P09570 (NIFA_AZOVI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nif-specific regulatory protein

Gene names

Name:

nifA

Organism

Azotobacter vinelandii

Taxonomic identifier

354 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pseudomonadales › Pseudomonadaceae › Azotobacter group › Azotobacter

Protein attributes

Sequence length	522 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	Required for activation of most nif operons, which are directly involved in nitrogen fixation.
Subunit structure	Interacts with sigma-54.
Sequence similarities	Contains 1 GAF domain. Contains 1 sigma-54 factor interaction domain.

Ontologies

Keywords
Biological process	Nitrogen fixation Transcription Transcription regulation Two-component regulatory system
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Activator
Gene Ontology (GO)
Biological_process	nitrogen fixation Inferred from electronic annotation. Source: UniProtKB-KW phosphorelay signal transduction system Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro sequence-specific DNA binding transcription factor activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 522	522	Nif-specific regulatory protein		PRO_0000081304
Regions
	Domain	37 – 178	142	GAF
	Domain	211 – 439	229	Sigma-54 factor interaction
	Nucleotide binding	239 – 246	8	ATP Potential
	Nucleotide binding	302 – 311	10	ATP Potential
	DNA binding	494 – 513	20	H-T-H motif By similarity
	Region	1 – 184	184	A domain
	Region	440 – 479	40	Inter-domain linker
	Region	480 – 522	43	C-terminal DNA-binding domain

Sequences

	Sequence		Length	Mass (Da)
	P09570 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 9556A07EE8A7B209	FASTA	522	58,123
	10 20 30 40 50 60 MNATIPQRSA KQNPVELYDL QLQALASIAR TLSREQQIDE LLEQVLAVLH NDLGLLHGLV 70 80 90 100 110 120 TISDPEHGAL QIGAIHTDSE AVAQACEGVR YRSGEGVIGN VLKHGNSVVL GRISADPRFL 130 140 150 160 170 180 DRLALYDLEM PFIAVPIKNP EGNTIGVLAA QPDCRADEHM PARTRFLEIV ANLLAQTVRL 190 200 210 220 230 240 VVNIEDGREA ADERDELRRE VRGKYGFENM VVGHTPTMRR VFDQIRRVAK WNSTVLVLGE 250 260 270 280 290 300 SGTGKELIAS AIHYKSPRAH RPFVRLNCAA LPETLLESEL FGHEKGAFTG AVKQRKGRFE 310 320 330 340 350 360 QADGGTLFLD EIGEISPMFQ AKLLRVLQEG EFERVGGNQT VRVNVRIVAA TNRDLESEVE 370 380 390 400 410 420 KGKFREDLYY RLNVMAIRIP PLRERTADIP ELAEFLLGKI GRQQGRPLTV TDSAIRLLMS 430 440 450 460 470 480 HRWPGNVREL ENCLERSAIM SEDGTITRDV VSLTGVDNES PPLAAPLPEV NLADETLDDR 490 500 510 520 ERVIAALEQA GWVQAKAARL LGMTPRQIAY RIQTLNIHMR KI

References

[1]	"Nucleotide sequence and mutagenesis of the nifA gene from Azotobacter vinelandii." Bennet L.T., Cannon F., Dean D.R. Mol. Microbiol. 2:315-321(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 13705 / OP1 / DSM 366 / NCIB 11614 / LMG 3878 / UW.
[2]	"Nucleotide sequence and genetic analysis of the nifB-nifQ region from Azotobacter vinelandii." Joerger R.D., Bishop P.E. J. Bacteriol. 170:1475-1487(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 394-522.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Y00554 Genomic DNA. Translation: CAA68633.1. J03411 Genomic DNA. Translation: AAA22147.1.
PIR	S01927.
3D structure databases
ProteinModelPortal	P09570.
SMR	P09570. Positions 210-452.
ModBase	Search...
Protein-protein interaction databases
IntAct	P09570. 1 interaction.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	1.10.10.60. 1 hit.
InterPro	IPR003593. AAA+_ATPase. IPR003018. GAF. IPR009057. Homeodomain-like. IPR002197. HTH_Fis. IPR010113. Nif-specific_regulatory_prot. IPR002078. Sigma_54_int. IPR025662. Sigma_54_int_dom_ATP-bd_1. IPR025943. Sigma_54_int_dom_ATP-bd_2. IPR025944. Sigma_54_int_dom_CS. [Graphical view]
Pfam	PF01590. GAF. 1 hit. PF02954. HTH_8. 1 hit. PF00158. Sigma54_activat. 1 hit. [Graphical view]
PRINTS	PR01590. HTHFIS.
SMART	SM00382. AAA. 1 hit. SM00065. GAF. 1 hit. [Graphical view]
SUPFAM	SSF46689. Homeodomain_like. 1 hit.
TIGRFAMs	TIGR01817. nifA. 1 hit.
PROSITE	PS00675. SIGMA54_INTERACT_1. 1 hit. PS00676. SIGMA54_INTERACT_2. 1 hit. PS00688. SIGMA54_INTERACT_3. 1 hit. PS50045. SIGMA54_INTERACT_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NIFA_AZOVI

Accession

Primary (citable) accession number: P09570

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	April 3, 2013
This is version 90 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program