P09535 (IGF2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Insulin-like growth factor II

Alternative name(s):
IGF-II
Multiplication-stimulating polypeptide

Cleaved into the following 2 chains:

1. Insulin-like growth factor II
2. Preptin

Gene names

Name:	Igf2
Synonyms:	Igf-2

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	180 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development. Ref.7 Ref.8 Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3. Ref.7 Ref.8
Subcellular location	Secreted.
Developmental stage	Low levels of expression during myoblast proliferation. Levels rise rapidly during myoblast differentiation and then decrease.
Sequence similarities	Belongs to the insulin family.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glucose metabolism Osteogenesis
Cellular component	Secreted
Domain	Signal
Molecular function	Growth factor Hormone Mitogen
PTM	Cleavage on pair of basic residues Disulfide bond
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	exocrine pancreas development Inferred from mutant phenotype PubMed 12101187. Source: MGI glucose metabolic process Inferred from electronic annotation. Source: UniProtKB-KW insulin receptor signaling pathway via phosphatidylinositol 3-kinase cascade Inferred from mutant phenotype PubMed 20032056. Source: BHF-UCL organ morphogenesis Inferred from mutant phenotype PubMed 12101187. Source: MGI osteoblast differentiation Inferred from expression pattern PubMed 16311053. Source: BHF-UCL positive regulation of cell division Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of glycogen (starch) synthase activity Inferred from mutant phenotype PubMed 20032056. Source: BHF-UCL positive regulation of glycogen biosynthetic process Inferred from mutant phenotype PubMed 20032056. Source: BHF-UCL positive regulation of insulin receptor signaling pathway Inferred from mutant phenotype PubMed 20032056. Source: BHF-UCL positive regulation of peptidyl-tyrosine phosphorylation Inferred from mutant phenotype PubMed 20032056. Source: BHF-UCL positive regulation of transcription from RNA polymerase II promoter Inferred from genetic interaction PubMed 18762576. Source: MGI striated muscle cell differentiation Inferred from genetic interaction PubMed 18762576. Source: MGI
Cellular_component	extracellular space Inferred from direct assay PubMed 18762576. Source: MGI
Molecular_function	protein serine/threonine kinase activator activity Inferred from mutant phenotype PubMed 20032056. Source: BHF-UCL receptor activator activity Inferred from mutant phenotype PubMed 20032056. Source: BHF-UCL
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 24	24
	Chain	25 – 91	67	Insulin-like growth factor II		PRO_0000015720
	Propeptide	92 – 180	89	E peptide		PRO_0000015721
	Peptide	93 – 126	34	Preptin Ref.7		PRO_0000370377
Regions
	Region	25 – 52	28	B
	Region	53 – 64	12	C
	Region	65 – 85	21	A
	Region	86 – 91	6	D
Amino acid modifications
	Disulfide bond	33 ↔ 71		By similarity
	Disulfide bond	45 ↔ 84		By similarity
	Disulfide bond	70 ↔ 75		By similarity

Sequences

	Sequence		Length	Mass (Da)
	P09535 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 01730F8856EE6D7B	FASTA	180	20,030
	10 20 30 40 50 60 MGIPVGKSML VLLISLAFAL CCIAAYGPGE TLCGGELVDT LQFVCSDRGF YFSRPSSRAN 70 80 90 100 110 120 RRSRGIVEEC CFRSCDLALL ETYCATPAKS ERDVSTSQAV LPDDFPRYPV GKFFQYDTWR 130 140 150 160 170 180 QSAGRLRRGL PALLRARRGR MLAKELKEFR EAKRHRPLIV LPPKDPAHGG ASSEMSSNHQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of a placental cDNA encoding the mouse insulin-like growth factor II precursor." Stempien M.M., Fong N.M., Rall L.B., Bell G.I. DNA 5:357-361(1986) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Evolution of insulin-like growth factor II: characterization of the mouse IGF-II gene and identification of two pseudo-exons." Rotwein P., Hall L.J. DNA Cell Biol. 9:725-735(1990) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Nucleotide sequence of a 28-kb mouse genomic region comprising the imprinted Igf2 gene." Sasaki H., Shimozaki K., Zubair M., Aoki N., Hatano N., Moore T., Feil R., Constancia M., Reik W., Rotwein P. DNA Res. 3:331-335(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Embryo.
[5]	"Coordinate expression of insulin-like growth factor II and its receptor during muscle differentiation." Tollefsen S.E., Sadow J.L., Rotwein P. Proc. Natl. Acad. Sci. U.S.A. 86:1543-1547(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-52.
[6]	"Differential expression of the human, mouse and rat IGF-II genes." Holthuizen P.E., Cleutjens C.B., Veenstra G.J., van der Lee F.M., Koonen-Reemst A.M., Sussenbach J.S. Regul. Pept. 48:77-89(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-52 AND 103-180. Strain: BALB/c. Tissue: Spleen.
[7]	"Preptin derived from proinsulin-like growth factor II (proIGF-II) is secreted from pancreatic islet beta-cells and enhances insulin secretion." Buchanan C.M., Phillips A.R., Cooper G.J. Biochem. J. 360:431-439(2001) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 93-126, INSULIN-ENHANCING FUNCTION OF PREPTIN.
[8]	"Preptin, another peptide product of the pancreatic beta-cell, is osteogenic in vitro and in vivo." Cornish J., Callon K.E., Bava U., Watson M., Xu X., Lin J.M., Chan V.A., Grey A.B., Naot D., Buchanan C.M., Cooper G.J., Reid I.R. Am. J. Physiol. 292:E117-E122(2007) [PubMed] [Europe PMC] [Abstract] Cited for: OSTEOGENIC FUNCTION OF PREPTIN.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M14951 mRNA. Translation: AAA37683.1. M36332, M36331 Genomic DNA. Translation: AAA37926.1. U71085 Genomic DNA. Translation: AAC53516.1. BC053489 mRNA. Translation: AAH53489.1. M24633 Genomic DNA. Translation: AAA37923.1. X71921 Genomic DNA. Translation: CAA50737.1. X71922 Genomic DNA. Translation: CAA50738.1.
IPI	IPI00138397.
PIR	A24913.
RefSeq	NP_001116208.1. NM_001122736.1. NP_001116209.1. NM_001122737.1.
UniGene	Mm.3862.
3D structure databases
ProteinModelPortal	P09535.
SMR	P09535. Positions 30-88.
ModBase	Search...
PTM databases
PhosphoSite	P09535.
Proteomic databases
PaxDb	P09535.
PRIDE	P09535.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000000033; ENSMUSP00000000033; ENSMUSG00000048583. ENSMUST00000097936; ENSMUSP00000095549; ENSMUSG00000048583. ENSMUST00000105935; ENSMUSP00000101555; ENSMUSG00000048583. ENSMUST00000105936; ENSMUSP00000101556; ENSMUSG00000048583.
GeneID	16002.
KEGG	mmu:16002.
UCSC	uc009kod.2. mouse.
Organism-specific databases
CTD	3481.
MGI	MGI:96434. Igf2.
Phylogenomic databases
eggNOG	NOG44017.
GeneTree	ENSGT00530000063856.
HOGENOM	HOG000233362.
HOVERGEN	HBG006137.
InParanoid	P09535.
KO	K13769.
OrthoDB	EOG4HMJBH.
Gene expression databases
ArrayExpress	P09535.
Bgee	P09535.
CleanEx	MM_IGF2.
Genevestigator	P09535.
GermOnline	ENSMUSG00000048583. Mus musculus.
Family and domain databases
Gene3D	1.10.100.10. 1 hit.
InterPro	IPR022334. IGF2. IPR013576. IGF2_C. IPR016179. Insulin-like. IPR022350. Insulin-like_growth_factor. IPR022353. Insulin_CS. IPR022352. Insulin_family. [Graphical view]
Pfam	PF08365. IGF2_C. 1 hit. PF00049. Insulin. 1 hit. [Graphical view]
PRINTS	PR02002. INSLNLIKEGF. PR02006. INSLNLIKEGF2. PR00276. INSULINFAMLY.
ProDom	PD005188. IGF2_C. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00078. IlGF. 1 hit. [Graphical view]
SUPFAM	SSF56994. Insulin-like. 1 hit.
PROSITE	PS00262. INSULIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
ChiTaRS	IGF2. mouse.
NextBio	288794.
SOURCE	Search...

Entry information

Entry name

IGF2_MOUSE

Accession

Primary (citable) accession number: P09535

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	April 3, 2013
This is version 118 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program