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P09570

- NIFA_AZOVI

UniProt

P09570 - NIFA_AZOVI

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Protein

Nif-specific regulatory protein

Gene

nifA

Organism
Azotobacter vinelandii
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Required for activation of most nif operons, which are directly involved in nitrogen fixation.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi239 – 2468ATPPROSITE-ProRule annotation
Nucleotide bindingi302 – 31110ATPPROSITE-ProRule annotation
DNA bindingi494 – 51320H-T-H motifBy similarityAdd
BLAST

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. sequence-specific DNA binding Source: InterPro
  3. sequence-specific DNA binding transcription factor activity Source: InterPro

GO - Biological processi

  1. nitrogen fixation Source: UniProtKB-KW
  2. phosphorelay signal transduction system Source: UniProtKB-KW
  3. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Nitrogen fixation, Transcription, Transcription regulation, Two-component regulatory system

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nif-specific regulatory protein
Gene namesi
Name:nifA
OrganismiAzotobacter vinelandii
Taxonomic identifieri354 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaeAzotobacter

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 522522Nif-specific regulatory proteinPRO_0000081304Add
BLAST

Interactioni

Subunit structurei

Interacts with sigma-54.

Protein-protein interaction databases

IntActiP09570. 1 interaction.
MINTiMINT-107513.

Structurei

3D structure databases

ProteinModelPortaliP09570.
SMRiP09570. Positions 210-452.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini37 – 178142GAFAdd
BLAST
Domaini211 – 439229Sigma-54 factor interactionPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 184184A domainAdd
BLAST
Regioni440 – 47940Inter-domain linkerAdd
BLAST
Regioni480 – 52243C-terminal DNA-binding domainAdd
BLAST

Sequence similaritiesi

Contains 1 GAF domain.Curated
Contains 1 sigma-54 factor interaction domain.PROSITE-ProRule annotation

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
3.30.450.40. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003018. GAF.
IPR029016. GAF_dom_like.
IPR009057. Homeodomain-like.
IPR002197. HTH_Fis.
IPR010113. Nif-specific_regulatory_prot.
IPR027417. P-loop_NTPase.
IPR002078. Sigma_54_int.
IPR025662. Sigma_54_int_dom_ATP-bd_1.
IPR025943. Sigma_54_int_dom_ATP-bd_2.
IPR025944. Sigma_54_int_dom_CS.
[Graphical view]
PfamiPF01590. GAF. 1 hit.
PF02954. HTH_8. 1 hit.
PF00158. Sigma54_activat. 1 hit.
[Graphical view]
PRINTSiPR01590. HTHFIS.
SMARTiSM00382. AAA. 1 hit.
SM00065. GAF. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF55781. SSF55781. 1 hit.
TIGRFAMsiTIGR01817. nifA. 1 hit.
PROSITEiPS00675. SIGMA54_INTERACT_1. 1 hit.
PS00676. SIGMA54_INTERACT_2. 1 hit.
PS00688. SIGMA54_INTERACT_3. 1 hit.
PS50045. SIGMA54_INTERACT_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P09570-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNATIPQRSA KQNPVELYDL QLQALASIAR TLSREQQIDE LLEQVLAVLH
60 70 80 90 100
NDLGLLHGLV TISDPEHGAL QIGAIHTDSE AVAQACEGVR YRSGEGVIGN
110 120 130 140 150
VLKHGNSVVL GRISADPRFL DRLALYDLEM PFIAVPIKNP EGNTIGVLAA
160 170 180 190 200
QPDCRADEHM PARTRFLEIV ANLLAQTVRL VVNIEDGREA ADERDELRRE
210 220 230 240 250
VRGKYGFENM VVGHTPTMRR VFDQIRRVAK WNSTVLVLGE SGTGKELIAS
260 270 280 290 300
AIHYKSPRAH RPFVRLNCAA LPETLLESEL FGHEKGAFTG AVKQRKGRFE
310 320 330 340 350
QADGGTLFLD EIGEISPMFQ AKLLRVLQEG EFERVGGNQT VRVNVRIVAA
360 370 380 390 400
TNRDLESEVE KGKFREDLYY RLNVMAIRIP PLRERTADIP ELAEFLLGKI
410 420 430 440 450
GRQQGRPLTV TDSAIRLLMS HRWPGNVREL ENCLERSAIM SEDGTITRDV
460 470 480 490 500
VSLTGVDNES PPLAAPLPEV NLADETLDDR ERVIAALEQA GWVQAKAARL
510 520
LGMTPRQIAY RIQTLNIHMR KI
Length:522
Mass (Da):58,123
Last modified:July 1, 1989 - v1
Checksum:i9556A07EE8A7B209
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y00554 Genomic DNA. Translation: CAA68633.1.
J03411 Genomic DNA. Translation: AAA22147.1.
PIRiS01927.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
Y00554 Genomic DNA. Translation: CAA68633.1 .
J03411 Genomic DNA. Translation: AAA22147.1 .
PIRi S01927.

3D structure databases

ProteinModelPortali P09570.
SMRi P09570. Positions 210-452.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

IntActi P09570. 1 interaction.
MINTi MINT-107513.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

Gene3Di 1.10.10.60. 1 hit.
3.30.450.40. 1 hit.
3.40.50.300. 1 hit.
InterProi IPR003593. AAA+_ATPase.
IPR003018. GAF.
IPR029016. GAF_dom_like.
IPR009057. Homeodomain-like.
IPR002197. HTH_Fis.
IPR010113. Nif-specific_regulatory_prot.
IPR027417. P-loop_NTPase.
IPR002078. Sigma_54_int.
IPR025662. Sigma_54_int_dom_ATP-bd_1.
IPR025943. Sigma_54_int_dom_ATP-bd_2.
IPR025944. Sigma_54_int_dom_CS.
[Graphical view ]
Pfami PF01590. GAF. 1 hit.
PF02954. HTH_8. 1 hit.
PF00158. Sigma54_activat. 1 hit.
[Graphical view ]
PRINTSi PR01590. HTHFIS.
SMARTi SM00382. AAA. 1 hit.
SM00065. GAF. 1 hit.
[Graphical view ]
SUPFAMi SSF46689. SSF46689. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF55781. SSF55781. 1 hit.
TIGRFAMsi TIGR01817. nifA. 1 hit.
PROSITEi PS00675. SIGMA54_INTERACT_1. 1 hit.
PS00676. SIGMA54_INTERACT_2. 1 hit.
PS00688. SIGMA54_INTERACT_3. 1 hit.
PS50045. SIGMA54_INTERACT_4. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Nucleotide sequence and mutagenesis of the nifA gene from Azotobacter vinelandii."
    Bennet L.T., Cannon F., Dean D.R.
    Mol. Microbiol. 2:315-321(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 13705 / OP1 / DSM 366 / NCIB 11614 / LMG 3878 / UW.
  2. "Nucleotide sequence and genetic analysis of the nifB-nifQ region from Azotobacter vinelandii."
    Joerger R.D., Bishop P.E.
    J. Bacteriol. 170:1475-1487(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 394-522.

Entry informationi

Entry nameiNIFA_AZOVI
AccessioniPrimary (citable) accession number: P09570
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: October 29, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3