases

RGDi 2177. Atp4a.

Phylogenomic databases

eggNOGi COG0474.
HOGENOMi HOG000265622.
HOVERGENi HBG004298.
InParanoidi P09626.
PhylomeDBi P09626.

Enzyme and pathway databases

BRENDAi 3.6.3.10. 5301.

Gene expression databases

Genevestigatori P09626.

Family and domain databases

Gene3Di 1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProi IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR015127. ATPase_P-typ_H/K-transp_N.
IPR005775. ATPase_P-typ_Na/K_IIC.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view ]
Pfami PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF09040. H-K_ATPase_N. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view ]
PRINTSi PR00119. CATATPASE.
SMARTi SM00831. Cation_ATPase_N. 1 hit.
[Graphical view ]
SUPFAMi SSF56784. SSF56784. 3 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsi TIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning of the rat stomach (H+ + K+)-ATPase."
    Shull G.E., Lingrel J.B.
    J. Biol. Chem. 261:16788-16791(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Shull G.E.
    Submitted (FEB-1987) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  3. "Expression and cellular localization of mRNA encoding the 'gastric' isoform of H(+)-K(+)-ATPase alpha-subunit in rat kidney."
    Ahn K.Y., Kone B.C.
    Am. J. Physiol. 268:F99-F109(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 54-507.
  4. Song I., Mortell P., Gantz I., Marino L.R., Yamada T.
    Submitted (FEB-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-50.
    Tissue: Liver.
  5. "Control region and gastric specific transcription of the rat H+,K(+)-ATPase alpha subunit gene."
    Oshiman K., Motajima K., Mahmood S., Shimada A., Tamura S., Maeda M., Futai M.
    FEBS Lett. 281:250-254(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 177-260 AND 436-466.
    Strain: Sprague-Dawley.

Entry informationi

Entry nameiATP4A_RAT
AccessioniPrimary (citable) accession number: P09626
Secondary accession number(s): P70511
, P97892, Q63253, Q6LD86
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 23, 2007
Last modified: October 1, 2014
This is version 128 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3