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P09605 (KCRS_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 104. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Creatine kinase S-type, mitochondrial

EC=2.7.3.2
Alternative name(s):
Basic-type mitochondrial creatine kinase
Short name=Mib-CK
Sarcomeric mitochondrial creatine kinase
Short name=S-MtCK
Gene names
Name:Ckmt2
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length419 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

Catalytic activity

ATP + creatine = ADP + phosphocreatine.

Subunit structure

Exists as an octamer composed of four CKMT2 homodimers.

Subcellular location

Mitochondrion inner membrane; Peripheral membrane protein; Intermembrane side.

Tissue specificity

Sarcomere-specific. Found only in heart and skeletal muscles.

Miscellaneous

Mitochondrial creatine kinase binds cardiolipin.

Sequence similarities

Belongs to the ATP:guanido phosphotransferase family.

Contains 1 phosphagen kinase C-terminal domain.

Contains 1 phosphagen kinase N-terminal domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 3939Mitochondrion Ref.2
Chain40 – 419380Creatine kinase S-type, mitochondrial
PRO_0000016598

Regions

Domain46 – 13287Phosphagen kinase N-terminal
Domain159 – 401243Phosphagen kinase C-terminal
Nucleotide binding162 – 1665ATP By similarity
Nucleotide binding354 – 3596ATP By similarity
Region40 – 6425Cardiolipin-binding

Sites

Binding site2251ATP By similarity
Binding site2701ATP By similarity
Binding site3261ATP By similarity
Binding site3691ATP By similarity

Experimental info

Sequence conflict451H → C AA sequence Ref.2
Sequence conflict511S → H AA sequence Ref.2
Sequence conflict71 – 722TI → IK AA sequence Ref.2
Sequence conflict77 – 793RNK → NCG AA sequence Ref.2

Sequences

Sequence LengthMass (Da)Tools
P09605 [UniParc].

Last modified May 1, 1992. Version 2.
Checksum: 106041417F5D412F

FASTA41947,385
        10         20         30         40         50         60 
MASAFSKLLT GRNASLLFTT LGTSALTTGY LLNRQKVSAD AREQHKLFPP SADYPDLRKH 

        70         80         90        100        110        120 
NNCMAECLTP TIYAKLRNKM TPSGYTLDQC IQTGVDNPGH PFIKTVGMVA GDEESYEVFA 

       130        140        150        160        170        180 
DLFDPVIKLR HNGYDPRLMK HPADLDASKI THGQFDERYV LSSRVRTGRS IRGLSLPPAC 

       190        200        210        220        230        240 
SRAERREVEN VAITALGGLK GDLAGRYYKL SEMTEQDQQR LIDDHFLFDK PVSPLLTCAG 

       250        260        270        280        290        300 
MARDWPDARG IWHNYDKTFL IWINEEDHTR VISMEKGGNM KRVFERFCRG LKEVERLIQE 

       310        320        330        340        350        360 
RGWEFMWNER LGYILTCPSN LGTGLRAGVH VRIPKLSKDP RFSKILENLR LQKRGTGGVD 

       370        380        390        400        410 
TAAVADVYDI SNIDRIGRSE VELVQIVIDG VNYLVDCEKK LERGQDIKVP PPLPQFGRK 

« Hide

References

[1]"Structural characterization and tissue-specific expression of the mRNAs encoding isoenzymes from two rat mitochondrial creatine kinase genes."
Payne R.M., Haas R.C., Strauss A.W.
Biochim. Biophys. Acta 1089:352-361(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: Sprague-Dawley.
Tissue: Heart.
[2]"Identification and primary structure of the cardiolipin-binding domain of mitochondrial creatine kinase."
Cheneval D., Carafoli E.
Eur. J. Biochem. 171:1-9(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 40-79.
Tissue: Heart.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X59736 mRNA. Translation: CAA42414.1.
PIRS17188.
UniGeneRn.162549.

3D structure databases

ProteinModelPortalP09605.
SMRP09605. Positions 47-413.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP09605.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

UCSCRGD:61977. rat.

Organism-specific databases

RGD61977. Ckmt2.

Phylogenomic databases

HOVERGENHBG001339.
PhylomeDBP09605.

Gene expression databases

GenevestigatorP09605.

Family and domain databases

Gene3D1.10.135.10. 1 hit.
3.30.590.10. 1 hit.
InterProIPR022415. ATP-guanido_PTrfase_AS.
IPR022414. ATP-guanido_PTrfase_cat.
IPR022413. ATP-guanido_PTrfase_N.
IPR014746. Gln_synth/guanido_kin_cat_dom.
[Graphical view]
PfamPF00217. ATP-gua_Ptrans. 1 hit.
PF02807. ATP-gua_PtransN. 1 hit.
[Graphical view]
SUPFAMSSF48034. SSF48034. 1 hit.
PROSITEPS00112. PHOSPHAGEN_KINASE. 1 hit.
PS51510. PHOSPHAGEN_KINASE_C. 1 hit.
PS51509. PHOSPHAGEN_KINASE_N. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKCRS_RAT
AccessionPrimary (citable) accession number: P09605
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: May 1, 1992
Last modified: April 16, 2014
This is version 104 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families