P09514 (MCAPS_TYYVF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Minor capsid protein P3-RTD

Alternative name(s):
P74
Readthrough protein
Short name=RT protein

Gene names

ORF Names:

ORF3/ORF5

Organism

Turnip yellows virus (isolate FL-1) (TuYV) (BWYV-FL1) [Complete proteome]

Taxonomic identifier

12043 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA positive-strand viruses, no DNA stage › Luteoviridae › Polerovirus › Beet western yellows virus

Virus host

Beta vulgaris (Sugar beet) [TaxID: 161934]
Brassica napus var. napobrassica [TaxID: 3709]
Brassica napus var. napus [TaxID: 138011]
Brassica nigra (Black mustard) (Sinapis nigra) [TaxID: 3710]
Brassica oleracea var. botrytis (Cauliflower) [TaxID: 3715]
Brassica oleracea var. capitata (Cabbage) [TaxID: 3716]
Brassica rapa (Turnip) [TaxID: 51350]
Capsicum annuum (Bell pepper) [TaxID: 4072]
Cicer arietinum (Chickpea) (Garbanzo) [TaxID: 3827]
Citrullus lanatus (Watermelon) (Citrullus vulgaris) [TaxID: 3654]
Crambe hispanica subsp. abyssinica (Abyssinian kale) (Crambe abyssinica) [TaxID: 3721]
Cucumis sativus (Cucumber) [TaxID: 3659]
Cucurbita pepo (Vegetable marrow) (Summer squash) [TaxID: 3663]
Glycine max (Soybean) (Glycine hispida) [TaxID: 3847]
Helianthus annuus (Common sunflower) [TaxID: 4232]
Lactuca sativa (Garden lettuce) [TaxID: 4236]
Phlox drummondii (Annual phlox) [TaxID: 103529]
Pisum sativum (Garden pea) [TaxID: 3888]
Raphanus sativus (Radish) [TaxID: 3726]
Solanum lycopersicum (Tomato) (Lycopersicon esculentum) [TaxID: 4081]
Spinacia oleracea (Spinach) [TaxID: 3562]
Trifolium subterraneum (Subterranean clover) [TaxID: 3900]
Vicia faba (Broad bean) (Faba vulgaris) [TaxID: 3906]

Protein attributes

Sequence length	670 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Minor component of the viral capsid involved in aphid transmission. The RTD domain of the protein is exposed on the surface of the particle and determines the vector specificity and intestinal tropism in the aphid. This domain also determines the limitation to host phloem By similarity. Ref.2
Subcellular location	Minor capsid protein P3-RTD: Virion Potential.
Miscellaneous	This protein is translated as a fusion protein by episodic readthrough of the major coat protein termination codon. It is composed of the major capsid protein fused to a long C-terminal extension called the readthrough domain (RTD). Readthrough of the terminator codon TAG occurs between the codons for 202-Lys and 204-Val. The N-terminal region like those of many plant virus capsid proteins is highly basic. It has been suggested that these regions may be involved in protein-RNA interaction.
Sequence similarities	Belongs to the luteoviruses readthrough protein family.
Sequence caution	The sequence CAA31467.2 differs from that shown. Reason: Readthrough of an in-frame TAG stop codon in position 203 translated as Tyr.

Ontologies

Keywords
Cellular component	Virion
Coding sequence diversity	RNA suppression of termination
Molecular function	Capsid protein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular_component	viral capsid Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 670	670	Minor capsid protein P3-RTD		PRO_0000222426
Regions
	Region	204 – 670	467	Readthrough domain (RTD)
	Compositional bias	7 – 59	53	Arg-rich
	Compositional bias	29 – 32	4	Poly-Val
Experimental info
	Mutagenesis	227	1	R → A: Complete loss of aphid transmission. Ref.3
	Mutagenesis	262 – 263	2	ED → A: Complete loss of aphid transmission.
	Mutagenesis	316 – 317	2	KD → A: No effect on aphid transmission.
	Mutagenesis	403 – 404	2	KY → A: Complete loss of aphid transmission.
	Mutagenesis	428 – 429	2	DE → A: Almost complete loss of aphid transmission.

Sequences

	Sequence		Length	Mass (Da)
	P09514 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 4301B21EE7B4BE9F	FASTA	670	74,016
	10 20 30 40 50 60 MNTVVGRRII NGRRRPRRQT RRAQRPQPVV VVQTSRATQR RPRRRRRGNN RTGRTVPTRG 70 80 90 100 110 120 AGSSETFVFS KDNLAGSSSG AITFGPSLSD CPAFSNGMLK AYHEYKISMV ILEFVSEASS 130 140 150 160 170 180 QNSGSIAYEL DPHCKLNSLS STINKFGITK PGKRAFTASY INGTEWHDVA EDQFRILYKG 190 200 210 220 230 240 NGSSSIAGSF RITIKCQFHN PKYVDEEPGP SPGPSPSPQP TPQKKYRFIV YTGVPVTRIM 250 260 270 280 290 300 AQSTDDAISL YDMPSQRFRY IEDENMNWTN LDSRWYSQNS LKAIPMIIVP VPQGEWTVEI 310 320 330 340 350 360 SMEGYQPTSS TTDPNKDKQD GLIAYNDDLS EGWNVGIYNN VEITNNKADN TLKYGHPDME 370 380 390 400 410 420 LNGCHFNQGQ CLERDGDLTC HIKTTGDNAS FFVVGPAVQK QSKYNYAVSY GAWTDRMMEI 430 440 450 460 470 480 GMIAIALDEQ GSSGSVKTER PKRVGHSMAV STWETIKLPE KGNSEGYETS QRQDSKTPPT 490 500 510 520 530 540 ASGGSDTLDV EEGGLPLPVE EEIPDFVGDN PWSDLSTKNS QEEEAMSSES GLRPQLKPPG 550 560 570 580 590 600 LPKPQPIRTI RNFDPTPDLV EAWRPDVNPG YSKADVAAAT IIAGGSIKDG RSMIDKRNKA 610 620 630 640 650 660 VLDGRKSWGS SLASSLTGGT LKASAKSEKL AKLTTSERAR YERIKRQQGS TRASEFLESL 670 LAGEDPDSRF

References

[1]	"Nucleotide sequence of beet western yellows virus RNA." Veidt I., Lot H., Leiser M., Scheidecker D., Guilley H., Richards K.E., Jonard G. Nucleic Acids Res. 16:9917-9932(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	"Aphid transmission of beet western yellows luteovirus requires the minor capsid read-through protein P74." Brault V., van den Heuvel J.F., Verbeek M., Ziegler-Graff V., Reutenauer A., Herrbach E., Garaud J.-C., Guilley H., Richards K.E., Jonard G. EMBO J. 14:650-659(1995) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[3]	"Effects of point mutations in the readthrough domain of the beet western yellows virus minor capsid protein on virus accumulation in planta and on transmission by aphids." Brault V., Mutterer J., Scheidecker D., Simonis M.T., Herrbach E., Richards K.E., Ziegler-Graff V. J. Virol. 74:1140-1148(2000) [PubMed] [Europe PMC] [Abstract] Cited for: MUTAGENESIS OF ARG-227; 262-GLU-ASP-263; 316-LYS-ASP-317; 403-LYS-TYR-404 AND 428-ASP-GLU-429.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X13063 Genomic RNA. Translation: CAA31467.2. Sequence problems.
PIR	S01943.
RefSeq	NP_620487.1. NC_003743.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	940480.
Family and domain databases
InterPro	IPR001517. Luteo_coat. IPR002929. PLrV_ORF5. [Graphical view]
Pfam	PF00894. Luteo_coat. 1 hit. PF01690. PLRV_ORF5. 1 hit. [Graphical view]
PRINTS	PR00910. LVIRUSORF6.
ProtoNet	Search...

Entry information

Entry name

MCAPS_TYYVF

Accession

Primary (citable) accession number: P09514

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	January 23, 2007
Last modified:	April 3, 2013
This is version 57 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program