Reviewed, UniProtKB/Swiss-Prot P09496 (CLCA_HUMAN)

Names and origin

Protein names

Recommended name:
    Clathrin light chain A
      Short name=Lca

Gene names

Name:

CLTA

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	248 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Clathrin is the major protein of the polyhedral coat of coated pits and vesicles.
Subunit structure	Clathrin coats are formed from molecules containing 3 heavy chains and 3 light chains. Interacts with CALY; the interaction stimulates clathrin self-assembly and clathrin-mediated endocytosis. Ref.8
Subcellular location	Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Membrane › coated pit; Peripheral membrane protein; Cytoplasmic side. Note: Cytoplasmic face of coated pits and vesicles.

Ontologies

Keywords
Cellular component	Coated pit Cytoplasmic vesicle Membrane
Coding sequence diversity	Alternative splicing
Ligand	Calcium
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	intracellular protein transport Non-traceable author statement. Source: UniProtKB membrane organization Inferred from Experiment. Source: Reactome post-Golgi vesicle-mediated transport Inferred from Experiment. Source: Reactome
Cellular component	clathrin coat of coated pit Inferred from electronic annotation. Source: InterPro clathrin coat of trans-Golgi network vesicle Inferred from electronic annotation. Source: InterPro internal side of plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: UniProtKB-KW protein binding Inferred from electronic annotation. Source: InterPro structural molecule activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform Brain (identifier: P09496-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Non-brain (identifier: P09496-2)

The sequence of this isoform differs from the canonical sequence as follows:
     163-192: Missing.

Isoform 3 (identifier: P09496-3)

The sequence of this isoform differs from the canonical sequence as follows:
     181-192: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 248	248	Clathrin light chain A		PRO_0000205767
Regions
	Region	100 – 162	63	Involved in binding clathrin heavy chain
Amino acid modifications
	Modified residue	105	1	Phosphoserine Ref.9
	Modified residue	206	1	Phosphoserine Ref.9 Ref.10
	Modified residue	236	1	Phosphoserine Ref.9 Ref.10
Natural variations
	Alternative sequence	163 – 192	30	Missing in isoform Non-brain.		VSP_001095
	Alternative sequence	181 – 192	12	Missing in isoform 3.		VSP_024238
Experimental info
	Sequence conflict	207	1	P → Q in ABB76683. Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Isoform Brain [UniParc]. Last modified July 1, 1989. Version 1. Checksum: 8D8A3B49E6353D93	FASTA	248	27,077
	10 20 30 40 50 60 MAELDPFGAP AGAPGGPALG NGVAGAGEED PAAAFLAQQE SEIAGIENDE AFAILDGGAP 70 80 90 100 110 120 GPQPHGEPPG GPDAVDGVMN GEYYQESNGP TDSYAAISQV DRLQSEPESI RKWREEQMER 130 140 150 160 170 180 LEALDANSRK QEAEWKEKAI KELEEWYARQ DEQLQKTKAN NRVADEAFYK QPFADVIGYV 190 200 210 220 230 240 TNINHPCYSL EQAAEEAFVN DIDESSPGTE WERVARLCDF NPKSSKQAKD VSRMRSVLIS LKQAPLVH
	Isoform Non-brain. Checksum: 05C5037569FB6E8D	FASTA	218	23,662
	10 20 30 40 50 60 MAELDPFGAP AGAPGGPALG NGVAGAGEED PAAAFLAQQE SEIAGIENDE AFAILDGGAP 70 80 90 100 110 120 GPQPHGEPPG GPDAVDGVMN GEYYQESNGP TDSYAAISQV DRLQSEPESI RKWREEQMER 130 140 150 160 170 180 LEALDANSRK QEAEWKEKAI KELEEWYARQ DEQLQKTKAN NRAAEEAFVN DIDESSPGTE 190 200 210 WERVARLCDF NPKSSKQAKD VSRMRSVLIS LKQAPLVH
	Isoform 3. Checksum: DAD1A01F3FD51284	FASTA	236	25,676
	10 20 30 40 50 60 MAELDPFGAP AGAPGGPALG NGVAGAGEED PAAAFLAQQE SEIAGIENDE AFAILDGGAP 70 80 90 100 110 120 GPQPHGEPPG GPDAVDGVMN GEYYQESNGP TDSYAAISQV DRLQSEPESI RKWREEQMER 130 140 150 160 170 180 LEALDANSRK QEAEWKEKAI KELEEWYARQ DEQLQKTKAN NRVADEAFYK QPFADVIGYV 190 200 210 220 230 AAEEAFVNDI DESSPGTEWE RVARLCDFNP KSSKQAKDVS RMRSVLISLK QAPLVH

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure of human clathrin light chains. Conservation of light chain polymorphism in three mammalian species." Jackson A.P., Parham P. J. Biol. Chem. 263:16688-16695(1988) [PubMed: 3267234] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS BRAIN AND NON-BRAIN).
[2]	"Cloning of human full-length CDSs in BD Creator(TM) system donor vector." Kalnine N., Chen X., Rolfs A., Halleck A., Hines L., Eisenstein S., Koundinya M., Raphael J., Moreira D., Kelley T., LaBaer J., Lin Y., Phelan M., Farmer A. Submitted (MAY-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM NON-BRAIN).
[3]	"Complete sequencing and characterization of 21,243 full-length human cDNAs." Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S. , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S. Nat. Genet. 36:40-45(2004) [PubMed: 14702039] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM NON-BRAIN).
[4]	Lin L., Nong W., Zhou G., Ke R., Shen C., Zhong G., Zheng Z., Liang M., Tang Z., Huang B., Li H., Yang S. Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 3).
[5]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed: 15164053] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[7]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM NON-BRAIN). Tissue: Lung.
[8]	"Calcyon, a novel partner of clathrin light chain, stimulates clathrin-mediated endocytosis." Xiao J., Dai R., Negyessy L., Bergson C. J. Biol. Chem. 281:15182-15193(2006) [PubMed: 16595675] [Abstract] Cited for: INTERACTION WITH CALY.
[9]	"Kinase-selective enrichment enables quantitative phosphoproteomics of the kinome across the cell cycle." Daub H., Olsen J.V., Bairlein M., Gnad F., Oppermann F.S., Korner R., Greff Z., Keri G., Stemmann O., Mann M. Mol. Cell 31:438-448(2008) [PubMed: 18691976] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-105; SER-206 AND SER-236, MASS SPECTROMETRY.
[10]	"A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-206 AND SER-236, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M20471 mRNA. Translation: AAA51817.1. M20472 mRNA. Translation: AAA59505.1. DQ270158 mRNA. Translation: ABB76683.1. BT007170 mRNA. Translation: AAP35834.1. AK291078 mRNA. Translation: BAF83767.1. AL158830 Genomic DNA. Translation: CAD13387.1. AL158830 Genomic DNA. Translation: CAD13388.1. CH471071 Genomic DNA. Translation: EAW58312.1. BC009201 mRNA. Translation: AAH09201.1. BC019287 mRNA. Translation: AAH19287.1.
IPI	IPI00014587. IPI00216393. IPI00790571.
PIR	A31775.
RefSeq	NP_001070145.1. NP_001824.1. NP_009027.1.
UniGene	Hs.522114
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P09496.
PTM databases
PhosphoSite	P09496.
2-D gel databases
OGP	P09496.
Proteomic databases
PRIDE	P09496.
Genome annotation databases
Ensembl	ENST00000242285; ENSP00000242285; ENSG00000122705; Homo sapiens. [Genome view] ENST00000345519; ENSP00000242284; ENSG00000122705; Homo sapiens. [Genome view] ENST00000396603; ENSP00000379848; ENSG00000122705; Homo sapiens. [Genome view] ENST00000433436; ENSP00000401019; ENSG00000122705; Homo sapiens. [Genome view]
GeneID	1211.
KEGG	hsa:1211.
UCSC	uc003zzc.1. human. uc003zzd.1. human. uc003zze.1. human.
Organism-specific databases
CTD	1211.
GeneCards	GC09P036180.
H-InvDB	HIX0008030.
HGNC	HGNC:2090. CLTA.
HPA	CAB002665.
MIM	118960. gene.
PharmGKB	PA26616.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	P09496.
HOVERGEN	P09496.
OMA	DEGFSIL.
Enzyme and pathway databases
Pathway_Interaction_DB	arf_3pathway. Arf1 pathway.
Reactome	REACT_11061. Signalling by NGF. REACT_11123. Membrane Trafficking. REACT_9417. Signaling by EGFR. REACT_9480. Gap junction trafficking and regulation.
Gene expression databases
ArrayExpress	P09496.
Bgee	P09496.
CleanEx	HS_CLTA.
Genevestigator	P09496.
GermOnline	ENSG00000122705. Homo sapiens.
Family and domain databases
InterPro	IPR000996. Clathrin_L-chain. [Graphical view]
PANTHER	PTHR10639. Clathrin_lg_ch. 1 hit.
Pfam	PF01086. Clathrin_lg_ch. 1 hit. [Graphical view]
PROSITE	PS00224. CLATHRIN_LIGHT_CHN_1. 1 hit. PS00581. CLATHRIN_LIGHT_CHN_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	4985.
PMAP-CutDB	P09496.
SOURCE	Search...

Entry information

Entry name

CLCA_HUMAN

Accession

Primary (citable) accession number: P09496
Secondary accession number(s): A8K4W3, Q2XPN5, Q53XZ1

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	July 1, 1989
Last modified:	November 3, 2009
This is version 93 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.