Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phospholipase C

Gene

plc

Organism
Bacillus cereus
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required, with sphingomyelinase, to effect target cell lysis (hemolysis).

Catalytic activityi

A phosphatidylcholine + H2O = 1,2-diacyl-sn-glycerol + phosphocholine.

Cofactori

Zn2+Note: Binds 3 Zn2+ ions per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi39Zinc 31
Metal bindingi52Zinc 31
Metal bindingi93Zinc 11
Metal bindingi107Zinc 11
Metal bindingi156Zinc 11
Metal bindingi160Zinc 11
Metal bindingi160Zinc 31
Metal bindingi166Zinc 21
Metal bindingi180Zinc 21
Metal bindingi184Zinc 21

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Cytolysis, Hemolysis

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.1.4.3. 648.
SABIO-RKP09598.

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase C (EC:3.1.4.3)
Short name:
PLC
Alternative name(s):
Cereolysin A
Phosphatidylcholine cholinephosphohydrolase
Gene namesi
Name:plc
OrganismiBacillus cereus
Taxonomic identifieri1396 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1293202.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
PropeptideiPRO_000002392725 – 382 PublicationsAdd BLAST14
ChainiPRO_000002392839 – 283Phospholipase CAdd BLAST245

Keywords - PTMi

Zymogen

Interactioni

Subunit structurei

Monomer.

Protein-protein interaction databases

STRINGi226900.BC0670.

Chemistry databases

BindingDBiP09598.

Structurei

Secondary structure

1283
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi43 – 45Combined sources3
Helixi47 – 49Combined sources3
Helixi51 – 65Combined sources15
Helixi72 – 80Combined sources9
Helixi82 – 91Combined sources10
Turni92 – 94Combined sources3
Turni96 – 103Combined sources8
Helixi105 – 107Combined sources3
Turni111 – 113Combined sources3
Helixi124 – 140Combined sources17
Helixi144 – 161Combined sources18
Turni164 – 169Combined sources6
Beta strandi174 – 176Combined sources3
Helixi179 – 190Combined sources12
Helixi191 – 194Combined sources4
Helixi210 – 223Combined sources14
Helixi225 – 227Combined sources3
Helixi231 – 240Combined sources10
Helixi244 – 280Combined sources37

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AH7X-ray1.50A39-283[»]
1P5XX-ray2.00A39-283[»]
1P6DX-ray2.00A39-283[»]
1P6EX-ray2.30A39-283[»]
2FFZX-ray2.05A39-283[»]
2FGNX-ray2.04A39-283[»]
2HUCX-ray1.90A39-283[»]
ProteinModelPortaliP09598.
SMRiP09598.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09598.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini39 – 283Zn-dependent PLCPROSITE-ProRule annotationAdd BLAST245

Sequence similaritiesi

Belongs to the bacterial zinc-metallophospholipase C family.PROSITE-ProRule annotation
Contains 1 Zn-dependent PLC domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

CDDicd11009. Zn_dep_PLPC. 1 hit.
Gene3Di1.10.575.10. 1 hit.
InterProiIPR008947. PLipase_C/P1_nuclease.
IPR029002. PLPC/GPLD1.
IPR001531. Zn_PLipaseC.
[Graphical view]
PfamiPF00882. Zn_dep_PLPC. 1 hit.
[Graphical view]
PRINTSiPR00479. PRPHPHLPASEC.
ProDomiPD003946. PLipaseC_Zn-bd_prok. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00770. Zn_dep_PLPC. 1 hit.
[Graphical view]
SUPFAMiSSF48537. SSF48537. 1 hit.
PROSITEiPS00384. PROKAR_ZN_DEPEND_PLPC_1. 1 hit.
PS51346. PROKAR_ZN_DEPEND_PLPC_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09598-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKKVLALAA AITVVAPLQS VAFAHENDGG SKIKIVHRWS AEDKHKEGVN
60 70 80 90 100
SHLWIVNRAI DIMSRNTTLV KQDRVAQLNE WRTELENGIY AADYENPYYD
110 120 130 140 150
NSTFASHFYD PDNGKTYIPF AKQAKETGAK YFKLAGESYK NKDMKQAFFY
160 170 180 190 200
LGLSLHYLGD VNQPMHAANF TNLSYPQGFH SKYENFVDTI KDNYKVTDGN
210 220 230 240 250
GYWNWKGTNP EEWIHGAAVV AKQDYSGIVN DNTKDWFVKA AVSQEYADKW
260 270 280
RAEVTPMTGK RLMDAQRVTA GYIQLWFDTY GDR
Length:283
Mass (Da):32,383
Last modified:July 1, 1989 - v1
Checksum:iAC5452EFF2E22B19
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti212E → D in strain: IAM 1208. 1
Natural varianti226S → A in strain: IAM 1208. 1
Natural varianti239K → R in strain: IAM 1208. 1
Natural varianti282D → N in strain: IAM 1208. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64141 Genomic DNA. Translation: CAA45502.1.
X12854 Genomic DNA. Translation: CAA31332.1.
X12711 Genomic DNA. Translation: CAA31213.1.
X64140 Genomic DNA. Translation: CAA45501.1. Different termination.
PIRiS18978. PS0197.
RefSeqiWP_000731014.1. NZ_LRRN01000422.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64141 Genomic DNA. Translation: CAA45502.1.
X12854 Genomic DNA. Translation: CAA31332.1.
X12711 Genomic DNA. Translation: CAA31213.1.
X64140 Genomic DNA. Translation: CAA45501.1. Different termination.
PIRiS18978. PS0197.
RefSeqiWP_000731014.1. NZ_LRRN01000422.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AH7X-ray1.50A39-283[»]
1P5XX-ray2.00A39-283[»]
1P6DX-ray2.00A39-283[»]
1P6EX-ray2.30A39-283[»]
2FFZX-ray2.05A39-283[»]
2FGNX-ray2.04A39-283[»]
2HUCX-ray1.90A39-283[»]
ProteinModelPortaliP09598.
SMRiP09598.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi226900.BC0670.

Chemistry databases

BindingDBiP09598.
ChEMBLiCHEMBL1293202.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

BRENDAi3.1.4.3. 648.
SABIO-RKP09598.

Miscellaneous databases

EvolutionaryTraceiP09598.

Family and domain databases

CDDicd11009. Zn_dep_PLPC. 1 hit.
Gene3Di1.10.575.10. 1 hit.
InterProiIPR008947. PLipase_C/P1_nuclease.
IPR029002. PLPC/GPLD1.
IPR001531. Zn_PLipaseC.
[Graphical view]
PfamiPF00882. Zn_dep_PLPC. 1 hit.
[Graphical view]
PRINTSiPR00479. PRPHPHLPASEC.
ProDomiPD003946. PLipaseC_Zn-bd_prok. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00770. Zn_dep_PLPC. 1 hit.
[Graphical view]
SUPFAMiSSF48537. SSF48537. 1 hit.
PROSITEiPS00384. PROKAR_ZN_DEPEND_PLPC_1. 1 hit.
PS51346. PROKAR_ZN_DEPEND_PLPC_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPHLC_BACCE
AccessioniPrimary (citable) accession number: P09598
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: November 2, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.