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P09527

- RAB7A_RAT

UniProt

P09527 - RAB7A_RAT

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Protein

Ras-related protein Rab-7a

Gene
Rab7a, Rab7
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Key regulator in endo-lysosomal trafficking. Governs early-to-late endosomal maturation, microtubule minus-end as well as plus-end directed endosomal migration and positioning, and endosome-lysosome transport through different protein-protein interaction cascades. Plays a central role, not only in endosomal traffic, but also in many other cellular and physiological events, such as growth-factor-mediated cell signaling, nutrient-transportor mediated nutrient uptake, neurotrophin transport in the axons of neurons and lipid metabolism. Also involved in regulation of some specialized endosomal membrane trafficking, such as maturation of melanosomes, pathogen-induced phagosomes (or vacuoles) and autophagosomes. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and Mycobacteria. Plays important roles in microbial pathogen infection and survival, as well as in participating in the life cycle of viruses. Microbial pathogens possess survival strategies governed by RAB7A, sometimes by employing RAB7A function (e.g. Salmonella) and sometimes by excluding RAB7A function (e.g. Mycobacterium). In concert with RAC1, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. Controls the endosomal trafficking and neurite outgrowth signaling of NTRK1/TRKA. Regulates the endocytic trafficking of the EGF-EGFR complex by regulating its lysosomal degradation.2 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi15 – 228GTP
Nucleotide bindingi34 – 407GTP
Nucleotide bindingi63 – 675GTP
Nucleotide bindingi125 – 1284GTP
Nucleotide bindingi156 – 1572GTP

GO - Molecular functioni

  1. GDP binding Source: RGD
  2. GTPase activity Source: RGD
  3. GTP binding Source: RGD
  4. protein binding Source: UniProtKB
  5. Rac GTPase binding Source: RGD

GO - Biological processi

  1. bone resorption Source: RGD
  2. early endosome to late endosome transport Source: UniProtKB
  3. endosome to lysosome transport Source: Ensembl
  4. epidermal growth factor catabolic process Source: Ensembl
  5. phagosome acidification Source: UniProtKB
  6. phagosome-lysosome fusion Source: UniProtKB
  7. protein targeting to lysosome Source: Ensembl
  8. small GTPase mediated signal transduction Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Keywords - Ligandi

GTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_198830. MHC class II antigen presentation.

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-7a
Alternative name(s):
Ras-related protein BRL-RAS
Ras-related protein p23
Gene namesi
Name:Rab7a
Synonyms:Rab7
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 4

Organism-specific databases

RGDi61908. Rab7a.

Subcellular locationi

Late endosome. Lysosome. Cytoplasmic vesiclephagosome By similarity. Cytoplasmic vesiclephagosome membrane; Lipid-anchor; Cytoplasmic side By similarity. Melanosome By similarity
Note: Colocalizes with OSBPL1A at the late endosome. Recruited to phagosomes containing S.aureus or Mycobacterium By similarity. Found in the ruffled border (a late endosomal-like compartment in the plasma membrane) of bone-resorbing osteoclasts.1 Publication

GO - Cellular componenti

  1. alveolar lamellar body Source: RGD
  2. cytoplasmic vesicle Source: RGD
  3. Golgi apparatus Source: Ensembl
  4. late endosome Source: RGD
  5. lysosome Source: UniProtKB-SubCell
  6. melanosome Source: UniProtKB-SubCell
  7. phagocytic vesicle Source: UniProtKB
  8. phagocytic vesicle membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Endosome, Lysosome, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 207207Ras-related protein Rab-7aPRO_0000121124Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei72 – 721Phosphoserine By similarity
Lipidationi205 – 2051S-geranylgeranyl cysteine By similarity
Modified residuei207 – 2071Cysteine methyl ester By similarity
Lipidationi207 – 2071S-geranylgeranyl cysteine By similarity

Keywords - PTMi

Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

PaxDbiP09527.
PRIDEiP09527.

PTM databases

PhosphoSiteiP09527.

Expressioni

Tissue specificityi

Expressed in osteoclasts and in neurons.2 Publications

Gene expression databases

GenevestigatoriP09527.

Interactioni

Subunit structurei

Interacts with RILP, PSMA7, RNF115 and FYCO1. Interacts with the PIK3C3/VPS34-PIK3R4 complex. The GTP-bound form interacts with OSBPL1A and RAC1 By similarity. Interacts with NTRK1/TRKA. The GTP-bound form interacts with RAC1. Interacts with CLN3 By similarity. Interacts with C9orf72 By similarity. Interacts with CHM, the substrate-binding subunit of the Rab geranylgeranyltransferase complex.3 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
ChmP377272EBI-916225,EBI-1039231

Protein-protein interaction databases

BioGridi248093. 1 interaction.
IntActiP09527. 5 interactions.
MINTiMINT-1775656.
STRINGi10116.ENSRNOP00000016432.

Structurei

Secondary structure

1
207
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi8 – 147
Helixi21 – 3010
Beta strandi42 – 5413
Beta strandi56 – 649
Helixi68 – 703
Helixi76 – 783
Beta strandi82 – 898
Helixi93 – 975
Helixi99 – 11012
Helixi115 – 1173
Beta strandi120 – 1256
Beta strandi129 – 1313
Helixi136 – 14510
Beta strandi151 – 1533
Turni156 – 1594
Helixi162 – 18524

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1VG0X-ray2.20B1-207[»]
1VG1X-ray1.90A1-185[»]
1VG8X-ray1.70A/B/C/D1-207[»]
1VG9X-ray2.50B/D/F/H1-185[»]
ProteinModelPortaliP09527.
SMRiP09527. Positions 7-190.

Miscellaneous databases

EvolutionaryTraceiP09527.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi37 – 459Effector region By similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1100.
GeneTreeiENSGT00700000104345.
HOGENOMiHOG000233968.
HOVERGENiHBG009351.
InParanoidiP09527.
KOiK07897.
OMAiDYPDPIK.
OrthoDBiEOG7G4QG8.
PhylomeDBiP09527.
TreeFamiTF105605.

Family and domain databases

Gene3Di3.40.50.300. 1 hit.
InterProiIPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]
PfamiPF00071. Ras. 1 hit.
[Graphical view]
PRINTSiPR00449. RASTRNSFRMNG.
SMARTiSM00175. RAB. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 1 hit.
TIGRFAMsiTIGR00231. small_GTP. 1 hit.
PROSITEiPS51419. RAB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P09527-1 [UniParc]FASTAAdd to Basket

« Hide

MTSRKKVLLK VIILGDSGVG KTSLMNQYVN KKFSNQYKAT IGADFLTKEV    50
MVDDRLVTMQ IWDTAGQERF QSLGVAFYRG ADCCVLVFDV TAPNTFKTLD 100
SWRDEFLIQA SPRDPENFPF VVLGNKIDLE NRQVATKRAQ AWCYSKNNIP 150
YFETSAKEAI NVEQAFQTIA RNALKQETEV ELYNEFPEPI KLDKNERAKA 200
SAESCSC 207
Length:207
Mass (Da):23,504
Last modified:October 1, 1996 - v2
Checksum:iA2AF33B02F672971
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X12535 mRNA. Translation: CAA31053.1.
AF286535 mRNA. Translation: AAG00543.1.
AB158427 mRNA. Translation: BAE16999.1.
AB158428 mRNA. Translation: BAE17000.1.
BC072470 mRNA. Translation: AAH72470.1.
PIRiS01934.
RefSeqiNP_076440.1. NM_023950.3.
XP_006236911.1. XM_006236849.1.
UniGeneiRn.1425.

Genome annotation databases

EnsembliENSRNOT00000016432; ENSRNOP00000016432; ENSRNOG00000012247.
GeneIDi29448.
KEGGirno:29448.
UCSCiRGD:61908. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X12535 mRNA. Translation: CAA31053.1 .
AF286535 mRNA. Translation: AAG00543.1 .
AB158427 mRNA. Translation: BAE16999.1 .
AB158428 mRNA. Translation: BAE17000.1 .
BC072470 mRNA. Translation: AAH72470.1 .
PIRi S01934.
RefSeqi NP_076440.1. NM_023950.3.
XP_006236911.1. XM_006236849.1.
UniGenei Rn.1425.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1VG0 X-ray 2.20 B 1-207 [» ]
1VG1 X-ray 1.90 A 1-185 [» ]
1VG8 X-ray 1.70 A/B/C/D 1-207 [» ]
1VG9 X-ray 2.50 B/D/F/H 1-185 [» ]
ProteinModelPortali P09527.
SMRi P09527. Positions 7-190.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 248093. 1 interaction.
IntActi P09527. 5 interactions.
MINTi MINT-1775656.
STRINGi 10116.ENSRNOP00000016432.

PTM databases

PhosphoSitei P09527.

Proteomic databases

PaxDbi P09527.
PRIDEi P09527.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000016432 ; ENSRNOP00000016432 ; ENSRNOG00000012247 .
GeneIDi 29448.
KEGGi rno:29448.
UCSCi RGD:61908. rat.

Organism-specific databases

CTDi 7879.
RGDi 61908. Rab7a.

Phylogenomic databases

eggNOGi COG1100.
GeneTreei ENSGT00700000104345.
HOGENOMi HOG000233968.
HOVERGENi HBG009351.
InParanoidi P09527.
KOi K07897.
OMAi DYPDPIK.
OrthoDBi EOG7G4QG8.
PhylomeDBi P09527.
TreeFami TF105605.

Enzyme and pathway databases

Reactomei REACT_198830. MHC class II antigen presentation.

Miscellaneous databases

EvolutionaryTracei P09527.
NextBioi 609213.
PROi P09527.

Gene expression databases

Genevestigatori P09527.

Family and domain databases

Gene3Di 3.40.50.300. 1 hit.
InterProi IPR027417. P-loop_NTPase.
IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view ]
Pfami PF00071. Ras. 1 hit.
[Graphical view ]
PRINTSi PR00449. RASTRNSFRMNG.
SMARTi SM00175. RAB. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 1 hit.
TIGRFAMsi TIGR00231. small_GTP. 1 hit.
PROSITEi PS51419. RAB. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "A new member of the ras gene superfamily identified in a rat liver cell line."
    Bucci C., Franzio R., Chiariotti L., Brown A.L., Rechler M.M., Bruni C.B.
    Nucleic Acids Res. 16:9979-9994(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Buffalo.
    Tissue: Liver.
  2. Bruni C.B.
    Submitted (JUN-1994) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO N-TERMINUS.
  3. Zhao H., Gao L., Vaananen K.H.
    Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Bone.
  4. "Fine mapping of radiation susceptibility and gene expression analysis of LEC congenic rat lines."
    Tsuji A.B., Sugyo A., Ogiu T., Sagara M., Kimura T., Ishikawa A., Sudo H., Ohtsuki M., Aburatani H., Imai T., Harada Y.N.
    Genomics 86:271-279(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Fischer 344/DuCrj and LEC/Crj.
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Lung.
  6. "Possible role of direct Rac1-Rab7 interaction in ruffled border formation of osteoclasts."
    Sun Y., Bueki K.G., Ettala O., Vaeaeraeniemi J.P., Vaeaenaenen H.K.
    J. Biol. Chem. 280:32356-32361(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH RAC1.
  7. "The small GTPase Rab7 controls the endosomal trafficking and neuritogenic signaling of the nerve growth factor receptor TrkA."
    Saxena S., Bucci C., Weis J., Kruttgen A.
    J. Neurosci. 25:10930-10940(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, INTERACTION WITH NTRK1/TRKA, TISSUE SPECIFICITY.
  8. "Structures of RabGGTase-substrate/product complexes provide insights into the evolution of protein prenylation."
    Guo Z., Wu Y.W., Das D., Delon C., Cramer J., Yu S., Thuns S., Lupilova N., Waldmann H., Brunsveld L., Goody R.S., Alexandrov K., Blankenfeldt W.
    EMBO J. 27:2444-2456(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBUNIT, INTERACTION WITH CHM.
  9. "Structure of the Rab7:REP-1 complex: insights into the mechanism of Rab prenylation and choroideremia disease."
    Rak A., Pylypenko O., Niculae A., Pyatkov K., Goody R.S., Alexandrov K.
    Cell 117:749-760(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.70 ANGSTROMS) OF 1-185 IN COMPLEX WITH GTP ANALOG AND CHM.

Entry informationi

Entry nameiRAB7A_RAT
AccessioniPrimary (citable) accession number: P09527
Secondary accession number(s): Q4AEF6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: October 1, 1996
Last modified: September 3, 2014
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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