P09544 (WNT2_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein Wnt-2

Alternative name(s):
Int-1-like protein 1
Int-1-related protein
Short name=IRP

Gene names

Name:	WNT2
Synonyms:	INT1L1, IRP

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	360 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Ligand for members of the frizzled family of seven transmembrane receptors. Probable developmental protein. May be a signaling molecule which affects the development of discrete regions of tissues. Is likely to signal over only few cell diameters.
Subcellular location	Secreted › extracellular space › extracellular matrix.
Tissue specificity	Expressed in brain in the thalamus, in fetal and adult lung and in placenta. Ref.6
Post-translational modification	Palmitoylation at Ser-212 is required for efficient binding to frizzled receptors. It is also required for subsequent palmitoylation at Cys-76. Palmitoylation is necessary for proper trafficking to cell surface By similarity.
Sequence similarities	Belongs to the Wnt family.

Ontologies

Keywords
Biological process	Wnt signaling pathway
Cellular component	Extracellular matrix Secreted
Coding sequence diversity	Polymorphism
Domain	Signal
Molecular function	Developmental protein
PTM	Glycoprotein Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	atrial cardiac muscle tissue morphogenesis Inferred from Biological aspect of Ancestor. Source: RefGenome canonical Wnt receptor signaling pathway Inferred from direct assay PubMed 10347172. Source: BHF-UCL cardiac epithelial to mesenchymal transition Inferred from Biological aspect of Ancestor. Source: RefGenome cellular response to retinoic acid Inferred from sequence or structural similarity. Source: UniProtKB cellular response to transforming growth factor beta stimulus Inferred from expression pattern PubMed 15040835. Source: UniProtKB dorsal/ventral axis specification Inferred from Biological aspect of Ancestor. Source: RefGenome iris morphogenesis Inferred from sequence or structural similarity PubMed 16258938. Source: BHF-UCL labyrinthine layer blood vessel development Inferred from Biological aspect of Ancestor. Source: RefGenome lens development in camera-type eye Inferred from sequence or structural similarity PubMed 16258938. Source: BHF-UCL lung induction Inferred from Biological aspect of Ancestor. Source: RefGenome mammary gland epithelium development Inferred from expression pattern PubMed 8168088. Source: UniProtKB neuron differentiation Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of canonical Wnt receptor signaling pathway Inferred from mutant phenotype PubMed 17948129. Source: UniProtKB positive regulation of cardiac muscle cell proliferation Inferred from Biological aspect of Ancestor. Source: RefGenome positive regulation of epithelial cell proliferation involved in lung morphogenesis Inferred from Biological aspect of Ancestor. Source: RefGenome positive regulation of fibroblast proliferation Inferred from mutant phenotype PubMed 19734317. Source: BHF-UCL positive regulation of mesenchymal cell proliferation Inferred from Biological aspect of Ancestor. Source: RefGenome positive regulation of sequence-specific DNA binding transcription factor activity Inferred from direct assay PubMed 10557084. Source: BHF-UCL positive regulation of transcription from RNA polymerase II promoter Inferred from Biological aspect of Ancestor. Source: RefGenome
Cellular_component	cytoplasm Inferred from direct assay PubMed 19038973PubMed 19239325. Source: UniProtKB extracellular matrix Inferred from direct assay PubMed 15507471. Source: UniProtKB extracellular space Inferred from Biological aspect of Ancestor. Source: RefGenome proteinaceous extracellular matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	cytokine activity Inferred from direct assay PubMed 10557084. Source: BHF-UCL frizzled-2 binding Inferred from Biological aspect of Ancestor. Source: RefGenome receptor agonist activity Inferred by curator PubMed 17948129. Source: BHF-UCL
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 360	335	Protein Wnt-2		PRO_0000041410
Amino acid modifications
	Lipidation	76	1	S-palmitoyl cysteine By similarity
	Lipidation	212	1	O-palmitoyl serine; by PORCN By similarity
	Glycosylation	295	1	N-linked (GlcNAc...) Potential
Natural variations
	Natural variant	5	1	L → R. Ref.6		VAR_013865
	Natural variant	294	1	C → F. Corresponds to variant rs1051751 [ dbSNP | Ensembl ].		VAR_052954
	Natural variant	299	1	R → W. Ref.6		VAR_013866

Sequences

	Sequence		Length	Mass (Da)
	P09544 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: AA96325BF68C4788	FASTA	360	40,418
	10 20 30 40 50 60 MNAPLGGIWL WLPLLLTWLT PEVNSSWWYM RATGGSSRVM CDNVPGLVSS QRQLCHRHPD 70 80 90 100 110 120 VMRAISQGVA EWTAECQHQF RQHRWNCNTL DRDHSLFGRV LLRSSRESAF VYAISSAGVV 130 140 150 160 170 180 FAITRACSQG EVKSCSCDPK KMGSAKDSKG IFDWGGCSDN IDYGIKFARA FVDAKERKGK 190 200 210 220 230 240 DARALMNLHN NRAGRKAVKR FLKQECKCHG VSGSCTLRTC WLAMADFRKT GDYLWRKYNG 250 260 270 280 290 300 AIQVVMNQDG TGFTVANERF KKPTKNDLVY FENSPDYCIR DREAGSLGTA GRVCNLTSRG 310 320 330 340 350 360 MDSCEVMCCG RGYDTSHVTR MTKCGCKFHW CCAVRCQDCL EALDVHTCKA PKNADWTTAT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Isolation of a human gene with protein sequence similarity to human and murine int-1 and the Drosophila segment polarity mutant wingless." Wainwright B.J., Scambler P.J., Stanier P., Watson E.K., Bell G., Wicking C., Estivill X., Courtney M., Boue A., Pedersen P.S., Williamson R., Farrall M. EMBO J. 7:1743-1748(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Lung.
[2]	Farrall M. Submitted (APR-1988) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (OCT-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
[4]	"The DNA sequence of human chromosome 7." Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L. , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K. Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Brain, Lung and Testis.
[6]	"Evidence supporting WNT2 as an autism susceptibility gene." Wassink T.H., Piven J., Vieland V.J., Huang J., Swiderski R.E., Pietila J., Braun T., Beck G., Folstein S.E., Haines J.L., Sheffield V.C. Am. J. Med. Genet. 105:406-413(2001) [PubMed] [Europe PMC] [Abstract] Cited for: VARIANTS ARG-5 AND TRP-299, TISSUE SPECIFICITY.
[7]	"No association between the WNT2 gene and autistic disorder." McCoy P.A., Shao Y., Wolpert C.M., Donnelly S.L., Ashley-Koch A., Abel H.L., Ravan S.A., Abramson R.K., Wright H.H., DeLong G.R., Cuccaro M.L., Gilbert J.R., Pericak-Vance M.A. Am. J. Med. Genet. 114:106-109(2002) [PubMed] [Europe PMC] [Abstract] Cited for: SHOWS THAT WNT2 IS NOT LINKED TO AUTISM SUSCEPTIBILITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X07876 mRNA. Translation: CAA30725.1. BT019608 mRNA. Translation: AAV38415.1. AC002465 Genomic DNA. Translation: AAB67043.1. AC006326 Genomic DNA. Translation: AAD28351.1. BC029854 mRNA. Translation: AAH29854.1. BC078170 mRNA. Translation: AAH78170.1.
IPI	IPI00015175.
PIR	S00834.
RefSeq	NP_003382.1. NM_003391.2.
UniGene	Hs.567356.
3D structure databases
ProteinModelPortal	P09544.
ModBase	Search...
Protein-protein interaction databases
IntAct	P09544. 4 interactions.
STRING	9606.ENSP00000265441.
PTM databases
PhosphoSite	P09544.
Polymorphism databases
DMDM	139750.
Proteomic databases
PaxDb	P09544.
PRIDE	P09544.
Protocols and materials databases
DNASU	7472.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000265441; ENSP00000265441; ENSG00000105989.
GeneID	7472.
KEGG	hsa:7472.
UCSC	uc003viz.3. human.
Organism-specific databases
CTD	7472.
GeneCards	GC07M116916.
HGNC	HGNC:12780. WNT2.
HPA	CAB017098. HPA020920.
MIM	147870. gene.
neXtProt	NX_P09544.
PharmGKB	PA37381.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG284879.
HOGENOM	HOG000039529.
HOVERGEN	HBG001595.
InParanoid	P09544.
KO	K00182.
OMA	DVMRAIG.
OrthoDB	EOG4255SV.
PhylomeDB	P09544.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction.
Gene expression databases
ArrayExpress	P09544.
Bgee	P09544.
CleanEx	HS_WNT2.
Genevestigator	P09544.
GermOnline	ENSG00000105989. Homo sapiens.
Family and domain databases
InterPro	IPR005817. Wnt. IPR009140. Wnt2. IPR018161. Wnt_CS. [Graphical view]
PANTHER	PTHR12027. PTHR12027. 1 hit. PTHR12027:SF21. PTHR12027:SF21. 1 hit.
Pfam	PF00110. wnt. 1 hit. [Graphical view]
PRINTS	PR01842. WNT2PROTEIN. PR01349. WNTPROTEIN.
SMART	SM00097. WNT1. 1 hit. [Graphical view]
PROSITE	PS00246. WNT1. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P09544.
ChEMBL	CHEMBL1255132.
GenomeRNAi	7472.
NextBio	29268.
SOURCE	Search...

Entry information

Entry name

WNT2_HUMAN

Accession

Primary (citable) accession number: P09544
Secondary accession number(s): Q75N05, Q9UDP9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	April 3, 2013
This is version 128 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.