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P09560 (RAF1_XENLA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
RAF proto-oncogene serine/threonine-protein kinase

EC=2.7.11.1
Alternative name(s):
C-RAF
Gene names
Name:raf1
Synonyms:raf
OrganismXenopus laevis (African clawed frog)
Taxonomic identifier8355 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Protein attributes

Sequence length638 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2) By similarity.

Catalytic activity

ATP + a protein = ADP + a phosphoprotein.

Cofactor

Binds 2 zinc ions per subunit By similarity.

Subcellular location

Cytoplasm By similarity. Cell membrane By similarity.

Post-translational modification

Phosphorylation at Ser-257 inactivates kinase activity. Dephosphorylation of Ser-257 by a complex containing protein phosphatase 1 relieves inactivation, leading to stimulate RAF1 activity By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.

Contains 1 phorbol-ester/DAG-type zinc finger.

Contains 1 protein kinase domain.

Contains 1 RBD (Ras-binding) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 638638RAF proto-oncogene serine/threonine-protein kinase
PRO_0000086600

Regions

Domain56 – 13075RBD
Domain340 – 600261Protein kinase
Zinc finger137 – 18347Phorbol-ester/DAG-type
Nucleotide binding346 – 3549ATP By similarity

Sites

Active site4591Proton acceptor By similarity
Metal binding1381Zinc 1 By similarity
Metal binding1511Zinc 2 By similarity
Metal binding1541Zinc 2 By similarity
Metal binding1641Zinc 1 By similarity
Metal binding1671Zinc 1 By similarity
Metal binding1721Zinc 2 By similarity
Metal binding1751Zinc 2 By similarity
Metal binding1831Zinc 1 By similarity
Binding site3661ATP By similarity

Amino acid modifications

Modified residue431Phosphoserine By similarity
Modified residue2571Phosphoserine By similarity
Modified residue2661Phosphothreonine; by autocatalysis By similarity
Modified residue3291Phosphoserine By similarity
Modified residue4901Phosphoserine By similarity

Experimental info

Sequence conflict3091K → R in AAB20707. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P09560 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 1FF352BFFBF528DF

FASTA63871,960
        10         20         30         40         50         60 
MEHIQGAWKT LSNGFGFKES VFEGSSCMSP TIVHQFGYQR RASDDGKLSD TSKTSSTMRV 

        70         80         90        100        110        120 
YLPNKQRTVV NVRSGMSLHD CLMKSLKVRG LQPECCAVFR LIQDPKGKLR LDWNTDAMSL 

       130        140        150        160        170        180 
VGAELQVDFL DHVPLTTHNF VRKTFLKLAF CDICQKFLLN AFRCQTCGYK FHEHCSTKVP 

       190        200        210        220        230        240 
TMCVDWSNIR QLLLFPNPNN IEGGSHTLPS LTMRRIGESV RIPVSSQQRY STPHPFSFST 

       250        260        270        280        290        300 
PSPVSECSLS QRQRSTSTPN VHMVSTTMAV DSRVIEDALR SHSESGSPNN LSPTGWSNAK 

       310        320        330        340        350        360 
APAPTHREKA ASSTGQEKNK IRARGQRDSS YYWEIIASEV MLSSRIGSGS FGTVYKGKWH 

       370        380        390        400        410        420 
GDVAVKILKV TDPTPEQLQA FRNEVAVLRK TRHVNILLFM GYMTKDNLAI VTQWCEGSSL 

       430        440        450        460        470        480 
YYHLHVLDTK FQMFQLIDIA RQTAQGMDYL HAKNIIHRDM KSNNIFLHEG LTVKIGDFGL 

       490        500        510        520        530        540 
ATVKTRWSGS QQVEQLTGSI LWMAPEVIRM QDNNPFSFQS DVYSYGIVLY ELMTGELPYS 

       550        560        570        580        590        600 
HIRDRDQIIF LVGRGGVVPD LSKLYKNCPK AMKRLVADSI KKLRDERPLF PQILSSIELL 

       610        620        630 
QHSLPKINRS ALEPSLHRAA HTEDISSCAL TSTRLPVF 

« Hide

References

[1]"Nucleotide sequence of Xenopus C-raf coding region."
le Guellec R., le Guellec K., Paris J., Philippe M.
Nucleic Acids Res. 16:10357-10357(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Oocyte.
[2]"Xenopus c-raf proto-oncogene: cloning and expression during oogenesis and early development."
le Guellec R., Couturier A., le Guellec K., Paris J., le Fur N., Philippe M.
Biol. Cell 72:39-45(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X12948 mRNA. Translation: CAA31407.1.
S74063 mRNA. Translation: AAB20707.1.
PIRTVXLRF. S01930.
UniGeneXl.5697.

3D structure databases

ProteinModelPortalP09560.
SMRP09560. Positions 55-131, 135-186, 333-606.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-1075N.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

XenbaseXB-GENE-6053397. raf1.

Phylogenomic databases

HOVERGENHBG001886.

Enzyme and pathway databases

BRENDA2.7.10.2. 6726.

Family and domain databases

InterProIPR011009. Kinase-like_dom.
IPR002219. Prot_Kinase_C-like_PE/DAG-bd.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR003116. Raf-like_ras-bd.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamPF00130. C1_1. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
PF02196. RBD. 1 hit.
[Graphical view]
SMARTSM00109. C1. 1 hit.
SM00455. RBD. 1 hit.
[Graphical view]
SUPFAMSSF56112. SSF56112. 1 hit.
PROSITEPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50898. RBD. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRAF1_XENLA
AccessionPrimary (citable) accession number: P09560
Secondary accession number(s): Q91390
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: February 19, 2014
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families