Reviewed, UniProtKB/Swiss-Prot P09497 (CLCB_HUMAN)

Names and origin

Protein names

Recommended name:
    Clathrin light chain B
      Short name=Lcb

Gene names

Name:

CLTB

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	229 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Clathrin is the major protein of the polyhedral coat of coated pits and vesicles.
Subunit structure	Clathrin coats are formed from molecules containing 3 heavy chains and 3 light chains. Interacts (via N-terminus) with HIP1. Interacts with HIP1R. Ref.6 Ref.7
Subcellular location	Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Membrane › coated pit; Peripheral membrane protein; Cytoplasmic side. Note: Cytoplasmic face of coated pits and vesicles.

Ontologies

Keywords
Cellular component	Coated pit Cytoplasmic vesicle Membrane
Coding sequence diversity	Alternative splicing
Ligand	Calcium
PTM	Disulfide bond Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	intracellular protein transport Non-traceable author statement. Source: UniProtKB vesicle-mediated transport Inferred from electronic annotation. Source: InterPro
Cellular component	clathrin coat of coated pit Inferred from electronic annotation. Source: InterPro clathrin coat of trans-Golgi network vesicle Inferred from electronic annotation. Source: InterPro
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Ref.6 Ref.7 Inferred from physical interaction. Source: UniProtKB structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Brain (identifier: P09497-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Non-brain (identifier: P09497-2)

The sequence of this isoform differs from the canonical sequence as follows:
     156-173: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 229	229	Clathrin light chain B		PRO_0000205771
Regions
	Region	93 – 155	63	Involved in binding clathrin heavy chain
Amino acid modifications
	Modified residue	11	1	Phosphoserine By similarity
	Modified residue	13	1	Phosphoserine By similarity
	Disulfide bond	199 ↔ 209		By similarity
Natural variations
	Alternative sequence	156 – 173	18	Missing in isoform Non-brain.		VSP_001098
Experimental info
	Sequence conflict	5	1	F → I in CAG38814. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	Isoform Brain [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 07DBA19D44648F6F	FASTA	229	25,190
	10 20 30 40 50 60 MADDFGFFSS SESGAPEAAE EDPAAAFLAQ QESEIAGIEN DEGFGAPAGS HAAPAQPGPT 70 80 90 100 110 120 SGAGSEDMGT TVNGDVFQEA NGPADGYAAI AQADRLTQEP ESIRKWREEQ RKRLQELDAA 130 140 150 160 170 180 SKVTEQEWRE KAKKDLEEWN QRQSEQVEKN KINNRIADKA FYQQPDADII GYVASEEAFV 190 200 210 220 KESKEETPGT EWEKVAQLCD FNPKSSKQCK DVSRLRSVLM SLKQTPLSR
	Isoform Non-brain. Checksum: 5B40F043E1809238	FASTA	211	23,181
	10 20 30 40 50 60 MADDFGFFSS SESGAPEAAE EDPAAAFLAQ QESEIAGIEN DEGFGAPAGS HAAPAQPGPT 70 80 90 100 110 120 SGAGSEDMGT TVNGDVFQEA NGPADGYAAI AQADRLTQEP ESIRKWREEQ RKRLQELDAA 130 140 150 160 170 180 SKVTEQEWRE KAKKDLEEWN QRQSEQVEKN KINNRASEEA FVKESKEETP GTEWEKVAQL 190 200 210 CDFNPKSSKQ CKDVSRLRSV LMSLKQTPLS R

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure of human clathrin light chains. Conservation of light chain polymorphism in three mammalian species." Jackson A.P., Parham P. J. Biol. Chem. 263:16688-16695(1988) [PubMed: 3267234] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
[2]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM NON-BRAIN).
[3]	"Cloning of human full open reading frames in Gateway(TM) system entry vector (pDONR201)." Halleck A., Ebert L., Mkoundinya M., Schick M., Eisenstein S., Neubert P., Kstrang K., Schatten R., Shen B., Henze S., Mar W., Korn B., Zuo D., Hu Y., LaBaer J. Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM NON-BRAIN).
[4]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM NON-BRAIN). Tissue: Lung and Retinal pigment epithelium.
[6]	"HIP1 and HIP12 display differential binding to F-actin, AP2, and clathrin. Identification of a novel interaction with clathrin light chain." Legendre-Guillemin V., Metzler M., Charbonneau M., Gan L., Chopra V., Philie J., Hayden M.R., McPherson P.S. J. Biol. Chem. 277:19897-19904(2002) [PubMed: 11889126] [Abstract] Cited for: INTERACTION WITH HIP1 AND HIP1R.
[7]	"Huntingtin-interacting protein 1 (Hip1) and Hip1-related protein (Hip1R) bind the conserved sequence of clathrin light chains and thereby influence clathrin assembly in vitro and actin distribution in vivo." Chen C.-Y., Brodsky F.M. J. Biol. Chem. 280:6109-6117(2005) [PubMed: 15533940] [Abstract] Cited for: INTERACTION WITH HIP1R.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M20469 mRNA. Translation: AAA51818.1. M20470 mRNA. Translation: AAA59506.1. BT007028 mRNA. Translation: AAP35675.1. CR536577 mRNA. Translation: CAG38814.1. CH471195 Genomic DNA. Translation: EAW85079.1. BC006332 mRNA. Translation: AAH06332.1. BC006457 mRNA. Translation: AAH06457.1.
IPI	IPI00014589. IPI00216472.
PIR	B31775.
RefSeq	NP_001825.1. NP_009028.1.
UniGene	Hs.484241
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P09497. 2 interactions.
STRING	P09497.
PTM databases
PhosphoSite	P09497.
2-D gel databases
OGP	P09497.
Proteomic databases
PRIDE	P09497.
Genome annotation databases
Ensembl	ENST00000310418; ENSP00000309415; ENSG00000175416; Homo sapiens. [Genome view]
GeneID	1212.
KEGG	hsa:1212.
UCSC	uc003meh.1. human.
Organism-specific databases
CTD	1212.
GeneCards	GC05M175752.
H-InvDB	HIX0005441.
HGNC	HGNC:2091. CLTB.
HPA	CAB003692.
MIM	118970. gene.
PharmGKB	PA26617.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	P09497.
HOVERGEN	P09497.
OMA	INNRIAD
OrthoDB	EOG9H74X9
Enzyme and pathway databases
Pathway_Interaction_DB	arf_3pathway. Arf1 pathway.
Reactome	REACT_9480. Gap junction trafficking and regulation.
Gene expression databases
ArrayExpress	P09497.
Bgee	P09497.
CleanEx	HS_CLTB.
Genevestigator	P09497.
GermOnline	ENSG00000175416. Homo sapiens.
Family and domain databases
InterPro	IPR000996. Clathrin_L-chain. [Graphical view]
PANTHER	PTHR10639. Clathrin_lg_ch. 1 hit.
Pfam	PF01086. Clathrin_lg_ch. 1 hit. [Graphical view]
PROSITE	PS00224. CLATHRIN_LIGHT_CHN_1. 1 hit. PS00581. CLATHRIN_LIGHT_CHN_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	4993.
PMAP-CutDB	P09497.
SOURCE	Search...

Entry information

Entry name

CLCB_HUMAN

Accession

Primary (citable) accession number: P09497
Secondary accession number(s): Q53Y37, Q6FHW1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	November 24, 2009
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.