Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P09612 (GP_BUNL7) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 81. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Envelope glycoprotein

Short name=GP
Alternative name(s):
M polyprotein

Cleaved into the following 3 chains:

  1. Glycoprotein G2
    Short name=Gn
  2. Non-structural protein NSm
  3. Glycoprotein G1
    Short name=Gc
Gene names
Name:GP
OrganismBunyavirus La Crosse (isolate Aedes triseriatus/United States/L74/1974)
Taxonomic identifier11578 [NCBI]
Taxonomic lineageVirusesssRNA negative-strand virusesBunyaviridaeOrthobunyavirus
Virus hostCervidae (deer) [TaxID: 9850]
Homo sapiens (Human) [TaxID: 9606]
Ochlerotatus triseriatus (Eastern treehole mosquito) (Aedes triseriatus) [TaxID: 7162]
Tamias [TaxID: 13712]

Protein attributes

Sequence length1441 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Glycoprotein G1 and glycoprotein G2 interact with each other and are present at the surface of the virion. They are able to attach the virion to a cell receptor and to promote fusion of membranes after endocytosis of the virion By similarity.

Subunit structure

G1 and G2 interacts with each other By similarity.

Subcellular location

Glycoprotein G1: Virion membrane; Single-pass type I membrane protein Potential. Host Golgi apparatus membrane; Single-pass type I membrane protein Potential. Host endoplasmic reticulum membrane; Single-pass type I membrane protein Potential. Note: G1 alone is retained in the membrane of the endoplasmic reticulum, but not transported to the Golgi. Coexpression of G1 and G2 results in efficient transport of G1 to the Golgi complex, indicating that their interaction is essential for proper targeting to this organelle, where virion budding occurs By similarity.

Glycoprotein G2: Virion membrane; Single-pass type I membrane protein Potential. Host Golgi apparatus membrane; Single-pass type I membrane protein Potential. Note: G2 is retained in the Golgi complex through a Golgi retention signal, which resides in the G2 transmembrane region By similarity.

Non-structural protein NSm: Host Golgi apparatus membrane; Multi-pass membrane protein Potential.

Post-translational modification

Specific enzymatic cleavages in vivo yield mature proteins including nonstructural protein NSm, glycoprotein G1, and glycoprotein G2.

Sequence similarities

Belongs to the orthobunyavirus envelope glycoprotein family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1313 Potential
Chain14 – 14411428Envelope glycoprotein
PRO_0000036790
Chain22 – 299278Glycoprotein G2 By similarity
PRO_0000036791
Chain300 – 473174Non-structural protein NSm By similarity
PRO_0000036792
Chain474 – 1441968Glycoprotein G1 By similarity
PRO_0000036793

Regions

Topological domain14 – 200187Lumenal Potential
Transmembrane201 – 22121Helical; Potential
Topological domain222 – 30584Cytoplasmic Potential
Transmembrane306 – 32621Helical; Potential
Topological domain327 – 36539Lumenal Potential
Transmembrane366 – 38621Helical; Potential
Topological domain387 – 45266Cytoplasmic Potential
Transmembrane453 – 47321Helical; Potential
Topological domain474 – 1395922Lumenal Potential
Transmembrane1396 – 141621Helical; Potential
Topological domain1417 – 144125Cytoplasmic Potential

Sites

Site473 – 4742Cleavage; by host signal peptidase By similarity

Amino acid modifications

Glycosylation571N-linked (GlcNAc...); by host Potential
Glycosylation4901N-linked (GlcNAc...); by host Potential
Glycosylation11771N-linked (GlcNAc...); by host Potential

Experimental info

Sequence conflict401C → S Ref.2

Sequences

Sequence LengthMass (Da)Tools
P09612 [UniParc].

Last modified February 1, 1996. Version 3.
Checksum: 50973CE30973C55B

FASTA1,441162,541
        10         20         30         40         50         60 
MIRMLVLIVV TAASPVYQRC FQDGAIVKQN PSKEAVTEVC LKDDVSMIKT EARYVKNATG 

        70         80         90        100        110        120 
VFSNNVAIRK WLVSDWHDCR PKKIVGGHIN VIEVGDDLSL HTESYVCSAD CTIGVDKETA 

       130        140        150        160        170        180 
QVRLQTDTTN HFEIAGTTVK SGWFKSTTYI TLDQTCEHLK VSCAPKSVQF HACFNQHMSC 

       190        200        210        220        230        240 
VRFLHRTILP GSIANSICQN IEIIILVTLT LLIFILLSIL SKTYICYLLM PIFIPIAYMY 

       250        260        270        280        290        300 
GVIYNKSCKK CKLCGLVYHP FTECGTHCVC GARYDTSDRM KLHRASGLCP GYKSLRAARV 

       310        320        330        340        350        360 
MCKSKGPASI LSIITAVLVL TFVTPINSMV LGESKETFEL EELPDDMLEM ALRINSYYFT 

       370        380        390        400        410        420 
CILNYAVSWG LIIAGLLVGL IFKKYQHRFL NIYAMYCEEC NMYHDKSGLK RHGDFTNKCR 

       430        440        450        460        470        480 
QCTCGQYEDA TGLITHRKTY NCLVQYKAKW MMNFLIIYIF LILIKDSAIV GQATGTDFTT 

       490        500        510        520        530        540 
CLETESINWN CTGPFLNLGN CQKQQKKEPY TNIATQLKGL KAISVLDIPI ITSIPDDIAG 

       550        560        570        580        590        600 
ALRYIEEKED FHVQLTTEYA MLSKYCDYYT QFSDNSGYSQ TTWRVYLRSH DFEACILYPN 

       610        620        630        640        650        660 
QHFCKCVKNG EKCSSSNWDF ANGMKNYYSG KQAKFDKDLN LALTALHHAF RGTSSAYIAA 

       670        680        690        700        710        720 
MLSKKSNDDL IAYTNKIKAK FPGNALLKAI IDYIAYMKGL PEMANFKYDE FWDELLYKPN 

       730        740        750        760        770        780 
PAKASNLARG KESSYNFKLA ISSKSIKTCK NVKDVACLSP RSGAIYSSII ACGEPNGPSV 

       790        800        810        820        830        840 
YRKPSGGVFQ SSTDRSIYCL LDSHCLEEFE AISQEELDAV KKSKCWEIEY PDVRPLQESD 

       850        860        870        880        890        900 
GAKSCRMKDS GNCNVATNRW PVMQCENDKF YYSELQKDYD KTQDIGHYCL SPGCTTIRYP 

       910        920        930        940        950        960 
INPKHISNCN WQVSRSSIAK IDVHNVEDIE QYKKAITQKL QTSLSLFKYA KTKNLPHIRP 

       970        980        990       1000       1010       1020 
IYKYITMKET ETAEGIESAY IESEVPALAG TSVGFKINSK EGKHLLDVIA YVKSASYSSV 

      1030       1040       1050       1060       1070       1080 
YAKLYSTGPT SGINTKHDEL CTGPCPANIN HQVGWLTFAR ERTSSWGCEE FGCLAVSDGC 

      1090       1100       1110       1120       1130       1140 
VFGSCQDIIK EELSVYRKET EEVTNVELCL TFSDKTYCTN LNPVTPIITD LFEVQFKTVE 

      1150       1160       1170       1180       1190       1200 
TYSLPRIVAV QNHEIKIGQI NDLGVYSKGC GNVQKVNGTV YGNGVPRFDY LCHLASRKEV 

      1210       1220       1230       1240       1250       1260 
IVRKCFDNDY QACKFLQSPA SYRLEEDSGT VTIIDYKKIL GTIKMKAILG DVKYKTFADS 

      1270       1280       1290       1300       1310       1320 
VDITAEGSCA GCINCFQNIH CELTLHTTIE ASCPIKSSCT VFHDRILVTP NEHKYALKIV 

      1330       1340       1350       1360       1370       1380 
CTEKPGNTLT IKVCNTRIEA SMALVDAKPI IELAPVDQTA YIREKDERCK TWMCRVRDEG 

      1390       1400       1410       1420       1430       1440 
LQVILEPFKN LFGSYIGIFY TFIISIIALL VIIYVLLPIC FKLRDTLRKH DDAYKREMKI 


R 

« Hide

References

[1]"The sequence of the M RNA of an isolate of La Crosse virus."
Grady L.J., Sanders M.L., Campbell W.P.
J. Gen. Virol. 68:3057-3071(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]"Nucleotide sequence analyses and predicted coding of bunyavirus genome RNA species."
Clerx-Van Haaster C.M., Akashi H., Auperin D.D., Bishop D.H.L.
J. Virol. 41:119-128(1982) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 1-46.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D10370 Genomic RNA. Translation: BAA01201.1.
J02231 Genomic RNA. No translation available.
PIRGNVULC. A29377.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR005167. Bunya_G1.
IPR005168. Bunya_G2.
IPR026400. Bunya_nonstruc_pro_NSm.
IPR014413. M_poly_OrthobunV.
[Graphical view]
PfamPF03557. Bunya_G1. 1 hit.
PF03563. Bunya_G2. 1 hit.
[Graphical view]
PIRSFPIRSF003944. M_poly_OrthobunV. 1 hit.
TIGRFAMsTIGR04210. bunya_NSm. 1 hit.
ProtoNetSearch...

Entry information

Entry nameGP_BUNL7
AccessionPrimary (citable) accession number: P09612
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: February 1, 1996
Last modified: February 19, 2014
This is version 81 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families