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P09598

- PHLC_BACCE

UniProt

P09598 - PHLC_BACCE

Protein

Phospholipase C

Gene

plc

Organism
Bacillus cereus
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 103 (01 Oct 2014)
      Sequence version 1 (01 Jul 1989)
      Previous versions | rss
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    Functioni

    Required, with sphingomyelinase, to effect target cell lysis (hemolysis).

    Catalytic activityi

    A phosphatidylcholine + H2O = 1,2-diacyl-sn-glycerol + phosphocholine.

    Cofactori

    Binds 3 zinc ions per subunit.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi39 – 391Zinc 3
    Metal bindingi52 – 521Zinc 3
    Metal bindingi93 – 931Zinc 1
    Metal bindingi107 – 1071Zinc 1
    Metal bindingi156 – 1561Zinc 1
    Metal bindingi160 – 1601Zinc 1
    Metal bindingi160 – 1601Zinc 3
    Metal bindingi166 – 1661Zinc 2
    Metal bindingi180 – 1801Zinc 2
    Metal bindingi184 – 1841Zinc 2

    GO - Molecular functioni

    1. phosphatidylcholine phospholipase C activity Source: UniProtKB-EC
    2. zinc ion binding Source: InterPro

    GO - Biological processi

    1. hemolysis in other organism Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Cytolysis, Hemolysis

    Keywords - Ligandi

    Metal-binding, Zinc

    Enzyme and pathway databases

    SABIO-RKP09598.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phospholipase C (EC:3.1.4.3)
    Short name:
    PLC
    Alternative name(s):
    Cereolysin A
    Phosphatidylcholine cholinephosphohydrolase
    Gene namesi
    Name:plc
    OrganismiBacillus cereus
    Taxonomic identifieri1396 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Sequence AnalysisAdd
    BLAST
    Propeptidei25 – 38142 PublicationsPRO_0000023927Add
    BLAST
    Chaini39 – 283245Phospholipase CPRO_0000023928Add
    BLAST

    Keywords - PTMi

    Zymogen

    Interactioni

    Subunit structurei

    Monomer.

    Structurei

    Secondary structure

    1
    283
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi43 – 453
    Helixi47 – 493
    Helixi51 – 6515
    Helixi72 – 809
    Helixi82 – 9110
    Turni92 – 943
    Turni96 – 1038
    Helixi105 – 1073
    Turni111 – 1133
    Helixi124 – 14017
    Helixi144 – 16118
    Turni164 – 1696
    Beta strandi174 – 1763
    Helixi179 – 19012
    Helixi191 – 1944
    Helixi210 – 22314
    Helixi225 – 2273
    Helixi231 – 24010
    Helixi244 – 28037

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1AH7X-ray1.50A39-283[»]
    1P5XX-ray2.00A39-283[»]
    1P6DX-ray2.00A39-283[»]
    1P6EX-ray2.30A39-283[»]
    2FFZX-ray2.05A39-283[»]
    2FGNX-ray2.04A39-283[»]
    2HUCX-ray1.90A39-283[»]
    ProteinModelPortaliP09598.
    SMRiP09598. Positions 39-283.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP09598.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini39 – 283245Zn-dependent PLCPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Belongs to the bacterial zinc-metallophospholipase C family.PROSITE-ProRule annotation
    Contains 1 Zn-dependent PLC domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di1.10.575.10. 1 hit.
    InterProiIPR008947. PLipase_C/P1_nuclease.
    IPR001531. PLipaseC_domain.
    IPR029002. PLPC/GPLD1.
    [Graphical view]
    PfamiPF00882. Zn_dep_PLPC. 1 hit.
    [Graphical view]
    PRINTSiPR00479. PRPHPHLPASEC.
    ProDomiPD003946. PLipaseC_Zn-bd_prok. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SMARTiSM00770. Zn_dep_PLPC. 1 hit.
    [Graphical view]
    SUPFAMiSSF48537. SSF48537. 1 hit.
    PROSITEiPS00384. PROKAR_ZN_DEPEND_PLPC_1. 1 hit.
    PS51346. PROKAR_ZN_DEPEND_PLPC_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P09598-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKKKVLALAA AITVVAPLQS VAFAHENDGG SKIKIVHRWS AEDKHKEGVN    50
    SHLWIVNRAI DIMSRNTTLV KQDRVAQLNE WRTELENGIY AADYENPYYD 100
    NSTFASHFYD PDNGKTYIPF AKQAKETGAK YFKLAGESYK NKDMKQAFFY 150
    LGLSLHYLGD VNQPMHAANF TNLSYPQGFH SKYENFVDTI KDNYKVTDGN 200
    GYWNWKGTNP EEWIHGAAVV AKQDYSGIVN DNTKDWFVKA AVSQEYADKW 250
    RAEVTPMTGK RLMDAQRVTA GYIQLWFDTY GDR 283
    Length:283
    Mass (Da):32,383
    Last modified:July 1, 1989 - v1
    Checksum:iAC5452EFF2E22B19
    GO

    Natural variant

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Natural varianti212 – 2121E → D in strain: IAM 1208.
    Natural varianti226 – 2261S → A in strain: IAM 1208.
    Natural varianti239 – 2391K → R in strain: IAM 1208.
    Natural varianti282 – 2821D → N in strain: IAM 1208.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X64141 Genomic DNA. Translation: CAA45502.1.
    X12854 Genomic DNA. Translation: CAA31332.1.
    X12711 Genomic DNA. Translation: CAA31213.1.
    X64140 Genomic DNA. Translation: CAA45501.1. Different termination.
    PIRiS18978. PS0197.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X64141 Genomic DNA. Translation: CAA45502.1 .
    X12854 Genomic DNA. Translation: CAA31332.1 .
    X12711 Genomic DNA. Translation: CAA31213.1 .
    X64140 Genomic DNA. Translation: CAA45501.1 . Different termination.
    PIRi S18978. PS0197.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1AH7 X-ray 1.50 A 39-283 [» ]
    1P5X X-ray 2.00 A 39-283 [» ]
    1P6D X-ray 2.00 A 39-283 [» ]
    1P6E X-ray 2.30 A 39-283 [» ]
    2FFZ X-ray 2.05 A 39-283 [» ]
    2FGN X-ray 2.04 A 39-283 [» ]
    2HUC X-ray 1.90 A 39-283 [» ]
    ProteinModelPortali P09598.
    SMRi P09598. Positions 39-283.
    ModBasei Search...
    MobiDBi Search...

    Chemistry

    BindingDBi P09598.
    ChEMBLi CHEMBL1293202.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    SABIO-RK P09598.

    Miscellaneous databases

    EvolutionaryTracei P09598.

    Family and domain databases

    Gene3Di 1.10.575.10. 1 hit.
    InterProi IPR008947. PLipase_C/P1_nuclease.
    IPR001531. PLipaseC_domain.
    IPR029002. PLPC/GPLD1.
    [Graphical view ]
    Pfami PF00882. Zn_dep_PLPC. 1 hit.
    [Graphical view ]
    PRINTSi PR00479. PRPHPHLPASEC.
    ProDomi PD003946. PLipaseC_Zn-bd_prok. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SMARTi SM00770. Zn_dep_PLPC. 1 hit.
    [Graphical view ]
    SUPFAMi SSF48537. SSF48537. 1 hit.
    PROSITEi PS00384. PROKAR_ZN_DEPEND_PLPC_1. 1 hit.
    PS51346. PROKAR_ZN_DEPEND_PLPC_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequencing of the gene encoding the phosphatidylcholine-preferring phospholipase C of Bacillus cereus."
      Johansen T., Holm T., Guddal P.H., Sletten K., Haugli F.B., Little C.
      Gene 65:293-304(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: SE-1.
    2. "Nucleotide sequence of phospholipase C and sphingomyelinase genes from Bacillus cereus BKM-B164."
      Kuzmin N.P., Gavrilenko I.V., Krukov V.M., Karpov A.V.
      Bioorg. Khim. 19:133-138(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: VKM B-164.
    3. "Nucleotide sequence and expression in Escherichia coli of the gene coding for sphingomyelinase of Bacillus cereus."
      Yamada A., Tsukagoshi N., Udaka S., Sasaki T., Makino S., Nakamura S., Little C., Tomita M., Ikezawa H.
      Eur. J. Biochem. 175:213-220(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 166-283.
      Strain: IAM 1208.
    4. Cited for: PROTEIN SEQUENCE OF 39-65.
    5. "Kinetics of the hydrolysis of monodispersed and micellar phosphatidylcholines catalyzed by a phospholipase C from Bacillus cereus."
      Ikeda K., Inoue S., Amasaki C., Teshima K., Ikezawa H.
      J. Biochem. 110:88-95(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 39-49.
      Strain: IAM 1208.
    6. "High-resolution (1.5 A) crystal structure of phospholipase C from Bacillus cereus."
      Hough E., Hansen L.K., Birknes B., Jynge K., Hansen S., Hordvik A., Little C., Dodson E., Derewenda Z.
      Nature 338:357-360(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (1.5 ANGSTROMS).

    Entry informationi

    Entry nameiPHLC_BACCE
    AccessioniPrimary (citable) accession number: P09598
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 103 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3