P09556 (UMPS_DICDI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Uridine 5'-monophosphate synthase
Short name=UMP synthase

Including the following 2 domains:

1. Orotate phosphoribosyltransferase
   Short name=OPRTase
   EC=2.4.2.10
2. Orotidine 5'-phosphate decarboxylase
   EC=4.1.1.23
   Alternative name(s):
   OMPdecase

Gene names

Name:	pyr56
Synonyms:	pyr5-6
ORF Names:	DDB_G0280041

Organism

Dictyostelium discoideum (Slime mold)

Taxonomic identifier

44689 [NCBI]

Taxonomic lineage

Eukaryota › Amoebozoa › Mycetozoa › Dictyosteliida › Dictyostelium

Protein attributes

Sequence length	478 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate. Orotidine 5'-phosphate = UMP + CO2.
Pathway	Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 1/2. Pyrimidine metabolism; UMP biosynthesis via de novo pathway; UMP from orotate: step 2/2.
Sequence similarities	In the N-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family. In the C-terminal section; belongs to the OMP decarboxylase family.

Ontologies

Keywords
Biological process	Pyrimidine biosynthesis
Molecular function	Decarboxylase Glycosyltransferase Lyase Transferase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological process	'de novo' UMP biosynthetic process Inferred from electronic annotation. Source: InterPro 'de novo' pyrimidine base biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	phagocytic vesicle Inferred from direct assay Ref.3. Source: dictyBase
Molecular function	orotate phosphoribosyltransferase activity Inferred from genetic interaction Ref.1. Source: dictyBase orotidine-5'-phosphate decarboxylase activity Inferred from mutant phenotype. Source: dictyBase
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 478	478	Uridine 5'-monophosphate synthase		PRO_0000139651
Regions
	Region	1 – 210	210	OPRTase
	Region	117 – 125	9	Phosphoribosyl pyrophosphate binding site Potential
	Region	211 – 229	19	Domain linker Potential
	Region	230 – 478	249	OMPdecase
Sites
	Active site	313	1	By similarity
Experimental info
	Sequence conflict	11 – 14	4	NEID → MKLM in CAA30443. Ref.1
	Sequence conflict	155	1	Q → E in CAA30443. Ref.1
	Sequence conflict	337	1	D → V in CAA30443. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P09556 [UniParc]. Last modified December 4, 2007. Version 2. Checksum: 0A5F45A4B0738636	FASTA	478	52,519
	10 20 30 40 50 60 MNIKELVLKL NEIDAIKLGE FKLKSGIISP IYIDLRVTVS SPPLLAAIAE MMYQKVYKSG 70 80 90 100 110 120 NAQETPALVC GVPYTALPIA TGMSIANNIP MVVRRKEAKA YGTKQLIEGR FKEGDNVLVV 130 140 150 160 170 180 EDLVTSGASV LETVRDLNSV GLKVTDVVVL LDRQQGARQA LEKQGYRLHS VFTMEELINT 190 200 210 220 230 240 LIEAGKLTGR TLELVQSFLD ANRNVVVPLP PTLAPPAPAP IVINKPFEER AKLASNPMAS 250 260 270 280 290 300 KLFTLMSSKK TNLAVAADLT DKQQLLDLAE SIGSEICVLK THVDIIDNYD EEFIKSLKCI 310 320 330 340 350 360 AAKHNFLIFE DRKFADIGNT VKYQFENGVY KISKWADMVT VHGVAGSSIV DGFKSGLKEY 370 380 390 400 410 420 GSGLLLLAQM SSKGSLCVGD YTTQMIEMAN NNKEEVMGLI CQERLPSMTD GLVLMTPGVQ 430 440 450 460 470 FNSTGDAMGQ QYNTPEYIIK EKNTDVIIVG RGIYQSNDPK SVANKYRTAA WETYQSKF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence analysis of the DdPYR5-6 gene coding for UMP synthase in Dictyostelium discoideum and comparison with orotate phosphoribosyl transferases and OMP decarboxylases." Jacquet M., Guilbaud R., Garreau H. Mol. Gen. Genet. 211:441-445(1988) [PubMed: 2835631] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: AX3.
[2]	"The genome of the social amoeba Dictyostelium discoideum." Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N. , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A. Nature 435:43-57(2005) [PubMed: 15875012] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: AX4.
[3]	"Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake." Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T. Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed: 16926386] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS]. Strain: AX2.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X07560 Genomic DNA. Translation: CAA30443.1. AAFI02000035 Genomic DNA. Translation: EAL67247.1.
PIR	S03826.
RefSeq	XP_641269.1. XM_636177.1.
3D structure databases
ProteinModelPortal	P09556.
ModBase	Search...
Protein-protein interaction databases
STRING	P09556.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblProtists	DDB0214958; DDB0214958; DDB_G0280041.
GeneID	8622401.
GenomeReviews	Gene locus pyr56 in contig CM000152_GR.
KEGG	ddi:DDB_G0280041.
Organism-specific databases
dictyBase	DDB_G0280041. pyr56.
Phylogenomic databases
eggNOG	KOG1377.
HOGENOM	HBG326699.
OMA	AFKFGDF.
PhylomeDB	P09556.
ProtClustDB	CLSZ2430632.
Family and domain databases
InterPro	IPR013785. Aldolase_TIM. IPR014732. OMPdecase. IPR018089. OMPdecase_AS. IPR001754. OMPdeCOase_dom. IPR004467. Or_phspho_trans_clade-1. IPR023031. Orotate_PribosylTferase. IPR000836. PRibTrfase. IPR011060. RibuloseP-bd_barrel. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
KO	K13421.
Pfam	PF00215. OMPdecase. 1 hit. PF00156. Pribosyltran. 1 hit. [Graphical view]
SMART	SM00934. OMPdecase. 1 hit. [Graphical view]
SUPFAM	SSF51366. RibP_bind_barrel. 1 hit.
TIGRFAMs	TIGR00336. PyrE. 1 hit. TIGR01740. PyrF. 1 hit.
PROSITE	PS00156. OMPDECASE. 1 hit. PS00103. PUR_PYR_PR_TRANSFER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UMPS_DICDI

Accession

Primary (citable) accession number: P09556
Secondary accession number(s): Q54VT6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	December 4, 2007
Last modified:	November 16, 2011
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)