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Protein

Pheromone-processing carboxypeptidase KEX1

Gene

KEX1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Protease with a carboxypeptidase B-like function involved in the C-terminal processing of the lysine and arginine residues from the precursors of K1, K2 and K28 killer toxins and a-factor (mating pheromone). Involved in the programmed cell death caused by defective N-glycosylation and contributes also to the active cell death program induced by acetic acid stress or during chronological aging. Promotes cell fusion by proteolytically processing substrates that act in parallel to PRM1 as an alternative fusion machine, as cell wall components, or both.11 Publications

Catalytic activityi

Preferential release of a C-terminal arginine or lysine residue.

Kineticsi

  1. KM=284 µM for benzoyl-Phe-Ala-Arg1 Publication
  2. KM=516 µM for furylacryloyl-Ala-Arg1 Publication
  3. KM=962 µM for furylacryloyl-Ala-Lys1 Publication
  1. Vmax=64.25 µmol/min/mg enzyme toward benzoyl-Phe-Ala-Arg1 Publication
  2. Vmax=22.35 µmol/min/mg enzyme toward furylacryloyl-Ala-Arg1 Publication
  3. Vmax=11.55 µmol/min/mg enzyme toward furylacryloyl-Ala-Lys1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei1981
Active sitei405By similarity1
Active sitei470By similarity1

GO - Molecular functioni

  • serine-type carboxypeptidase activity Source: SGD

GO - Biological processi

  • apoptotic process Source: SGD
  • proteolysis involved in cellular protein catabolic process Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Carboxypeptidase, Hydrolase, Protease

Keywords - Biological processi

Apoptosis

Enzyme and pathway databases

BioCyciMetaCyc:YGL203C-MONOMER.
YEAST:YGL203C-MONOMER.
BRENDAi3.4.16.6. 984.

Protein family/group databases

ESTHERiyeast-kex01. Carboxypeptidase_S10.
MEROPSiS10.007.

Names & Taxonomyi

Protein namesi
Recommended name:
Pheromone-processing carboxypeptidase KEX1 (EC:3.4.16.6)
Alternative name(s):
Carboxypeptidase D
Killer expression defective protein 1
Gene namesi
Name:KEX1
Ordered Locus Names:YGL203C
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGL203C.
SGDiS000003171. KEX1.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini23 – 616LumenalSequence analysisAdd BLAST594
Transmembranei617 – 637HelicalSequence analysisAdd BLAST21
Topological domaini638 – 729CytoplasmicSequence analysisAdd BLAST92

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • trans-Golgi network Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Golgi apparatus, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi198S → A: Inactivates enzyme. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 22Add BLAST22
ChainiPRO_000000428723 – 729Pheromone-processing carboxypeptidase KEX1Add BLAST707

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi81N-linked (GlcNAc...)Sequence analysis1
Glycosylationi459N-linked (GlcNAc...)1 Publication1
Glycosylationi467N-linked (GlcNAc...)1 Publication1
Modified residuei660PhosphoserineCombined sources1

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP09620.
PRIDEiP09620.

PTM databases

iPTMnetiP09620.

Interactioni

Protein-protein interaction databases

BioGridi33053. 101 interactors.
IntActiP09620. 57 interactors.
MINTiMINT-2780061.

Structurei

Secondary structure

1729
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi26 – 28Combined sources3
Helixi33 – 35Combined sources3
Helixi39 – 41Combined sources3
Beta strandi50 – 57Combined sources8
Beta strandi61 – 63Combined sources3
Beta strandi72 – 79Combined sources8
Helixi84 – 86Combined sources3
Beta strandi91 – 95Combined sources5
Turni98 – 100Combined sources3
Helixi104 – 109Combined sources6
Beta strandi110 – 116Combined sources7
Beta strandi122 – 124Combined sources3
Helixi129 – 131Combined sources3
Beta strandi133 – 138Combined sources6
Helixi154 – 156Combined sources3
Helixi166 – 183Combined sources18
Helixi187 – 189Combined sources3
Beta strandi190 – 198Combined sources9
Helixi200 – 217Combined sources18
Beta strandi226 – 235Combined sources10
Helixi239 – 243Combined sources5
Helixi246 – 252Combined sources7
Helixi263 – 279Combined sources17
Helixi283 – 286Combined sources4
Beta strandi287 – 289Combined sources3
Helixi291 – 294Combined sources4
Helixi296 – 303Combined sources8
Beta strandi314 – 317Combined sources4
Beta strandi320 – 325Combined sources6
Turni327 – 333Combined sources7
Helixi337 – 345Combined sources9
Helixi350 – 353Combined sources4
Turni358 – 360Combined sources3
Helixi369 – 374Combined sources6
Helixi383 – 386Combined sources4
Helixi387 – 392Combined sources6
Beta strandi396 – 402Combined sources7
Helixi410 – 419Combined sources10
Beta strandi425 – 428Combined sources4
Beta strandi433 – 439Combined sources7
Beta strandi452 – 457Combined sources6
Beta strandi460 – 465Combined sources6
Helixi472 – 475Combined sources4
Helixi477 – 487Combined sources11
Beta strandi492 – 496Combined sources5
Beta strandi499 – 504Combined sources6

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AC5X-ray2.40A23-505[»]
ProteinModelPortaliP09620.
SMRiP09620.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09620.

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi526 – 531Poly-Glu6
Compositional biasi545 – 561Poly-AspAdd BLAST17
Compositional biasi567 – 578Poly-AspAdd BLAST12
Compositional biasi597 – 607Poly-GluAdd BLAST11
Compositional biasi703 – 711Poly-Lys9

Sequence similaritiesi

Belongs to the peptidase S10 family.Curated

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

GeneTreeiENSGT00860000133853.
HOGENOMiHOG000113220.
InParanoidiP09620.
KOiK01288.
OMAiQECENIL.
OrthoDBiEOG092C255X.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR001563. Peptidase_S10.
IPR033124. Ser_caboxypep_his_AS.
IPR018202. Ser_caboxypep_ser_AS.
[Graphical view]
PANTHERiPTHR11802. PTHR11802. 1 hit.
PfamiPF00450. Peptidase_S10. 1 hit.
[Graphical view]
PRINTSiPR00724. CRBOXYPTASEC.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00560. CARBOXYPEPT_SER_HIS. 1 hit.
PS00131. CARBOXYPEPT_SER_SER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09620-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFYNRWLGTW LAMSALIRIS VSLPSSEEYK VAYELLPGLS EVPDPSNIPQ
60 70 80 90 100
MHAGHIPLRS EDADEQDSSD LEYFFWKFTN NDSNGNVDRP LIIWLNGGPG
110 120 130 140 150
CSSMDGALVE SGPFRVNSDG KLYLNEGSWI SKGDLLFIDQ PTGTGFSVEQ
160 170 180 190 200
NKDEGKIDKN KFDEDLEDVT KHFMDFLENY FKIFPEDLTR KIILSGESYA
210 220 230 240 250
GQYIPFFANA ILNHNKFSKI DGDTYDLKAL LIGNGWIDPN TQSLSYLPFA
260 270 280 290 300
MEKKLIDESN PNFKHLTNAH ENCQNLINSA STDEAAHFSY QECENILNLL
310 320 330 340 350
LSYTRESSQK GTADCLNMYN FNLKDSYPSC GMNWPKDISF VSKFFSTPGV
360 370 380 390 400
IDSLHLDSDK IDHWKECTNS VGTKLSNPIS KPSIHLLPGL LESGIEIVLF
410 420 430 440 450
NGDKDLICNN KGVLDTIDNL KWGGIKGFSD DAVSFDWIHK SKSTDDSEEF
460 470 480 490 500
SGYVKYDRNL TFVSVYNASH MVPFDKSLVS RGIVDIYSND VMIIDNNGKN
510 520 530 540 550
VMITTDDDSD QDATTESGDK PKENLEEEEQ EAQNEEGKEK EGNKDKDGDD
560 570 580 590 600
DNDNDDDDED DHNSEGDDDD DDDDDEDDNN EKQSNQGLED SRHKSSEYEQ
610 620 630 640 650
EEEEVEEFAE EISMYKHKAV VVTIVTFLIV VLGVYAYDRR VRRKARHTIL
660 670 680 690 700
VDPNNRQHDS PNKTVSWADD LESGLGAEDD LEQDEQLEGG APISSTSNKA
710 720
GSKLKTKKKK KYTSLPNTEI DESFEMTDF
Length:729
Mass (Da):82,245
Last modified:July 1, 1989 - v1
Checksum:i70583F279AC02A41
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17231 Genomic DNA. Translation: AAA34717.1.
Z72725 Genomic DNA. Translation: CAA96915.1.
BK006941 Genomic DNA. Translation: DAA07912.1.
PIRiA29651.
RefSeqiNP_011312.1. NM_001181068.1.

Genome annotation databases

EnsemblFungiiYGL203C; YGL203C; YGL203C.
GeneIDi852670.
KEGGisce:YGL203C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M17231 Genomic DNA. Translation: AAA34717.1.
Z72725 Genomic DNA. Translation: CAA96915.1.
BK006941 Genomic DNA. Translation: DAA07912.1.
PIRiA29651.
RefSeqiNP_011312.1. NM_001181068.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AC5X-ray2.40A23-505[»]
ProteinModelPortaliP09620.
SMRiP09620.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33053. 101 interactors.
IntActiP09620. 57 interactors.
MINTiMINT-2780061.

Protein family/group databases

ESTHERiyeast-kex01. Carboxypeptidase_S10.
MEROPSiS10.007.

PTM databases

iPTMnetiP09620.

Proteomic databases

MaxQBiP09620.
PRIDEiP09620.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGL203C; YGL203C; YGL203C.
GeneIDi852670.
KEGGisce:YGL203C.

Organism-specific databases

EuPathDBiFungiDB:YGL203C.
SGDiS000003171. KEX1.

Phylogenomic databases

GeneTreeiENSGT00860000133853.
HOGENOMiHOG000113220.
InParanoidiP09620.
KOiK01288.
OMAiQECENIL.
OrthoDBiEOG092C255X.

Enzyme and pathway databases

BioCyciMetaCyc:YGL203C-MONOMER.
YEAST:YGL203C-MONOMER.
BRENDAi3.4.16.6. 984.

Miscellaneous databases

EvolutionaryTraceiP09620.
PROiP09620.

Family and domain databases

Gene3Di3.40.50.1820. 1 hit.
InterProiIPR029058. AB_hydrolase.
IPR001563. Peptidase_S10.
IPR033124. Ser_caboxypep_his_AS.
IPR018202. Ser_caboxypep_ser_AS.
[Graphical view]
PANTHERiPTHR11802. PTHR11802. 1 hit.
PfamiPF00450. Peptidase_S10. 1 hit.
[Graphical view]
PRINTSiPR00724. CRBOXYPTASEC.
SUPFAMiSSF53474. SSF53474. 1 hit.
PROSITEiPS00560. CARBOXYPEPT_SER_HIS. 1 hit.
PS00131. CARBOXYPEPT_SER_SER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKEX1_YEAST
AccessioniPrimary (citable) accession number: P09620
Secondary accession number(s): D6VTV1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: November 30, 2016
This is version 158 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 8550 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.