Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Nif-specific regulatory protein

Gene

nifA

Organism
Azotobacter vinelandii
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Required for activation of most nif operons, which are directly involved in nitrogen fixation.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi239 – 246ATPPROSITE-ProRule annotation8
Nucleotide bindingi302 – 311ATPPROSITE-ProRule annotation10
DNA bindingi494 – 513H-T-H motifBy similarityAdd BLAST20

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Nitrogen fixation, Transcription, Transcription regulation, Two-component regulatory system

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Nif-specific regulatory protein
Gene namesi
Name:nifA
OrganismiAzotobacter vinelandii
Taxonomic identifieri354 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaeAzotobacter

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000813041 – 522Nif-specific regulatory proteinAdd BLAST522

Interactioni

Subunit structurei

Interacts with sigma-54.

Protein-protein interaction databases

IntActiP09570. 1 interactor.
MINTiMINT-107513.
STRINGi322710.Avin_51000.

Structurei

3D structure databases

ProteinModelPortaliP09570.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini37 – 178GAFAdd BLAST142
Domaini211 – 439Sigma-54 factor interactionPROSITE-ProRule annotationAdd BLAST229

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 184A domainAdd BLAST184
Regioni440 – 479Inter-domain linkerAdd BLAST40
Regioni480 – 522C-terminal DNA-binding domainAdd BLAST43

Sequence similaritiesi

Contains 1 GAF domain.Curated
Contains 1 sigma-54 factor interaction domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108JUA. Bacteria.
COG3604. LUCA.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
3.30.450.40. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003018. GAF.
IPR029016. GAF_dom-like.
IPR009057. Homeodomain-like.
IPR002197. HTH_Fis.
IPR010113. Nif-specific_regulatory_prot.
IPR027417. P-loop_NTPase.
IPR002078. Sigma_54_int.
IPR025662. Sigma_54_int_dom_ATP-bd_1.
IPR025943. Sigma_54_int_dom_ATP-bd_2.
IPR025944. Sigma_54_int_dom_CS.
[Graphical view]
PfamiPF13185. GAF_2. 1 hit.
PF02954. HTH_8. 1 hit.
PF00158. Sigma54_activat. 1 hit.
[Graphical view]
PRINTSiPR01590. HTHFIS.
SMARTiSM00382. AAA. 1 hit.
SM00065. GAF. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF55781. SSF55781. 1 hit.
TIGRFAMsiTIGR01817. nifA. 1 hit.
PROSITEiPS00675. SIGMA54_INTERACT_1. 1 hit.
PS00676. SIGMA54_INTERACT_2. 1 hit.
PS00688. SIGMA54_INTERACT_3. 1 hit.
PS50045. SIGMA54_INTERACT_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P09570-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNATIPQRSA KQNPVELYDL QLQALASIAR TLSREQQIDE LLEQVLAVLH
60 70 80 90 100
NDLGLLHGLV TISDPEHGAL QIGAIHTDSE AVAQACEGVR YRSGEGVIGN
110 120 130 140 150
VLKHGNSVVL GRISADPRFL DRLALYDLEM PFIAVPIKNP EGNTIGVLAA
160 170 180 190 200
QPDCRADEHM PARTRFLEIV ANLLAQTVRL VVNIEDGREA ADERDELRRE
210 220 230 240 250
VRGKYGFENM VVGHTPTMRR VFDQIRRVAK WNSTVLVLGE SGTGKELIAS
260 270 280 290 300
AIHYKSPRAH RPFVRLNCAA LPETLLESEL FGHEKGAFTG AVKQRKGRFE
310 320 330 340 350
QADGGTLFLD EIGEISPMFQ AKLLRVLQEG EFERVGGNQT VRVNVRIVAA
360 370 380 390 400
TNRDLESEVE KGKFREDLYY RLNVMAIRIP PLRERTADIP ELAEFLLGKI
410 420 430 440 450
GRQQGRPLTV TDSAIRLLMS HRWPGNVREL ENCLERSAIM SEDGTITRDV
460 470 480 490 500
VSLTGVDNES PPLAAPLPEV NLADETLDDR ERVIAALEQA GWVQAKAARL
510 520
LGMTPRQIAY RIQTLNIHMR KI
Length:522
Mass (Da):58,123
Last modified:July 1, 1989 - v1
Checksum:i9556A07EE8A7B209
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00554 Genomic DNA. Translation: CAA68633.1.
J03411 Genomic DNA. Translation: AAA22147.1.
PIRiS01927.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
Y00554 Genomic DNA. Translation: CAA68633.1.
J03411 Genomic DNA. Translation: AAA22147.1.
PIRiS01927.

3D structure databases

ProteinModelPortaliP09570.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP09570. 1 interactor.
MINTiMINT-107513.
STRINGi322710.Avin_51000.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4108JUA. Bacteria.
COG3604. LUCA.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
3.30.450.40. 1 hit.
3.40.50.300. 1 hit.
InterProiIPR003593. AAA+_ATPase.
IPR003018. GAF.
IPR029016. GAF_dom-like.
IPR009057. Homeodomain-like.
IPR002197. HTH_Fis.
IPR010113. Nif-specific_regulatory_prot.
IPR027417. P-loop_NTPase.
IPR002078. Sigma_54_int.
IPR025662. Sigma_54_int_dom_ATP-bd_1.
IPR025943. Sigma_54_int_dom_ATP-bd_2.
IPR025944. Sigma_54_int_dom_CS.
[Graphical view]
PfamiPF13185. GAF_2. 1 hit.
PF02954. HTH_8. 1 hit.
PF00158. Sigma54_activat. 1 hit.
[Graphical view]
PRINTSiPR01590. HTHFIS.
SMARTiSM00382. AAA. 1 hit.
SM00065. GAF. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF52540. SSF52540. 1 hit.
SSF55781. SSF55781. 1 hit.
TIGRFAMsiTIGR01817. nifA. 1 hit.
PROSITEiPS00675. SIGMA54_INTERACT_1. 1 hit.
PS00676. SIGMA54_INTERACT_2. 1 hit.
PS00688. SIGMA54_INTERACT_3. 1 hit.
PS50045. SIGMA54_INTERACT_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNIFA_AZOVI
AccessioniPrimary (citable) accession number: P09570
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: October 5, 2016
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.