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P09612

- GP_BUNL7

UniProt

P09612 - GP_BUNL7

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Protein

Envelope glycoprotein

Gene

GP

Organism
Bunyavirus La Crosse (isolate Aedes triseriatus/United States/L74/1974)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Glycoprotein G1 and glycoprotein G2 interact with each other and are present at the surface of the virion. They are able to attach the virion to a cell receptor and to promote fusion of membranes after endocytosis of the virion (By similarity).By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei473 – 4742Cleavage; by host signal peptidaseBy similarity

GO - Biological processi

  1. fusion of virus membrane with host endosome membrane Source: UniProtKB-KW
  2. modulation by virus of host morphology or physiology Source: InterPro
  3. virion attachment to host cell Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Fusion of virus membrane with host endosomal membrane, Fusion of virus membrane with host membrane, Host-virus interaction, Viral attachment to host cell, Viral penetration into host cytoplasm, Virus entry into host cell

Names & Taxonomyi

Protein namesi
Recommended name:
Envelope glycoprotein
Short name:
GP
Alternative name(s):
M polyprotein
Cleaved into the following 3 chains:
Gene namesi
Name:GP
OrganismiBunyavirus La Crosse (isolate Aedes triseriatus/United States/L74/1974)
Taxonomic identifieri11578 [NCBI]
Taxonomic lineageiVirusesssRNA negative-strand virusesBunyaviridaeOrthobunyavirus
Virus hostiCervidae (deer) [TaxID: 9850]
Homo sapiens (Human) [TaxID: 9606]
Ochlerotatus triseriatus (Eastern treehole mosquito) (Aedes triseriatus) [TaxID: 7162]
Tamias [TaxID: 13712]

Subcellular locationi

Chain Glycoprotein G1 : Virion membrane Curated; Single-pass type I membrane protein Curated. Host Golgi apparatus membrane Curated; Single-pass type I membrane protein Curated. Host endoplasmic reticulum membrane Curated; Single-pass type I membrane protein Curated
Note: G1 alone is retained in the membrane of the endoplasmic reticulum, but not transported to the Golgi. Coexpression of G1 and G2 results in efficient transport of G1 to the Golgi complex, indicating that their interaction is essential for proper targeting to this organelle, where virion budding occurs (By similarity).By similarity
Chain Glycoprotein G2 : Virion membrane Curated; Single-pass type I membrane protein Curated. Host Golgi apparatus membrane Curated; Single-pass type I membrane protein Curated
Note: G2 is retained in the Golgi complex through a Golgi retention signal, which resides in the G2 transmembrane region.By similarity

GO - Cellular componenti

  1. host cell endoplasmic reticulum Source: UniProtKB-KW
  2. host cell Golgi apparatus Source: UniProtKB-KW
  3. host cell membrane Source: UniProtKB-KW
  4. integral component of membrane Source: UniProtKB-KW
  5. virion Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host endoplasmic reticulum, Host Golgi apparatus, Host membrane, Membrane, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 1313Sequence AnalysisAdd
BLAST
Chaini14 – 14411428Envelope glycoproteinPRO_0000036790Add
BLAST
Chaini22 – 299278Glycoprotein G2By similarityPRO_0000036791Add
BLAST
Chaini300 – 473174Non-structural protein NSmBy similarityPRO_0000036792Add
BLAST
Chaini474 – 1441968Glycoprotein G1By similarityPRO_0000036793Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi57 – 571N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi490 – 4901N-linked (GlcNAc...); by hostSequence Analysis
Glycosylationi1177 – 11771N-linked (GlcNAc...); by hostSequence Analysis

Post-translational modificationi

Specific enzymatic cleavages in vivo yield mature proteins including nonstructural protein NSm, glycoprotein G1, and glycoprotein G2.

Keywords - PTMi

Glycoprotein

Interactioni

Subunit structurei

G1 and G2 interacts with each other.By similarity

Structurei

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini14 – 200187LumenalSequence AnalysisAdd
BLAST
Topological domaini222 – 30584CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini327 – 36539LumenalSequence AnalysisAdd
BLAST
Topological domaini387 – 45266CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini474 – 1395922LumenalSequence AnalysisAdd
BLAST
Topological domaini1417 – 144125CytoplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei201 – 22121HelicalSequence AnalysisAdd
BLAST
Transmembranei306 – 32621HelicalSequence AnalysisAdd
BLAST
Transmembranei366 – 38621HelicalSequence AnalysisAdd
BLAST
Transmembranei453 – 47321HelicalSequence AnalysisAdd
BLAST
Transmembranei1396 – 141621HelicalSequence AnalysisAdd
BLAST

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Family and domain databases

InterProiIPR005167. Bunya_G1.
IPR005168. Bunya_G2.
IPR026400. Bunya_nonstruc_pro_NSm.
IPR014413. M_poly_OrthobunV.
[Graphical view]
PfamiPF03557. Bunya_G1. 1 hit.
PF03563. Bunya_G2. 1 hit.
[Graphical view]
PIRSFiPIRSF003944. M_poly_OrthobunV. 1 hit.
TIGRFAMsiTIGR04210. bunya_NSm. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09612-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MIRMLVLIVV TAASPVYQRC FQDGAIVKQN PSKEAVTEVC LKDDVSMIKT
60 70 80 90 100
EARYVKNATG VFSNNVAIRK WLVSDWHDCR PKKIVGGHIN VIEVGDDLSL
110 120 130 140 150
HTESYVCSAD CTIGVDKETA QVRLQTDTTN HFEIAGTTVK SGWFKSTTYI
160 170 180 190 200
TLDQTCEHLK VSCAPKSVQF HACFNQHMSC VRFLHRTILP GSIANSICQN
210 220 230 240 250
IEIIILVTLT LLIFILLSIL SKTYICYLLM PIFIPIAYMY GVIYNKSCKK
260 270 280 290 300
CKLCGLVYHP FTECGTHCVC GARYDTSDRM KLHRASGLCP GYKSLRAARV
310 320 330 340 350
MCKSKGPASI LSIITAVLVL TFVTPINSMV LGESKETFEL EELPDDMLEM
360 370 380 390 400
ALRINSYYFT CILNYAVSWG LIIAGLLVGL IFKKYQHRFL NIYAMYCEEC
410 420 430 440 450
NMYHDKSGLK RHGDFTNKCR QCTCGQYEDA TGLITHRKTY NCLVQYKAKW
460 470 480 490 500
MMNFLIIYIF LILIKDSAIV GQATGTDFTT CLETESINWN CTGPFLNLGN
510 520 530 540 550
CQKQQKKEPY TNIATQLKGL KAISVLDIPI ITSIPDDIAG ALRYIEEKED
560 570 580 590 600
FHVQLTTEYA MLSKYCDYYT QFSDNSGYSQ TTWRVYLRSH DFEACILYPN
610 620 630 640 650
QHFCKCVKNG EKCSSSNWDF ANGMKNYYSG KQAKFDKDLN LALTALHHAF
660 670 680 690 700
RGTSSAYIAA MLSKKSNDDL IAYTNKIKAK FPGNALLKAI IDYIAYMKGL
710 720 730 740 750
PEMANFKYDE FWDELLYKPN PAKASNLARG KESSYNFKLA ISSKSIKTCK
760 770 780 790 800
NVKDVACLSP RSGAIYSSII ACGEPNGPSV YRKPSGGVFQ SSTDRSIYCL
810 820 830 840 850
LDSHCLEEFE AISQEELDAV KKSKCWEIEY PDVRPLQESD GAKSCRMKDS
860 870 880 890 900
GNCNVATNRW PVMQCENDKF YYSELQKDYD KTQDIGHYCL SPGCTTIRYP
910 920 930 940 950
INPKHISNCN WQVSRSSIAK IDVHNVEDIE QYKKAITQKL QTSLSLFKYA
960 970 980 990 1000
KTKNLPHIRP IYKYITMKET ETAEGIESAY IESEVPALAG TSVGFKINSK
1010 1020 1030 1040 1050
EGKHLLDVIA YVKSASYSSV YAKLYSTGPT SGINTKHDEL CTGPCPANIN
1060 1070 1080 1090 1100
HQVGWLTFAR ERTSSWGCEE FGCLAVSDGC VFGSCQDIIK EELSVYRKET
1110 1120 1130 1140 1150
EEVTNVELCL TFSDKTYCTN LNPVTPIITD LFEVQFKTVE TYSLPRIVAV
1160 1170 1180 1190 1200
QNHEIKIGQI NDLGVYSKGC GNVQKVNGTV YGNGVPRFDY LCHLASRKEV
1210 1220 1230 1240 1250
IVRKCFDNDY QACKFLQSPA SYRLEEDSGT VTIIDYKKIL GTIKMKAILG
1260 1270 1280 1290 1300
DVKYKTFADS VDITAEGSCA GCINCFQNIH CELTLHTTIE ASCPIKSSCT
1310 1320 1330 1340 1350
VFHDRILVTP NEHKYALKIV CTEKPGNTLT IKVCNTRIEA SMALVDAKPI
1360 1370 1380 1390 1400
IELAPVDQTA YIREKDERCK TWMCRVRDEG LQVILEPFKN LFGSYIGIFY
1410 1420 1430 1440
TFIISIIALL VIIYVLLPIC FKLRDTLRKH DDAYKREMKI R
Length:1,441
Mass (Da):162,541
Last modified:February 1, 1996 - v3
Checksum:i50973CE30973C55B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti40 – 401C → S(PubMed:7086954)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D10370 Genomic RNA. Translation: BAA01201.1.
J02231 Genomic RNA. No translation available.
PIRiA29377. GNVULC.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D10370 Genomic RNA. Translation: BAA01201.1 .
J02231 Genomic RNA. No translation available.
PIRi A29377. GNVULC.

3D structure databases

ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR005167. Bunya_G1.
IPR005168. Bunya_G2.
IPR026400. Bunya_nonstruc_pro_NSm.
IPR014413. M_poly_OrthobunV.
[Graphical view ]
Pfami PF03557. Bunya_G1. 1 hit.
PF03563. Bunya_G2. 1 hit.
[Graphical view ]
PIRSFi PIRSF003944. M_poly_OrthobunV. 1 hit.
TIGRFAMsi TIGR04210. bunya_NSm. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "The sequence of the M RNA of an isolate of La Crosse virus."
    Grady L.J., Sanders M.L., Campbell W.P.
    J. Gen. Virol. 68:3057-3071(1987) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
  2. "Nucleotide sequence analyses and predicted coding of bunyavirus genome RNA species."
    Clerx-Van Haaster C.M., Akashi H., Auperin D.D., Bishop D.H.L.
    J. Virol. 41:119-128(1982) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA] OF 1-46.

Entry informationi

Entry nameiGP_BUNL7
AccessioniPrimary (citable) accession number: P09612
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: February 1, 1996
Last modified: October 29, 2014
This is version 83 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3