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Protein

Viral dihydrofolate reductase

Gene

DHFR

Organism
Saimiriine herpesvirus 2 (strain 11) (SaHV-2) (Herpesvirus saimiri)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis (By similarity).By similarity

Catalytic activityi

5,6,7,8-tetrahydrofolate + NADP+ = 7,8-dihydrofolate + NADPH.PROSITE-ProRule annotation

Pathwayi: tetrahydrofolate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate.
Proteins known to be involved in this subpathway in this organism are:
  1. Viral dihydrofolate reductase (DHFR)
This subpathway is part of the pathway tetrahydrofolate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5,6,7,8-tetrahydrofolate from 7,8-dihydrofolate, the pathway tetrahydrofolate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei10NADP; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei71SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi16 – 22NADPBy similarity7
Nucleotide bindingi55 – 57NADPBy similarity3
Nucleotide bindingi77 – 79NADPBy similarity3
Nucleotide bindingi117 – 124NADPBy similarity8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processOne-carbon metabolism
LigandNADP

Enzyme and pathway databases

UniPathwayiUPA00077; UER00158

Names & Taxonomyi

Protein namesi
Recommended name:
Viral dihydrofolate reductase (EC:1.5.1.3)
Short name:
vDHFR
Gene namesi
Name:DHFR
Synonyms:2
OrganismiSaimiriine herpesvirus 2 (strain 11) (SaHV-2) (Herpesvirus saimiri)
Taxonomic identifieri10383 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeGammaherpesvirinaeRhadinovirus
Virus hostiSaimiri sciureus (Common squirrel monkey) [TaxID: 9521]
Proteomesi
  • UP000000587 Componenti: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001863801 – 187Viral dihydrofolate reductaseAdd BLAST187

Proteomic databases

PRIDEiP09503

Structurei

3D structure databases

ProteinModelPortaliP09503
SMRiP09503
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini4 – 185DHFRPROSITE-ProRule annotationAdd BLAST182

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni31 – 36Substrate bindingBy similarity6

Sequence similaritiesi

Belongs to the dihydrofolate reductase family.Curated

Phylogenomic databases

OrthoDBiVOG090001MI

Family and domain databases

CDDicd00209 DHFR, 1 hit
Gene3Di3.40.430.101 hit
InterProiView protein in InterPro
IPR024072 DHFR-like_dom_sf
IPR017925 DHFR_CS
IPR001796 DHFR_dom
PfamiView protein in Pfam
PF00186 DHFR_1, 1 hit
SUPFAMiSSF53597 SSF53597, 1 hit
PROSITEiView protein in PROSITE
PS00075 DHFR_1, 1 hit
PS51330 DHFR_2, 1 hit

Sequencei

Sequence statusi: Complete.

P09503-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVQALNCIVA VAQNMGIGKQ GNLPWPRLMN DFKHFQRMTT TSSVPDKQNL
60 70 80 90 100
VIMGKKTWFS IPEKNRPLKG RINVVLSKEL KELPHRAHFL AKSLDDALKL
110 120 130 140 150
TEQPELANKV DMVWIIGGSS VYKEAMSYPC DLKLFVTRIM QDFECDTFFP
160 170 180
EFDLEKYKLL IEYPSVLSNV QEEKSIKYKF EVYEKNH
Length:187
Mass (Da):21,719
Last modified:July 1, 1989 - v1
Checksum:i46666EAA3BEA5140
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X64346 Genomic DNA Translation: CAA45624.1
M28071 Genomic DNA Translation: AAA58726.1
M19237 Genomic DNA Translation: AAA46154.1
RefSeqiNP_040203.1, NC_001350.1

Genome annotation databases

GeneIDi1488256
KEGGivg:1488256

Similar proteinsi

Entry informationi

Entry nameiDYR_SHV21
AccessioniPrimary (citable) accession number: P09503
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: March 28, 2018
This is version 93 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome