P09599 (PHL1_BACCE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Sphingomyelinase C
Short name=SMase
EC=3.1.4.12

Alternative name(s):
Cereolysin B
SMPLC
Sphingomyelin phosphodiesterase

Gene names

Name:

sph

Organism

Bacillus cereus

Taxonomic identifier

1396 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus › Bacillus cereus group

Protein attributes

Sequence length	333 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Required, with sphingomyelinase, to effect target cell lysis (hemolysis).
Catalytic activity	Sphingomyelin + H2O = N-acylsphingosine + choline phosphate.
Cofactor	Magnesium.
Enzyme regulation	Activated by cobalt and manganese ions.
Subcellular location	Secreted.
Sequence similarities	Belongs to the neutral sphingomyelinase family.
Sequence caution	The sequence CAA45501.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Cytolysis Hemolysis
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase
PTM	Disulfide bond
Gene Ontology (GO)
Biological process	cytolysis Inferred from electronic annotation. Source: UniProtKB-KW hemolysis in other organism Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	sphingomyelin phosphodiesterase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 27	27
	Chain	28 – 333	306	Sphingomyelinase C		PRO_0000019899
Amino acid modifications
	Disulfide bond	150 ↔ 186		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P09599 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 035EF97D690C8378	FASTA	333	36,949
	10 20 30 40 50 60 MKGKLLKGVL SFGIGLGVLY GGSSVQADTS TDQNNTLKVM THNVYMLSTN LYPNWGQSQR 70 80 90 100 110 120 ADLIGAADYI KNQDVVILNE VFDNSASDRL LGNLKKEYPN QTAVLGRSNG NEWDKTLGSY 130 140 150 160 170 180 SSSTPEDGGV AIVSKWPIVE KIQYVFAKGC GPDNLSNKGF VYTKIKKNDR FVHVIGTHLQ 190 200 210 220 230 240 AEDSMCGKTS PASVRTNQLK EIQDFIKNKN IPNDEYVLFG GDMNVNKINA ENNSDSEYAS 250 260 270 280 290 300 MFKTLHASIP SYTGHTATWD ATTNSIAKYN FPDSPAEYLD YIIASKDHAN PSFIENKVLQ 310 320 330 PKSPQWTVTS WLKKYTYDDY SDHYPVAATI SMK

References

[1]	"Bacillus cereus strain SE-1: nucleotide sequence of the sphingomyelinase C gene." Johansen T., Haugli F.B., Ikezawa H., Little C. Nucleic Acids Res. 16:10370-10370(1988) [PubMed: 2848222] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: SE-1.
[2]	"Nucleotide sequence of phospholipase C and sphingomyelinase genes from Bacillus cereus BKM-B164." Kuzmin N.P., Gavrilenko I.V., Krukov V.M., Karpov A.V. Bioorg. Khim. 19:133-138(1993) [PubMed: 8387306] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: VKM B-164.
[3]	"Cloning and sequencing of the gene encoding the phosphatidylcholine-preferring phospholipase C of Bacillus cereus." Johansen T., Holm T., Guddal P.H., Sletten K., Haugli F.B., Little C. Gene 65:293-304(1988) [PubMed: 3137122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-188. Strain: SE-1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X64141 Genomic DNA. Translation: CAA45503.1. X12854 Genomic DNA. Translation: CAA31333.1. X64140 Genomic DNA. Translation: CAA45501.1. Different initiation.
PIR	S01950.
3D structure databases
ProteinModelPortal	P09599.
SMR	P09599. Positions 34-332.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BRENDA	3.1.4.12. 648.
Family and domain databases
InterPro	IPR005135. Endo/exonuclease/phosphatase. IPR017766. Sphingomyelinase/PLipase_C. [Graphical view]
Pfam	PF03372. Exo_endo_phos. 1 hit. [Graphical view]
SUPFAM	SSF56219. Exo_endo_phos. 1 hit.
TIGRFAMs	TIGR03395. Sphingomy. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PHL1_BACCE

Accession

Primary (citable) accession number: P09599

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	October 19, 2011
This is version 74 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program