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P09497

- CLCB_HUMAN

UniProt

P09497 - CLCB_HUMAN

Protein

Clathrin light chain B

Gene

CLTB

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 132 (01 Oct 2014)
      Sequence version 1 (01 Jul 1989)
      Previous versions | rss
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    Functioni

    Clathrin is the major protein of the polyhedral coat of coated pits and vesicles.

    GO - Molecular functioni

    1. peptide binding Source: Ensembl
    2. protein binding Source: UniProtKB
    3. structural molecule activity Source: InterPro

    GO - Biological processi

    1. intracellular protein transport Source: InterPro
    2. vesicle-mediated transport Source: InterPro

    Keywords - Ligandi

    Calcium

    Enzyme and pathway databases

    ReactomeiREACT_11035. Gap junction degradation.
    REACT_11049. Formation of annular gap junctions.
    REACT_172599. WNT5A-dependent internalization of FZD4.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Clathrin light chain B
    Short name:
    Lcb
    Gene namesi
    Name:CLTB
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 5

    Organism-specific databases

    HGNCiHGNC:2091. CLTB.

    Subcellular locationi

    GO - Cellular componenti

    1. ciliary membrane Source: Ensembl
    2. clathrin coat Source: UniProtKB
    3. clathrin coat of coated pit Source: InterPro
    4. clathrin coat of trans-Golgi network vesicle Source: InterPro
    5. trans-Golgi network Source: Ensembl

    Keywords - Cellular componenti

    Coated pit, Cytoplasmic vesicle, Membrane

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA26617.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 229229Clathrin light chain BPRO_0000205771Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei11 – 111PhosphoserineBy similarity
    Modified residuei13 – 131PhosphoserineBy similarity
    Disulfide bondi199 ↔ 209By similarity
    Modified residuei204 – 2041N6-acetyllysineBy similarity

    Keywords - PTMi

    Acetylation, Disulfide bond, Phosphoprotein

    Proteomic databases

    MaxQBiP09497.
    PaxDbiP09497.
    PRIDEiP09497.

    2D gel databases

    OGPiP09497.

    PTM databases

    PhosphoSiteiP09497.

    Miscellaneous databases

    PMAP-CutDBP09497.

    Expressioni

    Gene expression databases

    ArrayExpressiP09497.
    BgeeiP09497.
    CleanExiHS_CLTB.
    GenevestigatoriP09497.

    Organism-specific databases

    HPAiCAB003692.

    Interactioni

    Subunit structurei

    Clathrin coats are formed from molecules containing 3 heavy chains and 3 light chains. Interacts (via N-terminus) with HIP1. Interacts with HIP1R.2 Publications

    Protein-protein interaction databases

    BioGridi107622. 5 interactions.
    IntActiP09497. 3 interactions.
    MINTiMINT-1390805.
    STRINGi9606.ENSP00000309415.

    Structurei

    3D structure databases

    ProteinModelPortaliP09497.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni93 – 15563Involved in binding clathrin heavy chainAdd
    BLAST

    Sequence similaritiesi

    Belongs to the clathrin light chain family.Curated

    Phylogenomic databases

    eggNOGiNOG312397.
    HOGENOMiHOG000008147.
    HOVERGENiHBG003386.
    InParanoidiP09497.
    KOiK04645.
    OMAiVMEQEWR.
    OrthoDBiEOG79KPH4.
    PhylomeDBiP09497.
    TreeFamiTF313162.

    Family and domain databases

    InterProiIPR000996. Clathrin_L-chain.
    [Graphical view]
    PANTHERiPTHR10639. PTHR10639. 1 hit.
    PfamiPF01086. Clathrin_lg_ch. 1 hit.
    [Graphical view]
    PROSITEiPS00224. CLATHRIN_LIGHT_CHN_1. 1 hit.
    PS00581. CLATHRIN_LIGHT_CHN_2. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform Brain (identifier: P09497-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MADDFGFFSS SESGAPEAAE EDPAAAFLAQ QESEIAGIEN DEGFGAPAGS    50
    HAAPAQPGPT SGAGSEDMGT TVNGDVFQEA NGPADGYAAI AQADRLTQEP 100
    ESIRKWREEQ RKRLQELDAA SKVTEQEWRE KAKKDLEEWN QRQSEQVEKN 150
    KINNRIADKA FYQQPDADII GYVASEEAFV KESKEETPGT EWEKVAQLCD 200
    FNPKSSKQCK DVSRLRSVLM SLKQTPLSR 229
    Length:229
    Mass (Da):25,190
    Last modified:July 1, 1989 - v1
    Checksum:i07DBA19D44648F6F
    GO
    Isoform Non-brain (identifier: P09497-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         156-173: Missing.

    Show »
    Length:211
    Mass (Da):23,181
    Checksum:i5B40F043E1809238
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti5 – 51F → I in CAG38814. 1 PublicationCurated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei156 – 17318Missing in isoform Non-brain. 3 PublicationsVSP_001098Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M20469 mRNA. Translation: AAA51818.1.
    M20470 mRNA. Translation: AAA59506.1.
    BT007028 mRNA. Translation: AAP35675.1.
    CR536577 mRNA. Translation: CAG38814.1.
    CH471195 Genomic DNA. Translation: EAW85079.1.
    BC006332 mRNA. Translation: AAH06332.1.
    BC006457 mRNA. Translation: AAH06457.1.
    CCDSiCCDS4402.1. [P09497-2]
    CCDS4403.1. [P09497-1]
    PIRiB31775.
    RefSeqiNP_001825.1. NM_001834.3. [P09497-2]
    NP_009028.1. NM_007097.3. [P09497-1]
    UniGeneiHs.484241.

    Genome annotation databases

    EnsembliENST00000310418; ENSP00000309415; ENSG00000175416. [P09497-1]
    ENST00000345807; ENSP00000310812; ENSG00000175416. [P09497-2]
    GeneIDi1212.
    KEGGihsa:1212.
    UCSCiuc003meh.4. human. [P09497-1]
    uc003mei.4. human. [P09497-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M20469 mRNA. Translation: AAA51818.1 .
    M20470 mRNA. Translation: AAA59506.1 .
    BT007028 mRNA. Translation: AAP35675.1 .
    CR536577 mRNA. Translation: CAG38814.1 .
    CH471195 Genomic DNA. Translation: EAW85079.1 .
    BC006332 mRNA. Translation: AAH06332.1 .
    BC006457 mRNA. Translation: AAH06457.1 .
    CCDSi CCDS4402.1. [P09497-2 ]
    CCDS4403.1. [P09497-1 ]
    PIRi B31775.
    RefSeqi NP_001825.1. NM_001834.3. [P09497-2 ]
    NP_009028.1. NM_007097.3. [P09497-1 ]
    UniGenei Hs.484241.

    3D structure databases

    ProteinModelPortali P09497.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 107622. 5 interactions.
    IntActi P09497. 3 interactions.
    MINTi MINT-1390805.
    STRINGi 9606.ENSP00000309415.

    PTM databases

    PhosphoSitei P09497.

    2D gel databases

    OGPi P09497.

    Proteomic databases

    MaxQBi P09497.
    PaxDbi P09497.
    PRIDEi P09497.

    Protocols and materials databases

    DNASUi 1212.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000310418 ; ENSP00000309415 ; ENSG00000175416 . [P09497-1 ]
    ENST00000345807 ; ENSP00000310812 ; ENSG00000175416 . [P09497-2 ]
    GeneIDi 1212.
    KEGGi hsa:1212.
    UCSCi uc003meh.4. human. [P09497-1 ]
    uc003mei.4. human. [P09497-2 ]

    Organism-specific databases

    CTDi 1212.
    GeneCardsi GC05M175819.
    HGNCi HGNC:2091. CLTB.
    HPAi CAB003692.
    MIMi 118970. gene.
    neXtProti NX_P09497.
    PharmGKBi PA26617.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi NOG312397.
    HOGENOMi HOG000008147.
    HOVERGENi HBG003386.
    InParanoidi P09497.
    KOi K04645.
    OMAi VMEQEWR.
    OrthoDBi EOG79KPH4.
    PhylomeDBi P09497.
    TreeFami TF313162.

    Enzyme and pathway databases

    Reactomei REACT_11035. Gap junction degradation.
    REACT_11049. Formation of annular gap junctions.
    REACT_172599. WNT5A-dependent internalization of FZD4.

    Miscellaneous databases

    ChiTaRSi CLTB. human.
    GenomeRNAii 1212.
    NextBioi 4993.
    PMAP-CutDB P09497.
    PROi P09497.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P09497.
    Bgeei P09497.
    CleanExi HS_CLTB.
    Genevestigatori P09497.

    Family and domain databases

    InterProi IPR000996. Clathrin_L-chain.
    [Graphical view ]
    PANTHERi PTHR10639. PTHR10639. 1 hit.
    Pfami PF01086. Clathrin_lg_ch. 1 hit.
    [Graphical view ]
    PROSITEi PS00224. CLATHRIN_LIGHT_CHN_1. 1 hit.
    PS00581. CLATHRIN_LIGHT_CHN_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure of human clathrin light chains. Conservation of light chain polymorphism in three mammalian species."
      Jackson A.P., Parham P.
      J. Biol. Chem. 263:16688-16695(1988) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
    2. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
      Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
      Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM NON-BRAIN).
    3. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
      Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J.
      Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM NON-BRAIN).
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM NON-BRAIN).
      Tissue: Lung and Retinal pigment epithelium.
    6. "HIP1 and HIP12 display differential binding to F-actin, AP2, and clathrin. Identification of a novel interaction with clathrin light chain."
      Legendre-Guillemin V., Metzler M., Charbonneau M., Gan L., Chopra V., Philie J., Hayden M.R., McPherson P.S.
      J. Biol. Chem. 277:19897-19904(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HIP1 AND HIP1R.
    7. "Huntingtin-interacting protein 1 (Hip1) and Hip1-related protein (Hip1R) bind the conserved sequence of clathrin light chains and thereby influence clathrin assembly in vitro and actin distribution in vivo."
      Chen C.-Y., Brodsky F.M.
      J. Biol. Chem. 280:6109-6117(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH HIP1R.
    8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiCLCB_HUMAN
    AccessioniPrimary (citable) accession number: P09497
    Secondary accession number(s): Q53Y37, Q6FHW1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: July 1, 1989
    Last modified: October 1, 2014
    This is version 132 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Human chromosome 5
      Human chromosome 5: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3