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P09487

- PPBT_BOVIN

UniProt

P09487 - PPBT_BOVIN

Protein

Alkaline phosphatase, tissue-nonspecific isozyme

Gene

ALPL

Organism
Bos taurus (Bovine)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 2 (01 May 2007)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotation

    Cofactori

    Binds 1 magnesium ion.By similarity
    Binds 2 zinc ions.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi60 – 601MagnesiumBy similarity
    Metal bindingi60 – 601Zinc 1By similarity
    Active sitei110 – 1101Phosphoserine intermediate
    Metal bindingi110 – 1101Zinc 1By similarity
    Metal bindingi173 – 1731MagnesiumBy similarity
    Metal bindingi332 – 3321MagnesiumBy similarity
    Metal bindingi337 – 3371Zinc 2By similarity
    Metal bindingi341 – 3411Zinc 2; via tele nitrogenBy similarity
    Metal bindingi378 – 3781Zinc 1By similarity
    Metal bindingi379 – 3791Zinc 1; via tele nitrogenBy similarity
    Metal bindingi454 – 4541Zinc 2; via tele nitrogenBy similarity

    GO - Molecular functioni

    1. alkaline phosphatase activity Source: MGI
    2. metal ion binding Source: UniProtKB-KW
    3. pyrophosphatase activity Source: Ensembl

    GO - Biological processi

    1. biomineral tissue development Source: AgBase
    2. cellular response to organic cyclic compound Source: Ensembl
    3. dephosphorylation Source: GOC
    4. developmental process involved in reproduction Source: Ensembl
    5. endochondral ossification Source: Ensembl
    6. response to antibiotic Source: Ensembl
    7. response to vitamin D Source: Ensembl

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Magnesium, Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Alkaline phosphatase, tissue-nonspecific isozyme (EC:3.1.3.1)
    Short name:
    AP-TNAP
    Short name:
    TNSALP
    Alternative name(s):
    Alkaline phosphatase liver/bone/kidney isozyme
    Gene namesi
    Name:ALPL
    OrganismiBos taurus (Bovine)
    Taxonomic identifieri9913 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
    ProteomesiUP000009136: Chromosome 2

    Subcellular locationi

    GO - Cellular componenti

    1. anchored component of membrane Source: UniProtKB-KW
    2. extracellular matrix Source: Ensembl
    3. extracellular membrane-bounded organelle Source: AgBase
    4. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 17171 PublicationAdd
    BLAST
    Chaini18 – 502485Alkaline phosphatase, tissue-nonspecific isozymePRO_0000024019Add
    BLAST
    Propeptidei503 – 52422Removed in mature formSequence AnalysisPRO_0000024020Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi139 ↔ 201By similarity
    Glycosylationi140 – 1401N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi230 – 2301N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi271 – 2711N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi303 – 3031N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi430 – 4301N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi489 ↔ 497By similarity
    Lipidationi502 – 5021GPI-anchor amidated serineSequence Analysis

    Keywords - PTMi

    Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein

    Proteomic databases

    PRIDEiP09487.

    Interactioni

    Subunit structurei

    Homodimer.

    Protein-protein interaction databases

    STRINGi9913.ENSBTAP00000011783.

    Structurei

    3D structure databases

    ProteinModelPortaliP09487.
    SMRiP09487. Positions 18-498.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the alkaline phosphatase family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG1785.
    GeneTreeiENSGT00390000008704.
    HOGENOMiHOG000099118.
    HOVERGENiHBG007345.
    InParanoidiP09487.
    KOiK01077.
    OMAiTWKSFKP.
    OrthoDBiEOG7SN8CH.
    TreeFamiTF323513.

    Family and domain databases

    Gene3Di3.40.720.10. 1 hit.
    InterProiIPR017849. Alkaline_Pase-like_a/b/a.
    IPR001952. Alkaline_phosphatase.
    IPR018299. Alkaline_phosphatase_AS.
    IPR017850. Alkaline_phosphatase_core.
    [Graphical view]
    PfamiPF00245. Alk_phosphatase. 1 hit.
    [Graphical view]
    PRINTSiPR00113. ALKPHPHTASE.
    SMARTiSM00098. alkPPc. 1 hit.
    [Graphical view]
    SUPFAMiSSF53649. SSF53649. 1 hit.
    PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P09487-1 [UniParc]FASTAAdd to Basket

    « Hide

    MISPFLLLAI GTCFASSLVP EKEKDPKYWR DQAQQTLKNA LRLQTLNTNV    50
    AKNVIMFLGD GMGVSTVTAA RILKGQLHHS PGEETKLEMD KFPYVALSKT 100
    YNTNAQVPDS AGTATAYLCG VKANEGTVGV SAATQRSQCN TTQGNEVTSI 150
    LRWAKDAGKS VGIVTTTRVN HATPSASYAH SADRDWYSDN EMPPEALSQG 200
    CKDIAYQLMH NIKDIEVIMG GGRKYMFPKN RTDVEYELDE KARGTRLDGL 250
    NLIDIWKSFK PKHKHSHYVW NRTDLLALDP HSVDYLLGLF EPGDMQYELN 300
    RNNATDPSLS EMVEMAIRIL NKNPKGFFLL VEGGRIDHGH HEGKAKQALH 350
    EAVEMDQAIG QAGAMTSVED TLTVVTADHS HVFTFGGYTP RGNSIFGLAP 400
    MVSDTDKKPF TAILYGNGPG YKVVGGEREN VSMVDYAHNN YQAQSAVPLR 450
    HETHGGEDVA VFAKGPMAHL LHGVHEQNYI PHVMAYAACI GANRDHCASA 500
    SSSGSPSPGP LLLLLALLPL GSLF 524
    Length:524
    Mass (Da):57,193
    Last modified:May 1, 2007 - v2
    Checksum:i21A66F10E8ADDFC2
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti210 – 2101H → Y in AAA30380. (PubMed:3436527)Curated
    Sequence conflicti475 – 4751H → Q in AAA30380. (PubMed:3436527)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M18443 mRNA. Translation: AAA30380.1.
    BC118208 mRNA. Translation: AAI18209.1.
    PIRiA29600.
    RefSeqiNP_789828.2. NM_176858.2.
    XP_005203122.1. XM_005203065.1.
    XP_005203123.1. XM_005203066.1.
    XP_005203124.1. XM_005203067.1.
    UniGeneiBt.13670.

    Genome annotation databases

    EnsembliENSBTAT00000011783; ENSBTAP00000011783; ENSBTAG00000008951.
    GeneIDi280994.
    KEGGibta:280994.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M18443 mRNA. Translation: AAA30380.1 .
    BC118208 mRNA. Translation: AAI18209.1 .
    PIRi A29600.
    RefSeqi NP_789828.2. NM_176858.2.
    XP_005203122.1. XM_005203065.1.
    XP_005203123.1. XM_005203066.1.
    XP_005203124.1. XM_005203067.1.
    UniGenei Bt.13670.

    3D structure databases

    ProteinModelPortali P09487.
    SMRi P09487. Positions 18-498.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 9913.ENSBTAP00000011783.

    Chemistry

    ChEMBLi CHEMBL2406894.

    Proteomic databases

    PRIDEi P09487.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSBTAT00000011783 ; ENSBTAP00000011783 ; ENSBTAG00000008951 .
    GeneIDi 280994.
    KEGGi bta:280994.

    Organism-specific databases

    CTDi 249.

    Phylogenomic databases

    eggNOGi COG1785.
    GeneTreei ENSGT00390000008704.
    HOGENOMi HOG000099118.
    HOVERGENi HBG007345.
    InParanoidi P09487.
    KOi K01077.
    OMAi TWKSFKP.
    OrthoDBi EOG7SN8CH.
    TreeFami TF323513.

    Miscellaneous databases

    NextBioi 20805094.

    Family and domain databases

    Gene3Di 3.40.720.10. 1 hit.
    InterProi IPR017849. Alkaline_Pase-like_a/b/a.
    IPR001952. Alkaline_phosphatase.
    IPR018299. Alkaline_phosphatase_AS.
    IPR017850. Alkaline_phosphatase_core.
    [Graphical view ]
    Pfami PF00245. Alk_phosphatase. 1 hit.
    [Graphical view ]
    PRINTSi PR00113. ALKPHPHTASE.
    SMARTi SM00098. alkPPc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53649. SSF53649. 1 hit.
    PROSITEi PS00123. ALKALINE_PHOSPHATASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and sequencing of bovine kidney alkaline phosphatase cDNA."
      Garattini E., Hua J.-C., Udenfriend S.
      Gene 59:41-46(1987) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. NIH - Mammalian Gene Collection (MGC) project
      Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: Hereford.
      Tissue: Thalamus.
    3. "Partial sequencing of human adult, human fetal, and bovine intestinal alkaline phosphatases: comparison with the human placental and liver isozymes."
      Hua J.-C., Berger J., Pan Y.C.E., Hulmes J.D., Udenfriend S.
      Proc. Natl. Acad. Sci. U.S.A. 83:2368-2372(1986) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 18-50.

    Entry informationi

    Entry nameiPPBT_BOVIN
    AccessioniPrimary (citable) accession number: P09487
    Secondary accession number(s): Q17QS5
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1989
    Last sequence update: May 1, 2007
    Last modified: October 1, 2014
    This is version 116 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney).

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3