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Protein

Viral dihydrofolate reductase

Gene

DHFR

Organism
Saimiriine herpesvirus 2 (strain 11) (SaHV-2) (Herpesvirus saimiri)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis (By similarity).By similarity

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.PROSITE-ProRule annotation

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate.
Proteins known to be involved in this subpathway in this organism are:
  1. Viral dihydrofolate reductase (DHFR)
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei10NADP; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei71SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi16 – 22NADPBy similarity7
Nucleotide bindingi55 – 57NADPBy similarity3
Nucleotide bindingi77 – 79NADPBy similarity3
Nucleotide bindingi117 – 124NADPBy similarity8

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

One-carbon metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158.

Names & Taxonomyi

Protein namesi
Recommended name:
Viral dihydrofolate reductase (EC:1.5.1.3)
Short name:
vDHFR
Gene namesi
Name:DHFR
Synonyms:2
OrganismiSaimiriine herpesvirus 2 (strain 11) (SaHV-2) (Herpesvirus saimiri)
Taxonomic identifieri10383 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeRhadinovirus
Virus hostiSaimiri sciureus (Common squirrel monkey) [TaxID: 9521]
Proteomesi
  • UP000000587 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001863801 – 187Viral dihydrofolate reductaseAdd BLAST187

Proteomic databases

PRIDEiP09503.

Structurei

3D structure databases

ProteinModelPortaliP09503.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 185DHFRPROSITE-ProRule annotationAdd BLAST182

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni31 – 36Substrate bindingBy similarity6

Sequence similaritiesi

Belongs to the dihydrofolate reductase family.Curated
Contains 1 DHFR (dihydrofolate reductase) domain.PROSITE-ProRule annotation

Phylogenomic databases

KOiK00287.

Family and domain databases

CDDicd00209. DHFR. 1 hit.
Gene3Di3.40.430.10. 1 hit.
InterProiIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]
PfamiPF00186. DHFR_1. 1 hit.
[Graphical view]
PRINTSiPR00070. DHFR.
SUPFAMiSSF53597. SSF53597. 1 hit.
PROSITEiPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P09503-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVQALNCIVA VAQNMGIGKQ GNLPWPRLMN DFKHFQRMTT TSSVPDKQNL
60 70 80 90 100
VIMGKKTWFS IPEKNRPLKG RINVVLSKEL KELPHRAHFL AKSLDDALKL
110 120 130 140 150
TEQPELANKV DMVWIIGGSS VYKEAMSYPC DLKLFVTRIM QDFECDTFFP
160 170 180
EFDLEKYKLL IEYPSVLSNV QEEKSIKYKF EVYEKNH
Length:187
Mass (Da):21,719
Last modified:July 1, 1989 - v1
Checksum:i46666EAA3BEA5140
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64346 Genomic DNA. Translation: CAA45624.1.
M28071 Genomic DNA. Translation: AAA58726.1.
M19237 Genomic DNA. Translation: AAA46154.1.
RefSeqiNP_040203.1. NC_001350.1.

Genome annotation databases

GeneIDi1488256.
KEGGivg:1488256.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64346 Genomic DNA. Translation: CAA45624.1.
M28071 Genomic DNA. Translation: AAA58726.1.
M19237 Genomic DNA. Translation: AAA46154.1.
RefSeqiNP_040203.1. NC_001350.1.

3D structure databases

ProteinModelPortaliP09503.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP09503.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi1488256.
KEGGivg:1488256.

Phylogenomic databases

KOiK00287.

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158.

Family and domain databases

CDDicd00209. DHFR. 1 hit.
Gene3Di3.40.430.10. 1 hit.
InterProiIPR012259. DHFR.
IPR024072. DHFR-like_dom.
IPR017925. DHFR_CS.
IPR001796. DHFR_dom.
[Graphical view]
PfamiPF00186. DHFR_1. 1 hit.
[Graphical view]
PRINTSiPR00070. DHFR.
SUPFAMiSSF53597. SSF53597. 1 hit.
PROSITEiPS00075. DHFR_1. 1 hit.
PS51330. DHFR_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDYR_SHV21
AccessioniPrimary (citable) accession number: P09503
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: October 5, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.