Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P09487 (PPBT_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 112. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Alkaline phosphatase, tissue-nonspecific isozyme

Short name=AP-TNAP
Short name=TNSALP
EC=3.1.3.1
Alternative name(s):
Alkaline phosphatase liver/bone/kidney isozyme
Gene names
Name:ALPL
OrganismBos taurus (Bovine) [Reference proteome]
Taxonomic identifier9913 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos

Protein attributes

Sequence length524 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

A phosphate monoester + H2O = an alcohol + phosphate.

Cofactor

Binds 1 magnesium ion By similarity.

Binds 2 zinc ions By similarity.

Subunit structure

Homodimer.

Subcellular location

Cell membrane; Lipid-anchorGPI-anchor.

Miscellaneous

In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney).

Sequence similarities

Belongs to the alkaline phosphatase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1717 Ref.3
Chain18 – 502485Alkaline phosphatase, tissue-nonspecific isozyme
PRO_0000024019
Propeptide503 – 52422Removed in mature form Potential
PRO_0000024020

Sites

Active site1101Phosphoserine intermediate
Metal binding601Magnesium By similarity
Metal binding601Zinc 1 By similarity
Metal binding1101Zinc 1 By similarity
Metal binding1731Magnesium By similarity
Metal binding3321Magnesium By similarity
Metal binding3371Zinc 2 By similarity
Metal binding3411Zinc 2; via tele nitrogen By similarity
Metal binding3781Zinc 1 By similarity
Metal binding3791Zinc 1; via tele nitrogen By similarity
Metal binding4541Zinc 2; via tele nitrogen By similarity

Amino acid modifications

Lipidation5021GPI-anchor amidated serine Potential
Glycosylation1401N-linked (GlcNAc...) Potential
Glycosylation2301N-linked (GlcNAc...) Potential
Glycosylation2711N-linked (GlcNAc...) Potential
Glycosylation3031N-linked (GlcNAc...) Potential
Glycosylation4301N-linked (GlcNAc...) Potential
Disulfide bond139 ↔ 201 By similarity
Disulfide bond489 ↔ 497 By similarity

Experimental info

Sequence conflict2101H → Y in AAA30380. Ref.1
Sequence conflict4751H → Q in AAA30380. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P09487 [UniParc].

Last modified May 1, 2007. Version 2.
Checksum: 21A66F10E8ADDFC2

FASTA52457,193
        10         20         30         40         50         60 
MISPFLLLAI GTCFASSLVP EKEKDPKYWR DQAQQTLKNA LRLQTLNTNV AKNVIMFLGD 

        70         80         90        100        110        120 
GMGVSTVTAA RILKGQLHHS PGEETKLEMD KFPYVALSKT YNTNAQVPDS AGTATAYLCG 

       130        140        150        160        170        180 
VKANEGTVGV SAATQRSQCN TTQGNEVTSI LRWAKDAGKS VGIVTTTRVN HATPSASYAH 

       190        200        210        220        230        240 
SADRDWYSDN EMPPEALSQG CKDIAYQLMH NIKDIEVIMG GGRKYMFPKN RTDVEYELDE 

       250        260        270        280        290        300 
KARGTRLDGL NLIDIWKSFK PKHKHSHYVW NRTDLLALDP HSVDYLLGLF EPGDMQYELN 

       310        320        330        340        350        360 
RNNATDPSLS EMVEMAIRIL NKNPKGFFLL VEGGRIDHGH HEGKAKQALH EAVEMDQAIG 

       370        380        390        400        410        420 
QAGAMTSVED TLTVVTADHS HVFTFGGYTP RGNSIFGLAP MVSDTDKKPF TAILYGNGPG 

       430        440        450        460        470        480 
YKVVGGEREN VSMVDYAHNN YQAQSAVPLR HETHGGEDVA VFAKGPMAHL LHGVHEQNYI 

       490        500        510        520 
PHVMAYAACI GANRDHCASA SSSGSPSPGP LLLLLALLPL GSLF 

« Hide

References

« Hide 'large scale' references
[1]"Cloning and sequencing of bovine kidney alkaline phosphatase cDNA."
Garattini E., Hua J.-C., Udenfriend S.
Gene 59:41-46(1987) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]NIH - Mammalian Gene Collection (MGC) project
Submitted (JUN-2006) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: Hereford.
Tissue: Thalamus.
[3]"Partial sequencing of human adult, human fetal, and bovine intestinal alkaline phosphatases: comparison with the human placental and liver isozymes."
Hua J.-C., Berger J., Pan Y.C.E., Hulmes J.D., Udenfriend S.
Proc. Natl. Acad. Sci. U.S.A. 83:2368-2372(1986) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 18-50.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M18443 mRNA. Translation: AAA30380.1.
BC118208 mRNA. Translation: AAI18209.1.
PIRA29600.
RefSeqNP_789828.2. NM_176858.2.
XP_005203122.1. XM_005203065.1.
XP_005203123.1. XM_005203066.1.
XP_005203124.1. XM_005203067.1.
UniGeneBt.13670.

3D structure databases

ProteinModelPortalP09487.
SMRP09487. Positions 18-498.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING9913.ENSBTAP00000011783.

Proteomic databases

PRIDEP09487.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSBTAT00000011783; ENSBTAP00000011783; ENSBTAG00000008951.
GeneID280994.
KEGGbta:280994.

Organism-specific databases

CTD249.

Phylogenomic databases

eggNOGCOG1785.
GeneTreeENSGT00390000008704.
HOGENOMHOG000099118.
HOVERGENHBG007345.
InParanoidP09487.
KOK01077.
OMAMQEALVP.
OrthoDBEOG7SN8CH.
TreeFamTF323513.

Family and domain databases

Gene3D3.40.720.10. 1 hit.
InterProIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSPR00113. ALKPHPHTASE.
SMARTSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMSSF53649. SSF53649. 1 hit.
PROSITEPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio20805094.

Entry information

Entry namePPBT_BOVIN
AccessionPrimary (citable) accession number: P09487
Secondary accession number(s): Q17QS5
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: May 1, 2007
Last modified: April 16, 2014
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families