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P09560

- RAF1_XENLA

UniProt

P09560 - RAF1_XENLA

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Protein

RAF proto-oncogene serine/threonine-protein kinase

Gene

raf1

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli

Functioni

Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2) (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Binds 2 zinc ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi138 – 1381Zinc 1By similarity
Metal bindingi151 – 1511Zinc 2By similarity
Metal bindingi154 – 1541Zinc 2By similarity
Metal bindingi164 – 1641Zinc 1By similarity
Metal bindingi167 – 1671Zinc 1By similarity
Metal bindingi172 – 1721Zinc 2By similarity
Metal bindingi175 – 1751Zinc 2By similarity
Metal bindingi183 – 1831Zinc 1By similarity
Binding sitei366 – 3661ATPPROSITE-ProRule annotation
Active sitei459 – 4591Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri137 – 18347Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd
BLAST
Nucleotide bindingi346 – 3549ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: UniProtKB-KW
  3. protein serine/threonine kinase activity Source: UniProtKB-KW
  4. receptor signaling protein activity Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi2.7.10.2. 6726.

Names & Taxonomyi

Protein namesi
Recommended name:
RAF proto-oncogene serine/threonine-protein kinase (EC:2.7.11.1)
Alternative name(s):
C-RAF
Gene namesi
Name:raf1
Synonyms:raf
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Organism-specific databases

XenbaseiXB-GENE-6053397. raf1.

Subcellular locationi

Cytoplasm By similarity. Cell membrane By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB
  2. plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 638638RAF proto-oncogene serine/threonine-protein kinasePRO_0000086600Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei43 – 431PhosphoserineBy similarity
Modified residuei257 – 2571PhosphoserineBy similarity
Modified residuei266 – 2661Phosphothreonine; by autocatalysisBy similarity
Modified residuei329 – 3291PhosphoserineBy similarity
Modified residuei490 – 4901PhosphoserineBy similarity

Post-translational modificationi

Phosphorylation at Ser-257 inactivates kinase activity. Dephosphorylation of Ser-257 by a complex containing protein phosphatase 1 relieves inactivation, leading to stimulate RAF1 activity (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Protein-protein interaction databases

DIPiDIP-1075N.

Structurei

3D structure databases

ProteinModelPortaliP09560.
SMRiP09560. Positions 55-131, 135-186, 333-606.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini56 – 13075RBDPROSITE-ProRule annotationAdd
BLAST
Domaini340 – 600261Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 phorbol-ester/DAG-type zinc finger.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 RBD (Ras-binding) domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Zinc fingeri137 – 18347Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd
BLAST

Keywords - Domaini

Zinc-finger

Phylogenomic databases

HOVERGENiHBG001886.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR002219. PE/DAG-bd.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR003116. Raf-like_ras-bd.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00130. C1_1. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
PF02196. RBD. 1 hit.
[Graphical view]
SMARTiSM00109. C1. 1 hit.
SM00455. RBD. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50898. RBD. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P09560-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MEHIQGAWKT LSNGFGFKES VFEGSSCMSP TIVHQFGYQR RASDDGKLSD
60 70 80 90 100
TSKTSSTMRV YLPNKQRTVV NVRSGMSLHD CLMKSLKVRG LQPECCAVFR
110 120 130 140 150
LIQDPKGKLR LDWNTDAMSL VGAELQVDFL DHVPLTTHNF VRKTFLKLAF
160 170 180 190 200
CDICQKFLLN AFRCQTCGYK FHEHCSTKVP TMCVDWSNIR QLLLFPNPNN
210 220 230 240 250
IEGGSHTLPS LTMRRIGESV RIPVSSQQRY STPHPFSFST PSPVSECSLS
260 270 280 290 300
QRQRSTSTPN VHMVSTTMAV DSRVIEDALR SHSESGSPNN LSPTGWSNAK
310 320 330 340 350
APAPTHREKA ASSTGQEKNK IRARGQRDSS YYWEIIASEV MLSSRIGSGS
360 370 380 390 400
FGTVYKGKWH GDVAVKILKV TDPTPEQLQA FRNEVAVLRK TRHVNILLFM
410 420 430 440 450
GYMTKDNLAI VTQWCEGSSL YYHLHVLDTK FQMFQLIDIA RQTAQGMDYL
460 470 480 490 500
HAKNIIHRDM KSNNIFLHEG LTVKIGDFGL ATVKTRWSGS QQVEQLTGSI
510 520 530 540 550
LWMAPEVIRM QDNNPFSFQS DVYSYGIVLY ELMTGELPYS HIRDRDQIIF
560 570 580 590 600
LVGRGGVVPD LSKLYKNCPK AMKRLVADSI KKLRDERPLF PQILSSIELL
610 620 630
QHSLPKINRS ALEPSLHRAA HTEDISSCAL TSTRLPVF
Length:638
Mass (Da):71,960
Last modified:July 1, 1989 - v1
Checksum:i1FF352BFFBF528DF
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti309 – 3091K → R in AAB20707. (PubMed:1721855)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X12948 mRNA. Translation: CAA31407.1.
S74063 mRNA. Translation: AAB20707.1.
PIRiS01930. TVXLRF.
UniGeneiXl.5697.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X12948 mRNA. Translation: CAA31407.1 .
S74063 mRNA. Translation: AAB20707.1 .
PIRi S01930. TVXLRF.
UniGenei Xl.5697.

3D structure databases

ProteinModelPortali P09560.
SMRi P09560. Positions 55-131, 135-186, 333-606.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-1075N.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

Xenbasei XB-GENE-6053397. raf1.

Phylogenomic databases

HOVERGENi HBG001886.

Enzyme and pathway databases

BRENDAi 2.7.10.2. 6726.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR002219. PE/DAG-bd.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR003116. Raf-like_ras-bd.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR029071. Ubiquitin-rel_dom.
[Graphical view ]
Pfami PF00130. C1_1. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
PF02196. RBD. 1 hit.
[Graphical view ]
SMARTi SM00109. C1. 1 hit.
SM00455. RBD. 1 hit.
[Graphical view ]
SUPFAMi SSF54236. SSF54236. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50898. RBD. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Oocyte.
  2. "Xenopus c-raf proto-oncogene: cloning and expression during oogenesis and early development."
    le Guellec R., Couturier A., le Guellec K., Paris J., le Fur N., Philippe M.
    Biol. Cell 72:39-45(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Entry informationi

Entry nameiRAF1_XENLA
AccessioniPrimary (citable) accession number: P09560
Secondary accession number(s): Q91390
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: October 29, 2014
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3