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Protein

Insulin-like growth factor II

Gene

Igf2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.
Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.

GO - Molecular functioni

  • growth factor activity Source: MGI
  • insulin-like growth factor receptor binding Source: MGI
  • insulin receptor binding Source: MGI
  • protein serine/threonine kinase activator activity Source: BHF-UCL
  • receptor activator activity Source: BHF-UCL

GO - Biological processi

  • exocrine pancreas development Source: MGI
  • glucose metabolic process Source: UniProtKB-KW
  • insulin receptor signaling pathway via phosphatidylinositol 3-kinase Source: BHF-UCL
  • organ morphogenesis Source: MGI
  • osteoblast differentiation Source: BHF-UCL
  • positive regulation of activated T cell proliferation Source: MGI
  • positive regulation of catalytic activity Source: BHF-UCL
  • positive regulation of glycogen (starch) synthase activity Source: BHF-UCL
  • positive regulation of glycogen biosynthetic process Source: BHF-UCL
  • positive regulation of insulin receptor signaling pathway Source: BHF-UCL
  • positive regulation of MAPK cascade Source: MGI
  • positive regulation of mitotic nuclear division Source: MGI
  • positive regulation of peptidyl-tyrosine phosphorylation Source: BHF-UCL
  • positive regulation of protein kinase B signaling Source: MGI
  • positive regulation of protein phosphorylation Source: BHF-UCL
  • positive regulation of protein serine/threonine kinase activity Source: GOC
  • positive regulation of receptor activity Source: GOC
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • striated muscle cell differentiation Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Growth factor, Hormone, Mitogen

Keywords - Biological processi

Carbohydrate metabolism, Glucose metabolism, Osteogenesis

Enzyme and pathway databases

ReactomeiREACT_282332. Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R).
REACT_307071. Platelet degranulation.
REACT_321558. SHC-related events triggered by IGF1R.
REACT_331346. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).
REACT_346521. IRS-related events triggered by IGF1R.

Names & Taxonomyi

Protein namesi
Recommended name:
Insulin-like growth factor II
Alternative name(s):
IGF-II
Multiplication-stimulating polypeptide
Cleaved into the following 2 chains:
Gene namesi
Name:Igf2
Synonyms:Igf-2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:96434. Igf2.

Subcellular locationi

GO - Cellular componenti

  • extracellular exosome Source: MGI
  • extracellular space Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2424Add
BLAST
Chaini25 – 9167Insulin-like growth factor IIPRO_0000015720Add
BLAST
Propeptidei92 – 18089E peptidePRO_0000015721Add
BLAST
Peptidei93 – 12634PreptinPRO_0000370377Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi33 ↔ 71By similarity
Disulfide bondi45 ↔ 84By similarity
Disulfide bondi70 ↔ 75By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond

Proteomic databases

MaxQBiP09535.
PaxDbiP09535.
PRIDEiP09535.

PTM databases

PhosphoSiteiP09535.

Expressioni

Developmental stagei

Low levels of expression during myoblast proliferation. Levels rise rapidly during myoblast differentiation and then decrease.

Gene expression databases

BgeeiP09535.
CleanExiMM_IGF2.
ExpressionAtlasiP09535. baseline and differential.
GenevisibleiP09535. MM.

Interactioni

Protein-protein interaction databases

BioGridi200549. 2 interactions.
STRINGi10090.ENSMUSP00000114076.

Structurei

3D structure databases

ProteinModelPortaliP09535.
SMRiP09535. Positions 30-88.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni25 – 5228BAdd
BLAST
Regioni53 – 6412CAdd
BLAST
Regioni65 – 8521AAdd
BLAST
Regioni86 – 916D

Sequence similaritiesi

Belongs to the insulin family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG44017.
GeneTreeiENSGT00530000063856.
HOGENOMiHOG000233362.
HOVERGENiHBG006137.
InParanoidiP09535.
KOiK13769.
OrthoDBiEOG7TF7CG.
PhylomeDBiP09535.
TreeFamiTF332820.

Family and domain databases

Gene3Di1.10.100.10. 1 hit.
InterProiIPR022334. IGF2.
IPR013576. IGF2_C.
IPR016179. Insulin-like.
IPR022350. Insulin-like_growth_factor.
IPR022353. Insulin_CS.
IPR022352. Insulin_family.
[Graphical view]
PfamiPF08365. IGF2_C. 1 hit.
PF00049. Insulin. 1 hit.
[Graphical view]
PRINTSiPR02002. INSLNLIKEGF.
PR02006. INSLNLIKEGF2.
PR00276. INSULINFAMLY.
ProDomiPD005188. IGF2_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00078. IlGF. 1 hit.
[Graphical view]
SUPFAMiSSF56994. SSF56994. 1 hit.
PROSITEiPS00262. INSULIN. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09535-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGIPVGKSML VLLISLAFAL CCIAAYGPGE TLCGGELVDT LQFVCSDRGF
60 70 80 90 100
YFSRPSSRAN RRSRGIVEEC CFRSCDLALL ETYCATPAKS ERDVSTSQAV
110 120 130 140 150
LPDDFPRYPV GKFFQYDTWR QSAGRLRRGL PALLRARRGR MLAKELKEFR
160 170 180
EAKRHRPLIV LPPKDPAHGG ASSEMSSNHQ
Length:180
Mass (Da):20,030
Last modified:July 1, 1989 - v1
Checksum:i01730F8856EE6D7B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14951 mRNA. Translation: AAA37683.1.
M36332, M36331 Genomic DNA. Translation: AAA37926.1.
U71085 Genomic DNA. Translation: AAC53516.1.
BC053489 mRNA. Translation: AAH53489.1.
M24633 Genomic DNA. Translation: AAA37923.1.
X71921 Genomic DNA. Translation: CAA50737.1.
X71922 Genomic DNA. Translation: CAA50738.1.
CCDSiCCDS52458.1.
PIRiA24913.
RefSeqiNP_001116208.1. NM_001122736.1.
NP_001116209.1. NM_001122737.1.
XP_006508550.1. XM_006508487.1.
UniGeneiMm.3862.

Genome annotation databases

EnsembliENSMUST00000000033; ENSMUSP00000000033; ENSMUSG00000048583.
ENSMUST00000097936; ENSMUSP00000095549; ENSMUSG00000048583.
ENSMUST00000105935; ENSMUSP00000101555; ENSMUSG00000048583.
ENSMUST00000105936; ENSMUSP00000101556; ENSMUSG00000048583.
GeneIDi16002.
KEGGimmu:16002.
UCSCiuc009kod.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M14951 mRNA. Translation: AAA37683.1.
M36332, M36331 Genomic DNA. Translation: AAA37926.1.
U71085 Genomic DNA. Translation: AAC53516.1.
BC053489 mRNA. Translation: AAH53489.1.
M24633 Genomic DNA. Translation: AAA37923.1.
X71921 Genomic DNA. Translation: CAA50737.1.
X71922 Genomic DNA. Translation: CAA50738.1.
CCDSiCCDS52458.1.
PIRiA24913.
RefSeqiNP_001116208.1. NM_001122736.1.
NP_001116209.1. NM_001122737.1.
XP_006508550.1. XM_006508487.1.
UniGeneiMm.3862.

3D structure databases

ProteinModelPortaliP09535.
SMRiP09535. Positions 30-88.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200549. 2 interactions.
STRINGi10090.ENSMUSP00000114076.

PTM databases

PhosphoSiteiP09535.

Proteomic databases

MaxQBiP09535.
PaxDbiP09535.
PRIDEiP09535.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000000033; ENSMUSP00000000033; ENSMUSG00000048583.
ENSMUST00000097936; ENSMUSP00000095549; ENSMUSG00000048583.
ENSMUST00000105935; ENSMUSP00000101555; ENSMUSG00000048583.
ENSMUST00000105936; ENSMUSP00000101556; ENSMUSG00000048583.
GeneIDi16002.
KEGGimmu:16002.
UCSCiuc009kod.2. mouse.

Organism-specific databases

CTDi3481.
MGIiMGI:96434. Igf2.

Phylogenomic databases

eggNOGiNOG44017.
GeneTreeiENSGT00530000063856.
HOGENOMiHOG000233362.
HOVERGENiHBG006137.
InParanoidiP09535.
KOiK13769.
OrthoDBiEOG7TF7CG.
PhylomeDBiP09535.
TreeFamiTF332820.

Enzyme and pathway databases

ReactomeiREACT_282332. Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R).
REACT_307071. Platelet degranulation.
REACT_321558. SHC-related events triggered by IGF1R.
REACT_331346. Regulation of Insulin-like Growth Factor (IGF) transport and uptake by Insulin-like Growth Factor Binding Proteins (IGFBPs).
REACT_346521. IRS-related events triggered by IGF1R.

Miscellaneous databases

ChiTaRSiIgf2. mouse.
NextBioi288794.
PROiP09535.
SOURCEiSearch...

Gene expression databases

BgeeiP09535.
CleanExiMM_IGF2.
ExpressionAtlasiP09535. baseline and differential.
GenevisibleiP09535. MM.

Family and domain databases

Gene3Di1.10.100.10. 1 hit.
InterProiIPR022334. IGF2.
IPR013576. IGF2_C.
IPR016179. Insulin-like.
IPR022350. Insulin-like_growth_factor.
IPR022353. Insulin_CS.
IPR022352. Insulin_family.
[Graphical view]
PfamiPF08365. IGF2_C. 1 hit.
PF00049. Insulin. 1 hit.
[Graphical view]
PRINTSiPR02002. INSLNLIKEGF.
PR02006. INSLNLIKEGF2.
PR00276. INSULINFAMLY.
ProDomiPD005188. IGF2_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTiSM00078. IlGF. 1 hit.
[Graphical view]
SUPFAMiSSF56994. SSF56994. 1 hit.
PROSITEiPS00262. INSULIN. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Sequence of a placental cDNA encoding the mouse insulin-like growth factor II precursor."
    Stempien M.M., Fong N.M., Rall L.B., Bell G.I.
    DNA 5:357-361(1986) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "Evolution of insulin-like growth factor II: characterization of the mouse IGF-II gene and identification of two pseudo-exons."
    Rotwein P., Hall L.J.
    DNA Cell Biol. 9:725-735(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  3. "Nucleotide sequence of a 28-kb mouse genomic region comprising the imprinted Igf2 gene."
    Sasaki H., Shimozaki K., Zubair M., Aoki N., Hatano N., Moore T., Feil R., Constancia M., Reik W., Rotwein P.
    DNA Res. 3:331-335(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryo.
  5. "Coordinate expression of insulin-like growth factor II and its receptor during muscle differentiation."
    Tollefsen S.E., Sadow J.L., Rotwein P.
    Proc. Natl. Acad. Sci. U.S.A. 86:1543-1547(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-52.
  6. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-52 AND 103-180.
    Strain: BALB/c.
    Tissue: Spleen.
  7. "Preptin derived from proinsulin-like growth factor II (proIGF-II) is secreted from pancreatic islet beta-cells and enhances insulin secretion."
    Buchanan C.M., Phillips A.R., Cooper G.J.
    Biochem. J. 360:431-439(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 93-126, INSULIN-ENHANCING FUNCTION OF PREPTIN.
  8. "Preptin, another peptide product of the pancreatic beta-cell, is osteogenic in vitro and in vivo."
    Cornish J., Callon K.E., Bava U., Watson M., Xu X., Lin J.M., Chan V.A., Grey A.B., Naot D., Buchanan C.M., Cooper G.J., Reid I.R.
    Am. J. Physiol. 292:E117-E122(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: OSTEOGENIC FUNCTION OF PREPTIN.

Entry informationi

Entry nameiIGF2_MOUSE
AccessioniPrimary (citable) accession number: P09535
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: June 24, 2015
This is version 138 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.