P09612 (GP_BUNL7) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Envelope glycoprotein
Short name=GP

Alternative name(s):
M polyprotein

Cleaved into the following 3 chains:

1. Glycoprotein G2
   Short name=Gn
2. Non-structural protein NSm
3. Glycoprotein G1
   Short name=Gc

Gene names

Name:

GP

Organism

Bunyavirus La Crosse (isolate Aedes triseriatus/United States/L74/1974)

Taxonomic identifier

11578 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA negative-strand viruses › Bunyaviridae › Orthobunyavirus › California encephalitis virus › Bunyavirus La Crosse

Virus host

Aedes triseriatus (Eastern treehole mosquito) (Ochlerotatus triseriatus) [TaxID: 7162]
Cervidae (deer) [TaxID: 9850]
Homo sapiens (Human) [TaxID: 9606]
Tamias [TaxID: 13712]

Protein attributes

Sequence length	1441 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Glycoprotein G1 and glycoprotein G2 interact with each other and are present at the surface of the virion. They are able to attach the virion to a cell receptor and to promote fusion of membranes after endocytosis of the virion By similarity.
Subunit structure	G1 and G2 interacts with each other By similarity.
Subcellular location	Glycoprotein G1: Virion membrane; Single-pass type I membrane protein Potential. Host Golgi apparatus membrane; Single-pass type I membrane protein Potential. Host endoplasmic reticulum membrane; Single-pass type I membrane protein Potential. Note: G1 alone is retained in the membrane of the endoplasmic reticulum, but not transported to the Golgi. Coexpression of G1 and G2 results in efficient transport of G1 to the Golgi complex, indicating that their interaction is essential for proper targeting to this organelle, where virion budding occurs By similarity. Glycoprotein G2: Virion membrane; Single-pass type I membrane protein Potential. Host Golgi apparatus membrane; Single-pass type I membrane protein Potential. Note: G2 is retained in the Golgi complex through a Golgi retention signal, which resides in the G2 transmembrane region By similarity. Non-structural protein NSm: Host Golgi apparatus membrane; Multi-pass membrane protein Potential.
Post-translational modification	Specific enzymatic cleavages in vivo yield mature proteins including nonstructural protein NSm, glycoprotein G1, and glycoprotein G2.
Sequence similarities	Belongs to the orthobunyavirus envelope glycoprotein family.

Ontologies

Keywords
Biological process	Fusion of virus membrane with host endosomal membrane Fusion of virus membrane with host membrane Host-virus interaction Viral attachment to host cell Viral penetration into host cytoplasm Virus entry into host cell
Cellular component	Host Golgi apparatus Host endoplasmic reticulum Host membrane Membrane Virion
Domain	Signal Transmembrane Transmembrane helix
PTM	Glycoprotein
Gene Ontology (GO)
Biological_process	viral attachment to host cell Inferred from electronic annotation. Source: UniProtKB-KW viral entry into host cell Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	host cell Golgi membrane Inferred from electronic annotation. Source: UniProtKB-SubCell host cell endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW virion membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 13	13	Potential
	Chain	14 – 1441	1428	Envelope glycoprotein		PRO_0000036790
	Chain	22 – 299	278	Glycoprotein G2 By similarity		PRO_0000036791
	Chain	300 – 473	174	Non-structural protein NSm By similarity		PRO_0000036792
	Chain	474 – 1441	968	Glycoprotein G1 By similarity		PRO_0000036793
Regions
	Topological domain	14 – 200	187	Lumenal Potential
	Transmembrane	201 – 221	21	Helical; Potential
	Topological domain	222 – 305	84	Cytoplasmic Potential
	Transmembrane	306 – 326	21	Helical; Potential
	Topological domain	327 – 365	39	Lumenal Potential
	Transmembrane	366 – 386	21	Helical; Potential
	Topological domain	387 – 452	66	Cytoplasmic Potential
	Transmembrane	453 – 473	21	Helical; Potential
	Topological domain	474 – 1395	922	Lumenal Potential
	Transmembrane	1396 – 1416	21	Helical; Potential
	Topological domain	1417 – 1441	25	Cytoplasmic Potential
Sites
	Site	473 – 474	2	Cleavage; by host signal peptidase By similarity
Amino acid modifications
	Glycosylation	57	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	490	1	N-linked (GlcNAc...); by host Potential
	Glycosylation	1177	1	N-linked (GlcNAc...); by host Potential
Experimental info
	Sequence conflict	40	1	C → S Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P09612 [UniParc]. Last modified February 1, 1996. Version 3. Checksum: 50973CE30973C55B	FASTA	1,441	162,541
	10 20 30 40 50 60 MIRMLVLIVV TAASPVYQRC FQDGAIVKQN PSKEAVTEVC LKDDVSMIKT EARYVKNATG 70 80 90 100 110 120 VFSNNVAIRK WLVSDWHDCR PKKIVGGHIN VIEVGDDLSL HTESYVCSAD CTIGVDKETA 130 140 150 160 170 180 QVRLQTDTTN HFEIAGTTVK SGWFKSTTYI TLDQTCEHLK VSCAPKSVQF HACFNQHMSC 190 200 210 220 230 240 VRFLHRTILP GSIANSICQN IEIIILVTLT LLIFILLSIL SKTYICYLLM PIFIPIAYMY 250 260 270 280 290 300 GVIYNKSCKK CKLCGLVYHP FTECGTHCVC GARYDTSDRM KLHRASGLCP GYKSLRAARV 310 320 330 340 350 360 MCKSKGPASI LSIITAVLVL TFVTPINSMV LGESKETFEL EELPDDMLEM ALRINSYYFT 370 380 390 400 410 420 CILNYAVSWG LIIAGLLVGL IFKKYQHRFL NIYAMYCEEC NMYHDKSGLK RHGDFTNKCR 430 440 450 460 470 480 QCTCGQYEDA TGLITHRKTY NCLVQYKAKW MMNFLIIYIF LILIKDSAIV GQATGTDFTT 490 500 510 520 530 540 CLETESINWN CTGPFLNLGN CQKQQKKEPY TNIATQLKGL KAISVLDIPI ITSIPDDIAG 550 560 570 580 590 600 ALRYIEEKED FHVQLTTEYA MLSKYCDYYT QFSDNSGYSQ TTWRVYLRSH DFEACILYPN 610 620 630 640 650 660 QHFCKCVKNG EKCSSSNWDF ANGMKNYYSG KQAKFDKDLN LALTALHHAF RGTSSAYIAA 670 680 690 700 710 720 MLSKKSNDDL IAYTNKIKAK FPGNALLKAI IDYIAYMKGL PEMANFKYDE FWDELLYKPN 730 740 750 760 770 780 PAKASNLARG KESSYNFKLA ISSKSIKTCK NVKDVACLSP RSGAIYSSII ACGEPNGPSV 790 800 810 820 830 840 YRKPSGGVFQ SSTDRSIYCL LDSHCLEEFE AISQEELDAV KKSKCWEIEY PDVRPLQESD 850 860 870 880 890 900 GAKSCRMKDS GNCNVATNRW PVMQCENDKF YYSELQKDYD KTQDIGHYCL SPGCTTIRYP 910 920 930 940 950 960 INPKHISNCN WQVSRSSIAK IDVHNVEDIE QYKKAITQKL QTSLSLFKYA KTKNLPHIRP 970 980 990 1000 1010 1020 IYKYITMKET ETAEGIESAY IESEVPALAG TSVGFKINSK EGKHLLDVIA YVKSASYSSV 1030 1040 1050 1060 1070 1080 YAKLYSTGPT SGINTKHDEL CTGPCPANIN HQVGWLTFAR ERTSSWGCEE FGCLAVSDGC 1090 1100 1110 1120 1130 1140 VFGSCQDIIK EELSVYRKET EEVTNVELCL TFSDKTYCTN LNPVTPIITD LFEVQFKTVE 1150 1160 1170 1180 1190 1200 TYSLPRIVAV QNHEIKIGQI NDLGVYSKGC GNVQKVNGTV YGNGVPRFDY LCHLASRKEV 1210 1220 1230 1240 1250 1260 IVRKCFDNDY QACKFLQSPA SYRLEEDSGT VTIIDYKKIL GTIKMKAILG DVKYKTFADS 1270 1280 1290 1300 1310 1320 VDITAEGSCA GCINCFQNIH CELTLHTTIE ASCPIKSSCT VFHDRILVTP NEHKYALKIV 1330 1340 1350 1360 1370 1380 CTEKPGNTLT IKVCNTRIEA SMALVDAKPI IELAPVDQTA YIREKDERCK TWMCRVRDEG 1390 1400 1410 1420 1430 1440 LQVILEPFKN LFGSYIGIFY TFIISIIALL VIIYVLLPIC FKLRDTLRKH DDAYKREMKI R

References

[1]	"The sequence of the M RNA of an isolate of La Crosse virus." Grady L.J., Sanders M.L., Campbell W.P. J. Gen. Virol. 68:3057-3071(1987) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"Nucleotide sequence analyses and predicted coding of bunyavirus genome RNA species." Clerx-Van Haaster C.M., Akashi H., Auperin D.D., Bishop D.H.L. J. Virol. 41:119-128(1982) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 1-46.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D10370 Genomic RNA. Translation: BAA01201.1. J02231 Genomic RNA. No translation available.
PIR	GNVULC. A29377.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR005167. Bunya_G1. IPR005168. Bunya_G2. IPR026400. Bunya_nonstruc_pro_NSm. IPR014413. M_poly_OrthobunV. [Graphical view]
Pfam	PF03557. Bunya_G1. 1 hit. PF03563. Bunya_G2. 1 hit. [Graphical view]
PIRSF	PIRSF003944. M_poly_OrthobunV. 1 hit.
TIGRFAMs	TIGR04210. bunya_NSm. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GP_BUNL7

Accession

Primary (citable) accession number: P09612

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	February 1, 1996
Last modified:	April 3, 2013
This is version 78 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program