Reviewed, UniProtKB/Swiss-Prot P09621 (CH10_MYCTU)

Names and origin

Protein names

Recommended name:
    10 kDa chaperonin
Alternative name(s):
    Protein Cpn10
    groES protein
    BCG-A heat shock protein
    10 kDa antigen

Gene names

Name:	groS
Synonyms:	cpn10, groES, mopB
Ordered Locus Names:	Rv3418c, MT3527
ORF Names:	MTCY78.11

Organism

Mycobacterium tuberculosis [Complete proteome] [HAMAP]

Taxonomic identifier

1773 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Mycobacteriaceae › Mycobacterium › Mycobacterium tuberculosis complex

Protein attributes

Sequence length	100 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Binds to Cpn60 in the presence of Mg-ATP and suppresses the ATPase activity of the latter. HAMAP MF_00580
Subunit structure	Heptamer of 7 subunits arranged in a ring By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the groES chaperonin family.

Ontologies

Keywords
Biological process	Stress response
Cellular component	Cytoplasm
Molecular function	Chaperone
Technical term	3D-structure Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	protein folding Inferred from electronic annotation. Source: HAMAP response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed Ref.7

Chain

2 – 100

99

10 kDa chaperonin HAMAP MF_00580

PRO_0000174788

Secondary structure

1

.

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.

.

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.

.

.

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.

.

.

.

100

Helix Strand Turn

Details...

Beta strand

5 – 8

4

Beta strand

12 – 17

6

Beta strand

37 – 46

10

Beta strand

53 – 56

4

Beta strand

68 – 72

5

Beta strand

77 – 81

5

Beta strand

84 – 90

7

Helix

91 – 93

3

Beta strand

94 – 99

6

Sequences

	Sequence		Length	Mass (Da)
	P09621-1 [UniParc]. Last modified January 23, 2007. Version 3. Checksum: DE448187A56103FE	FASTA	100	10,804
	10 20 30 40 50 60 MAKVNIKPLE DKILVQANEA ETTTASGLVI PDTAKEKPQE GTVVAVGPGR WDEDGEKRIP 70 80 90 100 LDVAEGDTVI YSKYGGTEIK YNGEEYLILS ARDVLAVVSK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A major antigen from Mycobacterium tuberculosis which is homologous to the heat shock proteins groES from E. coli and the htpA gene product of Coxiella burneti." Baird P.N., Hall L.M., Coates A.R.M. Nucleic Acids Res. 16:9047-9047(1988) [PubMed: 2902558] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[2]	"Cloning and sequence analysis of the 10 kDa antigen gene of Mycobacterium tuberculosis." Baird P.N., Hall L.M.C., Coates A.R.M. J. Gen. Microbiol. 135:931-939(1989) [PubMed: 2480990] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[3]	"The Mycobacterium tuberculosis BCG-a protein has homology with the Escherichia coli GroES protein." Shinnick T.M., Plikaytis B.P., Hyche A.D., van Landingham R.M., Walker L.L. Nucleic Acids Res. 17:1254-1254(1989) [PubMed: 2564178] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 35801 / TMC 107 / Erdman.
[4]	"Mycobacterium tuberculosis expresses two chaperonin-60 homologs." Kong T.H., Coates A.R.M., Butcher P.D., Hickman C.J., Shinnick T.M. Proc. Natl. Acad. Sci. U.S.A. 90:2608-2612(1993) [PubMed: 7681982] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 35801 / TMC 107 / Erdman.
[5]	"Deciphering the biology of Mycobacterium tuberculosis from the complete genome sequence." Cole S.T., Brosch R., Parkhill J., Garnier T., Churcher C.M., Harris D.E., Gordon S.V., Eiglmeier K., Gas S., Barry C.E. III, Tekaia F., Badcock K., Basham D., Brown D., Chillingworth T., Connor R., Davies R.M., Devlin K. , Feltwell T., Gentles S., Hamlin N., Holroyd S., Hornsby T., Jagels K., Krogh A., McLean J., Moule S., Murphy L.D., Oliver S., Osborne J., Quail M.A., Rajandream M.A., Rogers J., Rutter S., Seeger K., Skelton S., Squares S., Squares R., Sulston J.E., Taylor K., Whitehead S., Barrell B.G. Nature 393:537-544(1998) [PubMed: 9634230] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 25618 / H37Rv.
[6]	"Whole-genome comparison of Mycobacterium tuberculosis clinical and laboratory strains." Fleischmann R.D., Alland D., Eisen J.A., Carpenter L., White O., Peterson J.D., DeBoy R.T., Dodson R.J., Gwinn M.L., Haft D.H., Hickey E.K., Kolonay J.F., Nelson W.C., Umayam L.A., Ermolaeva M.D., Salzberg S.L., Delcher A., Utterback T.R. , Weidman J.F., Khouri H.M., Gill J., Mikula A., Bishai W., Jacobs W.R. Jr., Venter J.C., Fraser C.M. J. Bacteriol. 184:5479-5490(2002) [PubMed: 12218036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CDC 1551 / Oshkosh.
[7]	"Immunoreactivity of a 10-kDa antigen of Mycobacterium tuberculosis." Barnes P.F., Mehra V., Rivoire B., Fong S.J., Brennan P.J., Voegtline M.S., Minden P., Houghten R.A., Bloom B.R., Modlin R.L. J. Immunol. 148:1835-1840(1992) [PubMed: 1371791] [Abstract] Cited for: PROTEIN SEQUENCE OF 2-16.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBLGenBankDDBJ

X60350 Genomic DNA. Translation: CAA42908.1.
M25258 Genomic DNA. Translation: AAA25340.1.
X13739 Genomic DNA. Translation: CAA32003.1.
BX842583 Genomic DNA. Translation: CAB01005.1.
AE000516 Genomic DNA. Translation: AAK47865.1.

PIR

BVMYBA. S01381.

RefSeq

NP_217935.1.
NP_338051.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1HX5	X-ray	3.50	A/B/C/D/E/F/G	2-99	[»]
1P3H	X-ray	2.80	A/B/C/D/E/F/G/H/I/J/K/L/M/N	2-99	[»]
1P83	NMR	-	A	2-25	[»]

ModBase

Search...

Genome annotation databases

GeneID

887583.
923162.

GenomeReviews

Gene locus MT3527 in contig AE000516_GR.
Gene locus Rv3418c in contig AL123456_GR.

KEGG

mtc:MT3527.
mtu:Rv3418c.

TIGR

MT3527.

Organism-specific databases

TubercuList

Rv3418c.

Phylogenomic databases

HOGENOM

P09621.

OMA

YSGTEVS.

Family and domain databases

HAMAP

MF_00580.
[Tree]

InterPro

IPR001476. Chaprnonin_Cpn10.
IPR018369. Chaprnonin_Cpn10_CS.
[Graphical view]

Gene3D

G3DSA:2.30.33.40. Chaprnin_Cpn10. 1 hit.

PANTHER

PTHR10772. Chaprnin_Cpn10. 1 hit.

Pfam

PF00166. Cpn10. 1 hit.
[Graphical view]

PRINTS

PR00297. CHAPERONIN10.

ProDom

PD000566. Chaprnin_Cpn10. 1 hit.
[Graphical view] [Entries sharing at least one domain]

PROSITE

PS00681. CHAPERONINS_CPN10. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

CH10_MYCTU

Accession

Primary (citable) accession number: P09621

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	January 23, 2007
Last modified:	November 3, 2009
This is version 95 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)