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Protein

Phosphoribulokinase, chloroplastic

Gene
N/A
Organism
Spinacia oleracea (Spinach)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + D-ribulose 5-phosphate = ADP + D-ribulose 1,5-bisphosphate.

Enzyme regulationi

Light regulated via thioredoxin by reversible oxidation/reduction of sulfhydryl/disulfide groups.

Pathwayi

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. phosphoribulokinase activity Source: UniProtKB-EC

GO - Biological processi

  1. reductive pentose-phosphate cycle Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Calvin cycle, Photosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00116.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribulokinase, chloroplastic (EC:2.7.1.19)
Short name:
PRK
Short name:
PRKase
Alternative name(s):
Phosphopentokinase
OrganismiSpinacia oleracea (Spinach)
Taxonomic identifieri3562 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesAmaranthaceaeChenopodioideaeAnserineaeSpinacia

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5151Chloroplast2 PublicationsAdd
BLAST
Chaini52 – 402351Phosphoribulokinase, chloroplasticPRO_0000025754Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi67 ↔ 1061 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

PRIDEiP09559.

Structurei

3D structure databases

ProteinModelPortaliP09559.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the phosphoribulokinase family.Curated

Keywords - Domaini

Transit peptide

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR006082. PRK.
IPR006083. PRK/URK.
[Graphical view]
PfamiPF00485. PRK. 1 hit.
[Graphical view]
PRINTSiPR00478. PHRIBLKINASE.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00567. PHOSPHORIBULOKINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09559-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAVCTVYTIP TTTHLGSSFN QNNKQVFFNY KRSSSSNNTL FTTRPSYVIT
60 70 80 90 100
CSQQQTIVIG LAADSGCGKS TFMRRLTSVF GGAAEPPKGG NPDSNTLISD
110 120 130 140 150
TTTVICLDDF HSLDRNGRKV EKVTALDPKA NDFDLMYEQV KALKEGKAVD
160 170 180 190 200
KPIYNHVSGL LDPPELIQPP KILVIEGLHP MYDARVRELL DFSIYLDISN
210 220 230 240 250
EVKFAWKIQR DMKERGHSLE SIKASIESRK PDFDAYIDPQ KQHADVVIEV
260 270 280 290 300
LPTELIPDDD EGKVLRVRMI QKEGVKFFNP VYLFDEGSTI SWIPCGRKLT
310 320 330 340 350
CSYPGIKFSY GPDTFYGNEV TVVEMDGMFD RLDELIYVES HLSNLSTKFY
360 370 380 390 400
GEVTQQMLKH QNFPGSNNGT GFFQTIIGLK IRDLFEQLVA SRSTATATAA

KA
Length:402
Mass (Da):45,007
Last modified:July 1, 1989 - v1
Checksum:i450759B96A675C6B
GO

Sequence cautioni

The sequence AAA34036.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07654 mRNA. Translation: CAA30499.1.
M21338 mRNA. Translation: AAA34036.1. Different initiation.
PIRiS02099.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07654 mRNA. Translation: CAA30499.1.
M21338 mRNA. Translation: AAA34036.1. Different initiation.
PIRiS02099.

3D structure databases

ProteinModelPortaliP09559.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP09559.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayiUPA00116.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR006082. PRK.
IPR006083. PRK/URK.
[Graphical view]
PfamiPF00485. PRK. 1 hit.
[Graphical view]
PRINTSiPR00478. PHRIBLKINASE.
SUPFAMiSSF52540. SSF52540. 1 hit.
PROSITEiPS00567. PHOSPHORIBULOKINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Nucleotide sequence of spinach cDNA encoding phosphoribulokinase."
    Roesler K.R., Ogren W.L.
    Nucleic Acids Res. 16:7192-7192(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Cloning and sequencing of cDNA encoding the mature form of phosphoribulokinase from spinach."
    Milanez S., Mural R.J.
    Gene 66:55-63(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 7-402.
  3. "Purification and characterization of ribulose-5-phosphate kinase from spinach."
    Porter M.A., Milanez S., Stringer C.D., Hartman F.C.
    Arch. Biochem. Biophys. 245:14-23(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 52-69.
  4. "Commonality of catalytic and regulatory sites of spinach phosphoribulokinase: characterization of a tryptic peptide that contains an essential cysteinyl residue."
    Porter M.A., Hartman F.C.
    Biochemistry 25:7314-7318(1986)
    Cited for: PROTEIN SEQUENCE OF 52-69.
  5. "Characterization of the regulatory thioredoxin site of phosphoribulokinase."
    Porter M.A., Stringer C.D., Hartman F.C.
    J. Biol. Chem. 263:123-129(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISULFIDE BOND, PARTIAL PROTEIN SEQUENCE.

Entry informationi

Entry nameiKPPR_SPIOL
AccessioniPrimary (citable) accession number: P09559
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: January 7, 2015
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.