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Protein

Alkaline phosphatase, tissue-nonspecific isozyme

Gene

ALPL

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

This isozyme may play a role in skeletal mineralization.By similarity

Catalytic activityi

A phosphate monoester + H2O = an alcohol + phosphate.PROSITE-ProRule annotationBy similarity

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarityNote: Binds 1 Mg2+ ion.By similarity
  • Zn2+By similarityNote: Binds 2 Zn2+ ions.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi60MagnesiumBy similarity1
Metal bindingi60Zinc 1By similarity1
Active sitei110Phosphoserine intermediateBy similarity1
Metal bindingi110Zinc 1By similarity1
Metal bindingi173MagnesiumBy similarity1
Metal bindingi332MagnesiumBy similarity1
Metal bindingi337Zinc 2By similarity1
Metal bindingi341Zinc 2; via tele nitrogenBy similarity1
Metal bindingi378Zinc 1By similarity1
Metal bindingi379Zinc 1; via tele nitrogenBy similarity1
Metal bindingi454Zinc 2; via tele nitrogenBy similarity1

GO - Molecular functioni

  • alkaline phosphatase activity Source: MGI
  • metal ion binding Source: UniProtKB-KW
  • pyrophosphatase activity Source: Ensembl

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Magnesium, Metal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.1.3.1. 908.

Names & Taxonomyi

Protein namesi
Recommended name:
Alkaline phosphatase, tissue-nonspecific isozyme (EC:3.1.3.1By similarity)
Short name:
AP-TNAP
Short name:
TNSALP
Alternative name(s):
Alkaline phosphatase liver/bone/kidney isozyme
Gene namesi
Name:ALPL
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 2

Subcellular locationi

  • Cell membrane By similarity; Lipid-anchorGPI-anchor By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2406894.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 171 PublicationAdd BLAST17
ChainiPRO_000002401918 – 502Alkaline phosphatase, tissue-nonspecific isozymeAdd BLAST485
PropeptideiPRO_0000024020503 – 524Removed in mature formSequence analysisAdd BLAST22

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei110PhosphoserineBy similarity1
Disulfide bondi139 ↔ 201By similarity
Glycosylationi140N-linked (GlcNAc...)Sequence analysis1
Glycosylationi230N-linked (GlcNAc...)Sequence analysis1
Glycosylationi271N-linked (GlcNAc...)Sequence analysis1
Glycosylationi303N-linked (GlcNAc...)Sequence analysis1
Glycosylationi430N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi489 ↔ 497By similarity
Lipidationi502GPI-anchor amidated serineSequence analysis1

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein, Phosphoprotein

Proteomic databases

PaxDbiP09487.
PRIDEiP09487.

Expressioni

Gene expression databases

BgeeiENSBTAG00000008951.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000011783.

Chemistry databases

BindingDBiP09487.

Structurei

3D structure databases

ProteinModelPortaliP09487.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the alkaline phosphatase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiKOG4126. Eukaryota.
COG1785. LUCA.
GeneTreeiENSGT00390000008704.
HOGENOMiHOG000099118.
HOVERGENiHBG007345.
InParanoidiP09487.
KOiK01077.
OMAiMDRAIGK.
OrthoDBiEOG091G067H.
TreeFamiTF323513.

Family and domain databases

CDDicd00016. alkPPc. 1 hit.
Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09487-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MISPFLLLAI GTCFASSLVP EKEKDPKYWR DQAQQTLKNA LRLQTLNTNV
60 70 80 90 100
AKNVIMFLGD GMGVSTVTAA RILKGQLHHS PGEETKLEMD KFPYVALSKT
110 120 130 140 150
YNTNAQVPDS AGTATAYLCG VKANEGTVGV SAATQRSQCN TTQGNEVTSI
160 170 180 190 200
LRWAKDAGKS VGIVTTTRVN HATPSASYAH SADRDWYSDN EMPPEALSQG
210 220 230 240 250
CKDIAYQLMH NIKDIEVIMG GGRKYMFPKN RTDVEYELDE KARGTRLDGL
260 270 280 290 300
NLIDIWKSFK PKHKHSHYVW NRTDLLALDP HSVDYLLGLF EPGDMQYELN
310 320 330 340 350
RNNATDPSLS EMVEMAIRIL NKNPKGFFLL VEGGRIDHGH HEGKAKQALH
360 370 380 390 400
EAVEMDQAIG QAGAMTSVED TLTVVTADHS HVFTFGGYTP RGNSIFGLAP
410 420 430 440 450
MVSDTDKKPF TAILYGNGPG YKVVGGEREN VSMVDYAHNN YQAQSAVPLR
460 470 480 490 500
HETHGGEDVA VFAKGPMAHL LHGVHEQNYI PHVMAYAACI GANRDHCASA
510 520
SSSGSPSPGP LLLLLALLPL GSLF
Length:524
Mass (Da):57,193
Last modified:May 1, 2007 - v2
Checksum:i21A66F10E8ADDFC2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti210H → Y in AAA30380 (PubMed:3436527).Curated1
Sequence conflicti475H → Q in AAA30380 (PubMed:3436527).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18443 mRNA. Translation: AAA30380.1.
BC118208 mRNA. Translation: AAI18209.1.
PIRiA29600.
RefSeqiNP_789828.2. NM_176858.2.
UniGeneiBt.13670.

Genome annotation databases

EnsembliENSBTAT00000011783; ENSBTAP00000011783; ENSBTAG00000008951.
GeneIDi280994.
KEGGibta:280994.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M18443 mRNA. Translation: AAA30380.1.
BC118208 mRNA. Translation: AAI18209.1.
PIRiA29600.
RefSeqiNP_789828.2. NM_176858.2.
UniGeneiBt.13670.

3D structure databases

ProteinModelPortaliP09487.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000011783.

Chemistry databases

BindingDBiP09487.
ChEMBLiCHEMBL2406894.

Proteomic databases

PaxDbiP09487.
PRIDEiP09487.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000011783; ENSBTAP00000011783; ENSBTAG00000008951.
GeneIDi280994.
KEGGibta:280994.

Organism-specific databases

CTDi249.

Phylogenomic databases

eggNOGiKOG4126. Eukaryota.
COG1785. LUCA.
GeneTreeiENSGT00390000008704.
HOGENOMiHOG000099118.
HOVERGENiHBG007345.
InParanoidiP09487.
KOiK01077.
OMAiMDRAIGK.
OrthoDBiEOG091G067H.
TreeFamiTF323513.

Enzyme and pathway databases

BRENDAi3.1.3.1. 908.

Miscellaneous databases

PROiP09487.

Gene expression databases

BgeeiENSBTAG00000008951.

Family and domain databases

CDDicd00016. alkPPc. 1 hit.
Gene3Di3.40.720.10. 1 hit.
InterProiIPR017849. Alkaline_Pase-like_a/b/a.
IPR001952. Alkaline_phosphatase.
IPR018299. Alkaline_phosphatase_AS.
IPR017850. Alkaline_phosphatase_core.
[Graphical view]
PfamiPF00245. Alk_phosphatase. 1 hit.
[Graphical view]
PRINTSiPR00113. ALKPHPHTASE.
SMARTiSM00098. alkPPc. 1 hit.
[Graphical view]
SUPFAMiSSF53649. SSF53649. 1 hit.
PROSITEiPS00123. ALKALINE_PHOSPHATASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPPBT_BOVIN
AccessioniPrimary (citable) accession number: P09487
Secondary accession number(s): Q17QS5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: May 1, 2007
Last modified: November 30, 2016
This is version 134 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney).

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.