P09527 (RAB7A_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ras-related protein Rab-7a

Alternative name(s):
Ras-related protein BRL-RAS
Ras-related protein p23

Gene names

Name:	Rab7a
Synonyms:	Rab7

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	207 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Key regulator in endo-lysosomal trafficking. Governs early-to-late endosomal maturation, microtubule minus-end as well as plus-end directed endosomal migration and positioning, and endosome-lysosome transport through different protein-protein interaction cascades. Plays a central role, not only in endosomal traffic, but also in many other cellular and physiological events, such as growth-factor-mediated cell signaling, nutrient-transportor mediated nutrient uptake, neurotrophin transport in the axons of neurons and lipid metabolism. Also involved in regulation of some specialized endosomal membrane trafficking, such as maturation of melanosomes, pathogen-induced phagosomes (or vacuoles) and autophagosomes. Plays a role in the maturation and acidification of phagosomes that engulf pathogens, such as S.aureus and Mycobacteria. Plays important roles in microbial pathogen infection and survival, as well as in participating in the life cycle of viruses. Microbial pathogens possess survival strategies governed by RAB7A, sometimes by employing RAB7A function (e.g. Salmonella) and sometimes by excluding RAB7A function (e.g. Mycobacterium). In concert with RAC1, plays a role in regulating the formation of RBs (ruffled borders) in osteoclasts. Controls the endosomal trafficking and neurite outgrowth signaling of NTRK1/TRKA. Regulates the endocytic trafficking of the EGF-EGFR complex by regulating its lysosomal degradation. Ref.6 Ref.7
Subunit structure	Interacts with RILP, PSMA7, RNF115 and FYCO1. Interacts with the PIK3C3/VPS34-PIK3R4 complex. The GTP-bound form interacts with OSBPL1A and RAC1 By similarity. Interacts with NTRK1/TRKA. The GTP-bound form interacts with RAC1. Interacts with CLN3 By similarity. Ref.6 Ref.7
Subcellular location	Late endosome. Lysosome. Cytoplasmic vesicle › phagosome By similarity. Cytoplasmic vesicle › phagosome membrane; Lipid-anchor; Cytoplasmic side By similarity. Melanosome By similarity. Note: Co-localizes with OSBPL1A at the late endosome. Recruited to phagosomes containing S.aureus or Mycobacterium By similarity. Found in the ruffled border (a late endosomal-like compartment in the plasma membrane) of bone-resorbing osteoclasts. Ref.6
Tissue specificity	Expressed in osteoclasts and in neurons. Ref.6 Ref.7
Sequence similarities	Belongs to the small GTPase superfamily. Rab family.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Cytoplasmic vesicle Endosome Lysosome Membrane
Ligand	GTP-binding Nucleotide-binding
PTM	Lipoprotein Methylation Phosphoprotein Prenylation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	bone resorption Inferred from mutant phenotype PubMed 11514537. Source: RGD early endosome to late endosome transport Inferred from sequence or structural similarity. Source: UniProtKB endosome to lysosome transport Inferred from electronic annotation. Source: Compara epidermal growth factor catabolic process Inferred from electronic annotation. Source: Compara phagosome acidification Inferred from sequence or structural similarity. Source: UniProtKB phagosome-lysosome fusion Inferred from sequence or structural similarity. Source: UniProtKB protein targeting to lysosome Inferred from electronic annotation. Source: Compara small GTPase mediated signal transduction Inferred from electronic annotation. Source: InterPro
Cellular_component	Golgi apparatus Inferred from electronic annotation. Source: Compara alveolar lamellar body Inferred from direct assay PubMed 7868367. Source: RGD late endosome Inferred from direct assay PubMed 19337031. Source: RGD lysosome Inferred from electronic annotation. Source: UniProtKB-SubCell melanosome Inferred from electronic annotation. Source: UniProtKB-SubCell phagocytic vesicle Inferred from sequence or structural similarity. Source: UniProtKB phagocytic vesicle membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	GDP binding Inferred from direct assay Ref.6. Source: RGD GTP binding Inferred from direct assay Ref.6. Source: RGD GTPase activity Traceable author statement PubMed 11514537. Source: RGD
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
Chm	P37727	2	EBI-916225,EBI-1039231

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 207

207

Ras-related protein Rab-7a

PRO_0000121124

Regions

Nucleotide binding

15 – 22

8

GTP By similarity

Nucleotide binding

63 – 67

5

GTP By similarity

Nucleotide binding

125 – 128

4

GTP By similarity

Motif

37 – 45

9

Effector region By similarity

Amino acid modifications

Modified residue

72

1

Phosphoserine By similarity

Modified residue

207

1

Cysteine methyl ester By similarity

Lipidation

205

1

S-geranylgeranyl cysteine By similarity

Lipidation

207

1

S-geranylgeranyl cysteine By similarity

Secondary structure

1

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207

Helix Strand Turn

Details...

Beta strand

8 – 14

7

Helix

21 – 30

10

Beta strand

42 – 54

13

Beta strand

56 – 64

9

Helix

68 – 70

3

Helix

76 – 78

3

Beta strand

82 – 89

8

Helix

93 – 97

5

Helix

99 – 110

12

Helix

115 – 117

3

Beta strand

120 – 125

6

Beta strand

129 – 131

3

Helix

136 – 145

10

Beta strand

151 – 153

3

Turn

156 – 159

4

Helix

162 – 185

24

Sequences

	Sequence		Length	Mass (Da)
	P09527 [UniParc]. Last modified October 1, 1996. Version 2. Checksum: A2AF33B02F672971	FASTA	207	23,504
	10 20 30 40 50 60 MTSRKKVLLK VIILGDSGVG KTSLMNQYVN KKFSNQYKAT IGADFLTKEV MVDDRLVTMQ 70 80 90 100 110 120 IWDTAGQERF QSLGVAFYRG ADCCVLVFDV TAPNTFKTLD SWRDEFLIQA SPRDPENFPF 130 140 150 160 170 180 VVLGNKIDLE NRQVATKRAQ AWCYSKNNIP YFETSAKEAI NVEQAFQTIA RNALKQETEV 190 200 ELYNEFPEPI KLDKNERAKA SAESCSC

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A new member of the ras gene superfamily identified in a rat liver cell line." Bucci C., Franzio R., Chiariotti L., Brown A.L., Rechler M.M., Bruni C.B. Nucleic Acids Res. 16:9979-9994(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Buffalo. Tissue: Liver.
[2]	Bruni C.B. Submitted (JUN-1994) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO N-TERMINUS.
[3]	Zhao H., Gao L., Vaananen K.H. Submitted (JUL-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Sprague-Dawley. Tissue: Bone.
[4]	"Fine mapping of radiation susceptibility and gene expression analysis of LEC congenic rat lines." Tsuji A.B., Sugyo A., Ogiu T., Sagara M., Kimura T., Ishikawa A., Sudo H., Ohtsuki M., Aburatani H., Imai T., Harada Y.N. Genomics 86:271-279(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: Fischer 344/DuCrj and LEC/Crj.
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Lung.
[6]	"Possible role of direct Rac1-Rab7 interaction in ruffled border formation of osteoclasts." Sun Y., Bueki K.G., Ettala O., Vaeaeraeniemi J.P., Vaeaenaenen H.K. J. Biol. Chem. 280:32356-32361(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INTERACTION WITH RAC1.
[7]	"The small GTPase Rab7 controls the endosomal trafficking and neuritogenic signaling of the nerve growth factor receptor TrkA." Saxena S., Bucci C., Weis J., Kruttgen A. J. Neurosci. 25:10930-10940(2005) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, INTERACTION WITH NTRK1/TRKA, TISSUE SPECIFICITY.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

X12535 mRNA. Translation: CAA31053.1.
AF286535 mRNA. Translation: AAG00543.1.
AB158427 mRNA. Translation: BAE16999.1.
AB158428 mRNA. Translation: BAE17000.1.
BC072470 mRNA. Translation: AAH72470.1.

IPI

IPI00215564.

PIR

S01934.

RefSeq

NP_076440.1. NM_023950.3.

UniGene

Rn.1425.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1VG0	X-ray	2.20	B	1-207	[»]
1VG1	X-ray	1.90	A	1-185	[»]
1VG8	X-ray	1.70	A/B/C/D	1-207	[»]
1VG9	X-ray	2.50	B/D/F/H	1-185	[»]

ProteinModelPortal

P09527.

SMR

P09527. Positions 7-190.

ModBase

Search...

Protein-protein interaction databases

IntAct

P09527. 2 interactions.

MINT

MINT-1775656.

STRING

10116.ENSRNOP00000016432.

PTM databases

PhosphoSite

P09527.

Proteomic databases

PaxDb

P09527.

PRIDE

P09527.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSRNOT00000016432; ENSRNOP00000016432; ENSRNOG00000012247.

GeneID

29448.

KEGG

rno:29448.

UCSC

RGD:61908. rat.

Organism-specific databases

CTD

7879.

RGD

61908. Rab7a.

Phylogenomic databases

eggNOG

COG1100.

GeneTree

ENSGT00700000104345.

HOGENOM

HOG000233968.

HOVERGEN

HBG009351.

InParanoid

P09527.

KO

K07897.

OMA

SPRDPEH.

OrthoDB

EOG4QFWF4.

Gene expression databases

Genevestigator

P09527.

GermOnline

ENSRNOG00000012247. Rattus norvegicus.

Family and domain databases

InterPro

IPR005225. Small_GTP-bd_dom.
IPR001806. Small_GTPase.
IPR003579. Small_GTPase_Rab_type.
[Graphical view]

Pfam

PF00071. Ras. 1 hit.
[Graphical view]

PRINTS

PR00449. RASTRNSFRMNG.

SMART

SM00175. RAB. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00231. small_GTP. 1 hit.

PROSITE

PS51419. RAB. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P09527.

NextBio

609213.

Entry information

Entry name

RAB7A_RAT

Accession

Primary (citable) accession number: P09527
Secondary accession number(s): Q4AEF6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 121 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program