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P09605

- KCRS_RAT

UniProt

P09605 - KCRS_RAT

Protein

Creatine kinase S-type, mitochondrial

Gene

Ckmt2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 105 (01 Oct 2014)
      Sequence version 2 (01 May 1992)
      Previous versions | rss
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    Functioni

    Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa.

    Catalytic activityi

    ATP + creatine = ADP + phosphocreatine.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei225 – 2251ATPPROSITE-ProRule annotation
    Binding sitei270 – 2701ATPPROSITE-ProRule annotation
    Binding sitei326 – 3261ATPPROSITE-ProRule annotation
    Binding sitei369 – 3691ATPPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi162 – 1665ATPPROSITE-ProRule annotation
    Nucleotide bindingi354 – 3596ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. creatine kinase activity Source: RGD

    GO - Biological processi

    1. phosphocreatine biosynthetic process Source: RGD

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Creatine kinase S-type, mitochondrial (EC:2.7.3.2)
    Alternative name(s):
    Basic-type mitochondrial creatine kinase
    Short name:
    Mib-CK
    Sarcomeric mitochondrial creatine kinase
    Short name:
    S-MtCK
    Gene namesi
    Name:Ckmt2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Unplaced

    Organism-specific databases

    RGDi61977. Ckmt2.

    Subcellular locationi

    GO - Cellular componenti

    1. mitochondrial inner membrane Source: UniProtKB-SubCell
    2. mitochondrion Source: RGD
    3. sarcomere Source: RGD

    Keywords - Cellular componenti

    Membrane, Mitochondrion, Mitochondrion inner membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 3939Mitochondrion1 PublicationAdd
    BLAST
    Chaini40 – 419380Creatine kinase S-type, mitochondrialPRO_0000016598Add
    BLAST

    Proteomic databases

    PRIDEiP09605.

    Expressioni

    Tissue specificityi

    Sarcomere-specific. Found only in heart and skeletal muscles.

    Gene expression databases

    GenevestigatoriP09605.

    Interactioni

    Subunit structurei

    Exists as an octamer composed of four CKMT2 homodimers.

    Structurei

    3D structure databases

    ProteinModelPortaliP09605.
    SMRiP09605. Positions 47-413.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini46 – 13287Phosphagen kinase N-terminalPROSITE-ProRule annotationAdd
    BLAST
    Domaini159 – 401243Phosphagen kinase C-terminalPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni40 – 6425Cardiolipin-bindingAdd
    BLAST

    Sequence similaritiesi

    Belongs to the ATP:guanido phosphotransferase family.PROSITE-ProRule annotation
    Contains 1 phosphagen kinase C-terminal domain.PROSITE-ProRule annotation
    Contains 1 phosphagen kinase N-terminal domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    HOVERGENiHBG001339.
    PhylomeDBiP09605.

    Family and domain databases

    Gene3Di1.10.135.10. 1 hit.
    3.30.590.10. 1 hit.
    InterProiIPR022415. ATP-guanido_PTrfase_AS.
    IPR022414. ATP-guanido_PTrfase_cat.
    IPR022413. ATP-guanido_PTrfase_N.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    [Graphical view]
    PfamiPF00217. ATP-gua_Ptrans. 1 hit.
    PF02807. ATP-gua_PtransN. 1 hit.
    [Graphical view]
    SUPFAMiSSF48034. SSF48034. 1 hit.
    PROSITEiPS00112. PHOSPHAGEN_KINASE. 1 hit.
    PS51510. PHOSPHAGEN_KINASE_C. 1 hit.
    PS51509. PHOSPHAGEN_KINASE_N. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P09605-1 [UniParc]FASTAAdd to Basket

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    MASAFSKLLT GRNASLLFTT LGTSALTTGY LLNRQKVSAD AREQHKLFPP    50
    SADYPDLRKH NNCMAECLTP TIYAKLRNKM TPSGYTLDQC IQTGVDNPGH 100
    PFIKTVGMVA GDEESYEVFA DLFDPVIKLR HNGYDPRLMK HPADLDASKI 150
    THGQFDERYV LSSRVRTGRS IRGLSLPPAC SRAERREVEN VAITALGGLK 200
    GDLAGRYYKL SEMTEQDQQR LIDDHFLFDK PVSPLLTCAG MARDWPDARG 250
    IWHNYDKTFL IWINEEDHTR VISMEKGGNM KRVFERFCRG LKEVERLIQE 300
    RGWEFMWNER LGYILTCPSN LGTGLRAGVH VRIPKLSKDP RFSKILENLR 350
    LQKRGTGGVD TAAVADVYDI SNIDRIGRSE VELVQIVIDG VNYLVDCEKK 400
    LERGQDIKVP PPLPQFGRK 419
    Length:419
    Mass (Da):47,385
    Last modified:May 1, 1992 - v2
    Checksum:i106041417F5D412F
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti45 – 451H → C AA sequence (PubMed:3338460)Curated
    Sequence conflicti51 – 511S → H AA sequence (PubMed:3338460)Curated
    Sequence conflicti71 – 722TI → IK AA sequence (PubMed:3338460)Curated
    Sequence conflicti77 – 793RNK → NCG AA sequence (PubMed:3338460)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59736 mRNA. Translation: CAA42414.1.
    PIRiS17188.
    UniGeneiRn.162549.

    Genome annotation databases

    UCSCiRGD:61977. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X59736 mRNA. Translation: CAA42414.1 .
    PIRi S17188.
    UniGenei Rn.162549.

    3D structure databases

    ProteinModelPortali P09605.
    SMRi P09605. Positions 47-413.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P09605.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    UCSCi RGD:61977. rat.

    Organism-specific databases

    RGDi 61977. Ckmt2.

    Phylogenomic databases

    HOVERGENi HBG001339.
    PhylomeDBi P09605.

    Gene expression databases

    Genevestigatori P09605.

    Family and domain databases

    Gene3Di 1.10.135.10. 1 hit.
    3.30.590.10. 1 hit.
    InterProi IPR022415. ATP-guanido_PTrfase_AS.
    IPR022414. ATP-guanido_PTrfase_cat.
    IPR022413. ATP-guanido_PTrfase_N.
    IPR014746. Gln_synth/guanido_kin_cat_dom.
    [Graphical view ]
    Pfami PF00217. ATP-gua_Ptrans. 1 hit.
    PF02807. ATP-gua_PtransN. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48034. SSF48034. 1 hit.
    PROSITEi PS00112. PHOSPHAGEN_KINASE. 1 hit.
    PS51510. PHOSPHAGEN_KINASE_C. 1 hit.
    PS51509. PHOSPHAGEN_KINASE_N. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structural characterization and tissue-specific expression of the mRNAs encoding isoenzymes from two rat mitochondrial creatine kinase genes."
      Payne R.M., Haas R.C., Strauss A.W.
      Biochim. Biophys. Acta 1089:352-361(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: Sprague-Dawley.
      Tissue: Heart.
    2. "Identification and primary structure of the cardiolipin-binding domain of mitochondrial creatine kinase."
      Cheneval D., Carafoli E.
      Eur. J. Biochem. 171:1-9(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 40-79.
      Tissue: Heart.

    Entry informationi

    Entry nameiKCRS_RAT
    AccessioniPrimary (citable) accession number: P09605
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: May 1, 1992
    Last modified: October 1, 2014
    This is version 105 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Mitochondrial creatine kinase binds cardiolipin.

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3