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Protein

Ferritin heavy chain

Gene

Fth1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Stores iron in a soluble, non-toxic, readily available form. Important for iron homeostasis. Has ferroxidase activity. Iron is taken up in the ferrous form and deposited as ferric hydroxides after oxidation. Also plays a role in delivery of iron to cells. Mediates iron uptake in capsule cells of the developing kidney.1 Publication

Catalytic activityi

4 Fe2+ + 4 H+ + O2 = 4 Fe3+ + 2 H2O.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi28Iron 1PROSITE-ProRule annotation1
Metal bindingi63Iron 1PROSITE-ProRule annotation1
Metal bindingi63Iron 2PROSITE-ProRule annotation1
Metal bindingi66Iron 1PROSITE-ProRule annotation1
Metal bindingi108Iron 2PROSITE-ProRule annotation1
Metal bindingi142Iron 2PROSITE-ProRule annotation1

GO - Molecular functioni

  • ferric iron binding Source: InterPro
  • ferroxidase activity Source: UniProtKB-EC
  • iron ion binding Source: MGI

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Iron storage

Keywords - Ligandi

Iron, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-3000480. Scavenging by Class A Receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
Ferritin heavy chain (EC:1.16.3.1)
Short name:
Ferritin H subunit
Cleaved into the following chain:
Gene namesi
Name:Fth1
Synonyms:Fth
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:95588. Fth1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Homozygous mutant embryos die in utero between 3.5 and 9.5 dpc (PubMed:10652280). Heterozygous animals are healthy and fertile and do not present any apparent abnormalities. They show slightly elevated tissue light chain ferritin content and 7- to 10-fold more light chain ferritin in the serum than normal mice, but their serum iron remains unchanged.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002010491 – 182Ferritin heavy chainAdd BLAST182
Initiator methionineiRemoved; alternateBy similarity
ChainiPRO_00004244732 – 182Ferritin heavy chain, N-terminally processedAdd BLAST181

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei2N-acetylthreonine; in Ferritin heavy chain, N-terminally processedBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP09528.
MaxQBiP09528.
PaxDbiP09528.
PeptideAtlasiP09528.
PRIDEiP09528.

2D gel databases

REPRODUCTION-2DPAGEP09528.

PTM databases

iPTMnetiP09528.
PhosphoSitePlusiP09528.
SwissPalmiP09528.

Expressioni

Developmental stagei

At 9.5 dpc, detected at low levels in the developing heart and central nervous system. At later stages of development, widely expressed, predominantly in the heart and brown fat tissue.1 Publication

Gene expression databases

BgeeiENSMUSG00000024661.
CleanExiMM_FTH1.
GenevisibleiP09528. MM.

Interactioni

Subunit structurei

Oligomer of 24 subunits. There are two types of subunits: L (light) chain and H (heavy) chain. The major chain can be light or heavy, depending on the species and tissue type. The functional molecule forms a roughly spherical shell with a diameter of 12 nm and contains a central cavity into which the insoluble mineral iron core is deposited.

Protein-protein interaction databases

IntActiP09528. 5 interactors.
MINTiMINT-1856685.
STRINGi10090.ENSMUSP00000025563.

Structurei

Secondary structure

1182
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi15 – 43Combined sources29
Turni45 – 47Combined sources3
Helixi50 – 76Combined sources27
Helixi97 – 124Combined sources28
Helixi128 – 137Combined sources10
Helixi139 – 158Combined sources20
Turni159 – 163Combined sources5
Helixi166 – 174Combined sources9

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3WNWX-ray2.24A/B/C/D/E/F/G/H/I/J/K/L1-182[»]
ProteinModelPortaliP09528.
SMRiP09528.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini11 – 160Ferritin-like diironPROSITE-ProRule annotationAdd BLAST150

Sequence similaritiesi

Belongs to the ferritin family.Curated
Contains 1 ferritin-like diiron domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG2332. Eukaryota.
COG1528. LUCA.
GeneTreeiENSGT00760000119129.
HOGENOMiHOG000223383.
HOVERGENiHBG000410.
InParanoidiP09528.
KOiK00522.
OMAiFMEYQNQ.
OrthoDBiEOG091G0J20.
PhylomeDBiP09528.
TreeFamiTF313885.

Family and domain databases

Gene3Di1.20.1260.10. 1 hit.
InterProiIPR001519. Ferritin.
IPR009040. Ferritin-like_diiron.
IPR009078. Ferritin-like_SF.
IPR012347. Ferritin-rel.
IPR014034. Ferritin_CS.
IPR008331. Ferritin_DPS_dom.
[Graphical view]
PANTHERiPTHR11431. PTHR11431. 1 hit.
PfamiPF00210. Ferritin. 1 hit.
[Graphical view]
SUPFAMiSSF47240. SSF47240. 1 hit.
PROSITEiPS00540. FERRITIN_1. 1 hit.
PS00204. FERRITIN_2. 1 hit.
PS50905. FERRITIN_LIKE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09528-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTASPSQVR QNYHQDAEAA INRQINLELY ASYVYLSMSC YFDRDDVALK
60 70 80 90 100
NFAKYFLHQS HEEREHAEKL MKLQNQRGGR IFLQDIKKPD RDDWESGLNA
110 120 130 140 150
MECALHLEKS VNQSLLELHK LATDKNDPHL CDFIETYYLS EQVKSIKELG
160 170 180
DHVTNLRKMG APEAGMAEYL FDKHTLGHGD ES
Length:182
Mass (Da):21,067
Last modified:January 23, 2007 - v2
Checksum:i129A8887A2BC650B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti17A → S in AAA37612 (PubMed:2708374).Curated1
Sequence conflicti137Y → H in AAA37612 (PubMed:2708374).Curated1
Sequence conflicti140S → N in AAA37612 (PubMed:2708374).Curated1
Sequence conflicti164A → S in AAA37612 (PubMed:2708374).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52561 Genomic DNA. Translation: CAA36795.1.
X12812 mRNA. Translation: CAA31300.1.
J03941 mRNA. Translation: AAA37611.1.
M60170 Genomic DNA. Translation: AAA37613.1.
M24509 mRNA. Translation: AAA37612.1.
AK027998 mRNA. Translation: BAC25694.1.
AK139622 mRNA. Translation: BAE24084.1.
AK147082 mRNA. Translation: BAE27662.1.
AK150262 mRNA. Translation: BAE29419.1.
AK150508 mRNA. Translation: BAE29621.1.
AK150628 mRNA. Translation: BAE29718.1.
AK150679 mRNA. Translation: BAE29759.1.
AK150693 mRNA. Translation: BAE29772.1.
AK151192 mRNA. Translation: BAE30189.1.
AK151241 mRNA. Translation: BAE30233.1.
AK151399 mRNA. Translation: BAE30367.1.
AK151609 mRNA. Translation: BAE30548.1.
AK151675 mRNA. Translation: BAE30600.1.
AK152071 mRNA. Translation: BAE30924.1.
AK152542 mRNA. Translation: BAE31297.1.
AK152702 mRNA. Translation: BAE31431.1.
AK153017 mRNA. Translation: BAE31651.1.
AK153195 mRNA. Translation: BAE31795.1.
AK153199 mRNA. Translation: BAE31799.1.
AK159243 mRNA. Translation: BAE34925.1.
AK168601 mRNA. Translation: BAE40468.1.
AK169004 mRNA. Translation: BAE40803.1.
BC012314 mRNA. Translation: AAH12314.1.
CCDSiCCDS29567.1.
PIRiS06070.
RefSeqiNP_034369.1. NM_010239.2.
UniGeneiMm.1776.

Genome annotation databases

EnsembliENSMUST00000025563; ENSMUSP00000025563; ENSMUSG00000024661.
GeneIDi14319.
KEGGimmu:14319.
UCSCiuc008got.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X52561 Genomic DNA. Translation: CAA36795.1.
X12812 mRNA. Translation: CAA31300.1.
J03941 mRNA. Translation: AAA37611.1.
M60170 Genomic DNA. Translation: AAA37613.1.
M24509 mRNA. Translation: AAA37612.1.
AK027998 mRNA. Translation: BAC25694.1.
AK139622 mRNA. Translation: BAE24084.1.
AK147082 mRNA. Translation: BAE27662.1.
AK150262 mRNA. Translation: BAE29419.1.
AK150508 mRNA. Translation: BAE29621.1.
AK150628 mRNA. Translation: BAE29718.1.
AK150679 mRNA. Translation: BAE29759.1.
AK150693 mRNA. Translation: BAE29772.1.
AK151192 mRNA. Translation: BAE30189.1.
AK151241 mRNA. Translation: BAE30233.1.
AK151399 mRNA. Translation: BAE30367.1.
AK151609 mRNA. Translation: BAE30548.1.
AK151675 mRNA. Translation: BAE30600.1.
AK152071 mRNA. Translation: BAE30924.1.
AK152542 mRNA. Translation: BAE31297.1.
AK152702 mRNA. Translation: BAE31431.1.
AK153017 mRNA. Translation: BAE31651.1.
AK153195 mRNA. Translation: BAE31795.1.
AK153199 mRNA. Translation: BAE31799.1.
AK159243 mRNA. Translation: BAE34925.1.
AK168601 mRNA. Translation: BAE40468.1.
AK169004 mRNA. Translation: BAE40803.1.
BC012314 mRNA. Translation: AAH12314.1.
CCDSiCCDS29567.1.
PIRiS06070.
RefSeqiNP_034369.1. NM_010239.2.
UniGeneiMm.1776.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3WNWX-ray2.24A/B/C/D/E/F/G/H/I/J/K/L1-182[»]
ProteinModelPortaliP09528.
SMRiP09528.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP09528. 5 interactors.
MINTiMINT-1856685.
STRINGi10090.ENSMUSP00000025563.

PTM databases

iPTMnetiP09528.
PhosphoSitePlusiP09528.
SwissPalmiP09528.

2D gel databases

REPRODUCTION-2DPAGEP09528.

Proteomic databases

EPDiP09528.
MaxQBiP09528.
PaxDbiP09528.
PeptideAtlasiP09528.
PRIDEiP09528.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025563; ENSMUSP00000025563; ENSMUSG00000024661.
GeneIDi14319.
KEGGimmu:14319.
UCSCiuc008got.2. mouse.

Organism-specific databases

CTDi2495.
MGIiMGI:95588. Fth1.

Phylogenomic databases

eggNOGiKOG2332. Eukaryota.
COG1528. LUCA.
GeneTreeiENSGT00760000119129.
HOGENOMiHOG000223383.
HOVERGENiHBG000410.
InParanoidiP09528.
KOiK00522.
OMAiFMEYQNQ.
OrthoDBiEOG091G0J20.
PhylomeDBiP09528.
TreeFamiTF313885.

Enzyme and pathway databases

ReactomeiR-MMU-3000480. Scavenging by Class A Receptors.

Miscellaneous databases

ChiTaRSiFth1. mouse.
PROiP09528.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024661.
CleanExiMM_FTH1.
GenevisibleiP09528. MM.

Family and domain databases

Gene3Di1.20.1260.10. 1 hit.
InterProiIPR001519. Ferritin.
IPR009040. Ferritin-like_diiron.
IPR009078. Ferritin-like_SF.
IPR012347. Ferritin-rel.
IPR014034. Ferritin_CS.
IPR008331. Ferritin_DPS_dom.
[Graphical view]
PANTHERiPTHR11431. PTHR11431. 1 hit.
PfamiPF00210. Ferritin. 1 hit.
[Graphical view]
SUPFAMiSSF47240. SSF47240. 1 hit.
PROSITEiPS00540. FERRITIN_1. 1 hit.
PS00204. FERRITIN_2. 1 hit.
PS50905. FERRITIN_LIKE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFRIH_MOUSE
AccessioniPrimary (citable) accession number: P09528
Secondary accession number(s): Q3UI44
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 162 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.