P09507 (RDRP_TYYVF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Protein P1-P2

Cleaved into the following 2 chains:

1. Serine protease
   EC=3.4.21.-
2. RNA-directed RNA polymerase
   EC=2.7.7.48

Gene names

ORF Names:

ORF1/ORF2

Organism

Turnip yellows virus (isolate FL-1) (TuYV) (BWYV-FL1) [Complete proteome]

Taxonomic identifier

12043 [NCBI]

Taxonomic lineage

Viruses › ssRNA viruses › ssRNA positive-strand viruses, no DNA stage › Luteoviridae › Polerovirus › Beet western yellows virus

Virus host

Beta vulgaris (Sugar beet) [TaxID: 161934]
Brassica napus var. napobrassica [TaxID: 3709]
Brassica napus var. napus [TaxID: 138011]
Brassica nigra (Black mustard) (Sinapis nigra) [TaxID: 3710]
Brassica oleracea var. botrytis (Cauliflower) [TaxID: 3715]
Brassica oleracea var. capitata (Cabbage) [TaxID: 3716]
Brassica rapa (Turnip) [TaxID: 51350]
Capsicum annuum (Bell pepper) [TaxID: 4072]
Cicer arietinum (Chickpea) (Garbanzo) [TaxID: 3827]
Citrullus lanatus (Watermelon) (Citrullus vulgaris) [TaxID: 3654]
Crambe hispanica subsp. abyssinica (Abyssinian kale) (Crambe abyssinica) [TaxID: 3721]
Cucumis sativus (Cucumber) [TaxID: 3659]
Cucurbita pepo (Vegetable marrow) (Summer squash) [TaxID: 3663]
Glycine max (Soybean) (Glycine hispida) [TaxID: 3847]
Helianthus annuus (Common sunflower) [TaxID: 4232]
Lactuca sativa (Garden lettuce) [TaxID: 4236]
Phlox drummondii (Annual phlox) [TaxID: 103529]
Pisum sativum (Garden pea) [TaxID: 3888]
Raphanus sativus (Radish) [TaxID: 3726]
Solanum lycopersicum (Tomato) (Lycopersicon esculentum) [TaxID: 4081]
Spinacia oleracea (Spinach) [TaxID: 3562]
Trifolium subterraneum (Subterranean clover) [TaxID: 3900]
Vicia faba (Broad bean) (Faba vulgaris) [TaxID: 3906]

Protein attributes

Sequence length	1035 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Predicted

General annotation (Comments)

Function	Precursor from which the RNA-dependent RNA polymerase (RdRp) is probably released. RNA-dependent RNA polymerase plays an essential role in virus replication Potential.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1).
Subcellular location	Protein P1-P2: Membrane; Multi-pass membrane protein Potential.
Post-translational modification	Specific enzymatic cleavages in vivo yield mature proteins. The protease probably cleaves itself and releases the RdRp Potential. Cleavages have been shown in the P1 protein, but since the N-terminus containing the serine protease is shared between P1 and P1-P2, cleavages should also occur within the P1-P2 protein.
Sequence similarities	Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	Viral RNA replication
Cellular component	Membrane
Coding sequence diversity	Ribosomal frameshifting
Domain	Signal Transmembrane Transmembrane helix
Ligand	Nucleotide-binding
Molecular function	Hydrolase Nucleotidyltransferase Protease RNA-directed RNA polymerase Serine protease Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro viral genome replication Inferred from electronic annotation. Source: InterPro
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	RNA binding Inferred from electronic annotation. Source: InterPro RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by ribosomal frameshifting. [Align] [Select]

Isoform RNA-directed RNA polymerase (identifier: P09507-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: Produced by -1 ribosomal frameshifting between codons 461 and 462.

Isoform Protein P1 (identifier: P09506-1)

The sequence of this isoform can be found in the external entry P09506.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: Produced by conventional translation.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Potential
	Chain	23 – 1035	1013	Protein P1-P2		PRO_0000222398
	Chain	204 – 400	197	Serine protease Potential		PRO_0000390903
	Chain	401 – 1035	635	RNA-directed RNA polymerase Potential		PRO_0000390904
Regions
	Transmembrane	115 – 135	21	Helical; Potential
	Transmembrane	137 – 157	21	Helical; Potential
	Transmembrane	167 – 187	21	Helical; Potential
	Domain	829 – 944	116	RdRp catalytic
	Compositional bias	818 – 821	4	Poly-Thr
Sites
	Active site	254	1	By similarity
	Active site	289	1	By similarity
	Active site	357	1	By similarity
	Site	203 – 204	2	Cleavage; by viral serine protease Potential
	Site	400 – 401	2	Cleavage; by viral serine protease By similarity

Sequences

	Sequence		Length	Mass (Da)
	Isoform RNA-directed RNA polymerase [UniParc]. Last modified June 20, 2002. Version 2. Checksum: 54642FEC88E6F66F	FASTA	1,035	115,871
	10 20 30 40 50 60 MYSKLMFFFA LCSISFLFTS EAASTMLLES SYLPLNQSYA PGFLYKRDML PPPLQAVLTY 70 80 90 100 110 120 TCPEPRPLAE ESYNDLLRAI SQKSSSDFQN AYSLALSFSS DFYQHGLKTL KDVSFLAVEK 130 140 150 160 170 180 FLWGLTRLWS SLILASFSAL WWLVSNFTTP VFCLALLYTV TKYMVKTVSF LFGGLPIWII 190 200 210 220 230 240 SIAFSLLKKS FSALRSTPKC LYEKAIDGFK SFTIPQSPPK SCVIPITHAS GNHAGYASCI 250 260 270 280 290 300 KLYNGENALM TATHVLRDCP NAVAVSAKGL KTRIPLAEFK TIAKSDKGDV TLLRGPPNWE 310 320 330 340 350 360 GLLGCKAANV ITAANLAKCK ASIYSFDRDG WVSSYAEIVG SEGTDVMVLS HTEGGHSGSP 370 380 390 400 410 420 YFNGKTILGV HSGASATGNY NLMAPIPSLP GLTSPTYVFE TTAPQGRVFA QEDIAEIEGL 430 440 450 460 470 480 YAQVMKRVQQ AEDFKPKTGK YWGDMEDDED IFFESKEDLS GKRSARHRPR NKRRRQLHPK 490 500 510 520 530 540 DKQRRWERDD GENNLISSGK DKSREHREES DRGDLRESDE NSEIPPQKSP KETAGEFERY 550 560 570 580 590 600 FSSLYNWEVP TSPREVPGFR HCGKLPQYYH PKQKEESSWG KTLVGNHPAL GEKTSGFGWP 610 620 630 640 650 660 KFGPEAELKS LRLQASRWLE RAQSAEIPSD AERERVIQKT ADVYHPCQTN GPAATRGGTL 670 680 690 700 710 720 TWNNFMIDFK QAVFSLEFDA GIELPYIAYG KPTHRGWVED QKLLPILAQL TFFRLQKMLE 730 740 750 760 770 780 VNFEDMGPEE LVRNGLCDPI RLFVKGEPHK QAKLDEGRYR LIMSVSLVDQ LVARVLFQNQ 790 800 810 820 830 840 NKREIALWRA IPSKPGFGLS TDEQVLDFVE SLARQVGTTT TEVVANWKNY LTPTDCSGFD 850 860 870 880 890 900 WSVADWMLHD DMIVRNRLTI DLNPATERLR SCWLRCISNS VLCLSDGTLL AQIHPGVQKS 910 920 930 940 950 960 GSYNTSSSNS RIRVMAAFHT GAIWAMAMGD DALESNPADL AAYKKLGFKV EVSGQLEFCS 970 980 990 1000 1010 1020 HIFRAPDLAL PVNENKMIYK LIYGYNPGSG NAEVVSNYLA ACFSVLNELR HDPASVELLY 1030 SWLVDPVLPQ KIPGE
	Isoform Protein P1 [UniParc]. See P09506.		–	–

References

[1]	"Nucleotide sequence of beet western yellows virus RNA." Veidt I., Lot H., Leiser M., Scheidecker D., Guilley H., Richards K.E., Jonard G. Nucleic Acids Res. 16:9917-9932(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	Veidt I., Lot H., Leiser M., Scheidecker D., Guilley H., Richards K.E., Jonard G. Submitted (APR-2002) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X13063 Genomic RNA. Translation: CAA31464.2.
PIR	RRVQFL. S01940.
RefSeq	NP_620485.2. NC_003743.1.
3D structure databases
ProteinModelPortal	P09507.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	940481.
Family and domain databases
InterPro	IPR000382. Peptidase_S39B_luteovirus. IPR001795. RNA-dir_pol_luteovirus. IPR007094. RNA-dir_pol_PSvirus. IPR009003. Trypsin-like_Pept_dom. [Graphical view]
Pfam	PF02122. Peptidase_S39. 1 hit. PF02123. RdRP_4. 1 hit. [Graphical view]
PRINTS	PR00914. LVIRUSRNAPOL.
SUPFAM	SSF50494. Pept_Ser_Cys. 1 hit.
PROSITE	PS50507. RDRP_SSRNA_POS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RDRP_TYYVF

Accession

Primary (citable) accession number: P09507

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	June 20, 2002
Last modified:	April 3, 2013
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program