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Protein

2',3'-cyclic-nucleotide 3'-phosphodiesterase

Gene

CNP

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

May participate in RNA metabolism in the myelinating cell, CNP is the third most abundant protein in central nervous system myelin.By similarity

Catalytic activityi

Nucleoside 2',3'-cyclic phosphate + H2O = nucleoside 2'-phosphate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei251Proton acceptor1
Binding sitei253Substrate1
Active sitei330Proton donor1
Binding sitei332Substrate1

GO - Molecular functioni

  • 2',3'-cyclic-nucleotide 3'-phosphodiesterase activity Source: ProtInc
  • cyclic nucleotide binding Source: Ensembl
  • RNA binding Source: UniProtKB-KW

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciZFISH:HS10729-MONOMER.
BRENDAi3.1.4.37. 2681.

Names & Taxonomyi

Protein namesi
Recommended name:
2',3'-cyclic-nucleotide 3'-phosphodiesterase (EC:3.1.4.37)
Short name:
CNP
Short name:
CNPase
Gene namesi
Name:CNP
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 17

Organism-specific databases

HGNCiHGNC:2158. CNP.

Subcellular locationi

  • Membrane; Lipid-anchor
  • Melanosome

  • Note: Firmly bound to membrane structures of brain white matter. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.

GO - Cellular componenti

  • cytoplasm Source: BHF-UCL
  • extracellular exosome Source: UniProtKB
  • extracellular space Source: BHF-UCL
  • melanosome Source: UniProtKB-SubCell
  • membrane Source: UniProtKB
  • microtubule Source: UniProtKB
  • microvillus Source: Ensembl
  • mitochondrial inner membrane Source: Ensembl
  • mitochondrial outer membrane Source: Ensembl
  • myelin sheath abaxonal region Source: Ensembl
  • myelin sheath adaxonal region Source: Ensembl
  • nucleoplasm Source: HPA
  • nucleus Source: HPA
  • perinuclear region of cytoplasm Source: Ensembl
  • plasma membrane Source: Ensembl
  • pseudopodium Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi1267.
OpenTargetsiENSG00000173786.
PharmGKBiPA26680.

Polymorphism and mutation databases

BioMutaiCNP.
DMDMi1705945.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000899611 – 4182',3'-cyclic-nucleotide 3'-phosphodiesteraseAdd BLAST418
PropeptideiPRO_0000422296419 – 421Removed in mature formBy similarity3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei6PhosphoserineCombined sources1
Modified residuei9PhosphoserineCombined sources1
Modified residuei110PhosphotyrosineBy similarity1
Modified residuei170PhosphoserineCombined sources1
Modified residuei359PhosphoserineBy similarity1
Modified residuei418Cysteine methyl esterBy similarity1
Lipidationi418S-farnesyl cysteineBy similarity1

Keywords - PTMi

Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

EPDiP09543.
MaxQBiP09543.
PaxDbiP09543.
PeptideAtlasiP09543.
PRIDEiP09543.

2D gel databases

UCD-2DPAGEP09543.

PTM databases

iPTMnetiP09543.
PhosphoSitePlusiP09543.
SwissPalmiP09543.

Expressioni

Gene expression databases

BgeeiENSG00000173786.
CleanExiHS_CNP.
ExpressionAtlasiP09543. baseline and differential.
GenevisibleiP09543. HS.

Organism-specific databases

HPAiCAB002672.
HPA023266.
HPA023278.
HPA023280.
HPA023338.

Interactioni

Subunit structurei

Exists as monomers and homodimers.By similarity

Protein-protein interaction databases

BioGridi107667. 18 interactors.
IntActiP09543. 33 interactors.
MINTiMINT-4999529.
STRINGi9606.ENSP00000377470.

Structurei

Secondary structure

1421
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi188 – 194Combined sources7
Helixi196 – 215Combined sources20
Helixi217 – 221Combined sources5
Helixi223 – 226Combined sources4
Helixi238 – 241Combined sources4
Beta strandi251 – 256Combined sources6
Helixi258 – 260Combined sources3
Helixi265 – 270Combined sources6
Helixi272 – 277Combined sources6
Beta strandi281 – 291Combined sources11
Beta strandi293 – 301Combined sources9
Helixi306 – 309Combined sources4
Turni325 – 328Combined sources4
Beta strandi330 – 335Combined sources6
Helixi343 – 356Combined sources14
Beta strandi362 – 367Combined sources6
Beta strandi370 – 376Combined sources7
Beta strandi379 – 397Combined sources19

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WOJX-ray1.80A186-399[»]
ProteinModelPortaliP09543.
SMRiP09543.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09543.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG410IT06. Eukaryota.
ENOG4111F3B. LUCA.
GeneTreeiENSGT00510000048410.
HOGENOMiHOG000111838.
HOVERGENiHBG001451.
KOiK01121.
OMAiLWPNDVD.
OrthoDBiEOG091G0I8Z.
PhylomeDBiP09543.
TreeFamiTF332157.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR008431. CNPase.
IPR027417. P-loop_NTPase.
IPR009097. RNA_ligase/cNuc_Pdiesterase.
[Graphical view]
PANTHERiPTHR10156. PTHR10156. 1 hit.
PfamiPF05881. CNPase. 1 hit.
[Graphical view]
PIRSFiPIRSF000970. CNPase. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF55144. SSF55144. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform CNPII (identifier: P09543-1) [UniParc]FASTAAdd to basket
Also known as: DNAII

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MNRGFSRKSH TFLPKIFFRK MSSSGAKDKP ELQFPFLQDE DTVATLLECK
60 70 80 90 100
TLFILRGLPG SGKSTLARVI VDKYRDGTKM VSADAYKITP GARGAFSEEY
110 120 130 140 150
KRLDEDLAAY CRRRDIRILV LDDTNHERER LEQLFEMADQ YQYQVVLVEP
160 170 180 190 200
KTAWRLDCAQ LKEKNQWQLS ADDLKKLKPG LEKDFLPLYF GWFLTKKSSE
210 220 230 240 250
TLRKAGQVFL EELGNHKAFK KELRQFVPGD EPREKMDLVT YFGKRPPGVL
260 270 280 290 300
HCTTKFCDYG KAPGAEEYAQ QDVLKKSYSK AFTLTISALF VTPKTTGARV
310 320 330 340 350
ELSEQQLQLW PSDVDKLSPT DNLPRGSRAH ITLGCAADVE AVQTGLDLLE
360 370 380 390 400
ILRQEKGGSR GEEVGELSRG KLYSLGNGRW MLTLAKNMEV RAIFTGYYGK
410 420
GKPVPTQGSR KGGALQSCTI I
Length:421
Mass (Da):47,579
Last modified:October 1, 1996 - v2
Checksum:iCA6D0097DFD87255
GO
Isoform CNPI (identifier: P09543-2) [UniParc]FASTAAdd to basket
Also known as: DNAI

The sequence of this isoform differs from the canonical sequence as follows:
     1-20: Missing.

Show »
Length:401
Mass (Da):45,099
Checksum:i81F0E080822D3B0D
GO

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_033746207Q → R.Corresponds to variant rs34353668dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0041711 – 20Missing in isoform CNPI. 2 PublicationsAdd BLAST20

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S46849
, S46843, S46845, S46846 Genomic DNA. Translation: AAB23928.2.
M19650 mRNA. Translation: AAA35704.1.
D13146 Genomic DNA. Translation: BAA39694.1.
D13146 Genomic DNA. Translation: BAA02435.1.
S50017
, S50013, S50014, S50016 Genomic DNA. Translation: AAB24298.2.
AC125257 Genomic DNA. No translation available.
BC001362 mRNA. Translation: AAH01362.1.
BC006392 mRNA. Translation: AAH06392.1.
BC011046 mRNA. Translation: AAH11046.1.
BC028040 mRNA. Translation: AAH28040.1.
CCDSiCCDS11414.2. [P09543-1]
CCDS82123.1. [P09543-2]
PIRiJC1517.
RefSeqiNP_001317145.1. NM_001330216.1.
NP_149124.3. NM_033133.4. [P09543-1]
XP_011522642.1. XM_011524340.2. [P09543-2]
UniGeneiHs.273621.

Genome annotation databases

EnsembliENST00000393888; ENSP00000377466; ENSG00000173786. [P09543-2]
ENST00000393892; ENSP00000377470; ENSG00000173786. [P09543-1]
GeneIDi1267.
KEGGihsa:1267.
UCSCiuc002hyl.2. human. [P09543-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S46849
, S46843, S46845, S46846 Genomic DNA. Translation: AAB23928.2.
M19650 mRNA. Translation: AAA35704.1.
D13146 Genomic DNA. Translation: BAA39694.1.
D13146 Genomic DNA. Translation: BAA02435.1.
S50017
, S50013, S50014, S50016 Genomic DNA. Translation: AAB24298.2.
AC125257 Genomic DNA. No translation available.
BC001362 mRNA. Translation: AAH01362.1.
BC006392 mRNA. Translation: AAH06392.1.
BC011046 mRNA. Translation: AAH11046.1.
BC028040 mRNA. Translation: AAH28040.1.
CCDSiCCDS11414.2. [P09543-1]
CCDS82123.1. [P09543-2]
PIRiJC1517.
RefSeqiNP_001317145.1. NM_001330216.1.
NP_149124.3. NM_033133.4. [P09543-1]
XP_011522642.1. XM_011524340.2. [P09543-2]
UniGeneiHs.273621.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WOJX-ray1.80A186-399[»]
ProteinModelPortaliP09543.
SMRiP09543.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107667. 18 interactors.
IntActiP09543. 33 interactors.
MINTiMINT-4999529.
STRINGi9606.ENSP00000377470.

PTM databases

iPTMnetiP09543.
PhosphoSitePlusiP09543.
SwissPalmiP09543.

Polymorphism and mutation databases

BioMutaiCNP.
DMDMi1705945.

2D gel databases

UCD-2DPAGEP09543.

Proteomic databases

EPDiP09543.
MaxQBiP09543.
PaxDbiP09543.
PeptideAtlasiP09543.
PRIDEiP09543.

Protocols and materials databases

DNASUi1267.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000393888; ENSP00000377466; ENSG00000173786. [P09543-2]
ENST00000393892; ENSP00000377470; ENSG00000173786. [P09543-1]
GeneIDi1267.
KEGGihsa:1267.
UCSCiuc002hyl.2. human. [P09543-1]

Organism-specific databases

CTDi1267.
DisGeNETi1267.
GeneCardsiCNP.
HGNCiHGNC:2158. CNP.
HPAiCAB002672.
HPA023266.
HPA023278.
HPA023280.
HPA023338.
MIMi123830. gene.
neXtProtiNX_P09543.
OpenTargetsiENSG00000173786.
PharmGKBiPA26680.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiENOG410IT06. Eukaryota.
ENOG4111F3B. LUCA.
GeneTreeiENSGT00510000048410.
HOGENOMiHOG000111838.
HOVERGENiHBG001451.
KOiK01121.
OMAiLWPNDVD.
OrthoDBiEOG091G0I8Z.
PhylomeDBiP09543.
TreeFamiTF332157.

Enzyme and pathway databases

BioCyciZFISH:HS10729-MONOMER.
BRENDAi3.1.4.37. 2681.

Miscellaneous databases

ChiTaRSiCNP. human.
EvolutionaryTraceiP09543.
GeneWikii2%27,3%27-Cyclic-nucleotide_3%27-phosphodiesterase.
GenomeRNAii1267.
PROiP09543.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000173786.
CleanExiHS_CNP.
ExpressionAtlasiP09543. baseline and differential.
GenevisibleiP09543. HS.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR008431. CNPase.
IPR027417. P-loop_NTPase.
IPR009097. RNA_ligase/cNuc_Pdiesterase.
[Graphical view]
PANTHERiPTHR10156. PTHR10156. 1 hit.
PfamiPF05881. CNPase. 1 hit.
[Graphical view]
PIRSFiPIRSF000970. CNPase. 1 hit.
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF55144. SSF55144. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiCN37_HUMAN
AccessioniPrimary (citable) accession number: P09543
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 180 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 17
    Human chromosome 17: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.