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P09483 (ACHA4_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Neuronal acetylcholine receptor subunit alpha-4
Gene names
Name:Chrna4
Synonyms:Acra4
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length630 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

Subunit structure

Neuronal AChR is composed of two different types of subunits: alpha and beta. Alpha-4 subunit can be combined to beta-2 or beta-4 to give rise to functional receptors. Interacts with RIC3; which is required for proper folding and assembly By similarity.

Subcellular location

Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.

Tissue specificity

In various regions of the central nervous system.

Sequence similarities

Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-4/CHRNA4 sub-subfamily. [View classification]

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform Alpha-4-1 (identifier: P09483-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform Alpha-4-2 (identifier: P09483-2)

The sequence of this isoform differs from the canonical sequence as follows:
     629-630: AC → GMI

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 3030 Potential
Chain31 – 630600Neuronal acetylcholine receptor subunit alpha-4
PRO_0000000354

Regions

Topological domain32 – 249218Extracellular Potential
Transmembrane250 – 27021Helical; Potential
Transmembrane279 – 29921Helical; Potential
Transmembrane313 – 33321Helical; Potential
Topological domain334 – 604271Cytoplasmic Potential
Transmembrane605 – 62521Helical; Potential

Amino acid modifications

Glycosylation591N-linked (GlcNAc...) Potential
Glycosylation1091N-linked (GlcNAc...) Potential
Glycosylation1761N-linked (GlcNAc...) Potential
Disulfide bond163 ↔ 177 By similarity
Disulfide bond227 ↔ 228Associated with receptor activation By similarity

Natural variations

Alternative sequence629 – 6302AC → GMI in isoform Alpha-4-2.
VSP_000074

Experimental info

Sequence conflict1 – 44MANS → MEIGGPGA in AAA41676. Ref.1
Sequence conflict61T → P in AAB64439. Ref.3
Sequence conflict581A → G in AAA41676. Ref.1
Sequence conflict581A → G in AAB64439. Ref.3
Sequence conflict1961M → I in AAA41676. Ref.1
Sequence conflict1961M → I in AAB64439. Ref.3
Sequence conflict3011Missing in AAA41676. Ref.1
Sequence conflict3011Missing in AAC97071. Ref.1
Sequence conflict3011Missing in AAB64439. Ref.3

Sequences

Sequence LengthMass (Da)Tools
Isoform Alpha-4-1 [UniParc].

Last modified May 30, 2000. Version 3.
Checksum: 1DFFC9E1378E5417

FASTA63070,193
        10         20         30         40         50         60 
MANSGTGAPP PLLLLPLLLL LGTGLLPASS HIETRAHAEE RLLKRLFSGY NKWSRPVANI 

        70         80         90        100        110        120 
SDVVLVRFGL SIAQLIDVDE KNQMMTTNVW VKQEWHDYKL RWDPGDYENV TSIRIPSELI 

       130        140        150        160        170        180 
WRPDIVLYNN ADGDFAVTHL TKAHLFYDGR VQWTPPAIYK SSCSIDVTFF PFDQQNCTMK 

       190        200        210        220        230        240 
FGSWTYDKAK IDLVSMHSRV DQLDFWESGE WVIVDAVGTY NTRKYECCAE IYPDITYAFI 

       250        260        270        280        290        300 
IRRLPLFYTI NLIIPCLLIS CLTVLVFYLP SECGEKVTLC ISVLLSLTVF LLLITEIIPS 

       310        320        330        340        350        360 
PTSLVIPLIG EYLLFTMIFV TLSIVITVFV LNVHHRSPRT HTMPAWVRRV FLDIVPRLLF 

       370        380        390        400        410        420 
MKRPSVVKDN CRRLIESMHK MANAPRFWPE PVGEPGILSD ICNQGLSPAP TFCNPTDTAV 

       430        440        450        460        470        480 
ETQPTCRSPP LEVPDLKTSE VEKASPCPSP GSCPPPKSSS GAPMLIKARS LSVQHVPSSQ 

       490        500        510        520        530        540 
EAAEDGIRCR SRSIQYCVSQ DGAASLADSK PTSSPTSLKA RPSQLPVSDQ ASPCKCTCKE 

       550        560        570        580        590        600 
PSPVSPVTVL KAGGTKAPPQ HLPLSPALTR AVEGVQYIAD HLKAEDTDFS VKEDWKYVAM 

       610        620        630 
VIDRIFLWMF IIVCLLGTVG LFLPPWLAAC

« Hide

Isoform Alpha-4-2 [UniParc].

Checksum: 7F35DA69E1378E54
Show »

FASTA63170,320

References

[1]"Members of a nicotinic acetylcholine receptor gene family are expressed in different regions of the mammalian central nervous system."
Goldman D.J., Deneris E.S., Luyten W., Kochhar A., Patrick J., Heinemann S.F.
Cell 48:965-973(1987) [PubMed: 3829125] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM ALPHA-4-1), NUCLEOTIDE SEQUENCE [MRNA] OF 5-630 (ISOFORM ALPHA-4-2).
Strain: Sprague-Dawley.
Tissue: Hippocampus and Hypothalamus.
[2]Hartley M., Goldman D.J., Heinemann S.F.
Submitted (DEC-1998) to the EMBL/GenBank/DDBJ databases
Cited for: SEQUENCE REVISION.
[3]Boulter J., Deneris E.S., Evans K., Heinemann S.F.
Submitted (JUN-1997) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM ALPHA-4-2).
[4]"Neuronal nicotinic acetylcholine receptor beta-subunit is coded for by the cDNA clone alpha 4."
Whiting P., Esch F., Shimasaki S., Lindstrom J.
FEBS Lett. 219:459-463(1987) [PubMed: 3609304] [Abstract]
Cited for: PROTEIN SEQUENCE OF 31-47.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M15682 mRNA. Translation: AAA41676.1.
L31620 mRNA. Translation: AAC97071.1.
AF007212 mRNA. Translation: AAB64439.1.
IPIIPI00214860.
IPI00230863.
RefSeqNP_077330.1. NM_024354.1.
UniGeneRn.9697.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1OLEmodel-A/C38-245[»]
ProteinModelPortalP09483.
SMRP09483. Positions 245-337.
ModBaseSearch...

Protein-protein interaction databases

STRINGP09483.

PTM databases

PhosphoSiteP09483.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID25590.
KEGGrno:25590.

Organism-specific databases

CTD1137.
RGD2346. Chrna4.

Phylogenomic databases

eggNOGroNOG10402.
GeneTreeENSGT00560000076703.
HOVERGENHBG003756.
InParanoidP09483.
OrthoDBEOG480HWN.

Gene expression databases

ArrayExpressP09483.
GenevestigatorP09483.
GermOnlineENSRNOG00000011202. Rattus norvegicus.

Family and domain databases

InterProIPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
Gene3DG3DSA:2.70.170.10. Neur_chan_lig_bd. 1 hit.
KOK04806.
PANTHERPTHR18945. Neur_channel. 1 hit.
PfamPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMSSF90112. Neu_channel_TM. 1 hit.
SSF63712. Neur_chan_LBD. 1 hit.
TIGRFAMsTIGR00860. LIC. 1 hit.
PROSITEPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio607263.

Entry information

Entry nameACHA4_RAT
AccessionPrimary (citable) accession number: P09483
Secondary accession number(s): O35769
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: May 30, 2000
Last modified: January 25, 2012
This is version 116 of the entry and version 3 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents