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P09559

- KPPR_SPIOL

UniProt

P09559 - KPPR_SPIOL

Protein

Phosphoribulokinase, chloroplastic

Gene
N/A
Organism
Spinacia oleracea (Spinach)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 77 (01 Oct 2014)
      Sequence version 1 (01 Jul 1989)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    ATP + D-ribulose 5-phosphate = ADP + D-ribulose 1,5-bisphosphate.

    Enzyme regulationi

    Light regulated via thioredoxin by reversible oxidation/reduction of sulfhydryl/disulfide groups.

    Pathwayi

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. phosphoribulokinase activity Source: UniProtKB-EC

    GO - Biological processi

    1. reductive pentose-phosphate cycle Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Calvin cycle, Photosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    UniPathwayiUPA00116.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoribulokinase, chloroplastic (EC:2.7.1.19)
    Short name:
    PRK
    Short name:
    PRKase
    Alternative name(s):
    Phosphopentokinase
    OrganismiSpinacia oleracea (Spinach)
    Taxonomic identifieri3562 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeCaryophyllalesAmaranthaceaeChenopodioideaeAnserineaeSpinacia

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 5151Chloroplast2 PublicationsAdd
    BLAST
    Chaini52 – 402351Phosphoribulokinase, chloroplasticPRO_0000025754Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi67 ↔ 1061 Publication

    Keywords - PTMi

    Disulfide bond

    Proteomic databases

    PRIDEiP09559.

    Structurei

    3D structure databases

    ProteinModelPortaliP09559.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the phosphoribulokinase family.Curated

    Keywords - Domaini

    Transit peptide

    Family and domain databases

    Gene3Di3.40.50.300. 1 hit.
    InterProiIPR027417. P-loop_NTPase.
    IPR006082. PRK.
    IPR006083. PRK/URK.
    [Graphical view]
    PfamiPF00485. PRK. 1 hit.
    [Graphical view]
    PRINTSiPR00478. PHRIBLKINASE.
    SUPFAMiSSF52540. SSF52540. 1 hit.
    PROSITEiPS00567. PHOSPHORIBULOKINASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P09559-1 [UniParc]FASTAAdd to Basket

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    MAVCTVYTIP TTTHLGSSFN QNNKQVFFNY KRSSSSNNTL FTTRPSYVIT    50
    CSQQQTIVIG LAADSGCGKS TFMRRLTSVF GGAAEPPKGG NPDSNTLISD 100
    TTTVICLDDF HSLDRNGRKV EKVTALDPKA NDFDLMYEQV KALKEGKAVD 150
    KPIYNHVSGL LDPPELIQPP KILVIEGLHP MYDARVRELL DFSIYLDISN 200
    EVKFAWKIQR DMKERGHSLE SIKASIESRK PDFDAYIDPQ KQHADVVIEV 250
    LPTELIPDDD EGKVLRVRMI QKEGVKFFNP VYLFDEGSTI SWIPCGRKLT 300
    CSYPGIKFSY GPDTFYGNEV TVVEMDGMFD RLDELIYVES HLSNLSTKFY 350
    GEVTQQMLKH QNFPGSNNGT GFFQTIIGLK IRDLFEQLVA SRSTATATAA 400
    KA 402
    Length:402
    Mass (Da):45,007
    Last modified:July 1, 1989 - v1
    Checksum:i450759B96A675C6B
    GO

    Sequence cautioni

    The sequence AAA34036.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X07654 mRNA. Translation: CAA30499.1.
    M21338 mRNA. Translation: AAA34036.1. Different initiation.
    PIRiS02099.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X07654 mRNA. Translation: CAA30499.1 .
    M21338 mRNA. Translation: AAA34036.1 . Different initiation.
    PIRi S02099.

    3D structure databases

    ProteinModelPortali P09559.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PRIDEi P09559.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00116 .

    Family and domain databases

    Gene3Di 3.40.50.300. 1 hit.
    InterProi IPR027417. P-loop_NTPase.
    IPR006082. PRK.
    IPR006083. PRK/URK.
    [Graphical view ]
    Pfami PF00485. PRK. 1 hit.
    [Graphical view ]
    PRINTSi PR00478. PHRIBLKINASE.
    SUPFAMi SSF52540. SSF52540. 1 hit.
    PROSITEi PS00567. PHOSPHORIBULOKINASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Nucleotide sequence of spinach cDNA encoding phosphoribulokinase."
      Roesler K.R., Ogren W.L.
      Nucleic Acids Res. 16:7192-7192(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Cloning and sequencing of cDNA encoding the mature form of phosphoribulokinase from spinach."
      Milanez S., Mural R.J.
      Gene 66:55-63(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 7-402.
    3. "Purification and characterization of ribulose-5-phosphate kinase from spinach."
      Porter M.A., Milanez S., Stringer C.D., Hartman F.C.
      Arch. Biochem. Biophys. 245:14-23(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 52-69.
    4. "Commonality of catalytic and regulatory sites of spinach phosphoribulokinase: characterization of a tryptic peptide that contains an essential cysteinyl residue."
      Porter M.A., Hartman F.C.
      Biochemistry 25:7314-7318(1986)
      Cited for: PROTEIN SEQUENCE OF 52-69.
    5. "Characterization of the regulatory thioredoxin site of phosphoribulokinase."
      Porter M.A., Stringer C.D., Hartman F.C.
      J. Biol. Chem. 263:123-129(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISULFIDE BOND, PARTIAL PROTEIN SEQUENCE.

    Entry informationi

    Entry nameiKPPR_SPIOL
    AccessioniPrimary (citable) accession number: P09559
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 77 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3