P09496 (CLCA_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Clathrin light chain A
Short name=Lca

Gene names

Name:

CLTA

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	248 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Clathrin is the major protein of the polyhedral coat of coated pits and vesicles.
Subunit structure	Clathrin coats are formed from molecules containing 3 heavy chains and 3 light chains. Interacts with CALY; the interaction stimulates clathrin self-assembly and clathrin-mediated endocytosis. Ref.8
Subcellular location	Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Membrane › coated pit; Peripheral membrane protein; Cytoplasmic side. Note: Cytoplasmic face of coated pits and vesicles.
Sequence similarities	Belongs to the clathrin light chain family.

Ontologies

Keywords
Cellular component	Coated pit Cytoplasmic vesicle Membrane
Coding sequence diversity	Alternative splicing
Ligand	Calcium
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	axon guidance Traceable author statement. Source: Reactome epidermal growth factor receptor signaling pathway Traceable author statement. Source: Reactome intracellular protein transport Non-traceable author statement. Source: UniProtKB negative regulation of epidermal growth factor receptor signaling pathway Traceable author statement. Source: Reactome nerve growth factor receptor signaling pathway Traceable author statement. Source: Reactome post-Golgi vesicle-mediated transport Traceable author statement. Source: Reactome
Cellular component	clathrin coat of coated pit Inferred from electronic annotation. Source: InterPro clathrin coat of trans-Golgi network vesicle Inferred from electronic annotation. Source: InterPro cytosol Traceable author statement. Source: Reactome
Molecular function	structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform Brain (identifier: P09496-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Non-brain (identifier: P09496-2)

The sequence of this isoform differs from the canonical sequence as follows:
     163-192: Missing.

Isoform 3 (identifier: P09496-3)

The sequence of this isoform differs from the canonical sequence as follows:
     181-192: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 248	248	Clathrin light chain A		PRO_0000205767
Regions
	Region	100 – 162	63	Involved in binding clathrin heavy chain
Amino acid modifications
	Modified residue	105	1	Phosphoserine Ref.9
	Modified residue	206	1	Phosphoserine Ref.9 Ref.10 Ref.11
	Modified residue	236	1	Phosphoserine Ref.9 Ref.10
Natural variations
	Alternative sequence	163 – 192	30	Missing in isoform Non-brain.		VSP_001095
	Alternative sequence	181 – 192	12	Missing in isoform 3.		VSP_024238
Experimental info
	Sequence conflict	207	1	P → Q in ABB76683. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform Brain [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 8D8A3B49E6353D93	FASTA	248	27,077
	10 20 30 40 50 60 MAELDPFGAP AGAPGGPALG NGVAGAGEED PAAAFLAQQE SEIAGIENDE AFAILDGGAP 70 80 90 100 110 120 GPQPHGEPPG GPDAVDGVMN GEYYQESNGP TDSYAAISQV DRLQSEPESI RKWREEQMER 130 140 150 160 170 180 LEALDANSRK QEAEWKEKAI KELEEWYARQ DEQLQKTKAN NRVADEAFYK QPFADVIGYV 190 200 210 220 230 240 TNINHPCYSL EQAAEEAFVN DIDESSPGTE WERVARLCDF NPKSSKQAKD VSRMRSVLIS LKQAPLVH
	Isoform Non-brain [UniParc]. Checksum: 05C5037569FB6E8D	FASTA	218	23,662
	10 20 30 40 50 60 MAELDPFGAP AGAPGGPALG NGVAGAGEED PAAAFLAQQE SEIAGIENDE AFAILDGGAP 70 80 90 100 110 120 GPQPHGEPPG GPDAVDGVMN GEYYQESNGP TDSYAAISQV DRLQSEPESI RKWREEQMER 130 140 150 160 170 180 LEALDANSRK QEAEWKEKAI KELEEWYARQ DEQLQKTKAN NRAAEEAFVN DIDESSPGTE 190 200 210 WERVARLCDF NPKSSKQAKD VSRMRSVLIS LKQAPLVH
	Isoform 3 [UniParc]. Checksum: DAD1A01F3FD51284	FASTA	236	25,676
	10 20 30 40 50 60 MAELDPFGAP AGAPGGPALG NGVAGAGEED PAAAFLAQQE SEIAGIENDE AFAILDGGAP 70 80 90 100 110 120 GPQPHGEPPG GPDAVDGVMN GEYYQESNGP TDSYAAISQV DRLQSEPESI RKWREEQMER 130 140 150 160 170 180 LEALDANSRK QEAEWKEKAI KELEEWYARQ DEQLQKTKAN NRVADEAFYK QPFADVIGYV 190 200 210 220 230 AAEEAFVNDI DESSPGTEWE RVARLCDFNP KSSKQAKDVS RMRSVLISLK QAPLVH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure of human clathrin light chains. Conservation of light chain polymorphism in three mammalian species." Jackson A.P., Parham P. J. Biol. Chem. 263:16688-16695(1988) [PubMed: 3267234] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS BRAIN AND NON-BRAIN).
[2]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM NON-BRAIN).
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM NON-BRAIN).
[4]	Lin L., Nong W., Zhou G., Ke R., Shen C., Zhong G., Zheng Z., Liang M., Tang Z., Huang B., Li H., Yang S. Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
[5]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed: 15164053] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM NON-BRAIN). Tissue: Lung.
[8]	"Calcyon, a novel partner of clathrin light chain, stimulates clathrin-mediated endocytosis." Xiao J., Dai R., Negyessy L., Bergson C. J. Biol. Chem. 281:15182-15193(2006) [PubMed: 16595675] [Abstract] Cited for: INTERACTION WITH CALY.
[9]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-105; SER-206 AND SER-236, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[10]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-206 AND SER-236, MASS SPECTROMETRY. Tissue: Cervix carcinoma.
[11]	"Large-scale proteomics analysis of the human kinome." Oppermann F.S., Gnad F., Olsen J.V., Hornberger R., Greff Z., Keri G., Mann M., Daub H. Mol. Cell. Proteomics 8:1751-1764(2009) [PubMed: 19369195] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-206, MASS SPECTROMETRY.
[12]	"Initial characterization of the human central proteome." Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T., Bennett K.L., Superti-Furga G., Colinge J. BMC Syst. Biol. 5:17-17(2011) [PubMed: 21269460] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M20471 mRNA. Translation: AAA51817.1. M20472 mRNA. Translation: AAA59505.1. DQ270158 mRNA. Translation: ABB76683.1. BT007170 mRNA. Translation: AAP35834.1. AK291078 mRNA. Translation: BAF83767.1. AL158830 Genomic DNA. Translation: CAD13387.1. AL158830 Genomic DNA. Translation: CAD13388.1. CH471071 Genomic DNA. Translation: EAW58312.1. BC009201 mRNA. Translation: AAH09201.1. BC019287 mRNA. Translation: AAH19287.1.
IPI	IPI00014587. IPI00216393. IPI00790571.
PIR	A31775.
RefSeq	NP_001070145.1. NM_001076677.2. NP_001824.1. NM_001833.3. NP_009027.1. NM_007096.3.
UniGene	Hs.522114.
3D structure databases
ProteinModelPortal	P09496.
ModBase	Search...
Protein-protein interaction databases
IntAct	P09496. 13 interactions.
STRING	P09496.
PTM databases
PhosphoSite	P09496.
Polymorphism databases
DMDM	116501.
2D gel databases
OGP	P09496.
Proteomic databases
PRIDE	P09496.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000242285; ENSP00000242285; ENSG00000122705. ENST00000433436; ENSP00000401019; ENSG00000122705.
GeneID	1211.
KEGG	hsa:1211.
UCSC	uc003zzc.1. human. uc003zzd.1. human. uc003zze.1. human.
Organism-specific databases
CTD	1211.
GeneCards	GC09P036180.
H-InvDB	HIX0008030.
HGNC	HGNC:2090. CLTA.
HPA	CAB002665.
MIM	118960. gene.
neXtProt	NX_P09496.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG05521.
GeneTree	ENSGT00390000010441.
HOGENOM	HBG445728.
HOVERGEN	HBG003386.
InParanoid	P09496.
OMA	DEGFSIL.
OrthoDB	EOG4TF0M2.
PhylomeDB	P09496.
Enzyme and pathway databases
Pathway_Interaction_DB	arf_3pathway. Arf1 pathway.
Reactome	REACT_111045. Developmental Biology. REACT_111102. Signal Transduction. REACT_11123. Membrane Trafficking.
Gene expression databases
ArrayExpress	P09496.
Bgee	P09496.
CleanEx	HS_CLTA.
Genevestigator	P09496.
GermOnline	ENSG00000122705. Homo sapiens.
Family and domain databases
InterPro	IPR000996. Clathrin_L-chain. [Graphical view]
KO	K04644.
PANTHER	PTHR10639. Clathrin_lg_ch. 1 hit.
Pfam	PF01086. Clathrin_lg_ch. 1 hit. [Graphical view]
PROSITE	PS00224. CLATHRIN_LIGHT_CHN_1. 1 hit. PS00581. CLATHRIN_LIGHT_CHN_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	4985.
PMAP-CutDB	P09496.
SOURCE	Search...

Entry information

Entry name

CLCA_HUMAN

Accession

Primary (citable) accession number: P09496
Secondary accession number(s): A8K4W3, Q2XPN5, Q53XZ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	December 14, 2011
This is version 115 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.