Reviewed, UniProtKB/Swiss-Prot P09503 (DYR_SHV21)

Names and origin

Protein names

Recommended name:
    Viral dihydrofolate reductase
      Short name=vDHFR
    EC=1.5.1.3

Gene names

Name:	DHFR
Synonyms:	2

Organism

Saimiriine herpesvirus 2 (strain 11) (SaHV-2) (Herpesvirus saimiri) [Complete proteome]

Taxonomic identifier

10383 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Herpesvirales › Herpesviridae › Gammaherpesvirinae › Rhadinovirus

Virus host

Saimiri sciureus (Common squirrel monkey) [TaxID: 9521]

Protein attributes

Sequence length	187 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH.
Pathway	Cofactor biosynthesis; tetrahydrofolate biosynthesis; tetrahydrofolate from dihydrofolate: step 1/1.
Miscellaneous	The reaction catalyzed by this enzyme represents an essential step for de novo glycine and purine synthesis, DNA precursor synthesis, and for the conversion of dUMP to dTMP.
Sequence similarities	Belongs to the dihydrofolate reductase family. Contains 1 DHFR (dihydrofolate reductase) domain.

Ontologies

Keywords
Biological process	One-carbon metabolism
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine biosynthetic process Inferred from electronic annotation. Source: InterPro nucleotide biosynthetic process Inferred from electronic annotation. Source: InterPro one-carbon compound metabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	dihydrofolate reductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 187	187	Viral dihydrofolate reductase		PRO_0000186380
Regions
	Domain	4 – 185	182	DHFR

Sequences

	Sequence		Length	Mass (Da)
	P09503-1 [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 46666EAA3BEA5140	FASTA	187	21,719
	10 20 30 40 50 60 MVQALNCIVA VAQNMGIGKQ GNLPWPRLMN DFKHFQRMTT TSSVPDKQNL VIMGKKTWFS 70 80 90 100 110 120 IPEKNRPLKG RINVVLSKEL KELPHRAHFL AKSLDDALKL TEQPELANKV DMVWIIGGSS 130 140 150 160 170 180 VYKEAMSYPC DLKLFVTRIM QDFECDTFFP EFDLEKYKLL IEYPSVLSNV QEEKSIKYKF EVYEKNH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A gene for dihydrofolate reductase in a herpesvirus." Trimble J.J., Murthy S.C.S., Bakker A., Grassmann R., Desrosiers R.C. Science 239:1145-1147(1988) [PubMed: 2830673] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Deletion mutants of herpesvirus saimiri define an open reading frame necessary for transformation." Murthy S.C.S., Trimble J.J., Desrosiers R.C. J. Virol. 63:3307-3314(1989) [PubMed: 2545905] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Primary structure of the herpesvirus saimiri genome." Albrecht J.-C., Nicholas J., Biller D., Cameron K.R., Biesinger B., Newman C., Wittmann S., Craxton M.A., Coleman H., Fleckenstein B., Honess R.W. J. Virol. 66:5047-5058(1992) [PubMed: 1321287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X64346 Genomic DNA. Translation: CAA45624.1. M28071 Genomic DNA. Translation: AAA58726.1. M19237 Genomic DNA. Translation: AAA46154.1.
RefSeq	NP_040203.1.
3D structure databases
HSSP	HSSP built from PDB template 1DHF based on UniProtKB P00374.
SMR	P09503. Positions 2-186.
ModBase	Search...
Genome annotation databases
GeneID	1488256.
Family and domain databases
InterPro	IPR001796. DHFR_reg. [Graphical view]
Pfam	PF00186. DHFR_1. 1 hit. [Graphical view]
PRINTS	PR00070. DHFR.
PROSITE	PS00075. DHFR_1. 1 hit. PS51330. DHFR_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DYR_SHV21

Accession

Primary (citable) accession number: P09503

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	November 25, 2008
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)