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P09497 (CLCB_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified March 19, 2014. Version 126. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Clathrin light chain B

Short name=Lcb
Gene names
Name:CLTB
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length229 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles.

Subunit structure

Clathrin coats are formed from molecules containing 3 heavy chains and 3 light chains. Interacts (via N-terminus) with HIP1. Interacts with HIP1R. Ref.6 Ref.7

Subcellular location

Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Membranecoated pit; Peripheral membrane protein; Cytoplasmic side. Note: Cytoplasmic face of coated pits and vesicles.

Sequence similarities

Belongs to the clathrin light chain family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Brain (identifier: P09497-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Non-brain (identifier: P09497-2)

The sequence of this isoform differs from the canonical sequence as follows:
     156-173: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 229229Clathrin light chain B
PRO_0000205771

Regions

Region93 – 15563Involved in binding clathrin heavy chain

Amino acid modifications

Modified residue111Phosphoserine By similarity
Modified residue131Phosphoserine By similarity
Modified residue2041N6-acetyllysine By similarity
Disulfide bond199 ↔ 209 By similarity

Natural variations

Alternative sequence156 – 17318Missing in isoform Non-brain.
VSP_001098

Experimental info

Sequence conflict51F → I in CAG38814. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform Brain [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 07DBA19D44648F6F

FASTA22925,190
        10         20         30         40         50         60 
MADDFGFFSS SESGAPEAAE EDPAAAFLAQ QESEIAGIEN DEGFGAPAGS HAAPAQPGPT 

        70         80         90        100        110        120 
SGAGSEDMGT TVNGDVFQEA NGPADGYAAI AQADRLTQEP ESIRKWREEQ RKRLQELDAA 

       130        140        150        160        170        180 
SKVTEQEWRE KAKKDLEEWN QRQSEQVEKN KINNRIADKA FYQQPDADII GYVASEEAFV 

       190        200        210        220 
KESKEETPGT EWEKVAQLCD FNPKSSKQCK DVSRLRSVLM SLKQTPLSR 

« Hide

Isoform Non-brain [UniParc].

Checksum: 5B40F043E1809238
Show »

FASTA21123,181

References

« Hide 'large scale' references
[1]"Structure of human clathrin light chains. Conservation of light chain polymorphism in three mammalian species."
Jackson A.P., Parham P.
J. Biol. Chem. 263:16688-16695(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
[2]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM NON-BRAIN).
[3]"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)."
Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J.
Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM NON-BRAIN).
[4]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM NON-BRAIN).
Tissue: Lung and Retinal pigment epithelium.
[6]"HIP1 and HIP12 display differential binding to F-actin, AP2, and clathrin. Identification of a novel interaction with clathrin light chain."
Legendre-Guillemin V., Metzler M., Charbonneau M., Gan L., Chopra V., Philie J., Hayden M.R., McPherson P.S.
J. Biol. Chem. 277:19897-19904(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH HIP1 AND HIP1R.
[7]"Huntingtin-interacting protein 1 (Hip1) and Hip1-related protein (Hip1R) bind the conserved sequence of clathrin light chains and thereby influence clathrin assembly in vitro and actin distribution in vivo."
Chen C.-Y., Brodsky F.M.
J. Biol. Chem. 280:6109-6117(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH HIP1R.
[8]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M20469 mRNA. Translation: AAA51818.1.
M20470 mRNA. Translation: AAA59506.1.
BT007028 mRNA. Translation: AAP35675.1.
CR536577 mRNA. Translation: CAG38814.1.
CH471195 Genomic DNA. Translation: EAW85079.1.
BC006332 mRNA. Translation: AAH06332.1.
BC006457 mRNA. Translation: AAH06457.1.
PIRB31775.
RefSeqNP_001825.1. NM_001834.3.
NP_009028.1. NM_007097.3.
UniGeneHs.484241.

3D structure databases

ProteinModelPortalP09497.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid107622. 4 interactions.
IntActP09497. 3 interactions.
MINTMINT-1390805.
STRING9606.ENSP00000309415.

PTM databases

PhosphoSiteP09497.

Polymorphism databases

DMDM116505.

2D gel databases

OGPP09497.

Proteomic databases

PaxDbP09497.
PRIDEP09497.

Protocols and materials databases

DNASU1212.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000310418; ENSP00000309415; ENSG00000175416. [P09497-1]
ENST00000345807; ENSP00000310812; ENSG00000175416. [P09497-2]
GeneID1212.
KEGGhsa:1212.
UCSCuc003meh.4. human. [P09497-1]
uc003mei.4. human. [P09497-2]

Organism-specific databases

CTD1212.
GeneCardsGC05M175819.
HGNCHGNC:2091. CLTB.
HPACAB003692.
MIM118970. gene.
neXtProtNX_P09497.
PharmGKBPA26617.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG312397.
HOGENOMHOG000008147.
HOVERGENHBG003386.
InParanoidP09497.
KOK04645.
OMAVMEQEWR.
OrthoDBEOG79KPH4.
PhylomeDBP09497.
TreeFamTF313162.

Enzyme and pathway databases

ReactomeREACT_111102. Signal Transduction.
REACT_11123. Membrane Trafficking.

Gene expression databases

ArrayExpressP09497.
BgeeP09497.
CleanExHS_CLTB.
GenevestigatorP09497.

Family and domain databases

InterProIPR000996. Clathrin_L-chain.
[Graphical view]
PANTHERPTHR10639. PTHR10639. 1 hit.
PfamPF01086. Clathrin_lg_ch. 1 hit.
[Graphical view]
PROSITEPS00224. CLATHRIN_LIGHT_CHN_1. 1 hit.
PS00581. CLATHRIN_LIGHT_CHN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSCLTB. human.
GenomeRNAi1212.
NextBio4993.
PMAP-CutDBP09497.
PROP09497.
SOURCESearch...

Entry information

Entry nameCLCB_HUMAN
AccessionPrimary (citable) accession number: P09497
Secondary accession number(s): Q53Y37, Q6FHW1
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: March 19, 2014
This is version 126 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 5

Human chromosome 5: entries, gene names and cross-references to MIM