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P09535 (IGF2_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 128. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Insulin-like growth factor II
Alternative name(s):
IGF-II
Multiplication-stimulating polypeptide

Cleaved into the following 2 chains:

  1. Insulin-like growth factor II
  2. Preptin
Gene names
Name:Igf2
Synonyms:Igf-2
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length180 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development. Ref.7 Ref.8

Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3. Ref.7 Ref.8

Subcellular location

Secreted.

Developmental stage

Low levels of expression during myoblast proliferation. Levels rise rapidly during myoblast differentiation and then decrease.

Sequence similarities

Belongs to the insulin family.

Ontologies

Keywords
   Biological processCarbohydrate metabolism
Glucose metabolism
Osteogenesis
   Cellular componentSecreted
   DomainSignal
   Molecular functionGrowth factor
Hormone
Mitogen
   PTMCleavage on pair of basic residues
Disulfide bond
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processexocrine pancreas development

Inferred from mutant phenotype PubMed 12101187. Source: MGI

glucose metabolic process

Inferred from electronic annotation. Source: UniProtKB-KW

insulin receptor signaling pathway via phosphatidylinositol 3-kinase

Inferred from mutant phenotype PubMed 20032056. Source: BHF-UCL

organ morphogenesis

Inferred from mutant phenotype PubMed 12101187. Source: MGI

osteoblast differentiation

Inferred from expression pattern PubMed 16311053. Source: BHF-UCL

positive regulation of catalytic activity

Inferred from mutant phenotype PubMed 20032056. Source: BHF-UCL

positive regulation of cell division

Inferred from electronic annotation. Source: UniProtKB-KW

positive regulation of glycogen (starch) synthase activity

Inferred from mutant phenotype PubMed 20032056. Source: BHF-UCL

positive regulation of glycogen biosynthetic process

Inferred from mutant phenotype PubMed 20032056. Source: BHF-UCL

positive regulation of insulin receptor signaling pathway

Inferred from mutant phenotype PubMed 20032056. Source: BHF-UCL

positive regulation of peptidyl-tyrosine phosphorylation

Inferred from mutant phenotype PubMed 20032056. Source: BHF-UCL

positive regulation of protein phosphorylation

Inferred from mutant phenotype PubMed 20032056. Source: BHF-UCL

positive regulation of protein serine/threonine kinase activity

Inferred from mutant phenotype PubMed 20032056. Source: GOC

positive regulation of receptor activity

Inferred from mutant phenotype PubMed 20032056. Source: GOC

positive regulation of transcription from RNA polymerase II promoter

Inferred from genetic interaction PubMed 18762576. Source: MGI

striated muscle cell differentiation

Inferred from genetic interaction PubMed 18762576. Source: MGI

   Cellular_componentextracellular space

Inferred from direct assay PubMed 18762576. Source: MGI

   Molecular_functioninsulin-like growth factor receptor binding

Inferred from physical interaction PubMed 14566968. Source: MGI

protein binding

Inferred from physical interaction PubMed 14566968PubMed 19706595. Source: MGI

protein serine/threonine kinase activator activity

Inferred from mutant phenotype PubMed 20032056. Source: BHF-UCL

receptor activator activity

Inferred from mutant phenotype PubMed 20032056. Source: BHF-UCL

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2424
Chain25 – 9167Insulin-like growth factor II
PRO_0000015720
Propeptide92 – 18089E peptide
PRO_0000015721
Peptide93 – 12634Preptin Ref.7
PRO_0000370377

Regions

Region25 – 5228B
Region53 – 6412C
Region65 – 8521A
Region86 – 916D

Amino acid modifications

Disulfide bond33 ↔ 71 By similarity
Disulfide bond45 ↔ 84 By similarity
Disulfide bond70 ↔ 75 By similarity

Sequences

Sequence LengthMass (Da)Tools
P09535 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 01730F8856EE6D7B

FASTA18020,030
        10         20         30         40         50         60 
MGIPVGKSML VLLISLAFAL CCIAAYGPGE TLCGGELVDT LQFVCSDRGF YFSRPSSRAN 

        70         80         90        100        110        120 
RRSRGIVEEC CFRSCDLALL ETYCATPAKS ERDVSTSQAV LPDDFPRYPV GKFFQYDTWR 

       130        140        150        160        170        180 
QSAGRLRRGL PALLRARRGR MLAKELKEFR EAKRHRPLIV LPPKDPAHGG ASSEMSSNHQ 

« Hide

References

« Hide 'large scale' references
[1]"Sequence of a placental cDNA encoding the mouse insulin-like growth factor II precursor."
Stempien M.M., Fong N.M., Rall L.B., Bell G.I.
DNA 5:357-361(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"Evolution of insulin-like growth factor II: characterization of the mouse IGF-II gene and identification of two pseudo-exons."
Rotwein P., Hall L.J.
DNA Cell Biol. 9:725-735(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Nucleotide sequence of a 28-kb mouse genomic region comprising the imprinted Igf2 gene."
Sasaki H., Shimozaki K., Zubair M., Aoki N., Hatano N., Moore T., Feil R., Constancia M., Reik W., Rotwein P.
DNA Res. 3:331-335(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[4]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Embryo.
[5]"Coordinate expression of insulin-like growth factor II and its receptor during muscle differentiation."
Tollefsen S.E., Sadow J.L., Rotwein P.
Proc. Natl. Acad. Sci. U.S.A. 86:1543-1547(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-52.
[6]"Differential expression of the human, mouse and rat IGF-II genes."
Holthuizen P.E., Cleutjens C.B., Veenstra G.J., van der Lee F.M., Koonen-Reemst A.M., Sussenbach J.S.
Regul. Pept. 48:77-89(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-52 AND 103-180.
Strain: BALB/c.
Tissue: Spleen.
[7]"Preptin derived from proinsulin-like growth factor II (proIGF-II) is secreted from pancreatic islet beta-cells and enhances insulin secretion."
Buchanan C.M., Phillips A.R., Cooper G.J.
Biochem. J. 360:431-439(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 93-126, INSULIN-ENHANCING FUNCTION OF PREPTIN.
[8]"Preptin, another peptide product of the pancreatic beta-cell, is osteogenic in vitro and in vivo."
Cornish J., Callon K.E., Bava U., Watson M., Xu X., Lin J.M., Chan V.A., Grey A.B., Naot D., Buchanan C.M., Cooper G.J., Reid I.R.
Am. J. Physiol. 292:E117-E122(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: OSTEOGENIC FUNCTION OF PREPTIN.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M14951 mRNA. Translation: AAA37683.1.
M36332, M36331 Genomic DNA. Translation: AAA37926.1.
U71085 Genomic DNA. Translation: AAC53516.1.
BC053489 mRNA. Translation: AAH53489.1.
M24633 Genomic DNA. Translation: AAA37923.1.
X71921 Genomic DNA. Translation: CAA50737.1.
X71922 Genomic DNA. Translation: CAA50738.1.
CCDSCCDS52458.1.
PIRA24913.
RefSeqNP_001116208.1. NM_001122736.1.
NP_001116209.1. NM_001122737.1.
XP_006508550.1. XM_006508487.1.
UniGeneMm.3862.

3D structure databases

ProteinModelPortalP09535.
SMRP09535. Positions 30-88.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid200549. 2 interactions.

PTM databases

PhosphoSiteP09535.

Proteomic databases

MaxQBP09535.
PaxDbP09535.
PRIDEP09535.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000000033; ENSMUSP00000000033; ENSMUSG00000048583.
ENSMUST00000097936; ENSMUSP00000095549; ENSMUSG00000048583.
ENSMUST00000105935; ENSMUSP00000101555; ENSMUSG00000048583.
ENSMUST00000105936; ENSMUSP00000101556; ENSMUSG00000048583.
GeneID16002.
KEGGmmu:16002.
UCSCuc009kod.2. mouse.

Organism-specific databases

CTD3481.
MGIMGI:96434. Igf2.

Phylogenomic databases

eggNOGNOG44017.
GeneTreeENSGT00530000063856.
HOGENOMHOG000233362.
HOVERGENHBG006137.
InParanoidP09535.
KOK13769.
OrthoDBEOG7TF7CG.
PhylomeDBP09535.
TreeFamTF332820.

Gene expression databases

ArrayExpressP09535.
BgeeP09535.
CleanExMM_IGF2.
GenevestigatorP09535.

Family and domain databases

Gene3D1.10.100.10. 1 hit.
InterProIPR022334. IGF2.
IPR013576. IGF2_C.
IPR016179. Insulin-like.
IPR022350. Insulin-like_growth_factor.
IPR022353. Insulin_CS.
IPR022352. Insulin_family.
[Graphical view]
PfamPF08365. IGF2_C. 1 hit.
PF00049. Insulin. 1 hit.
[Graphical view]
PRINTSPR02002. INSLNLIKEGF.
PR02006. INSLNLIKEGF2.
PR00276. INSULINFAMLY.
ProDomPD005188. IGF2_C. 1 hit.
[Graphical view] [Entries sharing at least one domain]
SMARTSM00078. IlGF. 1 hit.
[Graphical view]
SUPFAMSSF56994. SSF56994. 1 hit.
PROSITEPS00262. INSULIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSIGF2. mouse.
NextBio288794.
PROP09535.
SOURCESearch...

Entry information

Entry nameIGF2_MOUSE
AccessionPrimary (citable) accession number: P09535
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: July 9, 2014
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot