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P09556

- UMPS_DICDI

UniProt

P09556 - UMPS_DICDI

Protein

Uridine 5'-monophosphate synthase

Gene

pyr56

Organism
Dictyostelium discoideum (Slime mold)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 2 (04 Dec 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    Orotidine 5'-phosphate + diphosphate = orotate + 5-phospho-alpha-D-ribose 1-diphosphate.
    Orotidine 5'-phosphate = UMP + CO2.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei313 – 3131By similarity

    GO - Molecular functioni

    1. orotate phosphoribosyltransferase activity Source: dictyBase
    2. orotidine-5'-phosphate decarboxylase activity Source: dictyBase

    GO - Biological processi

    1. 'de novo' pyrimidine nucleobase biosynthetic process Source: InterPro
    2. 'de novo' UMP biosynthetic process Source: UniProtKB-UniPathway
    3. pyrimidine nucleobase biosynthetic process Source: dictyBase

    Keywords - Molecular functioni

    Decarboxylase, Glycosyltransferase, Lyase, Transferase

    Keywords - Biological processi

    Pyrimidine biosynthesis

    Enzyme and pathway databases

    UniPathwayiUPA00070; UER00119.
    UPA00070; UER00120.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Uridine 5'-monophosphate synthase
    Short name:
    UMP synthase
    Including the following 2 domains:
    Orotate phosphoribosyltransferase (EC:2.4.2.10)
    Short name:
    OPRTase
    Orotidine 5'-phosphate decarboxylase (EC:4.1.1.23)
    Alternative name(s):
    OMPdecase
    Gene namesi
    Name:pyr56
    Synonyms:pyr5-6
    ORF Names:DDB_G0280041
    OrganismiDictyostelium discoideum (Slime mold)
    Taxonomic identifieri44689 [NCBI]
    Taxonomic lineageiEukaryotaAmoebozoaMycetozoaDictyosteliidaDictyostelium
    ProteomesiUP000002195: Chromosome 3, UP000002195: Unassembled WGS sequence

    Organism-specific databases

    dictyBaseiDDB_G0280041. pyr56.

    Subcellular locationi

    GO - Cellular componenti

    1. phagocytic vesicle Source: dictyBase

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 478478Uridine 5'-monophosphate synthasePRO_0000139651Add
    BLAST

    Proteomic databases

    PRIDEiP09556.

    Interactioni

    Protein-protein interaction databases

    STRINGi44689.DDB_0214958.

    Structurei

    3D structure databases

    ProteinModelPortaliP09556.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni1 – 210210OPRTaseAdd
    BLAST
    Regioni117 – 1259Phosphoribosyl pyrophosphate binding siteSequence Analysis
    Regioni211 – 22919Domain linkerSequence AnalysisAdd
    BLAST
    Regioni230 – 478249OMPdecaseAdd
    BLAST

    Sequence similaritiesi

    In the N-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.Curated
    In the C-terminal section; belongs to the OMP decarboxylase family.Curated

    Phylogenomic databases

    eggNOGiCOG0284.
    KOiK13421.
    OMAiSMKPEFL.
    PhylomeDBiP09556.

    Family and domain databases

    Gene3Di3.20.20.70. 1 hit.
    3.40.50.2020. 1 hit.
    HAMAPiMF_01208. PyrE.
    InterProiIPR013785. Aldolase_TIM.
    IPR014732. OMPdecase.
    IPR018089. OMPdecase_AS.
    IPR001754. OMPdeCOase_dom.
    IPR023031. OPRT.
    IPR004467. Or_phspho_trans_dom.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    IPR011060. RibuloseP-bd_barrel.
    [Graphical view]
    PfamiPF00215. OMPdecase. 1 hit.
    PF00156. Pribosyltran. 1 hit.
    [Graphical view]
    SMARTiSM00934. OMPdecase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51366. SSF51366. 1 hit.
    SSF53271. SSF53271. 1 hit.
    TIGRFAMsiTIGR00336. pyrE. 1 hit.
    TIGR01740. pyrF. 1 hit.
    PROSITEiPS00156. OMPDECASE. 1 hit.
    PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P09556-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNIKELVLKL NEIDAIKLGE FKLKSGIISP IYIDLRVTVS SPPLLAAIAE    50
    MMYQKVYKSG NAQETPALVC GVPYTALPIA TGMSIANNIP MVVRRKEAKA 100
    YGTKQLIEGR FKEGDNVLVV EDLVTSGASV LETVRDLNSV GLKVTDVVVL 150
    LDRQQGARQA LEKQGYRLHS VFTMEELINT LIEAGKLTGR TLELVQSFLD 200
    ANRNVVVPLP PTLAPPAPAP IVINKPFEER AKLASNPMAS KLFTLMSSKK 250
    TNLAVAADLT DKQQLLDLAE SIGSEICVLK THVDIIDNYD EEFIKSLKCI 300
    AAKHNFLIFE DRKFADIGNT VKYQFENGVY KISKWADMVT VHGVAGSSIV 350
    DGFKSGLKEY GSGLLLLAQM SSKGSLCVGD YTTQMIEMAN NNKEEVMGLI 400
    CQERLPSMTD GLVLMTPGVQ FNSTGDAMGQ QYNTPEYIIK EKNTDVIIVG 450
    RGIYQSNDPK SVANKYRTAA WETYQSKF 478
    Length:478
    Mass (Da):52,519
    Last modified:December 4, 2007 - v2
    Checksum:i0A5F45A4B0738636
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti11 – 144NEID → MKLM in CAA30443. (PubMed:2835631)Curated
    Sequence conflicti155 – 1551Q → E in CAA30443. (PubMed:2835631)Curated
    Sequence conflicti337 – 3371D → V in CAA30443. (PubMed:2835631)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X07560 Genomic DNA. Translation: CAA30443.1.
    AAFI02000035 Genomic DNA. Translation: EAL67247.1.
    PIRiS03826.
    RefSeqiXP_641269.1. XM_636177.1.

    Genome annotation databases

    EnsemblProtistsiDDB0214958; DDB0214958; DDB_G0280041.
    GeneIDi8622401.
    KEGGiddi:DDB_G0280041.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X07560 Genomic DNA. Translation: CAA30443.1 .
    AAFI02000035 Genomic DNA. Translation: EAL67247.1 .
    PIRi S03826.
    RefSeqi XP_641269.1. XM_636177.1.

    3D structure databases

    ProteinModelPortali P09556.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 44689.DDB_0214958.

    Proteomic databases

    PRIDEi P09556.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblProtistsi DDB0214958 ; DDB0214958 ; DDB_G0280041 .
    GeneIDi 8622401.
    KEGGi ddi:DDB_G0280041.

    Organism-specific databases

    dictyBasei DDB_G0280041. pyr56.

    Phylogenomic databases

    eggNOGi COG0284.
    KOi K13421.
    OMAi SMKPEFL.
    PhylomeDBi P09556.

    Enzyme and pathway databases

    UniPathwayi UPA00070 ; UER00119 .
    UPA00070 ; UER00120 .

    Miscellaneous databases

    PROi P09556.

    Family and domain databases

    Gene3Di 3.20.20.70. 1 hit.
    3.40.50.2020. 1 hit.
    HAMAPi MF_01208. PyrE.
    InterProi IPR013785. Aldolase_TIM.
    IPR014732. OMPdecase.
    IPR018089. OMPdecase_AS.
    IPR001754. OMPdeCOase_dom.
    IPR023031. OPRT.
    IPR004467. Or_phspho_trans_dom.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    IPR011060. RibuloseP-bd_barrel.
    [Graphical view ]
    Pfami PF00215. OMPdecase. 1 hit.
    PF00156. Pribosyltran. 1 hit.
    [Graphical view ]
    SMARTi SM00934. OMPdecase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51366. SSF51366. 1 hit.
    SSF53271. SSF53271. 1 hit.
    TIGRFAMsi TIGR00336. pyrE. 1 hit.
    TIGR01740. pyrF. 1 hit.
    PROSITEi PS00156. OMPDECASE. 1 hit.
    PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence analysis of the DdPYR5-6 gene coding for UMP synthase in Dictyostelium discoideum and comparison with orotate phosphoribosyl transferases and OMP decarboxylases."
      Jacquet M., Guilbaud R., Garreau H.
      Mol. Gen. Genet. 211:441-445(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: AX3.
    2. "The genome of the social amoeba Dictyostelium discoideum."
      Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R., Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B., Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T., Lehmann R., Hamlin N.
      , Davies R., Gaudet P., Fey P., Pilcher K., Chen G., Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N., Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E., Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N., Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D., Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T., Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D., Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A., Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M., Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A., Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y., Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C., Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R., Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.
      Nature 435:43-57(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: AX4.
    3. "Proteomics fingerprinting of phagosome maturation and evidence for the role of a Galpha during uptake."
      Gotthardt D., Blancheteau V., Bosserhoff A., Ruppert T., Delorenzi M., Soldati T.
      Mol. Cell. Proteomics 5:2228-2243(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: AX2.

    Entry informationi

    Entry nameiUMPS_DICDI
    AccessioniPrimary (citable) accession number: P09556
    Secondary accession number(s): Q54VT6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: December 4, 2007
    Last modified: October 1, 2014
    This is version 104 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Multifunctional enzyme, Reference proteome

    Documents

    1. Dictyostelium discoideum
      Dictyostelium discoideum: entries, gene names and cross-references to dictyBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3