SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P09626

- ATP4A_RAT

UniProt

P09626 - ATP4A_RAT

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Potassium-transporting ATPase alpha chain 1
Gene
Atp4a, Hka
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalyzes the hydrolysis of ATP coupled with the exchange of H+ and K+ ions across the plasma membrane. Responsible for acid production in the stomach.

Catalytic activityi

ATP + H2O + H+(In) + K+(Out) = ADP + phosphate + H+(Out) + K+(In).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei385 – 38514-aspartylphosphate intermediate By similarity
Metal bindingi726 – 7261Magnesium By similarity
Metal bindingi730 – 7301Magnesium By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. hydrogen:potassium-exchanging ATPase activity Source: RGD
  3. magnesium ion binding Source: InterPro
Complete GO annotation...

GO - Biological processi

  1. ATP biosynthetic process Source: InterPro
  2. ATP hydrolysis coupled proton transport Source: InterPro
  3. gastric acid secretion Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Hydrogen ion transport, Ion transport, Potassium transport, Transport

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium

Enzyme and pathway databases

BRENDAi3.6.3.10. 5301.

Names & Taxonomyi

Protein namesi
Recommended name:
Potassium-transporting ATPase alpha chain 1 (EC:3.6.3.10)
Alternative name(s):
Gastric H(+)/K(+) ATPase subunit alpha
Proton pump
Gene namesi
Name:Atp4a
Synonyms:Hka
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Unplaced

Organism-specific databases

RGDi2177. Atp4a.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 9695Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei97 – 11721Helical; Reviewed prediction
Add
BLAST
Topological domaini118 – 14023Lumenal Reviewed prediction
Add
BLAST
Transmembranei141 – 16121Helical; Reviewed prediction
Add
BLAST
Topological domaini162 – 297136Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei298 – 31720Helical; Reviewed prediction
Add
BLAST
Topological domaini318 – 32912Lumenal Reviewed prediction
Add
BLAST
Transmembranei330 – 34718Helical; Reviewed prediction
Add
BLAST
Topological domaini348 – 781434Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei782 – 80120Helical; Reviewed prediction
Add
BLAST
Topological domaini802 – 81110Lumenal Reviewed prediction
Transmembranei812 – 83221Helical; Reviewed prediction
Add
BLAST
Topological domaini833 – 85220Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei853 – 87523Helical; Reviewed prediction
Add
BLAST
Topological domaini876 – 92752Lumenal Reviewed prediction
Add
BLAST
Transmembranei928 – 94720Helical; Reviewed prediction
Add
BLAST
Topological domaini948 – 96114Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei962 – 98019Helical; Reviewed prediction
Add
BLAST
Topological domaini981 – 99515Lumenal Reviewed prediction
Add
BLAST
Transmembranei996 – 101621Helical; Reviewed prediction
Add
BLAST
Topological domaini1017 – 103317Cytoplasmic Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. apical plasma membrane Source: UniProtKB
  2. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 10331032Potassium-transporting ATPase alpha chain 1
PRO_0000046257Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei6 – 61Phosphotyrosine By similarity
Modified residuei9 – 91Phosphotyrosine By similarity
Modified residuei26 – 261Phosphoserine By similarity
Modified residuei952 – 9521Phosphoserine; by PKA By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP09626.
PRIDEiP09626.

PTM databases

PhosphoSiteiP09626.

Expressioni

Gene expression databases

GenevestigatoriP09626.

Interactioni

Subunit structurei

Composed of two subunits: alpha (catalytic) and beta.

Structurei

3D structure databases

ProteinModelPortaliP09626.
SMRiP09626. Positions 1-33, 35-1033.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0474.
HOGENOMiHOG000265622.
HOVERGENiHBG004298.
InParanoidiP09626.
PhylomeDBiP09626.

Family and domain databases

Gene3Di1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProiIPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR015127. ATPase_P-typ_H/K-transp_N.
IPR005775. ATPase_P-typ_Na/K_IIC.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view]
PfamiPF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF09040. H-K_ATPase_N. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view]
PRINTSiPR00119. CATATPASE.
SMARTiSM00831. Cation_ATPase_N. 1 hit.
[Graphical view]
SUPFAMiSSF56784. SSF56784. 3 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsiTIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEiPS00154. ATPASE_E1_E2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09626-1 [UniParc]FASTAAdd to Basket

« Hide

MGKENYELYS VELGTGPGGD MAAKMSKKKA GGGGGKKKEK LENMKKEMEM     50
NDHQLSVSEL EQKYQTSATK GLKASLAAEL LLRDGPNALR PPRGTPEYVK 100
FARQLAGGLQ CLMWVAAAIC LIAFAIQASE GDLTTDDNLY LALALIAVVV 150
VTGCFGYYQE FKSTNIIASF KNLVPQQATV IRDGDKFQIN ADQLVVGDLV 200
EMKGGDRVPA DIRILSAQGC KVDNSSLTGE SEPQTRSPEC THESPLETRN 250
IAFFSTMCLE GTAQGLVVST GDRTIIGRIA SLASGVENEK TPIAIEIEHF 300
VDIIAGLAIL FGATFFVVAM CIGYTFLRAM VFFMAIVVAY VPEGLLATVT 350
VCLSLTAKRL ASKNCVVKNL EAVETLGSTS VICSDKTGTL TQNRMTVSHL 400
WFDNHIHTAD TTEDQSGQTF DQSSETWRAL CRVLTLCNRA AFKSGQDAVP 450
VPKRIVIGDA SETALLKFSE LTLGNAMGYR DRFPKVCEIP FNSTNKFQLS 500
IHTLEDPRDP RHLLVMKGAP ERVLERCSSI LIKGQELPLD EQWREAFQTA 550
YLSLGGLGER VLGFCQLYLN EKDYPPGYTF DVEAMNFPSS GLCFAGLVSM 600
IDPPRATVPD AVLKCRTAGI RVIMVTGDHP ITAKAIAASV GIISEGSETV 650
EDIAARLRMP VDQVNKKDAR ACVINGMQLK DMDPSELVEA LRTHPEMVFA 700
RTSPQQKLVI VESCQRLGAI VAVTGDGVND SPALKKADIG VAMGIAGSDA 750
AKNAADMILL DDNFASIVTG VEQGRLIFDN LKKSIAYTLT KNIPELTPYL 800
IYITVSVPLP LGCITILFIE LCTDIFPSVS LAYEKAESDI MHLRPRNPRR 850
DRLVNEPLAA YSYFQIGAIQ SFAGFADYFT AMAQEGWFPL LCVGLRPQWE 900
DHHLQDLQDS YGQEWTFGQR LYQQYTCYTV FFISIEMCQI ADVLIRKTRR 950
LSAFQQGFFR NRILVIAIVF QVCIGCFLCY CPGMPNIFNF MPIRFQWWLV 1000
PMPFGLLIFV YDEIRKLGVR CCPGSWWDQE LYY 1033
Length:1,033
Mass (Da):114,038
Last modified:January 23, 2007 - v3
Checksum:i5A8835BA0CAF987E
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti3 – 31K → KA in AAA72354. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J02649 mRNA. Translation: AAA66036.1.
S74801 mRNA. Translation: AAP31528.1.
L11569 Genomic DNA. Translation: AAA72354.1.
X61934 Genomic DNA. Translation: CAA43938.1.
X61935 Genomic DNA. Translation: CAA43939.1.
PIRiA25344.
UniGeneiRn.214529.

Genome annotation databases

UCSCiRGD:2177. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
J02649 mRNA. Translation: AAA66036.1 .
S74801 mRNA. Translation: AAP31528.1 .
L11569 Genomic DNA. Translation: AAA72354.1 .
X61934 Genomic DNA. Translation: CAA43938.1 .
X61935 Genomic DNA. Translation: CAA43939.1 .
PIRi A25344.
UniGenei Rn.214529.

3D structure databases

ProteinModelPortali P09626.
SMRi P09626. Positions 1-33, 35-1033.
ModBasei Search...

Chemistry

BindingDBi P09626.
ChEMBLi CHEMBL2095199.

PTM databases

PhosphoSitei P09626.

Proteomic databases

PaxDbi P09626.
PRIDEi P09626.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

UCSCi RGD:2177. rat.

Organism-specific databases

RGDi 2177. Atp4a.

Phylogenomic databases

eggNOGi COG0474.
HOGENOMi HOG000265622.
HOVERGENi HBG004298.
InParanoidi P09626.
PhylomeDBi P09626.

Enzyme and pathway databases

BRENDAi 3.6.3.10. 5301.

Gene expression databases

Genevestigatori P09626.

Family and domain databases

Gene3Di 1.20.1110.10. 2 hits.
2.70.150.10. 2 hits.
3.40.1110.10. 1 hit.
InterProi IPR006068. ATPase_P-typ_cation-transptr_C.
IPR004014. ATPase_P-typ_cation-transptr_N.
IPR023299. ATPase_P-typ_cyto_domN.
IPR015127. ATPase_P-typ_H/K-transp_N.
IPR005775. ATPase_P-typ_Na/K_IIC.
IPR018303. ATPase_P-typ_P_site.
IPR023298. ATPase_P-typ_TM_dom.
IPR008250. ATPase_P-typ_transduc_dom_A.
IPR001757. Cation_transp_P_typ_ATPase.
IPR023214. HAD-like_dom.
[Graphical view ]
Pfami PF00689. Cation_ATPase_C. 1 hit.
PF00690. Cation_ATPase_N. 1 hit.
PF00122. E1-E2_ATPase. 1 hit.
PF09040. H-K_ATPase_N. 1 hit.
PF00702. Hydrolase. 1 hit.
[Graphical view ]
PRINTSi PR00119. CATATPASE.
SMARTi SM00831. Cation_ATPase_N. 1 hit.
[Graphical view ]
SUPFAMi SSF56784. SSF56784. 3 hits.
SSF81660. SSF81660. 1 hit.
TIGRFAMsi TIGR01106. ATPase-IIC_X-K. 1 hit.
TIGR01494. ATPase_P-type. 2 hits.
PROSITEi PS00154. ATPASE_E1_E2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning of the rat stomach (H+ + K+)-ATPase."
    Shull G.E., Lingrel J.B.
    J. Biol. Chem. 261:16788-16791(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Shull G.E.
    Submitted (FEB-1987) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION.
  3. "Expression and cellular localization of mRNA encoding the 'gastric' isoform of H(+)-K(+)-ATPase alpha-subunit in rat kidney."
    Ahn K.Y., Kone B.C.
    Am. J. Physiol. 268:F99-F109(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 54-507.
  4. Song I., Mortell P., Gantz I., Marino L.R., Yamada T.
    Submitted (FEB-1993) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-50.
    Tissue: Liver.
  5. "Control region and gastric specific transcription of the rat H+,K(+)-ATPase alpha subunit gene."
    Oshiman K., Motajima K., Mahmood S., Shimada A., Tamura S., Maeda M., Futai M.
    FEBS Lett. 281:250-254(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 177-260 AND 436-466.
    Strain: Sprague-Dawley.

Entry informationi

Entry nameiATP4A_RAT
AccessioniPrimary (citable) accession number: P09626
Secondary accession number(s): P70511
, P97892, Q63253, Q6LD86
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 23, 2007
Last modified: April 16, 2014
This is version 127 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi