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Protein

Phospholipase C

Gene

plc

Organism
Bacillus cereus
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required, with sphingomyelinase, to effect target cell lysis (hemolysis).

Catalytic activityi

A phosphatidylcholine + H2O = 1,2-diacyl-sn-glycerol + phosphocholine.

Cofactori

Zn2+Note: Binds 3 Zn2+ ions per subunit.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi39Zinc 31
Metal bindingi52Zinc 31
Metal bindingi93Zinc 11
Metal bindingi107Zinc 11
Metal bindingi156Zinc 11
Metal bindingi160Zinc 11
Metal bindingi160Zinc 31
Metal bindingi166Zinc 21
Metal bindingi180Zinc 21
Metal bindingi184Zinc 21

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processCytolysis, Hemolysis
LigandMetal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.1.4.3 648
SABIO-RKiP09598

Names & Taxonomyi

Protein namesi
Recommended name:
Phospholipase C (EC:3.1.4.3)
Short name:
PLC
Alternative name(s):
Cereolysin A
Phosphatidylcholine cholinephosphohydrolase
Gene namesi
Name:plc
OrganismiBacillus cereus
Taxonomic identifieri1396 [NCBI]
Taxonomic lineageiBacteriaFirmicutesBacilliBacillalesBacillaceaeBacillusBacillus cereus group

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1293202

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
PropeptideiPRO_000002392725 – 382 PublicationsAdd BLAST14
ChainiPRO_000002392839 – 283Phospholipase CAdd BLAST245

Keywords - PTMi

Zymogen

Interactioni

Subunit structurei

Monomer.

Chemistry databases

BindingDBiP09598

Structurei

Secondary structure

1283
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi43 – 45Combined sources3
Helixi47 – 49Combined sources3
Helixi51 – 65Combined sources15
Helixi72 – 80Combined sources9
Helixi82 – 91Combined sources10
Turni92 – 94Combined sources3
Turni96 – 103Combined sources8
Helixi105 – 107Combined sources3
Turni111 – 113Combined sources3
Helixi124 – 140Combined sources17
Helixi144 – 161Combined sources18
Turni164 – 169Combined sources6
Beta strandi174 – 176Combined sources3
Helixi179 – 190Combined sources12
Helixi191 – 194Combined sources4
Helixi210 – 223Combined sources14
Helixi225 – 227Combined sources3
Helixi231 – 240Combined sources10
Helixi244 – 280Combined sources37

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1AH7X-ray1.50A39-283[»]
1P5XX-ray2.00A39-283[»]
1P6DX-ray2.00A39-283[»]
1P6EX-ray2.30A39-283[»]
2FFZX-ray2.05A39-283[»]
2FGNX-ray2.04A39-283[»]
2HUCX-ray1.90A39-283[»]
ProteinModelPortaliP09598
SMRiP09598
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09598

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini39 – 283Zn-dependent PLCPROSITE-ProRule annotationAdd BLAST245

Sequence similaritiesi

Belongs to the bacterial zinc-metallophospholipase C family.PROSITE-ProRule annotation

Keywords - Domaini

Signal

Family and domain databases

CDDicd11009 Zn_dep_PLPC, 1 hit
Gene3Di1.10.575.10, 1 hit
InterProiView protein in InterPro
IPR008947 PLipase_C/P1_nuclease_dom_sf
IPR029002 PLPC/GPLD1
IPR001531 Zn_PLipaseC
PfamiView protein in Pfam
PF00882 Zn_dep_PLPC, 1 hit
PRINTSiPR00479 PRPHPHLPASEC
ProDomiView protein in ProDom or Entries sharing at least one domain
PD003946 PLipaseC_Zn-bd_prok, 1 hit
SMARTiView protein in SMART
SM00770 Zn_dep_PLPC, 1 hit
SUPFAMiSSF48537 SSF48537, 1 hit
PROSITEiView protein in PROSITE
PS00384 PROKAR_ZN_DEPEND_PLPC_1, 1 hit
PS51346 PROKAR_ZN_DEPEND_PLPC_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09598-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKKVLALAA AITVVAPLQS VAFAHENDGG SKIKIVHRWS AEDKHKEGVN
60 70 80 90 100
SHLWIVNRAI DIMSRNTTLV KQDRVAQLNE WRTELENGIY AADYENPYYD
110 120 130 140 150
NSTFASHFYD PDNGKTYIPF AKQAKETGAK YFKLAGESYK NKDMKQAFFY
160 170 180 190 200
LGLSLHYLGD VNQPMHAANF TNLSYPQGFH SKYENFVDTI KDNYKVTDGN
210 220 230 240 250
GYWNWKGTNP EEWIHGAAVV AKQDYSGIVN DNTKDWFVKA AVSQEYADKW
260 270 280
RAEVTPMTGK RLMDAQRVTA GYIQLWFDTY GDR
Length:283
Mass (Da):32,383
Last modified:July 1, 1989 - v1
Checksum:iAC5452EFF2E22B19
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural varianti212E → D in strain: IAM 1208. 1
Natural varianti226S → A in strain: IAM 1208. 1
Natural varianti239K → R in strain: IAM 1208. 1
Natural varianti282D → N in strain: IAM 1208. 1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64141 Genomic DNA Translation: CAA45502.1
X12854 Genomic DNA Translation: CAA31332.1
X12711 Genomic DNA Translation: CAA31213.1
X64140 Genomic DNA Translation: CAA45501.1 Different termination.
PIRiS18978 PS0197
RefSeqiWP_000731014.1, NZ_PPFT01000010.1

Similar proteinsi

Entry informationi

Entry nameiPHLC_BACCE
AccessioniPrimary (citable) accession number: P09598
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: May 23, 2018
This is version 125 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

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