Reviewed, UniProtKB/Swiss-Prot P09582 (ESTA_CANFA)

Names and origin

Protein names	Recommended name:     Arginine esterase     EC=3.4.21.35
Organism	Canis familiaris (Dog)
Taxonomic identifier	9615 [NCBI]
Taxonomic lineage	Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Carnivora › Caniformia › Canidae › Canis

Protein attributes

Sequence length	260 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	This serine protease is found in dog seminal plasma, its exact physiological function is not known.
Catalytic activity	Preferential cleavage of Arg-|-Xaa bonds in small molecule substrates. Highly selective action to release kallidin (lysyl-bradykinin) from kininogen involves hydrolysis of Met-|-Xaa or Leu-|-Xaa.
Induction	By androgens.
Sequence similarities	Belongs to the peptidase S1 family. Kallikrein subfamily. Contains 1 peptidase S1 domain.

Ontologies

Keywords
Domain	Signal
Molecular function	Hydrolase Protease Serine protease
PTM	Disulfide bond Glycoprotein Zymogen
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Molecular function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 17	17	Potential
	Propeptide	18 – 24	7	Activation peptide Ref.3		PRO_0000028019
	Chain	25 – 260	236	Arginine esterase		PRO_0000028020
Regions
	Domain	25 – 257	233	Peptidase S1
Sites
	Active site	65	1	Charge relay system By similarity
	Active site	119	1	Charge relay system By similarity
	Active site	212	1	Charge relay system By similarity
Amino acid modifications
	Glycosylation	79	1	N-linked (GlcNAc...) Probable
	Disulfide bond	31 ↔ 172		By similarity
	Disulfide bond	50 ↔ 66		By similarity
	Disulfide bond	151 ↔ 218		By similarity
	Disulfide bond	183 ↔ 197		By similarity
	Disulfide bond	208 ↔ 233		By similarity
Experimental info
	Sequence conflict	56	1	N → H in AAA30831. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P09582-1 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 48768B6EF204775A	FASTA	260	28,746
	10 20 30 40 50 60 MWFLALCLAM SLGWTGAEPH FQPRIIGGRE CLKNSQPWQV AVYHNGEFAC GGVLVNPEWV 70 80 90 100 110 120 LTAAHCANSN CEVWLGRHNL SESEDEGQLV QVRKSFIHPL YKTKVPRAVI RPGEDRSHDL 130 140 150 160 170 180 MLLHLEEPAK ITKAVRVMDL PKKEPPLGST CYVSGWGSTD PETIFHPGSL QCVDLKLLSN 190 200 210 220 230 240 NQCAKVYTQK VTKFMLCAGV LEGKKDTCKG DSGGPLICDG ELVGITSWGA TPCGKPQMPS 250 260 LYTRVMPHLM WIKDTMKANT

References

[1]	"Nucleotide sequence of the androgen-dependent arginine esterase mRNA of canine prostate." Chapdelaine P., Ho-Kim M.-A., Tremblay R.R., Dube J.Y. FEBS Lett. 232:187-192(1988) [PubMed: 2835268] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Prostate.
[2]	"Characterization and expression of the prostatic arginine esterase gene, a canine glandular kallikrein." Chapdelaine P., Gauthier E., Ho-Kim M.-A., Bissonnette L., Tremblay R.R., Dube J.Y. DNA Cell Biol. 10:49-59(1991) [PubMed: 1991049] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The major androgen-dependent protease in dog prostate belongs to the kallikrein family: confirmation by partial amino acid sequencing." Lazure C., Leduc R., Seidah N.G., Chretien M., Dube J.Y., Chapdelaine P., Frenette G., Paquin R., Tremblay R.R. FEBS Lett. 175:1-7(1984) [PubMed: 6566614] [Abstract] Cited for: PROTEIN SEQUENCE OF 25-50 AND 108-145. Tissue: Prostate.
[4]	"Androgen regulation of canine prostatic arginine esterase mRNA using cloned cDNA." Chapdelaine P., Potvin C., Ho-Kim M.A., Larouche L., Bellemare G., Tremblay R.T., Dube J.Y. Mol. Cell. Endocrinol. 56:63-70(1988) [PubMed: 3371547] [Abstract] Cited for: NUCLEOTIDE SEQUENCE OF 105-260.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Y00751 mRNA. Translation: CAA68720.1. M63669 Genomic DNA. Translation: AAA30831.1.
PIR	A30981. A37938.
RefSeq	NP_001003284.1.
UniGene	Cfa.3836
3D structure databases
HSSP	HSSP built from PDB template 2PKA based on UniProtKB P00752.
ModBase	Search...
Protein family/group databases
MEROPS	S01.289.
Genome annotation databases
GeneID	403967.
KEGG	cfa:403967.
Phylogenomic databases
HOVERGEN	P09582.
Enzyme and pathway databases
BRENDA	3.4.21.35. 463.
Family and domain databases
InterPro	IPR018114. Peptidase_S1/S6_AS. IPR001254. Peptidase_S1_S6. IPR001314. Peptidase_S1A. [Graphical view]
Pfam	PF00089. Trypsin. 1 hit. [Graphical view]
PRINTS	PR00722. CHYMOTRYPSIN.
SMART	SM00020. Tryp_SPc. 1 hit. [Graphical view]
PROSITE	PS50240. TRYPSIN_DOM. 1 hit. PS00134. TRYPSIN_HIS. 1 hit. PS00135. TRYPSIN_SER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ESTA_CANFA

Accession

Primary (citable) accession number: P09582

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	May 26, 2009
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)