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Reviewed, UniProtKB/Swiss-Prot P09487 (PPBT_BOVIN)

Last modified October 13, 2009. Version 79. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Alkaline phosphatase, tissue-nonspecific isozyme
    EC=3.1.3.1
Alternative name(s):
    AP-TNAP
    Liver/bone/kidney isozyme
Gene names
Name: ALPL
OrganismBos taurus (Bovine)
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

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Protein attributes

Sequence length524 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

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General annotation (Comments)

Catalytic activity

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactor

Binds 1 magnesium ion By similarity.

Binds 2 zinc ions By similarity.

Subunit structure

Homodimer.

Subcellular location

Cell membrane; Lipid-anchor › GPI-anchor.

Miscellaneous

In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney).

Sequence similarities

Belongs to the alkaline phosphatase family.

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Ontologies

Keywords
   Cellular componentCell membrane
Membrane
   DomainSignal
   LigandMagnesium
Metal-binding
Zinc
   Molecular functionHydrolase
   PTMGPI-anchor
Glycoprotein
Lipoprotein
Phosphoprotein
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological processmetabolic process

Inferred from electronic annotation. Source: InterPro

   Cellular componentanchored to membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

plasma membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionalkaline phosphatase activity

Inferred from electronic annotation. Source: EC

magnesium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

zinc ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1717 Ref.3
Chain18 – 502485Alkaline phosphatase, tissue-nonspecific isozyme
PRO_0000024019
Propeptide503 – 52422Removed in mature form Potential
PRO_0000024020

Sites

Active site1101Phosphoserine intermediate
Metal binding601Magnesium Potential
Metal binding601Zinc 2 Potential
Metal binding1731Magnesium Potential
Metal binding3321Magnesium Potential
Metal binding3371Zinc 1 Potential
Metal binding3411Zinc 1 Potential
Metal binding3781Zinc 2 Potential
Metal binding3791Zinc 2 Potential
Metal binding4541Zinc 1 Potential

Amino acid modifications

Lipidation5021GPI-anchor amidated serine Potential
Glycosylation1401N-linked (GlcNAc...) Potential
Glycosylation2301N-linked (GlcNAc...) Potential
Glycosylation2711N-linked (GlcNAc...) Potential
Glycosylation3031N-linked (GlcNAc...) Potential
Glycosylation4301N-linked (GlcNAc...) Potential

Experimental info

Sequence conflict2101H → Y in AAA30380. Ref.1
Sequence conflict4751H → Q in AAA30380. Ref.1

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Sequences

Sequence LengthMass (Da)Tools
P09487-1 [UniParc].

Last modified May 1, 2007. Version 2.
Checksum: 21A66F10E8ADDFC2

FASTA52457,193
        10         20         30         40         50         60 
MISPFLLLAI GTCFASSLVP EKEKDPKYWR DQAQQTLKNA LRLQTLNTNV AKNVIMFLGD 

        70         80         90        100        110        120 
GMGVSTVTAA RILKGQLHHS PGEETKLEMD KFPYVALSKT YNTNAQVPDS AGTATAYLCG 

       130        140        150        160        170        180 
VKANEGTVGV SAATQRSQCN TTQGNEVTSI LRWAKDAGKS VGIVTTTRVN HATPSASYAH 

       190        200        210        220        230        240 
SADRDWYSDN EMPPEALSQG CKDIAYQLMH NIKDIEVIMG GGRKYMFPKN RTDVEYELDE 

       250        260        270        280        290        300 
KARGTRLDGL NLIDIWKSFK PKHKHSHYVW NRTDLLALDP HSVDYLLGLF EPGDMQYELN 

       310        320        330        340        350        360 
RNNATDPSLS EMVEMAIRIL NKNPKGFFLL VEGGRIDHGH HEGKAKQALH EAVEMDQAIG 

       370        380        390        400        410        420 
QAGAMTSVED TLTVVTADHS HVFTFGGYTP RGNSIFGLAP MVSDTDKKPF TAILYGNGPG 

       430        440        450        460        470        480 
YKVVGGEREN VSMVDYAHNN YQAQSAVPLR HETHGGEDVA VFAKGPMAHL LHGVHEQNYI 

       490        500        510        520 
PHVMAYAACI GANRDHCASA SSSGSPSPGP LLLLLALLPL GSLF

« Hide

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References

« Hide 'large scale' references
[1]"Cloning and sequencing of bovine kidney alkaline phosphatase cDNA."
Garattini E., Hua J.-C., Udenfriend S.
Gene 59:41-46(1987) [PubMed: 3436527] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]NIH - Mammalian Gene Collection (MGC) project
Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Hereford.
Tissue: Thalamus.
[3]"Partial sequencing of human adult, human fetal, and bovine intestinal alkaline phosphatases: comparison with the human placental and liver isozymes."
Hua J.-C., Berger J., Pan Y.C.E., Hulmes J.D., Udenfriend S.
Proc. Natl. Acad. Sci. U.S.A. 83:2368-2372(1986) [PubMed: 3458202] [Abstract]
Cited for: PROTEIN SEQUENCE OF 18-50.

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Cross-references

Sequence databases

M18443 mRNA. Translation: AAA30380.1.
BC118208 mRNA. Translation: AAI18209.1.
IPIIPI00702768.
PIRA29600.
RefSeqNP_789828.2.
UniGeneBt.13670

3D structure databases

HSSPHSSP built from PDB template 1EW2 based on UniProtKB P05187.
ModBaseSearch...

Protein-protein interaction databases

STRINGP09487.

Genome annotation databases

EnsemblENSBTAT00000011783; ENSBTAP00000011783; ENSBTAG00000008951; Bos taurus. [Genome view]
GeneID280994.
KEGGbta:280994.

Organism-specific databases

CTD280994.

Phylogenomic databases

HOVERGENP09487.

Enzyme and pathway databases

BRENDA3.1.3.1. 251.

Family and domain databases

InterProIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
[Graphical view]
Gene3DG3DSA:3.40.720.10. Alk_phosphtse. 1 hit.
PfamPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSPR00113. ALKPHPHTASE.
SMARTSM00098. alkPPc. 1 hit.
[Graphical view]
PROSITEPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry namePPBT_BOVIN
AccessionPrimary (citable) accession number: P09487
Secondary accession number(s): Q17QS5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: May 1, 2007
Last modified: October 13, 2009
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHPI (Human Proteome Initiative)

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Relevant documents

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents