ID   RDRP_BYDVP              Reviewed;         867 AA.
AC   P09505; P19648;
DT   01-JUL-1989, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1991, sequence version 2.
DT   27-MAR-2024, entry version 99.
DE   RecName: Full=Putative RNA-directed RNA polymerase;
DE            EC=2.7.7.48;
GN   ORFNames=ORF1/ORF2;
OS   Barley yellow dwarf virus (isolate PAV) (BYDV).
OC   Viruses; Riboviria; Orthornavirae; Kitrinoviricota; Tolucaviricetes;
OC   Tolivirales; Tombusviridae; Regressovirinae; Luteovirus;
OC   Luteovirus pavhordei.
OX   NCBI_TaxID=2169986;
OH   NCBI_TaxID=146531; Avena byzantina.
OH   NCBI_TaxID=4498; Avena sativa (Oat).
OH   NCBI_TaxID=4513; Hordeum vulgare (Barley).
OH   NCBI_TaxID=4521; Lolium multiflorum (Italian ryegrass) (Lolium perenne subsp. multiflorum).
OH   NCBI_TaxID=4522; Lolium perenne (Perennial ryegrass).
OH   NCBI_TaxID=4530; Oryza sativa (Rice).
OH   NCBI_TaxID=4550; Secale cereale (Rye).
OH   NCBI_TaxID=4565; Triticum aestivum (Wheat).
OH   NCBI_TaxID=4577; Zea mays (Maize).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC RNA].
RX   PubMed=3399386; DOI=10.1093/nar/16.13.6097;
RA   Miller W.A., Waterhouse P.M., Gerlach W.L.;
RT   "Sequence and organization of barley yellow dwarf virus genomic RNA.";
RL   Nucleic Acids Res. 16:6097-6111(1988).
RN   [2]
RP   RIBOSOMAL FRAMESHIFT.
RX   PubMed=12149516; DOI=10.1073/pnas.162223099;
RA   Barry J.K., Miller W.A.;
RT   "A -1 ribosomal frameshift element that requires base pairing across four
RT   kilobases suggests a mechanism of regulating ribosome and replicase traffic
RT   on a viral RNA.";
RL   Proc. Natl. Acad. Sci. U.S.A. 99:11133-11138(2002).
CC   -!- FUNCTION: Probable polymerase.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a ribonucleoside 5'-triphosphate + RNA(n) = diphosphate +
CC         RNA(n+1); Xref=Rhea:RHEA:21248, Rhea:RHEA-COMP:14527, Rhea:RHEA-
CC         COMP:17342, ChEBI:CHEBI:33019, ChEBI:CHEBI:61557, ChEBI:CHEBI:140395;
CC         EC=2.7.7.48; Evidence={ECO:0000255|PROSITE-ProRule:PRU00539};
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Ribosomal frameshifting; Named isoforms=2;
CC       Name=Putative RNA-directed RNA polymerase;
CC         IsoId=P09505-1; Sequence=Displayed;
CC       Name=39 kDa protein;
CC         IsoId=P09505-2; Sequence=VSP_031895;
CC   -!- MISCELLANEOUS: [Isoform Putative RNA-directed RNA polymerase]: Produced
CC       by -1 ribosomal frameshifting between codons 339 and 340.
CC   -!- MISCELLANEOUS: [Isoform 39 kDa protein]: Produced by conventional
CC       translation. {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the luteoviruses RNA polymerase family.
CC       {ECO:0000305}.
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DR   EMBL; X07653; CAA30498.1; ALT_SEQ; Genomic_RNA.
DR   PIR; A59303; A59303.
DR   Proteomes; UP000006722; Genome.
DR   GO; GO:0000166; F:nucleotide binding; IEA:UniProtKB-KW.
DR   GO; GO:0003723; F:RNA binding; IEA:InterPro.
DR   GO; GO:0003968; F:RNA-dependent RNA polymerase activity; IEA:UniProtKB-KW.
DR   GO; GO:0039694; P:viral RNA genome replication; IEA:InterPro.
DR   CDD; cd23233; Luteovirus_RdRp; 1.
DR   Gene3D; 3.30.70.270; -; 1.
DR   InterPro; IPR043502; DNA/RNA_pol_sf.
DR   InterPro; IPR013674; Luteo_Rpol_P1-P2.
DR   InterPro; IPR043128; Rev_trsase/Diguanyl_cyclase.
DR   InterPro; IPR007094; RNA-dir_pol_PSvirus.
DR   InterPro; IPR002166; RNA_pol_HCV.
DR   Pfam; PF08467; Luteo_P1-P2; 1.
DR   Pfam; PF00998; RdRP_3; 1.
DR   SUPFAM; SSF56672; DNA/RNA polymerases; 1.
DR   PROSITE; PS50507; RDRP_SSRNA_POS; 1.
PE   3: Inferred from homology;
KW   Nucleotide-binding; Nucleotidyltransferase; Reference proteome;
KW   Ribosomal frameshifting; RNA-directed RNA polymerase; Transferase;
KW   Viral RNA replication.
FT   CHAIN           1..867
FT                   /note="Putative RNA-directed RNA polymerase"
FT                   /id="PRO_0000039186"
FT   DOMAIN          561..676
FT                   /note="RdRp catalytic"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00539"
FT   VAR_SEQ         340..867
FT                   /note="Missing (in isoform 39 kDa protein)"
FT                   /evidence="ECO:0000305"
FT                   /id="VSP_031895"
SQ   SEQUENCE   867 AA;  98662 MW;  12E2C7AD52EE593B CRC64;
     MFFEILIGAS AKAVKDFISH CYSRLKSIYY SFKRWLMEIS GQFKAHDAFV NMCFGHMADI
     EDFEAELAEE FAEREDEVEE ARSLLKLLVA QKSKSGVTEA WTDFFTKSRG GVYAPLSCEP
     TRQELEVKSE KLERLLEEQH QFEVRAAKKY IKEKGRGFIN CWNDLRSRLR LVKDVKDEAK
     DNARAAAKIG AEMFAPVDVQ DLYSFTEVKK VETGLMKEVV KEKNGEEEKH LEPIMEEVRS
     IKDTAEARDA ASTWITETVK LKNATLNADE LSLATIARYV ENVGDKFKLD IASKTYLKQV
     ASMSVPIPTN KDIKLKMVLQ SPEARARRER MDVLDSVGFL EGLCTASGFE SPFPILGLPE
     IAVTDGARLR KVSSNIRYLS QTHLGLVYKA PNASLHNALV AVERRVFTVG KGDKAIYPPR
     PEHDIFTDTM DYFQKSIIEE VGYCKTYPAQ LLANSYSAGK RAMYHKAIAS LKTVPYHQKD
     ANVQAFLKKE KHWMTKDIAP RLICPRSKRY NIILGTRLKF NEKKIMHAID SVFGSPTVLS
     GYDNFKQGRI IAKKWQKFAC PVAIGVDASR FDQHVSEQAL KWEHGIYNGI FGDSEMALAL
     EHQITNNIKM FVEDKMLRFK VRGHRMSGDI NTSMGNKLIM CGMMHAYLKK LGVEAELCNN
     GDDCVIITDR ANEKLFDGMY DHFLQYGFNM VTEKPVYELE QLEFCQSKPV SINGKYRMVR
     RPDSIGKDST TLLSMLNQSD VKSYMSAVAQ CGLVLNAGVP ILESFYKCLY RSSGYKKVSE
     EFIKNVISYG TDERLQGRRT YNETPITNHS RMSYWESFGV DPKIQQIVER YYDGLTVSAQ
     LQSVKVTTPH LQSILLSIPE NHSQNEY
//