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Protein

Annexin A4

Gene

ANXA4

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Calcium/phospholipid-binding protein which promotes membrane fusion and is involved in exocytosis.By similarity

GO - Molecular functioni

  • calcium-dependent phospholipid binding Source: UniProtKB
  • calcium-dependent protein binding Source: AgBase
  • calcium ion binding Source: BHF-UCL
  • identical protein binding Source: BHF-UCL
  • NF-kappaB binding Source: BHF-UCL
  • phospholipase inhibitor activity Source: UniProtKB

GO - Biological processi

  • epithelial cell differentiation Source: UniProtKB
  • negative regulation of apoptotic process Source: UniProtKB
  • negative regulation of interleukin-8 secretion Source: BHF-UCL
  • negative regulation of NF-kappaB transcription factor activity Source: BHF-UCL
  • Notch signaling pathway Source: Ensembl
  • regulation of transcription from RNA polymerase II promoter Source: BHF-UCL
  • signal transduction Source: UniProtKB
Complete GO annotation...

Keywords - Ligandi

Calcium, Calcium/phospholipid-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Annexin A4
Alternative name(s):
35-beta calcimedin
Annexin IV
Annexin-4
Carbohydrate-binding protein p33/p41
Chromobindin-4
Endonexin I
Lipocortin IV
P32.5
PP4-X
Placental anticoagulant protein II
Short name:
PAP-II
Protein II
Gene namesi
Name:ANXA4
Synonyms:ANX4
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

HGNCiHGNC:542. ANXA4.

Subcellular locationi

GO - Cellular componenti

  • cell surface Source: BHF-UCL
  • cytoplasm Source: UniProtKB
  • extracellular exosome Source: UniProtKB
  • nuclear membrane Source: BHF-UCL
  • nucleus Source: BHF-UCL
  • perinuclear region of cytoplasm Source: BHF-UCL
  • plasma membrane Source: BHF-UCL
  • vesicle membrane Source: BHF-UCL
Complete GO annotation...

Pathology & Biotechi

Organism-specific databases

DisGeNETi307.
PharmGKBiPA24832.

Polymorphism and mutation databases

BioMutaiANXA4.
DMDMi1703319.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00000674822 – 319Annexin A4Add BLAST318

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei7PhosphothreonineBy similarity1
Modified residuei12PhosphoserineCombined sources1
Cross-linki28Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1)By similarity
Cross-linki28Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei69N6-acetyllysineBy similarity1
Modified residuei100N6-acetyllysineBy similarity1
Modified residuei213N6-acetyllysineCombined sources1
Modified residuei293N6-acetyllysineCombined sources1
Modified residuei300N6-acetyllysineCombined sources1

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP09525.
MaxQBiP09525.
PaxDbiP09525.
PeptideAtlasiP09525.
PRIDEiP09525.

2D gel databases

DOSAC-COBS-2DPAGEP09525.
OGPiP09525.
REPRODUCTION-2DPAGEIPI00793199.
P09525.
SWISS-2DPAGEP09525.

PTM databases

iPTMnetiP09525.
PhosphoSitePlusiP09525.
SwissPalmiP09525.

Expressioni

Gene expression databases

BgeeiENSG00000196975.
CleanExiHS_ANXA4.
ExpressionAtlasiP09525. baseline and differential.
GenevisibleiP09525. HS.

Organism-specific databases

HPAiCAB005076.
CAB017560.
HPA007393.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
TINF2Q9BSI42EBI-2556852,EBI-717399

GO - Molecular functioni

  • calcium-dependent protein binding Source: AgBase
  • identical protein binding Source: BHF-UCL
  • NF-kappaB binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi106804. 18 interactors.
IntActiP09525. 6 interactors.
MINTiMINT-4528752.
STRINGi9606.ENSP00000377833.

Structurei

Secondary structure

1319
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi16 – 26Combined sources11
Beta strandi28 – 31Combined sources4
Helixi34 – 41Combined sources8
Helixi46 – 59Combined sources14
Helixi64 – 71Combined sources8
Helixi74 – 84Combined sources11
Helixi87 – 99Combined sources13
Beta strandi100 – 103Combined sources4
Helixi106 – 115Combined sources10
Helixi118 – 132Combined sources15
Helixi136 – 143Combined sources8
Helixi146 – 155Combined sources10
Turni156 – 158Combined sources3
Helixi168 – 181Combined sources14
Turni182 – 184Combined sources3
Beta strandi185 – 187Combined sources3
Helixi190 – 199Combined sources10
Helixi202 – 216Combined sources15
Helixi220 – 227Combined sources8
Helixi230 – 244Combined sources15
Helixi246 – 258Combined sources13
Beta strandi259 – 262Combined sources4
Helixi265 – 275Combined sources11
Turni276 – 279Combined sources4
Helixi280 – 291Combined sources12
Helixi295 – 302Combined sources8
Helixi305 – 315Combined sources11

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ZOCX-ray2.00A/B1-319[»]
ProteinModelPortaliP09525.
SMRiP09525.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP09525.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati23 – 83Annexin 1Add BLAST61
Repeati95 – 155Annexin 2Add BLAST61
Repeati179 – 239Annexin 3Add BLAST61
Repeati254 – 314Annexin 4Add BLAST61

Domaini

A pair of annexin repeats may form one binding site for calcium and phospholipid.

Sequence similaritiesi

Belongs to the annexin family.Curated
Contains 4 annexin repeats.Curated

Keywords - Domaini

Annexin, Repeat

Phylogenomic databases

eggNOGiKOG0819. Eukaryota.
ENOG410XPUN. LUCA.
HOVERGENiHBG061815.
InParanoidiP09525.
KOiK17093.
OrthoDBiEOG091G0H6H.
PhylomeDBiP09525.
TreeFamiTF105452.

Family and domain databases

Gene3Di1.10.220.10. 4 hits.
InterProiIPR001464. Annexin.
IPR018502. Annexin_repeat.
IPR018252. Annexin_repeat_CS.
IPR002391. AnnexinIV.
[Graphical view]
PANTHERiPTHR10502:SF28. PTHR10502:SF28. 1 hit.
PfamiPF00191. Annexin. 4 hits.
[Graphical view]
PRINTSiPR00196. ANNEXIN.
SMARTiSM00335. ANX. 4 hits.
[Graphical view]
PROSITEiPS00223. ANNEXIN. 4 hits.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P09525-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MATKGGTVKA ASGFNAMEDA QTLRKAMKGL GTDEDAIISV LAYRNTAQRQ
60 70 80 90 100
EIRTAYKSTI GRDLIDDLKS ELSGNFEQVI VGMMTPTVLY DVQELRRAMK
110 120 130 140 150
GAGTDEGCLI EILASRTPEE IRRISQTYQQ QYGRSLEDDI RSDTSFMFQR
160 170 180 190 200
VLVSLSAGGR DEGNYLDDAL VRQDAQDLYE AGEKKWGTDE VKFLTVLCSR
210 220 230 240 250
NRNHLLHVFD EYKRISQKDI EQSIKSETSG SFEDALLAIV KCMRNKSAYF
260 270 280 290 300
AEKLYKSMKG LGTDDNTLIR VMVSRAEIDM LDIRAHFKRL YGKSLYSFIK
310
GDTSGDYRKV LLVLCGGDD
Length:319
Mass (Da):35,883
Last modified:January 23, 2007 - v4
Checksum:i3799FE80A93AB215
GO
Isoform 2 (identifier: P09525-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-82: Missing.

Note: No experimental confirmation available.
Show »
Length:237
Mass (Da):27,063
Checksum:iC512EFA927DBDD7D
GO

Sequence cautioni

The sequence AAC41689 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAH00182 differs from that shown. Reason: Erroneous initiation.Curated
The sequence AAH11659 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAA11227 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti96R → Q in AAC41689 (PubMed:2970257).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_05550085T → M.Corresponds to variant rs2228203dbSNPEnsembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0563961 – 82Missing in isoform 2. 1 PublicationAdd BLAST82

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M19383 mRNA. Translation: AAC41689.1. Different initiation.
M82809 mRNA. Translation: AAA51740.1.
D78152 mRNA. Translation: BAA11227.1. Different initiation.
AK293177 mRNA. Translation: BAG56720.1.
AC019206 Genomic DNA. No translation available.
AC092431 Genomic DNA. No translation available.
AC112787 Genomic DNA. No translation available.
BC000182 mRNA. Translation: AAH00182.1. Different initiation.
BC011659 mRNA. Translation: AAH11659.1. Different initiation.
PIRiA42077.
RefSeqiNP_001144.1. NM_001153.4.
NP_001307627.1. NM_001320698.1.
NP_001307629.1. NM_001320700.1.
NP_001307631.1. NM_001320702.1. [P09525-2]
XP_016859432.1. XM_017003943.1.
XP_016859433.1. XM_017003944.1. [P09525-2]
UniGeneiHs.422986.

Genome annotation databases

EnsembliENST00000394295; ENSP00000377833; ENSG00000196975.
ENST00000536030; ENSP00000441931; ENSG00000196975.
GeneIDi307.
KEGGihsa:307.

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M19383 mRNA. Translation: AAC41689.1. Different initiation.
M82809 mRNA. Translation: AAA51740.1.
D78152 mRNA. Translation: BAA11227.1. Different initiation.
AK293177 mRNA. Translation: BAG56720.1.
AC019206 Genomic DNA. No translation available.
AC092431 Genomic DNA. No translation available.
AC112787 Genomic DNA. No translation available.
BC000182 mRNA. Translation: AAH00182.1. Different initiation.
BC011659 mRNA. Translation: AAH11659.1. Different initiation.
PIRiA42077.
RefSeqiNP_001144.1. NM_001153.4.
NP_001307627.1. NM_001320698.1.
NP_001307629.1. NM_001320700.1.
NP_001307631.1. NM_001320702.1. [P09525-2]
XP_016859432.1. XM_017003943.1.
XP_016859433.1. XM_017003944.1. [P09525-2]
UniGeneiHs.422986.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2ZOCX-ray2.00A/B1-319[»]
ProteinModelPortaliP09525.
SMRiP09525.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi106804. 18 interactors.
IntActiP09525. 6 interactors.
MINTiMINT-4528752.
STRINGi9606.ENSP00000377833.

PTM databases

iPTMnetiP09525.
PhosphoSitePlusiP09525.
SwissPalmiP09525.

Polymorphism and mutation databases

BioMutaiANXA4.
DMDMi1703319.

2D gel databases

DOSAC-COBS-2DPAGEP09525.
OGPiP09525.
REPRODUCTION-2DPAGEIPI00793199.
P09525.
SWISS-2DPAGEP09525.

Proteomic databases

EPDiP09525.
MaxQBiP09525.
PaxDbiP09525.
PeptideAtlasiP09525.
PRIDEiP09525.

Protocols and materials databases

DNASUi307.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000394295; ENSP00000377833; ENSG00000196975.
ENST00000536030; ENSP00000441931; ENSG00000196975.
GeneIDi307.
KEGGihsa:307.

Organism-specific databases

CTDi307.
DisGeNETi307.
GeneCardsiANXA4.
HGNCiHGNC:542. ANXA4.
HPAiCAB005076.
CAB017560.
HPA007393.
MIMi106491. gene.
neXtProtiNX_P09525.
PharmGKBiPA24832.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0819. Eukaryota.
ENOG410XPUN. LUCA.
HOVERGENiHBG061815.
InParanoidiP09525.
KOiK17093.
OrthoDBiEOG091G0H6H.
PhylomeDBiP09525.
TreeFamiTF105452.

Miscellaneous databases

ChiTaRSiANXA4. human.
EvolutionaryTraceiP09525.
GeneWikiiANXA4.
GenomeRNAii307.
PROiP09525.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000196975.
CleanExiHS_ANXA4.
ExpressionAtlasiP09525. baseline and differential.
GenevisibleiP09525. HS.

Family and domain databases

Gene3Di1.10.220.10. 4 hits.
InterProiIPR001464. Annexin.
IPR018502. Annexin_repeat.
IPR018252. Annexin_repeat_CS.
IPR002391. AnnexinIV.
[Graphical view]
PANTHERiPTHR10502:SF28. PTHR10502:SF28. 1 hit.
PfamiPF00191. Annexin. 4 hits.
[Graphical view]
PRINTSiPR00196. ANNEXIN.
SMARTiSM00335. ANX. 4 hits.
[Graphical view]
PROSITEiPS00223. ANNEXIN. 4 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiANXA4_HUMAN
AccessioniPrimary (citable) accession number: P09525
Secondary accession number(s): B4DDF9, Q96F33, Q9BWK1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: January 23, 2007
Last modified: November 2, 2016
This is version 181 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Miscellaneous

Seems to bind one calcium ion with high affinity.

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  6. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.