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P09570

- NIFA_AZOVI

UniProt

P09570 - NIFA_AZOVI

Protein

Nif-specific regulatory protein

Gene

nifA

Organism
Azotobacter vinelandii
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (01 Jul 1989)
      Previous versions | rss
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    • Comment

    Functioni

    Required for activation of most nif operons, which are directly involved in nitrogen fixation.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi239 – 2468ATPPROSITE-ProRule annotation
    Nucleotide bindingi302 – 31110ATPPROSITE-ProRule annotation
    DNA bindingi494 – 51320H-T-H motifBy similarityAdd
    BLAST

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. nucleoside-triphosphatase activity Source: InterPro
    3. sequence-specific DNA binding Source: InterPro
    4. sequence-specific DNA binding transcription factor activity Source: InterPro

    GO - Biological processi

    1. nitrogen fixation Source: UniProtKB-KW
    2. phosphorelay signal transduction system Source: UniProtKB-KW
    3. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator

    Keywords - Biological processi

    Nitrogen fixation, Transcription, Transcription regulation, Two-component regulatory system

    Keywords - Ligandi

    ATP-binding, DNA-binding, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Nif-specific regulatory protein
    Gene namesi
    Name:nifA
    OrganismiAzotobacter vinelandii
    Taxonomic identifieri354 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaeAzotobacter

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 522522Nif-specific regulatory proteinPRO_0000081304Add
    BLAST

    Interactioni

    Subunit structurei

    Interacts with sigma-54.

    Protein-protein interaction databases

    IntActiP09570. 1 interaction.
    MINTiMINT-107513.

    Structurei

    3D structure databases

    ProteinModelPortaliP09570.
    SMRiP09570. Positions 210-452.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini37 – 178142GAFAdd
    BLAST
    Domaini211 – 439229Sigma-54 factor interactionPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 184184A domainAdd
    BLAST
    Regioni440 – 47940Inter-domain linkerAdd
    BLAST
    Regioni480 – 52243C-terminal DNA-binding domainAdd
    BLAST

    Sequence similaritiesi

    Contains 1 GAF domain.Curated
    Contains 1 sigma-54 factor interaction domain.PROSITE-ProRule annotation

    Family and domain databases

    Gene3Di1.10.10.60. 1 hit.
    3.30.450.40. 1 hit.
    3.40.50.300. 1 hit.
    InterProiIPR003593. AAA+_ATPase.
    IPR003018. GAF.
    IPR029016. GAF_dom_like.
    IPR009057. Homeodomain-like.
    IPR002197. HTH_Fis.
    IPR010113. Nif-specific_regulatory_prot.
    IPR027417. P-loop_NTPase.
    IPR002078. Sigma_54_int.
    IPR025662. Sigma_54_int_dom_ATP-bd_1.
    IPR025943. Sigma_54_int_dom_ATP-bd_2.
    IPR025944. Sigma_54_int_dom_CS.
    [Graphical view]
    PfamiPF01590. GAF. 1 hit.
    PF02954. HTH_8. 1 hit.
    PF00158. Sigma54_activat. 1 hit.
    [Graphical view]
    PRINTSiPR01590. HTHFIS.
    SMARTiSM00382. AAA. 1 hit.
    SM00065. GAF. 1 hit.
    [Graphical view]
    SUPFAMiSSF46689. SSF46689. 1 hit.
    SSF52540. SSF52540. 1 hit.
    SSF55781. SSF55781. 1 hit.
    TIGRFAMsiTIGR01817. nifA. 1 hit.
    PROSITEiPS00675. SIGMA54_INTERACT_1. 1 hit.
    PS00676. SIGMA54_INTERACT_2. 1 hit.
    PS00688. SIGMA54_INTERACT_3. 1 hit.
    PS50045. SIGMA54_INTERACT_4. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P09570-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNATIPQRSA KQNPVELYDL QLQALASIAR TLSREQQIDE LLEQVLAVLH    50
    NDLGLLHGLV TISDPEHGAL QIGAIHTDSE AVAQACEGVR YRSGEGVIGN 100
    VLKHGNSVVL GRISADPRFL DRLALYDLEM PFIAVPIKNP EGNTIGVLAA 150
    QPDCRADEHM PARTRFLEIV ANLLAQTVRL VVNIEDGREA ADERDELRRE 200
    VRGKYGFENM VVGHTPTMRR VFDQIRRVAK WNSTVLVLGE SGTGKELIAS 250
    AIHYKSPRAH RPFVRLNCAA LPETLLESEL FGHEKGAFTG AVKQRKGRFE 300
    QADGGTLFLD EIGEISPMFQ AKLLRVLQEG EFERVGGNQT VRVNVRIVAA 350
    TNRDLESEVE KGKFREDLYY RLNVMAIRIP PLRERTADIP ELAEFLLGKI 400
    GRQQGRPLTV TDSAIRLLMS HRWPGNVREL ENCLERSAIM SEDGTITRDV 450
    VSLTGVDNES PPLAAPLPEV NLADETLDDR ERVIAALEQA GWVQAKAARL 500
    LGMTPRQIAY RIQTLNIHMR KI 522
    Length:522
    Mass (Da):58,123
    Last modified:July 1, 1989 - v1
    Checksum:i9556A07EE8A7B209
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y00554 Genomic DNA. Translation: CAA68633.1.
    J03411 Genomic DNA. Translation: AAA22147.1.
    PIRiS01927.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Y00554 Genomic DNA. Translation: CAA68633.1 .
    J03411 Genomic DNA. Translation: AAA22147.1 .
    PIRi S01927.

    3D structure databases

    ProteinModelPortali P09570.
    SMRi P09570. Positions 210-452.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P09570. 1 interaction.
    MINTi MINT-107513.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 1.10.10.60. 1 hit.
    3.30.450.40. 1 hit.
    3.40.50.300. 1 hit.
    InterProi IPR003593. AAA+_ATPase.
    IPR003018. GAF.
    IPR029016. GAF_dom_like.
    IPR009057. Homeodomain-like.
    IPR002197. HTH_Fis.
    IPR010113. Nif-specific_regulatory_prot.
    IPR027417. P-loop_NTPase.
    IPR002078. Sigma_54_int.
    IPR025662. Sigma_54_int_dom_ATP-bd_1.
    IPR025943. Sigma_54_int_dom_ATP-bd_2.
    IPR025944. Sigma_54_int_dom_CS.
    [Graphical view ]
    Pfami PF01590. GAF. 1 hit.
    PF02954. HTH_8. 1 hit.
    PF00158. Sigma54_activat. 1 hit.
    [Graphical view ]
    PRINTSi PR01590. HTHFIS.
    SMARTi SM00382. AAA. 1 hit.
    SM00065. GAF. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46689. SSF46689. 1 hit.
    SSF52540. SSF52540. 1 hit.
    SSF55781. SSF55781. 1 hit.
    TIGRFAMsi TIGR01817. nifA. 1 hit.
    PROSITEi PS00675. SIGMA54_INTERACT_1. 1 hit.
    PS00676. SIGMA54_INTERACT_2. 1 hit.
    PS00688. SIGMA54_INTERACT_3. 1 hit.
    PS50045. SIGMA54_INTERACT_4. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence and mutagenesis of the nifA gene from Azotobacter vinelandii."
      Bennet L.T., Cannon F., Dean D.R.
      Mol. Microbiol. 2:315-321(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 13705 / OP1 / DSM 366 / NCIB 11614 / LMG 3878 / UW.
    2. "Nucleotide sequence and genetic analysis of the nifB-nifQ region from Azotobacter vinelandii."
      Joerger R.D., Bishop P.E.
      J. Bacteriol. 170:1475-1487(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 394-522.

    Entry informationi

    Entry nameiNIFA_AZOVI
    AccessioniPrimary (citable) accession number: P09570
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3