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Protein

2',3'-cyclic-nucleotide 3'-phosphodiesterase

Gene

CNP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May participate in RNA metabolism in the myelinating cell, CNP is the third most abundant protein in central nervous system myelin.By similarity

Catalytic activityi

Nucleoside 2',3'-cyclic phosphate + H2O = nucleoside 2'-phosphate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei251 – 2511Proton acceptor
Binding sitei253 – 2531Substrate
Active sitei330 – 3301Proton donor
Binding sitei332 – 3321Substrate

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BRENDAi3.1.4.37. 2681.

Names & Taxonomyi

Protein namesi
Recommended name:
2',3'-cyclic-nucleotide 3'-phosphodiesterase (EC:3.1.4.37)
Short name:
CNP
Short name:
CNPase
Gene namesi
Name:CNP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:2158. CNP.

Subcellular locationi

  • Membrane; Lipid-anchor
  • Melanosome

  • Note: Firmly bound to membrane structures of brain white matter. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

GO - Cellular componenti

  • cytoplasm Source: BHF-UCL
  • extracellular exosome Source: UniProtKB
  • extracellular space Source: BHF-UCL
  • melanosome Source: UniProtKB-SubCell
  • membrane Source: UniProtKB
  • microtubule Source: UniProtKB
  • microvillus Source: Ensembl
  • mitochondrial inner membrane Source: Ensembl
  • mitochondrial outer membrane Source: Ensembl
  • myelin sheath abaxonal region Source: Ensembl
  • myelin sheath adaxonal region Source: Ensembl
  • nucleoplasm Source: HPA
  • nucleus Source: HPA
  • perinuclear region of cytoplasm Source: Ensembl
  • plasma membrane Source: Ensembl
  • pseudopodium Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA26680.

Polymorphism and mutation databases

BioMutaiCNP.
DMDMi1705945.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 4184182',3'-cyclic-nucleotide 3'-phosphodiesterasePRO_0000089961Add
BLAST
Propeptidei419 – 4213Removed in mature formBy similarityPRO_0000422296

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei110 – 1101PhosphotyrosineBy similarity
Modified residuei418 – 4181Cysteine methyl esterBy similarity
Lipidationi418 – 4181S-farnesyl cysteineBy similarity

Keywords - PTMi

Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

MaxQBiP09543.
PaxDbiP09543.
PRIDEiP09543.

2D gel databases

UCD-2DPAGEP09543.

PTM databases

PhosphoSiteiP09543.

Expressioni

Gene expression databases

BgeeiP09543.
CleanExiHS_CNP.
ExpressionAtlasiP09543. baseline and differential.
GenevestigatoriP09543.

Organism-specific databases

HPAiCAB002672.
HPA023266.
HPA023278.
HPA023280.
HPA023338.

Interactioni

Subunit structurei

Exists as monomers and homodimers.By similarity

Protein-protein interaction databases

BioGridi107667. 15 interactions.
IntActiP09543. 8 interactions.
MINTiMINT-4999529.
STRINGi9606.ENSP00000377470.

Structurei

Secondary structure

1
421
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi188 – 1947Combined sources
Helixi196 – 21520Combined sources
Helixi217 – 2215Combined sources
Helixi223 – 2264Combined sources
Helixi238 – 2414Combined sources
Beta strandi251 – 2566Combined sources
Helixi258 – 2603Combined sources
Helixi265 – 2706Combined sources
Helixi272 – 2776Combined sources
Beta strandi281 – 29111Combined sources
Beta strandi293 – 3019Combined sources
Helixi306 – 3094Combined sources
Turni325 – 3284Combined sources
Beta strandi330 – 3356Combined sources
Helixi343 – 35614Combined sources
Beta strandi362 – 3676Combined sources
Beta strandi370 – 3767Combined sources
Beta strandi379 – 39719Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WOJX-ray1.80A186-399[»]
ProteinModelPortaliP09543.
SMRiP09543. Positions 47-75, 186-399.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09543.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiNOG314041.
GeneTreeiENSGT00510000048410.
HOGENOMiHOG000111838.
HOVERGENiHBG001451.
KOiK01121.
OMAiLWPNDVD.
PhylomeDBiP09543.
TreeFamiTF332157.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR008431. CNPase.
IPR027417. P-loop_NTPase.
IPR009097. RNA_ligase/cNuc_Pdiesterase.
[Graphical view]
PANTHERiPTHR10156. PTHR10156. 1 hit.
PfamiPF05881. CNPase. 1 hit.
[Graphical view]
PIRSFiPIRSF000970. CNPase. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF55144. SSF55144. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform CNPII (identifier: P09543-1) [UniParc]FASTAAdd to basket

Also known as: DNAII

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNRGFSRKSH TFLPKIFFRK MSSSGAKDKP ELQFPFLQDE DTVATLLECK
60 70 80 90 100
TLFILRGLPG SGKSTLARVI VDKYRDGTKM VSADAYKITP GARGAFSEEY
110 120 130 140 150
KRLDEDLAAY CRRRDIRILV LDDTNHERER LEQLFEMADQ YQYQVVLVEP
160 170 180 190 200
KTAWRLDCAQ LKEKNQWQLS ADDLKKLKPG LEKDFLPLYF GWFLTKKSSE
210 220 230 240 250
TLRKAGQVFL EELGNHKAFK KELRQFVPGD EPREKMDLVT YFGKRPPGVL
260 270 280 290 300
HCTTKFCDYG KAPGAEEYAQ QDVLKKSYSK AFTLTISALF VTPKTTGARV
310 320 330 340 350
ELSEQQLQLW PSDVDKLSPT DNLPRGSRAH ITLGCAADVE AVQTGLDLLE
360 370 380 390 400
ILRQEKGGSR GEEVGELSRG KLYSLGNGRW MLTLAKNMEV RAIFTGYYGK
410 420
GKPVPTQGSR KGGALQSCTI I
Length:421
Mass (Da):47,579
Last modified:October 1, 1996 - v2
Checksum:iCA6D0097DFD87255
GO
Isoform CNPI (identifier: P09543-2) [UniParc]FASTAAdd to basket

Also known as: DNAI

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: Missing.

Show »
Length:401
Mass (Da):45,099
Checksum:i81F0E080822D3B0D
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti207 – 2071Q → R.
Corresponds to variant rs34353668 [ dbSNP | Ensembl ].
VAR_033746

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 2020Missing in isoform CNPI. 2 PublicationsVSP_004171Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S46849
, S46843, S46845, S46846 Genomic DNA. Translation: AAB23928.2.
M19650 mRNA. Translation: AAA35704.1.
D13146 Genomic DNA. Translation: BAA39694.1.
D13146 Genomic DNA. Translation: BAA02435.1.
S50017
, S50013, S50014, S50016 Genomic DNA. Translation: AAB24298.2.
AC125257 Genomic DNA. No translation available.
BC001362 mRNA. Translation: AAH01362.1.
BC006392 mRNA. Translation: AAH06392.1.
BC011046 mRNA. Translation: AAH11046.1.
BC028040 mRNA. Translation: AAH28040.1.
CCDSiCCDS11414.2. [P09543-1]
PIRiJC1517.
RefSeqiNP_149124.3. NM_033133.4. [P09543-1]
XP_006721764.1. XM_006721701.2. [P09543-2]
UniGeneiHs.273621.

Genome annotation databases

EnsembliENST00000393888; ENSP00000377466; ENSG00000173786. [P09543-2]
ENST00000393892; ENSP00000377470; ENSG00000173786. [P09543-1]
GeneIDi1267.
KEGGihsa:1267.
UCSCiuc002hyl.1. human. [P09543-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S46849
, S46843, S46845, S46846 Genomic DNA. Translation: AAB23928.2.
M19650 mRNA. Translation: AAA35704.1.
D13146 Genomic DNA. Translation: BAA39694.1.
D13146 Genomic DNA. Translation: BAA02435.1.
S50017
, S50013, S50014, S50016 Genomic DNA. Translation: AAB24298.2.
AC125257 Genomic DNA. No translation available.
BC001362 mRNA. Translation: AAH01362.1.
BC006392 mRNA. Translation: AAH06392.1.
BC011046 mRNA. Translation: AAH11046.1.
BC028040 mRNA. Translation: AAH28040.1.
CCDSiCCDS11414.2. [P09543-1]
PIRiJC1517.
RefSeqiNP_149124.3. NM_033133.4. [P09543-1]
XP_006721764.1. XM_006721701.2. [P09543-2]
UniGeneiHs.273621.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1WOJX-ray1.80A186-399[»]
ProteinModelPortaliP09543.
SMRiP09543. Positions 47-75, 186-399.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107667. 15 interactions.
IntActiP09543. 8 interactions.
MINTiMINT-4999529.
STRINGi9606.ENSP00000377470.

PTM databases

PhosphoSiteiP09543.

Polymorphism and mutation databases

BioMutaiCNP.
DMDMi1705945.

2D gel databases

UCD-2DPAGEP09543.

Proteomic databases

MaxQBiP09543.
PaxDbiP09543.
PRIDEiP09543.

Protocols and materials databases

DNASUi1267.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000393888; ENSP00000377466; ENSG00000173786. [P09543-2]
ENST00000393892; ENSP00000377470; ENSG00000173786. [P09543-1]
GeneIDi1267.
KEGGihsa:1267.
UCSCiuc002hyl.1. human. [P09543-1]

Organism-specific databases

CTDi1267.
GeneCardsiGC17P040118.
HGNCiHGNC:2158. CNP.
HPAiCAB002672.
HPA023266.
HPA023278.
HPA023280.
HPA023338.
MIMi123830. gene.
neXtProtiNX_P09543.
PharmGKBiPA26680.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG314041.
GeneTreeiENSGT00510000048410.
HOGENOMiHOG000111838.
HOVERGENiHBG001451.
KOiK01121.
OMAiLWPNDVD.
PhylomeDBiP09543.
TreeFamiTF332157.

Enzyme and pathway databases

BRENDAi3.1.4.37. 2681.

Miscellaneous databases

ChiTaRSiCNP. human.
EvolutionaryTraceiP09543.
GeneWikii2%27,3%27-Cyclic-nucleotide_3%27-phosphodiesterase.
GenomeRNAii1267.
NextBioi5129.
PROiP09543.
SOURCEiSearch...

Gene expression databases

BgeeiP09543.
CleanExiHS_CNP.
ExpressionAtlasiP09543. baseline and differential.
GenevestigatoriP09543.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR008431. CNPase.
IPR027417. P-loop_NTPase.
IPR009097. RNA_ligase/cNuc_Pdiesterase.
[Graphical view]
PANTHERiPTHR10156. PTHR10156. 1 hit.
PfamiPF05881. CNPase. 1 hit.
[Graphical view]
PIRSFiPIRSF000970. CNPase. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF55144. SSF55144. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "2',3'-cyclic nucleotide-3'-phosphohydrolase and signal transduction in central nervous system myelin."
    Thompson R.J.
    Biochem. Soc. Trans. 20:621-626(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "cDNA cloning and amino acid sequence of human brain 2',3'-cyclic-nucleotide 3'-phosphodiesterase."
    Kurihara T., Takahashi Y., Nishiyama A., Kumanishi T.
    Biochem. Biophys. Res. Commun. 152:837-842(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM CNPI).
    Tissue: Brain.
  3. "Structure, expression and chromosomal localization of the gene encoding human 2',3'-cyclic-nucleotide 3'-phosphodiesterase."
    Monoh K., Kurihara T., Takahashi Y., Ichikawa T., Kumanishi T., Hayashi S., Minoshima S., Shimizu N.
    Gene 129:297-301(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "Structure and chromosomal localization of the human 2',3'-cyclic nucleotide 3'-phosphodiesterase gene."
    Douglas A.J., Fox M.F., Abbott C.M., Hinks L.J., Sharpe G., Povey S., Thompson R.J.
    Ann. Hum. Genet. 56:243-254(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  5. "DNA sequence of human chromosome 17 and analysis of rearrangement in the human lineage."
    Zody M.C., Garber M., Adams D.J., Sharpe T., Harrow J., Lupski J.R., Nicholson C., Searle S.M., Wilming L., Young S.K., Abouelleil A., Allen N.R., Bi W., Bloom T., Borowsky M.L., Bugalter B.E., Butler J., Chang J.L.
    , Chen C.-K., Cook A., Corum B., Cuomo C.A., de Jong P.J., DeCaprio D., Dewar K., FitzGerald M., Gilbert J., Gibson R., Gnerre S., Goldstein S., Grafham D.V., Grocock R., Hafez N., Hagopian D.S., Hart E., Norman C.H., Humphray S., Jaffe D.B., Jones M., Kamal M., Khodiyar V.K., LaButti K., Laird G., Lehoczky J., Liu X., Lokyitsang T., Loveland J., Lui A., Macdonald P., Major J.E., Matthews L., Mauceli E., McCarroll S.A., Mihalev A.H., Mudge J., Nguyen C., Nicol R., O'Leary S.B., Osoegawa K., Schwartz D.C., Shaw-Smith C., Stankiewicz P., Steward C., Swarbreck D., Venkataraman V., Whittaker C.A., Yang X., Zimmer A.R., Bradley A., Hubbard T., Birren B.W., Rogers J., Lander E.S., Nusbaum C.
    Nature 440:1045-1049(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  6. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS CNPI AND CNPII).
    Tissue: Brain and Skin.
  7. "The epitope recognized by a monoclonal antibody in the myelin-associated protein CNP."
    Stricker R., Kalbacher H., Reiser G.
    Biochem. Biophys. Res. Commun. 237:266-270(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 21-58.
  8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Tissue: Melanoma.
  9. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    Tissue: Melanoma.
  10. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "An enzyme assisted RP-RPLC approach for in-depth analysis of human liver phosphoproteome."
    Bian Y., Song C., Cheng K., Dong M., Wang F., Huang J., Sun D., Wang L., Ye M., Zou H.
    J. Proteomics 96:253-262(2014) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  13. "Crystal structure of the catalytic fragment of human brain 2',3'-cyclic-nucleotide 3'-phosphodiesterase."
    Sakamoto Y., Tanaka N., Ichimiya T., Kurihara T., Nakamura K.T.
    J. Mol. Biol. 346:789-800(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS) OF 186-399 IN COMPLEX WITH PHOSPHATE.

Entry informationi

Entry nameiCN37_HUMAN
AccessioniPrimary (citable) accession number: P09543
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: October 1, 1996
Last modified: May 27, 2015
This is version 163 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.