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P09626

- ATP4A_RAT

UniProt

P09626 - ATP4A_RAT

Protein

Potassium-transporting ATPase alpha chain 1

Gene

Atp4a

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 128 (01 Oct 2014)
      Sequence version 3 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Catalyzes the hydrolysis of ATP coupled with the exchange of H+ and K+ ions across the plasma membrane. Responsible for acid production in the stomach.

    Catalytic activityi

    ATP + H2O + H+(In) + K+(Out) = ADP + phosphate + H+(Out) + K+(In).

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei385 – 38514-aspartylphosphate intermediateBy similarity
    Metal bindingi726 – 7261MagnesiumBy similarity
    Metal bindingi730 – 7301MagnesiumBy similarity

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. hydrogen:potassium-exchanging ATPase activity Source: RGD
    3. magnesium ion binding Source: InterPro

    GO - Biological processi

    1. ATP biosynthetic process Source: InterPro
    2. ATP hydrolysis coupled proton transport Source: InterPro
    3. gastric acid secretion Source: RGD

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Hydrogen ion transport, Ion transport, Potassium transport, Transport

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium

    Enzyme and pathway databases

    BRENDAi3.6.3.10. 5301.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Potassium-transporting ATPase alpha chain 1 (EC:3.6.3.10)
    Alternative name(s):
    Gastric H(+)/K(+) ATPase subunit alpha
    Proton pump
    Gene namesi
    Name:Atp4a
    Synonyms:Hka
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi2177. Atp4a.

    Subcellular locationi

    GO - Cellular componenti

    1. apical plasma membrane Source: UniProtKB
    2. integral component of membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 10331032Potassium-transporting ATPase alpha chain 1PRO_0000046257Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei6 – 61PhosphotyrosineBy similarity
    Modified residuei9 – 91PhosphotyrosineBy similarity
    Modified residuei26 – 261PhosphoserineBy similarity
    Modified residuei952 – 9521Phosphoserine; by PKABy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    PaxDbiP09626.
    PRIDEiP09626.

    PTM databases

    PhosphoSiteiP09626.

    Expressioni

    Gene expression databases

    GenevestigatoriP09626.

    Interactioni

    Subunit structurei

    Composed of two subunits: alpha (catalytic) and beta.

    Structurei

    3D structure databases

    ProteinModelPortaliP09626.
    SMRiP09626. Positions 1-33, 35-1033.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini2 – 9695CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini118 – 14023LumenalSequence AnalysisAdd
    BLAST
    Topological domaini162 – 297136CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini318 – 32912LumenalSequence AnalysisAdd
    BLAST
    Topological domaini348 – 781434CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini802 – 81110LumenalSequence Analysis
    Topological domaini833 – 85220CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini876 – 92752LumenalSequence AnalysisAdd
    BLAST
    Topological domaini948 – 96114CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini981 – 99515LumenalSequence AnalysisAdd
    BLAST
    Topological domaini1017 – 103317CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei97 – 11721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei141 – 16121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei298 – 31720HelicalSequence AnalysisAdd
    BLAST
    Transmembranei330 – 34718HelicalSequence AnalysisAdd
    BLAST
    Transmembranei782 – 80120HelicalSequence AnalysisAdd
    BLAST
    Transmembranei812 – 83221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei853 – 87523HelicalSequence AnalysisAdd
    BLAST
    Transmembranei928 – 94720HelicalSequence AnalysisAdd
    BLAST
    Transmembranei962 – 98019HelicalSequence AnalysisAdd
    BLAST
    Transmembranei996 – 101621HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0474.
    HOGENOMiHOG000265622.
    HOVERGENiHBG004298.
    InParanoidiP09626.
    PhylomeDBiP09626.

    Family and domain databases

    Gene3Di1.20.1110.10. 2 hits.
    2.70.150.10. 2 hits.
    3.40.1110.10. 1 hit.
    InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
    IPR004014. ATPase_P-typ_cation-transptr_N.
    IPR023299. ATPase_P-typ_cyto_domN.
    IPR015127. ATPase_P-typ_H/K-transp_N.
    IPR005775. ATPase_P-typ_Na/K_IIC.
    IPR018303. ATPase_P-typ_P_site.
    IPR023298. ATPase_P-typ_TM_dom.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR023214. HAD-like_dom.
    [Graphical view]
    PfamiPF00689. Cation_ATPase_C. 1 hit.
    PF00690. Cation_ATPase_N. 1 hit.
    PF00122. E1-E2_ATPase. 1 hit.
    PF09040. H-K_ATPase_N. 1 hit.
    PF00702. Hydrolase. 1 hit.
    [Graphical view]
    PRINTSiPR00119. CATATPASE.
    SMARTiSM00831. Cation_ATPase_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF56784. SSF56784. 3 hits.
    SSF81660. SSF81660. 1 hit.
    TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
    TIGR01494. ATPase_P-type. 2 hits.
    PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P09626-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGKENYELYS VELGTGPGGD MAAKMSKKKA GGGGGKKKEK LENMKKEMEM     50
    NDHQLSVSEL EQKYQTSATK GLKASLAAEL LLRDGPNALR PPRGTPEYVK 100
    FARQLAGGLQ CLMWVAAAIC LIAFAIQASE GDLTTDDNLY LALALIAVVV 150
    VTGCFGYYQE FKSTNIIASF KNLVPQQATV IRDGDKFQIN ADQLVVGDLV 200
    EMKGGDRVPA DIRILSAQGC KVDNSSLTGE SEPQTRSPEC THESPLETRN 250
    IAFFSTMCLE GTAQGLVVST GDRTIIGRIA SLASGVENEK TPIAIEIEHF 300
    VDIIAGLAIL FGATFFVVAM CIGYTFLRAM VFFMAIVVAY VPEGLLATVT 350
    VCLSLTAKRL ASKNCVVKNL EAVETLGSTS VICSDKTGTL TQNRMTVSHL 400
    WFDNHIHTAD TTEDQSGQTF DQSSETWRAL CRVLTLCNRA AFKSGQDAVP 450
    VPKRIVIGDA SETALLKFSE LTLGNAMGYR DRFPKVCEIP FNSTNKFQLS 500
    IHTLEDPRDP RHLLVMKGAP ERVLERCSSI LIKGQELPLD EQWREAFQTA 550
    YLSLGGLGER VLGFCQLYLN EKDYPPGYTF DVEAMNFPSS GLCFAGLVSM 600
    IDPPRATVPD AVLKCRTAGI RVIMVTGDHP ITAKAIAASV GIISEGSETV 650
    EDIAARLRMP VDQVNKKDAR ACVINGMQLK DMDPSELVEA LRTHPEMVFA 700
    RTSPQQKLVI VESCQRLGAI VAVTGDGVND SPALKKADIG VAMGIAGSDA 750
    AKNAADMILL DDNFASIVTG VEQGRLIFDN LKKSIAYTLT KNIPELTPYL 800
    IYITVSVPLP LGCITILFIE LCTDIFPSVS LAYEKAESDI MHLRPRNPRR 850
    DRLVNEPLAA YSYFQIGAIQ SFAGFADYFT AMAQEGWFPL LCVGLRPQWE 900
    DHHLQDLQDS YGQEWTFGQR LYQQYTCYTV FFISIEMCQI ADVLIRKTRR 950
    LSAFQQGFFR NRILVIAIVF QVCIGCFLCY CPGMPNIFNF MPIRFQWWLV 1000
    PMPFGLLIFV YDEIRKLGVR CCPGSWWDQE LYY 1033
    Length:1,033
    Mass (Da):114,038
    Last modified:January 23, 2007 - v3
    Checksum:i5A8835BA0CAF987E
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti3 – 31K → KA in AAA72354. 1 PublicationCurated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02649 mRNA. Translation: AAA66036.1.
    S74801 mRNA. Translation: AAP31528.1.
    L11569 Genomic DNA. Translation: AAA72354.1.
    X61934 Genomic DNA. Translation: CAA43938.1.
    X61935 Genomic DNA. Translation: CAA43939.1.
    PIRiA25344.
    UniGeneiRn.214529.

    Genome annotation databases

    UCSCiRGD:2177. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    J02649 mRNA. Translation: AAA66036.1 .
    S74801 mRNA. Translation: AAP31528.1 .
    L11569 Genomic DNA. Translation: AAA72354.1 .
    X61934 Genomic DNA. Translation: CAA43938.1 .
    X61935 Genomic DNA. Translation: CAA43939.1 .
    PIRi A25344.
    UniGenei Rn.214529.

    3D structure databases

    ProteinModelPortali P09626.
    SMRi P09626. Positions 1-33, 35-1033.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi P09626.
    ChEMBLi CHEMBL2095199.

    PTM databases

    PhosphoSitei P09626.

    Proteomic databases

    PaxDbi P09626.
    PRIDEi P09626.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    UCSCi RGD:2177. rat.

    Organism-specific databases

    RGDi 2177. Atp4a.

    Phylogenomic databases

    eggNOGi COG0474.
    HOGENOMi HOG000265622.
    HOVERGENi HBG004298.
    InParanoidi P09626.
    PhylomeDBi P09626.

    Enzyme and pathway databases

    BRENDAi 3.6.3.10. 5301.

    Gene expression databases

    Genevestigatori P09626.

    Family and domain databases

    Gene3Di 1.20.1110.10. 2 hits.
    2.70.150.10. 2 hits.
    3.40.1110.10. 1 hit.
    InterProi IPR006068. ATPase_P-typ_cation-transptr_C.
    IPR004014. ATPase_P-typ_cation-transptr_N.
    IPR023299. ATPase_P-typ_cyto_domN.
    IPR015127. ATPase_P-typ_H/K-transp_N.
    IPR005775. ATPase_P-typ_Na/K_IIC.
    IPR018303. ATPase_P-typ_P_site.
    IPR023298. ATPase_P-typ_TM_dom.
    IPR008250. ATPase_P-typ_transduc_dom_A.
    IPR001757. Cation_transp_P_typ_ATPase.
    IPR023214. HAD-like_dom.
    [Graphical view ]
    Pfami PF00689. Cation_ATPase_C. 1 hit.
    PF00690. Cation_ATPase_N. 1 hit.
    PF00122. E1-E2_ATPase. 1 hit.
    PF09040. H-K_ATPase_N. 1 hit.
    PF00702. Hydrolase. 1 hit.
    [Graphical view ]
    PRINTSi PR00119. CATATPASE.
    SMARTi SM00831. Cation_ATPase_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56784. SSF56784. 3 hits.
    SSF81660. SSF81660. 1 hit.
    TIGRFAMsi TIGR01106. ATPase-IIC_X-K. 1 hit.
    TIGR01494. ATPase_P-type. 2 hits.
    PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of the rat stomach (H+ + K+)-ATPase."
      Shull G.E., Lingrel J.B.
      J. Biol. Chem. 261:16788-16791(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Shull G.E.
      Submitted (FEB-1987) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION.
    3. "Expression and cellular localization of mRNA encoding the 'gastric' isoform of H(+)-K(+)-ATPase alpha-subunit in rat kidney."
      Ahn K.Y., Kone B.C.
      Am. J. Physiol. 268:F99-F109(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 54-507.
    4. Song I., Mortell P., Gantz I., Marino L.R., Yamada T.
      Submitted (FEB-1993) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-50.
      Tissue: Liver.
    5. "Control region and gastric specific transcription of the rat H+,K(+)-ATPase alpha subunit gene."
      Oshiman K., Motajima K., Mahmood S., Shimada A., Tamura S., Maeda M., Futai M.
      FEBS Lett. 281:250-254(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 177-260 AND 436-466.
      Strain: Sprague-Dawley.

    Entry informationi

    Entry nameiATP4A_RAT
    AccessioniPrimary (citable) accession number: P09626
    Secondary accession number(s): P70511
    , P97892, Q63253, Q6LD86
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 128 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3