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Protein

RAF proto-oncogene serine/threonine-protein kinase

Gene

raf1

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Serine/threonine-protein kinase that acts as a regulatory link between the membrane-associated Ras GTPases and the MAPK/ERK cascade, and this critical regulatory link functions as a switch determining cell fate decisions. RAF1 activation initiates a mitogen-activated protein kinase (MAPK) cascade that comprises a sequential phosphorylation of the dual-specific MAPK kinases (MAP2K1/MEK1 and MAP2K2/MEK2) and the extracellular signal-regulated kinases (MAPK3/ERK1 and MAPK1/ERK2) (By similarity).By similarity

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi138Zinc 1By similarity1
Metal bindingi151Zinc 2By similarity1
Metal bindingi154Zinc 2By similarity1
Metal bindingi164Zinc 1By similarity1
Metal bindingi167Zinc 1By similarity1
Metal bindingi172Zinc 2By similarity1
Metal bindingi175Zinc 2By similarity1
Metal bindingi183Zinc 1By similarity1
Binding sitei366ATPPROSITE-ProRule annotation1
Active sitei459Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri137 – 183Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST47
Nucleotide bindingi346 – 354ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BRENDAi2.7.10.2. 6725.

Names & Taxonomyi

Protein namesi
Recommended name:
RAF proto-oncogene serine/threonine-protein kinase (EC:2.7.11.1)
Alternative name(s):
C-RAF
Gene namesi
Name:raf1
Synonyms:raf
OrganismiXenopus laevis (African clawed frog)
Taxonomic identifieri8355 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000866001 – 638RAF proto-oncogene serine/threonine-protein kinaseAdd BLAST638

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei43PhosphoserineBy similarity1
Modified residuei257PhosphoserineBy similarity1
Modified residuei266Phosphothreonine; by autocatalysisBy similarity1
Modified residuei329PhosphoserineBy similarity1
Modified residuei490PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation at Ser-257 inactivates kinase activity. Dephosphorylation of Ser-257 by a complex containing protein phosphatase 1 relieves inactivation, leading to stimulate RAF1 activity (By similarity).By similarity

Keywords - PTMi

Phosphoprotein

Interactioni

Protein-protein interaction databases

DIPiDIP-1075N.

Structurei

3D structure databases

ProteinModelPortaliP09560.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini56 – 130RBDPROSITE-ProRule annotationAdd BLAST75
Domaini340 – 600Protein kinasePROSITE-ProRule annotationAdd BLAST261

Sequence similaritiesi

Contains 1 phorbol-ester/DAG-type zinc finger.PROSITE-ProRule annotation
Contains 1 protein kinase domain.PROSITE-ProRule annotation
Contains 1 RBD (Ras-binding) domain.PROSITE-ProRule annotation

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri137 – 183Phorbol-ester/DAG-typePROSITE-ProRule annotationAdd BLAST47

Keywords - Domaini

Zinc-finger

Phylogenomic databases

HOVERGENiHBG001886.

Family and domain databases

CDDicd00029. C1. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR002219. PE/DAG-bd.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR003116. RBD_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00130. C1_1. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
PF02196. RBD. 1 hit.
[Graphical view]
SMARTiSM00109. C1. 1 hit.
SM00455. RBD. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50898. RBD. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P09560-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEHIQGAWKT LSNGFGFKES VFEGSSCMSP TIVHQFGYQR RASDDGKLSD
60 70 80 90 100
TSKTSSTMRV YLPNKQRTVV NVRSGMSLHD CLMKSLKVRG LQPECCAVFR
110 120 130 140 150
LIQDPKGKLR LDWNTDAMSL VGAELQVDFL DHVPLTTHNF VRKTFLKLAF
160 170 180 190 200
CDICQKFLLN AFRCQTCGYK FHEHCSTKVP TMCVDWSNIR QLLLFPNPNN
210 220 230 240 250
IEGGSHTLPS LTMRRIGESV RIPVSSQQRY STPHPFSFST PSPVSECSLS
260 270 280 290 300
QRQRSTSTPN VHMVSTTMAV DSRVIEDALR SHSESGSPNN LSPTGWSNAK
310 320 330 340 350
APAPTHREKA ASSTGQEKNK IRARGQRDSS YYWEIIASEV MLSSRIGSGS
360 370 380 390 400
FGTVYKGKWH GDVAVKILKV TDPTPEQLQA FRNEVAVLRK TRHVNILLFM
410 420 430 440 450
GYMTKDNLAI VTQWCEGSSL YYHLHVLDTK FQMFQLIDIA RQTAQGMDYL
460 470 480 490 500
HAKNIIHRDM KSNNIFLHEG LTVKIGDFGL ATVKTRWSGS QQVEQLTGSI
510 520 530 540 550
LWMAPEVIRM QDNNPFSFQS DVYSYGIVLY ELMTGELPYS HIRDRDQIIF
560 570 580 590 600
LVGRGGVVPD LSKLYKNCPK AMKRLVADSI KKLRDERPLF PQILSSIELL
610 620 630
QHSLPKINRS ALEPSLHRAA HTEDISSCAL TSTRLPVF
Length:638
Mass (Da):71,960
Last modified:July 1, 1989 - v1
Checksum:i1FF352BFFBF528DF
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti309K → R in AAB20707 (PubMed:1721855).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12948 mRNA. Translation: CAA31407.1.
S74063 mRNA. Translation: AAB20707.1.
PIRiS01930. TVXLRF.
UniGeneiXl.5697.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X12948 mRNA. Translation: CAA31407.1.
S74063 mRNA. Translation: AAB20707.1.
PIRiS01930. TVXLRF.
UniGeneiXl.5697.

3D structure databases

ProteinModelPortaliP09560.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-1075N.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG001886.

Enzyme and pathway databases

BRENDAi2.7.10.2. 6725.

Family and domain databases

CDDicd00029. C1. 1 hit.
InterProiIPR011009. Kinase-like_dom.
IPR002219. PE/DAG-bd.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR003116. RBD_dom.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR008271. Ser/Thr_kinase_AS.
IPR029071. Ubiquitin-rel_dom.
[Graphical view]
PfamiPF00130. C1_1. 1 hit.
PF07714. Pkinase_Tyr. 1 hit.
PF02196. RBD. 1 hit.
[Graphical view]
SMARTiSM00109. C1. 1 hit.
SM00455. RBD. 1 hit.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF54236. SSF54236. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
PS50898. RBD. 1 hit.
PS00479. ZF_DAG_PE_1. 1 hit.
PS50081. ZF_DAG_PE_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRAF1_XENLA
AccessioniPrimary (citable) accession number: P09560
Secondary accession number(s): Q91390
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: November 30, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.