##gff-version 3
P09616	UniProtKB	Signal peptide	1	26	.	.	.	Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:6500704;Dbxref=PMID:6500704	
P09616	UniProtKB	Chain	27	319	.	.	.	ID=PRO_0000035636;Note=Alpha-hemolysin	
P09616	UniProtKB	Mutagenesis	28	65	.	.	.	Note=Binds host red blood cells%2C complete loss of hemolytic activity%2C no longer oligomerizes. Missing;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:1400487;Dbxref=PMID:1400487	
P09616	UniProtKB	Mutagenesis	28	49	.	.	.	Note=Binds host red blood cells%2C complete loss of hemolytic activity%2C forms hexamers. Missing;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:1400487;Dbxref=PMID:1400487	
P09616	UniProtKB	Mutagenesis	28	39	.	.	.	Note=Binds host red blood cells%2C complete loss of hemolytic activity%2C mainly forms pentamers. Missing;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:1400487;Dbxref=PMID:1400487	
P09616	UniProtKB	Mutagenesis	28	29	.	.	.	Note=Binds host red blood cells%2C complete loss of hemolytic activity%2C mainly forms pentamers. Missing;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:1400487;Dbxref=PMID:1400487	
P09616	UniProtKB	Mutagenesis	61	61	.	.	.	Note=No oligomerization nor hemolytic activity%2C wild-type target cell-binding. H->I%2CL%2CP%2CR%2CS%2CT;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:8188346;Dbxref=PMID:8188346	
P09616	UniProtKB	Mutagenesis	61	61	.	.	.	Note=No hemolytic activity%2C reduced oligomerization%2C not toxic in mice%2C no effect on ADAM10 binding. H->L;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:20624979,ECO:0000269|PubMed:8168947;Dbxref=PMID:20624979,PMID:8168947	
P09616	UniProtKB	Mutagenesis	74	74	.	.	.	Note=No oligomerization%2C altered hemolysis%2C near wild-type target cell binding. H->I%2CR%2CT;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:8188346;Dbxref=PMID:8188346	
P09616	UniProtKB	Mutagenesis	74	74	.	.	.	Note=7%25 of normal hemolytic activity%2C reduced toxicity in mice. H->L;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:8168947;Dbxref=PMID:8168947	
P09616	UniProtKB	Mutagenesis	170	170	.	.	.	Note=Decreased hemolysis%2C wild-type oligomerization and target cell binding. H->L%2CP%2CR;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:8188346;Dbxref=PMID:8188346	
P09616	UniProtKB	Mutagenesis	170	170	.	.	.	Note=16%25 of normal hemolytic activity. H->L;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:8168947;Dbxref=PMID:8168947	
P09616	UniProtKB	Mutagenesis	285	285	.	.	.	Note=Nearly wild-type oligomerization%2C hemolysis and target cell binding. H->I%2CP%2CR%2CS;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:8188346;Dbxref=PMID:8188346	
P09616	UniProtKB	Mutagenesis	285	285	.	.	.	Note=46%25 of normal hemolytic activity%2C slowly toxic in mice. H->L;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:8168947;Dbxref=PMID:8168947	
P09616	UniProtKB	Mutagenesis	312	319	.	.	.	Note=Binds host red blood cells%2C complete loss of hemolytic activity%2C no longer oligomerizes. Missing;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:1400487;Dbxref=PMID:1400487	
P09616	UniProtKB	Mutagenesis	315	319	.	.	.	Note=Binds host red blood cells%2C almost complete loss of hemolytic activity%2C greatly reduced oligomerization. Missing;Ontology_term=ECO:0000269,ECO:0000269;evidence=ECO:0000269|PubMed:1400487,ECO:0000269|PubMed:1587866;Dbxref=PMID:1400487,PMID:1587866	
P09616	UniProtKB	Mutagenesis	317	319	.	.	.	Note=Binds host red blood cells%2C almost complete loss of hemolytic activity%2C greatly reduced oligomerization. Missing;Ontology_term=ECO:0000269;evidence=ECO:0000269|PubMed:1400487;Dbxref=PMID:1400487	
P09616	UniProtKB	Helix	28	31	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7AHL	
P09616	UniProtKB	Turn	35	38	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7AHL	
P09616	UniProtKB	Beta strand	40	43	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:4IDJ	
P09616	UniProtKB	Beta strand	47	55	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7AHL	
P09616	UniProtKB	Turn	56	59	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7AHL	
P09616	UniProtKB	Beta strand	60	69	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7AHL	
P09616	UniProtKB	Beta strand	74	87	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7AHL	
P09616	UniProtKB	Beta strand	92	95	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:3M4D	
P09616	UniProtKB	Turn	98	100	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:6U3T	
P09616	UniProtKB	Beta strand	101	115	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7AHL	
P09616	UniProtKB	Beta strand	123	129	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7AHL	
P09616	UniProtKB	Beta strand	135	153	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7AHL	
P09616	UniProtKB	Beta strand	158	184	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7AHL	
P09616	UniProtKB	Beta strand	188	197	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7AHL	
P09616	UniProtKB	Beta strand	200	202	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7AHL	
P09616	UniProtKB	Beta strand	205	208	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7AHL	
P09616	UniProtKB	Turn	215	217	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7AHL	
P09616	UniProtKB	Helix	232	234	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7AHL	
P09616	UniProtKB	Helix	239	241	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7AHL	
P09616	UniProtKB	Helix	244	247	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7AHL	
P09616	UniProtKB	Beta strand	254	260	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7AHL	
P09616	UniProtKB	Beta strand	268	286	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7AHL	
P09616	UniProtKB	Beta strand	288	311	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7AHL	
P09616	UniProtKB	Turn	312	315	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7AHL	
P09616	UniProtKB	Beta strand	316	318	.	.	.	Ontology_term=ECO:0007829;evidence=ECO:0007829|PDB:7AHL