P09569 (ADEN_ADE12) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Adenain EC=3.4.22.39 Alternative name(s): Endoprotease Late L3 23 kDa protein
Organism	Human adenovirus A serotype 12 (HAdV-12) (Human adenovirus 12)
Taxonomic identifier	28282 [NCBI]
Taxonomic lineage	Viruses › dsDNA viruses, no RNA stage › Adenoviridae › Mastadenovirus › Human adenovirus A
Virus host	Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	206 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Thiol protease cleaving specific Gly-Ala peptides in a number of viral precursor proteins (terminal, IIIa, VI, VII, VIII, IIa and mu) which are required for virion maturation. Also cleaves host cells cytoskeletal keratins K7 and K18.
Catalytic activity	Cleaves proteins of the adenovirus and its host cell at two consensus sites: -Yaa-Xaa-Gly-Gly-|-Xaa- and -Yaa-Xaa-Gly-Xaa-|-Gly- (in which Yaa is Met, Ile or Leu, and Xaa is any amino acid).
Sequence similarities	Belongs to the peptidase C5 family.

Ontologies

Keywords
Developmental stage	Late protein
Molecular function	Hydrolase Protease Thiol protease
PTM	Autocatalytic cleavage
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cysteine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 206	206	Adenain		PRO_0000218028
Sites
	Active site	54	1	By similarity
	Active site	71	1	By similarity
	Active site	122	1	By similarity
	Site	46 – 47	2	Cleavage; by autolysis Potential
Experimental info
	Sequence conflict	180	1	N → S in CAA30501. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P09569 [UniParc]. Last modified June 1, 1994. Version 2. Checksum: 6876ED52765E7357	FASTA	206	23,482
	10 20 30 40 50 60 MGSSEQELTA IVRDLGCGPY FLGTFDKRFP GFVSRDRLSC AIVNTAGRET GGVHWLAFGW 70 80 90 100 110 120 NPKSHTCYLF DPFGFSDQRL KQIYQFEYES LLRRSALAAT KDRCVTLEKS TQTVQGPFSA 130 140 150 160 170 180 ACGLFCCMFL HAFTHWPDHP MDKNPTMDLL TGVPNCMLQS PQVVGTLQRN QNELYKFLNN 190 200 LSPYFRHNRE RIEKATSFTK MQNGLK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The primary structure of human adenovirus type 12 protease." Weber J.M., Houde A. Nucleic Acids Res. 16:7195-7195(1988) [PubMed: 3043380] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Pereira 1131.
[2]	"Nucleotide sequence of human adenovirus type 12 DNA: comparative functional analysis." Sprengel J., Schmitz B., Heuss-Neitzel D., Zock C., Doerfler W. J. Virol. 68:379-389(1994) [PubMed: 8254750] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X07655 Genomic DNA. Translation: CAA30501.1. X73487 Genomic DNA. Translation: CAA51892.1.
PIR	W2AD12. S01731.
RefSeq	NP_040925.1. NC_001460.1.
3D structure databases
ProteinModelPortal	P09569.
SMR	P09569. Positions 1-203.
ModBase	Search...
Protein family/group databases
MEROPS	C05.001.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1460858.
Phylogenomic databases
ProtClustDB	CLSP2513881.
Family and domain databases
InterPro	IPR000855. Peptidase_C5. [Graphical view]
Gene3D	G3DSA:3.40.395.10. Peptidase_C5. 1 hit.
Pfam	PF00770. Peptidase_C5. 1 hit. [Graphical view]
PIRSF	PIRSF001218. Protease_ADV. 1 hit.
PRINTS	PR00703. ADVENDOPTASE.
ProDom	PD003705. Peptidase_C5. 1 hit. [Graphical view] [Entries sharing at least one domain]
ProtoNet	Search...

Entry information

Entry name

ADEN_ADE12

Accession

Primary (citable) accession number: P09569

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	June 1, 1994
Last modified:	November 16, 2011
This is version 62 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program