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P09503 (DYR_SHV21) Reviewed, UniProtKB/Swiss-Prot

Last modified October 16, 2013. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Viral dihydrofolate reductase

Short name=vDHFR
EC=1.5.1.3
Gene names
Name:DHFR
Synonyms:2
OrganismSaimiriine herpesvirus 2 (strain 11) (SaHV-2) (Herpesvirus saimiri) [Reference proteome]
Taxonomic identifier10383 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeRhadinovirus
Virus hostSaimiri sciureus (Common squirrel monkey) [TaxID: 9521]

Protein attributes

Sequence length187 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis By similarity.

Catalytic activity

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.

Pathway

Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate: step 1/1.

Sequence similarities

Belongs to the dihydrofolate reductase family.

Contains 1 DHFR (dihydrofolate reductase) domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 187187Viral dihydrofolate reductase
PRO_0000186380

Regions

Domain4 – 185182DHFR
Nucleotide binding16 – 227NADP By similarity
Nucleotide binding55 – 573NADP By similarity
Nucleotide binding77 – 793NADP By similarity
Nucleotide binding117 – 1248NADP By similarity
Region31 – 366Substrate binding By similarity

Sites

Binding site101NADP; via amide nitrogen and carbonyl oxygen By similarity
Binding site711Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
P09503 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 46666EAA3BEA5140

FASTA18721,719
        10         20         30         40         50         60 
MVQALNCIVA VAQNMGIGKQ GNLPWPRLMN DFKHFQRMTT TSSVPDKQNL VIMGKKTWFS 

        70         80         90        100        110        120 
IPEKNRPLKG RINVVLSKEL KELPHRAHFL AKSLDDALKL TEQPELANKV DMVWIIGGSS 

       130        140        150        160        170        180 
VYKEAMSYPC DLKLFVTRIM QDFECDTFFP EFDLEKYKLL IEYPSVLSNV QEEKSIKYKF 


EVYEKNH 

« Hide

References

« Hide 'large scale' references
[1]"A gene for dihydrofolate reductase in a herpesvirus."
Trimble J.J., Murthy S.C.S., Bakker A., Grassmann R., Desrosiers R.C.
Science 239:1145-1147(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Deletion mutants of herpesvirus saimiri define an open reading frame necessary for transformation."
Murthy S.C.S., Trimble J.J., Desrosiers R.C.
J. Virol. 63:3307-3314(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]"Primary structure of the herpesvirus saimiri genome."
Albrecht J.-C., Nicholas J., Biller D., Cameron K.R., Biesinger B., Newman C., Wittmann S., Craxton M.A., Coleman H., Fleckenstein B., Honess R.W.
J. Virol. 66:5047-5058(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X64346 Genomic DNA. Translation: CAA45624.1.
M28071 Genomic DNA. Translation: AAA58726.1.
M19237 Genomic DNA. Translation: AAA46154.1.
RefSeqNP_040203.1. NC_001350.1.

3D structure databases

ProteinModelPortalP09503.
SMRP09503. Positions 2-186.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID1488256.

Phylogenomic databases

ProtClustDBCLSP2509646.

Enzyme and pathway databases

UniPathwayUPA00077; UER00158.

Family and domain databases

Gene3D3.40.430.10. 1 hit.
InterProIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]
PfamPF00186. DHFR_1. 1 hit.
[Graphical view]
PRINTSPR00070. DHFR.
SUPFAMSSF53597. SSF53597. 1 hit.
PROSITEPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDYR_SHV21
AccessionPrimary (citable) accession number: P09503
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: October 16, 2013
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways