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P09496 (CLCA_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 140. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Interactions·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Clathrin light chain A

Short name=Lca
Gene names
Name:CLTA
OrganismHomo sapiens (Human) [Reference proteome]
Taxonomic identifier9606 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo

Protein attributes

Sequence length248 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Clathrin is the major protein of the polyhedral coat of coated pits and vesicles.

Subunit structure

Clathrin coats are formed from molecules containing 3 heavy chains and 3 light chains. Interacts with CALY; the interaction stimulates clathrin self-assembly and clathrin-mediated endocytosis. Ref.8

Subcellular location

Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Membranecoated pit; Peripheral membrane protein; Cytoplasmic side. Note: Cytoplasmic face of coated pits and vesicles.

Sequence similarities

Belongs to the clathrin light chain family.

Ontologies

Keywords
   Cellular componentCoated pit
Cytoplasmic vesicle
Membrane
   Coding sequence diversityAlternative splicing
   LigandCalcium
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processantigen processing and presentation of exogenous peptide antigen via MHC class II

Traceable author statement. Source: Reactome

axon guidance

Traceable author statement. Source: Reactome

endocytosis

Inferred from electronic annotation. Source: Ensembl

epidermal growth factor receptor signaling pathway

Traceable author statement. Source: Reactome

intracellular protein transport

Inferred from electronic annotation. Source: InterPro

membrane organization

Traceable author statement. Source: Reactome

negative regulation of epidermal growth factor receptor signaling pathway

Traceable author statement. Source: Reactome

neurotrophin TRK receptor signaling pathway

Traceable author statement. Source: Reactome

post-Golgi vesicle-mediated transport

Traceable author statement. Source: Reactome

   Cellular_componentclathrin coat

Non-traceable author statement Ref.1. Source: UniProtKB

clathrin coat of coated pit

Inferred from electronic annotation. Source: InterPro

clathrin coat of trans-Golgi network vesicle

Inferred from electronic annotation. Source: InterPro

clathrin complex

Inferred from direct assay PubMed 4066749. Source: FlyBase

clathrin-coated endocytic vesicle membrane

Traceable author statement. Source: Reactome

cytoplasmic membrane-bounded vesicle

Inferred from sequence or structural similarity. Source: BHF-UCL

cytosol

Traceable author statement. Source: Reactome

intracellular membrane-bounded organelle

Inferred from direct assay. Source: HPA

plasma membrane

Traceable author statement. Source: Reactome

trans-Golgi network membrane

Traceable author statement. Source: Reactome

   Molecular_functionclathrin heavy chain binding

Inferred from physical interaction PubMed 4066749. Source: FlyBase

peptide binding

Inferred from electronic annotation. Source: Ensembl

protein binding

Inferred from physical interaction PubMed 21297582. Source: IntAct

structural molecule activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Binary interactions

With

Entry

#Exp.

IntAct

Notes

TACC3Q9Y6A53EBI-1171169,EBI-2554984

Alternative products

This entry describes 5 isoforms produced by alternative splicing. [Align] [Select]
Isoform Brain (identifier: P09496-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Non-brain (identifier: P09496-2)

The sequence of this isoform differs from the canonical sequence as follows:
     163-192: Missing.
Isoform 3 (identifier: P09496-3)

The sequence of this isoform differs from the canonical sequence as follows:
     181-192: Missing.
Isoform 4 (identifier: P09496-4)

The sequence of this isoform differs from the canonical sequence as follows:
     162-180: RVADEAFYKQPFADVIGYV → S
Note: No experimental confirmation available.
Isoform 5 (identifier: P09496-5)

The sequence of this isoform differs from the canonical sequence as follows:
     73-124: Missing.
     163-192: Missing.
Note: No experimental confirmation available. Gene prediction based on EST data.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 248248Clathrin light chain A
PRO_0000205767

Regions

Region100 – 16263Involved in binding clathrin heavy chain

Amino acid modifications

Modified residue1051Phosphoserine Ref.12
Modified residue2061Phosphoserine Ref.10 Ref.12
Modified residue2361Phosphoserine Ref.10
Modified residue2421N6-acetyllysine By similarity

Natural variations

Alternative sequence73 – 12452Missing in isoform 5.
VSP_047168
Alternative sequence162 – 18019RVADE…VIGYV → S in isoform 4.
VSP_043239
Alternative sequence163 – 19230Missing in isoform Non-brain and isoform 5.
VSP_001095
Alternative sequence181 – 19212Missing in isoform 3.
VSP_024238

Experimental info

Sequence conflict2071P → Q in ABB76683. Ref.4

Sequences

Sequence LengthMass (Da)Tools
Isoform Brain [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 8D8A3B49E6353D93

FASTA24827,077
        10         20         30         40         50         60 
MAELDPFGAP AGAPGGPALG NGVAGAGEED PAAAFLAQQE SEIAGIENDE AFAILDGGAP 

        70         80         90        100        110        120 
GPQPHGEPPG GPDAVDGVMN GEYYQESNGP TDSYAAISQV DRLQSEPESI RKWREEQMER 

       130        140        150        160        170        180 
LEALDANSRK QEAEWKEKAI KELEEWYARQ DEQLQKTKAN NRVADEAFYK QPFADVIGYV 

       190        200        210        220        230        240 
TNINHPCYSL EQAAEEAFVN DIDESSPGTE WERVARLCDF NPKSSKQAKD VSRMRSVLIS 


LKQAPLVH 

« Hide

Isoform Non-brain [UniParc].

Checksum: 05C5037569FB6E8D
Show »

FASTA21823,662
Isoform 3 [UniParc].

Checksum: DAD1A01F3FD51284
Show »

FASTA23625,676
Isoform 4 [UniParc].

Checksum: B9644FCE2C09A116
Show »

FASTA23024,993
Isoform 5 [UniParc].

Checksum: 1F8381B9C1F55136
Show »

FASTA16617,687

References

« Hide 'large scale' references
[1]"Structure of human clathrin light chains. Conservation of light chain polymorphism in three mammalian species."
Jackson A.P., Parham P.
J. Biol. Chem. 263:16688-16695(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS BRAIN AND NON-BRAIN).
[2]"Cloning of human full-length CDSs in BD Creator(TM) system donor vector."
Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A.
Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM NON-BRAIN).
[3]"Complete sequencing and characterization of 21,243 full-length human cDNAs."
Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. expand/collapse author list , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS NON-BRAIN AND 4).
Tissue: Hippocampus.
[4]Lin L., Nong W., Zhou G., Ke R., Shen C., Zhong G., Zheng Z., Liang M., Tang Z., Huang B., Li H., Yang S.
Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
[5]"DNA sequence and analysis of human chromosome 9."
Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. expand/collapse author list , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I.
Nature 429:369-374(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. expand/collapse author list , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C.
Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM NON-BRAIN).
Tissue: Lung.
[8]"Calcyon, a novel partner of clathrin light chain, stimulates clathrin-mediated endocytosis."
Xiao J., Dai R., Negyessy L., Bergson C.
J. Biol. Chem. 281:15182-15193(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH CALY.
[9]"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle."
Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M.
Mol. Cell 31:438-448(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[10]"A quantitative atlas of mitotic phosphorylation."
Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P.
Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-206 AND SER-236, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[11]"Large-scale proteomics analysis of the human kinome."
Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H.
Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[12]"Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-105 AND SER-206, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Tissue: Cervix carcinoma.
[13]"Initial characterization of the human central proteome."
Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J.
BMC Syst. Biol. 5:17-17(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M20471 mRNA. Translation: AAA51817.1.
M20472 mRNA. Translation: AAA59505.1.
DQ270158 mRNA. Translation: ABB76683.1.
BT007170 mRNA. Translation: AAP35834.1.
AK291078 mRNA. Translation: BAF83767.1.
AK295692 mRNA. Translation: BAG58543.1.
AL158830 Genomic DNA. Translation: CAD13387.1.
AL158830 Genomic DNA. Translation: CAD13388.1.
AL161792 Genomic DNA. No translation available.
CH471071 Genomic DNA. Translation: EAW58312.1.
BC009201 mRNA. Translation: AAH09201.1.
BC019287 mRNA. Translation: AAH19287.1.
CCDSCCDS43802.1. [P09496-3]
CCDS55306.1. [P09496-4]
CCDS55307.1. [P09496-5]
CCDS6600.1. [P09496-2]
CCDS6601.1. [P09496-1]
PIRA31775.
RefSeqNP_001070145.1. NM_001076677.2. [P09496-3]
NP_001171689.1. NM_001184760.1. [P09496-4]
NP_001171691.1. NM_001184762.1. [P09496-5]
NP_001824.1. NM_001833.3. [P09496-2]
NP_009027.1. NM_007096.3. [P09496-1]
UniGeneHs.522114.

3D structure databases

ProteinModelPortalP09496.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid107621. 21 interactions.
IntActP09496. 21 interactions.
MINTMINT-1326541.
STRING9606.ENSP00000242285.

PTM databases

PhosphoSiteP09496.

Polymorphism databases

DMDM116501.

2D gel databases

OGPP09496.

Proteomic databases

MaxQBP09496.
PaxDbP09496.
PRIDEP09496.

Protocols and materials databases

DNASU1211.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENST00000242285; ENSP00000242285; ENSG00000122705. [P09496-1]
ENST00000345519; ENSP00000242284; ENSG00000122705. [P09496-2]
ENST00000396603; ENSP00000379848; ENSG00000122705. [P09496-3]
ENST00000433436; ENSP00000401019; ENSG00000122705. [P09496-1]
ENST00000470744; ENSP00000419746; ENSG00000122705. [P09496-4]
ENST00000538225; ENSP00000442869; ENSG00000122705. [P09496-4]
ENST00000540080; ENSP00000437508; ENSG00000122705. [P09496-5]
GeneID1211.
KEGGhsa:1211.
UCSCuc003zzc.3. human. [P09496-1]
uc003zzd.3. human. [P09496-3]
uc003zze.3. human. [P09496-2]
uc011lpk.2. human. [P09496-4]

Organism-specific databases

CTD1211.
GeneCardsGC09P036180.
HGNCHGNC:2090. CLTA.
HPACAB002665.
HPA050918.
MIM118960. gene.
neXtProtNX_P09496.
PharmGKBPA26616.
GenAtlasSearch...

Phylogenomic databases

eggNOGNOG312397.
HOGENOMHOG000008147.
HOVERGENHBG003386.
InParanoidP09496.
KOK04644.
OMAMDDFDML.
OrthoDBEOG79KPH4.
PhylomeDBP09496.
TreeFamTF313162.

Enzyme and pathway databases

ReactomeREACT_111045. Developmental Biology.
REACT_111102. Signal Transduction.
REACT_11123. Membrane Trafficking.
REACT_116125. Disease.
REACT_6900. Immune System.

Gene expression databases

ArrayExpressP09496.
BgeeP09496.
CleanExHS_CLTA.
GenevestigatorP09496.

Family and domain databases

InterProIPR000996. Clathrin_L-chain.
[Graphical view]
PANTHERPTHR10639. PTHR10639. 1 hit.
PfamPF01086. Clathrin_lg_ch. 1 hit.
[Graphical view]
PROSITEPS00224. CLATHRIN_LIGHT_CHN_1. 1 hit.
PS00581. CLATHRIN_LIGHT_CHN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSCLTA. human.
GenomeRNAi1211.
NextBio4985.
PMAP-CutDBP09496.
PROP09496.
SOURCESearch...

Entry information

Entry nameCLCA_HUMAN
AccessionPrimary (citable) accession number: P09496
Secondary accession number(s): A8K4W3 expand/collapse secondary AC list , B4DIN1, F5H6N3, Q2XPN5, Q53XZ1
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: July 9, 2014
This is version 140 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Relevant documents

SIMILARITY comments

Index of protein domains and families

MIM cross-references

Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot

Human chromosome 9

Human chromosome 9: entries, gene names and cross-references to MIM