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P09535

- IGF2_MOUSE

UniProt

P09535 - IGF2_MOUSE

Protein

Insulin-like growth factor II

Gene

Igf2

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 130 (01 Oct 2014)
      Sequence version 1 (01 Jul 1989)
      Previous versions | rss
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    Functioni

    The insulin-like growth factors possess growth-promoting activity. In vitro, they are potent mitogens for cultured cells. IGF-II is influenced by placental lactogen and may play a role in fetal development.
    Preptin undergoes glucose-mediated co-secretion with insulin, and acts as physiological amplifier of glucose-mediated insulin secretion. Exhibits osteogenic properties by increasing osteoblast mitogenic activity through phosphoactivation of MAPK1 and MAPK3.

    GO - Molecular functioni

    1. insulin-like growth factor receptor binding Source: MGI
    2. protein binding Source: MGI
    3. protein serine/threonine kinase activator activity Source: BHF-UCL
    4. receptor activator activity Source: BHF-UCL

    GO - Biological processi

    1. exocrine pancreas development Source: MGI
    2. glucose metabolic process Source: UniProtKB-KW
    3. insulin receptor signaling pathway via phosphatidylinositol 3-kinase Source: BHF-UCL
    4. organ morphogenesis Source: MGI
    5. osteoblast differentiation Source: BHF-UCL
    6. positive regulation of catalytic activity Source: BHF-UCL
    7. positive regulation of cell division Source: UniProtKB-KW
    8. positive regulation of glycogen (starch) synthase activity Source: BHF-UCL
    9. positive regulation of glycogen biosynthetic process Source: BHF-UCL
    10. positive regulation of insulin receptor signaling pathway Source: BHF-UCL
    11. positive regulation of peptidyl-tyrosine phosphorylation Source: BHF-UCL
    12. positive regulation of protein phosphorylation Source: BHF-UCL
    13. positive regulation of protein serine/threonine kinase activity Source: GOC
    14. positive regulation of receptor activity Source: GOC
    15. positive regulation of transcription from RNA polymerase II promoter Source: MGI
    16. striated muscle cell differentiation Source: MGI

    Keywords - Molecular functioni

    Growth factor, Hormone, Mitogen

    Keywords - Biological processi

    Carbohydrate metabolism, Glucose metabolism, Osteogenesis

    Enzyme and pathway databases

    ReactomeiREACT_188215. IRS-related events triggered by IGF1R.
    REACT_188219. Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R).
    REACT_188220. SHC-related events triggered by IGF1R.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Insulin-like growth factor II
    Alternative name(s):
    IGF-II
    Multiplication-stimulating polypeptide
    Cleaved into the following 2 chains:
    Gene namesi
    Name:Igf2
    Synonyms:Igf-2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 7

    Organism-specific databases

    MGIiMGI:96434. Igf2.

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular space Source: MGI

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2424Add
    BLAST
    Chaini25 – 9167Insulin-like growth factor IIPRO_0000015720Add
    BLAST
    Propeptidei92 – 18089E peptidePRO_0000015721Add
    BLAST
    Peptidei93 – 12634PreptinPRO_0000370377Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi33 ↔ 71By similarity
    Disulfide bondi45 ↔ 84By similarity
    Disulfide bondi70 ↔ 75By similarity

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond

    Proteomic databases

    MaxQBiP09535.
    PaxDbiP09535.
    PRIDEiP09535.

    PTM databases

    PhosphoSiteiP09535.

    Expressioni

    Developmental stagei

    Low levels of expression during myoblast proliferation. Levels rise rapidly during myoblast differentiation and then decrease.

    Gene expression databases

    ArrayExpressiP09535.
    BgeeiP09535.
    CleanExiMM_IGF2.
    GenevestigatoriP09535.

    Interactioni

    Protein-protein interaction databases

    BioGridi200549. 2 interactions.

    Structurei

    3D structure databases

    ProteinModelPortaliP09535.
    SMRiP09535. Positions 30-88.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni25 – 5228BAdd
    BLAST
    Regioni53 – 6412CAdd
    BLAST
    Regioni65 – 8521AAdd
    BLAST
    Regioni86 – 916D

    Sequence similaritiesi

    Belongs to the insulin family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG44017.
    GeneTreeiENSGT00530000063856.
    HOGENOMiHOG000233362.
    HOVERGENiHBG006137.
    InParanoidiP09535.
    KOiK13769.
    OrthoDBiEOG7TF7CG.
    PhylomeDBiP09535.
    TreeFamiTF332820.

    Family and domain databases

    Gene3Di1.10.100.10. 1 hit.
    InterProiIPR022334. IGF2.
    IPR013576. IGF2_C.
    IPR016179. Insulin-like.
    IPR022350. Insulin-like_growth_factor.
    IPR022353. Insulin_CS.
    IPR022352. Insulin_family.
    [Graphical view]
    PfamiPF08365. IGF2_C. 1 hit.
    PF00049. Insulin. 1 hit.
    [Graphical view]
    PRINTSiPR02002. INSLNLIKEGF.
    PR02006. INSLNLIKEGF2.
    PR00276. INSULINFAMLY.
    ProDomiPD005188. IGF2_C. 1 hit.
    [Graphical view] [Entries sharing at least one domain]
    SMARTiSM00078. IlGF. 1 hit.
    [Graphical view]
    SUPFAMiSSF56994. SSF56994. 1 hit.
    PROSITEiPS00262. INSULIN. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P09535-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGIPVGKSML VLLISLAFAL CCIAAYGPGE TLCGGELVDT LQFVCSDRGF    50
    YFSRPSSRAN RRSRGIVEEC CFRSCDLALL ETYCATPAKS ERDVSTSQAV 100
    LPDDFPRYPV GKFFQYDTWR QSAGRLRRGL PALLRARRGR MLAKELKEFR 150
    EAKRHRPLIV LPPKDPAHGG ASSEMSSNHQ 180
    Length:180
    Mass (Da):20,030
    Last modified:July 1, 1989 - v1
    Checksum:i01730F8856EE6D7B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M14951 mRNA. Translation: AAA37683.1.
    M36332, M36331 Genomic DNA. Translation: AAA37926.1.
    U71085 Genomic DNA. Translation: AAC53516.1.
    BC053489 mRNA. Translation: AAH53489.1.
    M24633 Genomic DNA. Translation: AAA37923.1.
    X71921 Genomic DNA. Translation: CAA50737.1.
    X71922 Genomic DNA. Translation: CAA50738.1.
    CCDSiCCDS52458.1.
    PIRiA24913.
    RefSeqiNP_001116208.1. NM_001122736.1.
    NP_001116209.1. NM_001122737.1.
    XP_006508550.1. XM_006508487.1.
    UniGeneiMm.3862.

    Genome annotation databases

    EnsembliENSMUST00000000033; ENSMUSP00000000033; ENSMUSG00000048583.
    ENSMUST00000097936; ENSMUSP00000095549; ENSMUSG00000048583.
    ENSMUST00000105935; ENSMUSP00000101555; ENSMUSG00000048583.
    ENSMUST00000105936; ENSMUSP00000101556; ENSMUSG00000048583.
    GeneIDi16002.
    KEGGimmu:16002.
    UCSCiuc009kod.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M14951 mRNA. Translation: AAA37683.1 .
    M36332 , M36331 Genomic DNA. Translation: AAA37926.1 .
    U71085 Genomic DNA. Translation: AAC53516.1 .
    BC053489 mRNA. Translation: AAH53489.1 .
    M24633 Genomic DNA. Translation: AAA37923.1 .
    X71921 Genomic DNA. Translation: CAA50737.1 .
    X71922 Genomic DNA. Translation: CAA50738.1 .
    CCDSi CCDS52458.1.
    PIRi A24913.
    RefSeqi NP_001116208.1. NM_001122736.1.
    NP_001116209.1. NM_001122737.1.
    XP_006508550.1. XM_006508487.1.
    UniGenei Mm.3862.

    3D structure databases

    ProteinModelPortali P09535.
    SMRi P09535. Positions 30-88.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 200549. 2 interactions.

    PTM databases

    PhosphoSitei P09535.

    Proteomic databases

    MaxQBi P09535.
    PaxDbi P09535.
    PRIDEi P09535.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000000033 ; ENSMUSP00000000033 ; ENSMUSG00000048583 .
    ENSMUST00000097936 ; ENSMUSP00000095549 ; ENSMUSG00000048583 .
    ENSMUST00000105935 ; ENSMUSP00000101555 ; ENSMUSG00000048583 .
    ENSMUST00000105936 ; ENSMUSP00000101556 ; ENSMUSG00000048583 .
    GeneIDi 16002.
    KEGGi mmu:16002.
    UCSCi uc009kod.2. mouse.

    Organism-specific databases

    CTDi 3481.
    MGIi MGI:96434. Igf2.

    Phylogenomic databases

    eggNOGi NOG44017.
    GeneTreei ENSGT00530000063856.
    HOGENOMi HOG000233362.
    HOVERGENi HBG006137.
    InParanoidi P09535.
    KOi K13769.
    OrthoDBi EOG7TF7CG.
    PhylomeDBi P09535.
    TreeFami TF332820.

    Enzyme and pathway databases

    Reactomei REACT_188215. IRS-related events triggered by IGF1R.
    REACT_188219. Signaling by Type 1 Insulin-like Growth Factor 1 Receptor (IGF1R).
    REACT_188220. SHC-related events triggered by IGF1R.

    Miscellaneous databases

    ChiTaRSi IGF2. mouse.
    NextBioi 288794.
    PROi P09535.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P09535.
    Bgeei P09535.
    CleanExi MM_IGF2.
    Genevestigatori P09535.

    Family and domain databases

    Gene3Di 1.10.100.10. 1 hit.
    InterProi IPR022334. IGF2.
    IPR013576. IGF2_C.
    IPR016179. Insulin-like.
    IPR022350. Insulin-like_growth_factor.
    IPR022353. Insulin_CS.
    IPR022352. Insulin_family.
    [Graphical view ]
    Pfami PF08365. IGF2_C. 1 hit.
    PF00049. Insulin. 1 hit.
    [Graphical view ]
    PRINTSi PR02002. INSLNLIKEGF.
    PR02006. INSLNLIKEGF2.
    PR00276. INSULINFAMLY.
    ProDomi PD005188. IGF2_C. 1 hit.
    [Graphical view ] [Entries sharing at least one domain ]
    SMARTi SM00078. IlGF. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56994. SSF56994. 1 hit.
    PROSITEi PS00262. INSULIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Sequence of a placental cDNA encoding the mouse insulin-like growth factor II precursor."
      Stempien M.M., Fong N.M., Rall L.B., Bell G.I.
      DNA 5:357-361(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. "Evolution of insulin-like growth factor II: characterization of the mouse IGF-II gene and identification of two pseudo-exons."
      Rotwein P., Hall L.J.
      DNA Cell Biol. 9:725-735(1990) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    3. "Nucleotide sequence of a 28-kb mouse genomic region comprising the imprinted Igf2 gene."
      Sasaki H., Shimozaki K., Zubair M., Aoki N., Hatano N., Moore T., Feil R., Constancia M., Reik W., Rotwein P.
      DNA Res. 3:331-335(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Embryo.
    5. "Coordinate expression of insulin-like growth factor II and its receptor during muscle differentiation."
      Tollefsen S.E., Sadow J.L., Rotwein P.
      Proc. Natl. Acad. Sci. U.S.A. 86:1543-1547(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-52.
    6. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-52 AND 103-180.
      Strain: BALB/c.
      Tissue: Spleen.
    7. "Preptin derived from proinsulin-like growth factor II (proIGF-II) is secreted from pancreatic islet beta-cells and enhances insulin secretion."
      Buchanan C.M., Phillips A.R., Cooper G.J.
      Biochem. J. 360:431-439(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 93-126, INSULIN-ENHANCING FUNCTION OF PREPTIN.
    8. "Preptin, another peptide product of the pancreatic beta-cell, is osteogenic in vitro and in vivo."
      Cornish J., Callon K.E., Bava U., Watson M., Xu X., Lin J.M., Chan V.A., Grey A.B., Naot D., Buchanan C.M., Cooper G.J., Reid I.R.
      Am. J. Physiol. 292:E117-E122(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: OSTEOGENIC FUNCTION OF PREPTIN.

    Entry informationi

    Entry nameiIGF2_MOUSE
    AccessioniPrimary (citable) accession number: P09535
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 130 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3