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P09560

- RAF1_XENLA

UniProt

P09560 - RAF1_XENLA

Protein

RAF proto-oncogene serine/threonine-protein kinase

Gene

raf1

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 107 (01 Oct 2014)
      Sequence version 1 (01 Jul 1989)
      Previous versions | rss
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    Functioni

    Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2) By similarity.By similarity

    Catalytic activityi

    ATP + a protein = ADP + a phosphoprotein.

    Cofactori

    Binds 2 zinc ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi138 – 1381Zinc 1By similarity
    Metal bindingi151 – 1511Zinc 2By similarity
    Metal bindingi154 – 1541Zinc 2By similarity
    Metal bindingi164 – 1641Zinc 1By similarity
    Metal bindingi167 – 1671Zinc 1By similarity
    Metal bindingi172 – 1721Zinc 2By similarity
    Metal bindingi175 – 1751Zinc 2By similarity
    Metal bindingi183 – 1831Zinc 1By similarity
    Binding sitei366 – 3661ATPPROSITE-ProRule annotation
    Active sitei459 – 4591Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri137 – 18347Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd
    BLAST
    Nucleotide bindingi346 – 3549ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-KW
    3. protein serine/threonine kinase activity Source: UniProtKB-KW
    4. receptor signaling protein activity Source: InterPro

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BRENDAi2.7.10.2. 6726.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    RAF proto-oncogene serine/threonine-protein kinase (EC:2.7.11.1)
    Alternative name(s):
    C-RAF
    Gene namesi
    Name:raf1
    Synonyms:raf
    OrganismiXenopus laevis (African clawed frog)
    Taxonomic identifieri8355 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

    Organism-specific databases

    XenbaseiXB-GENE-6053397. raf1.

    Subcellular locationi

    Cytoplasm By similarity. Cell membrane By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB
    2. plasma membrane Source: UniProtKB

    Keywords - Cellular componenti

    Cell membrane, Cytoplasm, Membrane

    Pathology & Biotechi

    Keywords - Diseasei

    Proto-oncogene

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 638638RAF proto-oncogene serine/threonine-protein kinasePRO_0000086600Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei43 – 431PhosphoserineBy similarity
    Modified residuei257 – 2571PhosphoserineBy similarity
    Modified residuei266 – 2661Phosphothreonine; by autocatalysisBy similarity
    Modified residuei329 – 3291PhosphoserineBy similarity
    Modified residuei490 – 4901PhosphoserineBy similarity

    Post-translational modificationi

    Phosphorylation at Ser-257 inactivates kinase activity. Dephosphorylation of Ser-257 by a complex containing protein phosphatase 1 relieves inactivation, leading to stimulate RAF1 activity By similarity.By similarity

    Keywords - PTMi

    Phosphoprotein

    Interactioni

    Protein-protein interaction databases

    DIPiDIP-1075N.

    Structurei

    3D structure databases

    ProteinModelPortaliP09560.
    SMRiP09560. Positions 55-131, 135-186, 333-606.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini56 – 13075RBDPROSITE-ProRule annotationAdd
    BLAST
    Domaini340 – 600261Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 phorbol-ester/DAG-type zinc finger.PROSITE-ProRule annotation
    Contains 1 protein kinase domain.PROSITE-ProRule annotation
    Contains 1 RBD (Ras-binding) domain.PROSITE-ProRule annotation

    Zinc finger

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Zinc fingeri137 – 18347Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd
    BLAST

    Keywords - Domaini

    Zinc-finger

    Phylogenomic databases

    HOVERGENiHBG001886.

    Family and domain databases

    InterProiIPR011009. Kinase-like_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR003116. Raf-like_ras-bd.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR008271. Ser/Thr_kinase_AS.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view]
    PfamiPF00130. C1_1. 1 hit.
    PF07714. Pkinase_Tyr. 1 hit.
    PF02196. RBD. 1 hit.
    [Graphical view]
    SMARTiSM00109. C1. 1 hit.
    SM00455. RBD. 1 hit.
    [Graphical view]
    SUPFAMiSSF54236. SSF54236. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    PS50898. RBD. 1 hit.
    PS00479. ZF_DAG_PE_1. 1 hit.
    PS50081. ZF_DAG_PE_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P09560-1 [UniParc]FASTAAdd to Basket

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    MEHIQGAWKT LSNGFGFKES VFEGSSCMSP TIVHQFGYQR RASDDGKLSD    50
    TSKTSSTMRV YLPNKQRTVV NVRSGMSLHD CLMKSLKVRG LQPECCAVFR 100
    LIQDPKGKLR LDWNTDAMSL VGAELQVDFL DHVPLTTHNF VRKTFLKLAF 150
    CDICQKFLLN AFRCQTCGYK FHEHCSTKVP TMCVDWSNIR QLLLFPNPNN 200
    IEGGSHTLPS LTMRRIGESV RIPVSSQQRY STPHPFSFST PSPVSECSLS 250
    QRQRSTSTPN VHMVSTTMAV DSRVIEDALR SHSESGSPNN LSPTGWSNAK 300
    APAPTHREKA ASSTGQEKNK IRARGQRDSS YYWEIIASEV MLSSRIGSGS 350
    FGTVYKGKWH GDVAVKILKV TDPTPEQLQA FRNEVAVLRK TRHVNILLFM 400
    GYMTKDNLAI VTQWCEGSSL YYHLHVLDTK FQMFQLIDIA RQTAQGMDYL 450
    HAKNIIHRDM KSNNIFLHEG LTVKIGDFGL ATVKTRWSGS QQVEQLTGSI 500
    LWMAPEVIRM QDNNPFSFQS DVYSYGIVLY ELMTGELPYS HIRDRDQIIF 550
    LVGRGGVVPD LSKLYKNCPK AMKRLVADSI KKLRDERPLF PQILSSIELL 600
    QHSLPKINRS ALEPSLHRAA HTEDISSCAL TSTRLPVF 638
    Length:638
    Mass (Da):71,960
    Last modified:July 1, 1989 - v1
    Checksum:i1FF352BFFBF528DF
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti309 – 3091K → R in AAB20707. (PubMed:1721855)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X12948 mRNA. Translation: CAA31407.1.
    S74063 mRNA. Translation: AAB20707.1.
    PIRiS01930. TVXLRF.
    UniGeneiXl.5697.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X12948 mRNA. Translation: CAA31407.1 .
    S74063 mRNA. Translation: AAB20707.1 .
    PIRi S01930. TVXLRF.
    UniGenei Xl.5697.

    3D structure databases

    ProteinModelPortali P09560.
    SMRi P09560. Positions 55-131, 135-186, 333-606.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-1075N.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    Xenbasei XB-GENE-6053397. raf1.

    Phylogenomic databases

    HOVERGENi HBG001886.

    Enzyme and pathway databases

    BRENDAi 2.7.10.2. 6726.

    Family and domain databases

    InterProi IPR011009. Kinase-like_dom.
    IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR003116. Raf-like_ras-bd.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR008271. Ser/Thr_kinase_AS.
    IPR029071. Ubiquitin-rel_dom.
    [Graphical view ]
    Pfami PF00130. C1_1. 1 hit.
    PF07714. Pkinase_Tyr. 1 hit.
    PF02196. RBD. 1 hit.
    [Graphical view ]
    SMARTi SM00109. C1. 1 hit.
    SM00455. RBD. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54236. SSF54236. 1 hit.
    SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    PS50898. RBD. 1 hit.
    PS00479. ZF_DAG_PE_1. 1 hit.
    PS50081. ZF_DAG_PE_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Oocyte.
    2. "Xenopus c-raf proto-oncogene: cloning and expression during oogenesis and early development."
      le Guellec R., Couturier A., le Guellec K., Paris J., le Fur N., Philippe M.
      Biol. Cell 72:39-45(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].

    Entry informationi

    Entry nameiRAF1_XENLA
    AccessioniPrimary (citable) accession number: P09560
    Secondary accession number(s): Q91390
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 107 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3