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Protein

Clathrin light chain B

Gene

CLTB

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles.

GO - Molecular functioni

  1. peptide binding Source: Ensembl
  2. structural molecule activity Source: InterPro

GO - Biological processi

  1. intracellular protein transport Source: InterPro
  2. vesicle-mediated transport Source: InterPro
Complete GO annotation...

Keywords - Ligandi

Calcium

Enzyme and pathway databases

ReactomeiREACT_11035. Gap junction degradation.
REACT_11049. Formation of annular gap junctions.
REACT_172599. WNT5A-dependent internalization of FZD4.
REACT_228189. EPH-ephrin mediated repulsion of cells.

Names & Taxonomyi

Protein namesi
Recommended name:
Clathrin light chain B
Short name:
Lcb
Gene namesi
Name:CLTB
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 5

Organism-specific databases

HGNCiHGNC:2091. CLTB.

Subcellular locationi

GO - Cellular componenti

  1. ciliary membrane Source: Ensembl
  2. clathrin coat Source: UniProtKB
  3. clathrin coat of coated pit Source: InterPro
  4. clathrin coat of trans-Golgi network vesicle Source: InterPro
  5. cytoplasm Source: HPA
  6. intracellular membrane-bounded organelle Source: HPA
  7. plasma membrane Source: HPA
  8. trans-Golgi network Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Coated pit, Cytoplasmic vesicle, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA26617.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 229229Clathrin light chain BPRO_0000205771Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei11 – 111PhosphoserineBy similarity
Modified residuei13 – 131PhosphoserineBy similarity
Disulfide bondi199 ↔ 209By similarity
Modified residuei204 – 2041N6-acetyllysineBy similarity

Keywords - PTMi

Acetylation, Disulfide bond, Phosphoprotein

Proteomic databases

MaxQBiP09497.
PaxDbiP09497.
PRIDEiP09497.

2D gel databases

OGPiP09497.

PTM databases

PhosphoSiteiP09497.

Miscellaneous databases

PMAP-CutDBP09497.

Expressioni

Gene expression databases

BgeeiP09497.
CleanExiHS_CLTB.
ExpressionAtlasiP09497. baseline and differential.
GenevestigatoriP09497.

Organism-specific databases

HPAiCAB003692.

Interactioni

Subunit structurei

Clathrin coats are formed from molecules containing 3 heavy chains and 3 light chains. Interacts (via N-terminus) with HIP1. Interacts with HIP1R.2 Publications

Protein-protein interaction databases

BioGridi107622. 6 interactions.
IntActiP09497. 3 interactions.
MINTiMINT-1390805.
STRINGi9606.ENSP00000309415.

Structurei

3D structure databases

ProteinModelPortaliP09497.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni93 – 15563Involved in binding clathrin heavy chainAdd
BLAST

Sequence similaritiesi

Belongs to the clathrin light chain family.Curated

Phylogenomic databases

eggNOGiNOG312397.
GeneTreeiENSGT00390000010441.
HOGENOMiHOG000008147.
HOVERGENiHBG003386.
InParanoidiP09497.
KOiK04645.
OMAiVMEQEWR.
OrthoDBiEOG79KPH4.
PhylomeDBiP09497.
TreeFamiTF313162.

Family and domain databases

InterProiIPR000996. Clathrin_L-chain.
[Graphical view]
PANTHERiPTHR10639. PTHR10639. 1 hit.
PfamiPF01086. Clathrin_lg_ch. 1 hit.
[Graphical view]
PROSITEiPS00224. CLATHRIN_LIGHT_CHN_1. 1 hit.
PS00581. CLATHRIN_LIGHT_CHN_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform Brain (identifier: P09497-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MADDFGFFSS SESGAPEAAE EDPAAAFLAQ QESEIAGIEN DEGFGAPAGS
60 70 80 90 100
HAAPAQPGPT SGAGSEDMGT TVNGDVFQEA NGPADGYAAI AQADRLTQEP
110 120 130 140 150
ESIRKWREEQ RKRLQELDAA SKVTEQEWRE KAKKDLEEWN QRQSEQVEKN
160 170 180 190 200
KINNRIADKA FYQQPDADII GYVASEEAFV KESKEETPGT EWEKVAQLCD
210 220
FNPKSSKQCK DVSRLRSVLM SLKQTPLSR
Length:229
Mass (Da):25,190
Last modified:July 1, 1989 - v1
Checksum:i07DBA19D44648F6F
GO
Isoform Non-brain (identifier: P09497-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     156-173: Missing.

Show »
Length:211
Mass (Da):23,181
Checksum:i5B40F043E1809238
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti5 – 51F → I in CAG38814. 1 PublicationCurated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei156 – 17318Missing in isoform Non-brain. 3 PublicationsVSP_001098Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20469 mRNA. Translation: AAA51818.1.
M20470 mRNA. Translation: AAA59506.1.
BT007028 mRNA. Translation: AAP35675.1.
CR536577 mRNA. Translation: CAG38814.1.
CH471195 Genomic DNA. Translation: EAW85079.1.
BC006332 mRNA. Translation: AAH06332.1.
BC006457 mRNA. Translation: AAH06457.1.
CCDSiCCDS4402.1. [P09497-2]
CCDS4403.1. [P09497-1]
PIRiB31775.
RefSeqiNP_001825.1. NM_001834.3. [P09497-2]
NP_009028.1. NM_007097.3. [P09497-1]
UniGeneiHs.484241.

Genome annotation databases

EnsembliENST00000310418; ENSP00000309415; ENSG00000175416. [P09497-1]
ENST00000345807; ENSP00000310812; ENSG00000175416. [P09497-2]
GeneIDi1212.
KEGGihsa:1212.
UCSCiuc003meh.4. human. [P09497-1]
uc003mei.4. human. [P09497-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M20469 mRNA. Translation: AAA51818.1.
M20470 mRNA. Translation: AAA59506.1.
BT007028 mRNA. Translation: AAP35675.1.
CR536577 mRNA. Translation: CAG38814.1.
CH471195 Genomic DNA. Translation: EAW85079.1.
BC006332 mRNA. Translation: AAH06332.1.
BC006457 mRNA. Translation: AAH06457.1.
CCDSiCCDS4402.1. [P09497-2]
CCDS4403.1. [P09497-1]
PIRiB31775.
RefSeqiNP_001825.1. NM_001834.3. [P09497-2]
NP_009028.1. NM_007097.3. [P09497-1]
UniGeneiHs.484241.

3D structure databases

ProteinModelPortaliP09497.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi107622. 6 interactions.
IntActiP09497. 3 interactions.
MINTiMINT-1390805.
STRINGi9606.ENSP00000309415.

PTM databases

PhosphoSiteiP09497.

2D gel databases

OGPiP09497.

Proteomic databases

MaxQBiP09497.
PaxDbiP09497.
PRIDEiP09497.

Protocols and materials databases

DNASUi1212.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000310418; ENSP00000309415; ENSG00000175416. [P09497-1]
ENST00000345807; ENSP00000310812; ENSG00000175416. [P09497-2]
GeneIDi1212.
KEGGihsa:1212.
UCSCiuc003meh.4. human. [P09497-1]
uc003mei.4. human. [P09497-2]

Organism-specific databases

CTDi1212.
GeneCardsiGC05M175819.
HGNCiHGNC:2091. CLTB.
HPAiCAB003692.
MIMi118970. gene.
neXtProtiNX_P09497.
PharmGKBiPA26617.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiNOG312397.
GeneTreeiENSGT00390000010441.
HOGENOMiHOG000008147.
HOVERGENiHBG003386.
InParanoidiP09497.
KOiK04645.
OMAiVMEQEWR.
OrthoDBiEOG79KPH4.
PhylomeDBiP09497.
TreeFamiTF313162.

Enzyme and pathway databases

ReactomeiREACT_11035. Gap junction degradation.
REACT_11049. Formation of annular gap junctions.
REACT_172599. WNT5A-dependent internalization of FZD4.
REACT_228189. EPH-ephrin mediated repulsion of cells.

Miscellaneous databases

ChiTaRSiCLTB. human.
GenomeRNAii1212.
NextBioi4993.
PMAP-CutDBP09497.
PROiP09497.
SOURCEiSearch...

Gene expression databases

BgeeiP09497.
CleanExiHS_CLTB.
ExpressionAtlasiP09497. baseline and differential.
GenevestigatoriP09497.

Family and domain databases

InterProiIPR000996. Clathrin_L-chain.
[Graphical view]
PANTHERiPTHR10639. PTHR10639. 1 hit.
PfamiPF01086. Clathrin_lg_ch. 1 hit.
[Graphical view]
PROSITEiPS00224. CLATHRIN_LIGHT_CHN_1. 1 hit.
PS00581. CLATHRIN_LIGHT_CHN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure of human clathrin light chains. Conservation of light chain polymorphism in three mammalian species."
    Jackson A.P., Parham P.
    J. Biol. Chem. 263:16688-16695(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
  2. "Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
    Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
    Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM NON-BRAIN).
  3. "Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
    Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J.
    Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM NON-BRAIN).
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM NON-BRAIN).
    Tissue: Lung and Retinal pigment epithelium.
  6. "HIP1 and HIP12 display differential binding to F-actin, AP2, and clathrin. Identification of a novel interaction with clathrin light chain."
    Legendre-Guillemin V., Metzler M., Charbonneau M., Gan L., Chopra V., Philie J., Hayden M.R., McPherson P.S.
    J. Biol. Chem. 277:19897-19904(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HIP1 AND HIP1R.
  7. "Huntingtin-interacting protein 1 (Hip1) and Hip1-related protein (Hip1R) bind the conserved sequence of clathrin light chains and thereby influence clathrin assembly in vitro and actin distribution in vivo."
    Chen C.-Y., Brodsky F.M.
    J. Biol. Chem. 280:6109-6117(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH HIP1R.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiCLCB_HUMAN
AccessioniPrimary (citable) accession number: P09497
Secondary accession number(s): Q53Y37, Q6FHW1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: February 4, 2015
This is version 136 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 5
    Human chromosome 5: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.