Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Neuronal acetylcholine receptor subunit alpha-4

Gene

CHRNA4

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIon channel, Ligand-gated ion channel, Receptor
Biological processIon transport, Transport

Enzyme and pathway databases

ReactomeiR-GGA-629587. Highly sodium permeable acetylcholine nicotinic receptors.
R-GGA-629594. Highly calcium permeable postsynaptic nicotinic acetylcholine receptors.
R-GGA-629597. Highly calcium permeable nicotinic acetylcholine receptors.

Names & Taxonomyi

Protein namesi
Recommended name:
Neuronal acetylcholine receptor subunit alpha-4
Gene namesi
Name:CHRNA4
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini24 – 237ExtracellularAdd BLAST214
Transmembranei238 – 262HelicalAdd BLAST25
Transmembranei270 – 288HelicalAdd BLAST19
Transmembranei304 – 325HelicalAdd BLAST22
Topological domaini326 – 595CytoplasmicAdd BLAST270
Transmembranei596 – 614HelicalAdd BLAST19

GO - Cellular componenti

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi289E → K: Reduces channel conductance by 50%. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL5569.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Add BLAST23
ChainiPRO_000000035524 – 622Neuronal acetylcholine receptor subunit alpha-4Add BLAST599

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi52N-linked (GlcNAc...)Sequence analysis1
Glycosylationi102N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi156 ↔ 170By similarity
Disulfide bondi220 ↔ 221Associated with receptor activationBy similarity
Lipidationi266S-palmitoyl cysteineBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

PaxDbiP09482.

Expressioni

Gene expression databases

BgeeiENSGALG00000005801.

Interactioni

Subunit structurei

Neuronal AChR seems to be composed of two different type of subunits: alpha and non-alpha (also called beta). A functional receptor seems to consist of two alpha-chains and three non-alpha chains.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CHRNB2P094847EBI-10686088,EBI-10686072

Protein-protein interaction databases

IntActiP09482. 1 interactor.
STRINGi9031.ENSGALP00000009302.

Chemistry databases

BindingDBiP09482.

Structurei

3D structure databases

ProteinModelPortaliP09482.
SMRiP09482.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3645. Eukaryota.
ENOG410XQGR. LUCA.
HOGENOMiHOG000006756.
HOVERGENiHBG003756.
InParanoidiP09482.
KOiK04806.
OMAiCASIFPA.
OrthoDBiEOG091G0R20.
PhylomeDBiP09482.
TreeFamiTF315605.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiView protein in InterPro
IPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiView protein in Pfam
PF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
PRINTSiPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 2 hits.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiView protein in PROSITE
PS00236. NEUROTR_ION_CHANNEL. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09482-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGFLVSKGNL LLLLCASIFP AFGHVETRAH AEERLLKKLF SGYNKWSRPV
60 70 80 90 100
ANISDVVLVR FGLSIAQLID VDEKNQMMTT NVWVKQEWHD YKLRWDPQEY
110 120 130 140 150
ENVTSIRIPS ELIWRPDIVL YNNADGDFAV THLTKAHLFY DGRIKWMPPA
160 170 180 190 200
IYKSSCSIDV TFFPFDQQNC KMKFGSWTYD KAKIDLVSMH SHVDQLDYWE
210 220 230 240 250
SGEWVIINAV GNYNSKKYEC CTEIYPDITY SFIIRRLPLF YTINLIIPCL
260 270 280 290 300
LISCLTVLVF YLPSECGEKI TLCISVLLSL TVFLLLITEI IPSTSLVIPL
310 320 330 340 350
IGEYLLFTMI FVTLSIIITV FVLNVHHRSP RTHTMPDWVR RVFLDIVPRL
360 370 380 390 400
LFMKRPSTVK DNCKKLIESM HKLTNSPRLW SETDMEPNFT TSSSPSPQSN
410 420 430 440 450
EPSPTSSFCA HLEEPAKPMC KSPSGQYSML HPEPPQVTCS SPKPSCHPLS
460 470 480 490 500
DTQTTSISKG RSLSVQQMYS PNKTEEGSIR CRSRSIQYCY LQEDSSQTNG
510 520 530 540 550
HSSASPASQR CHLNEEQPQH KPHQCKCKCR KGEAAGTPTQ GSKSHSNKGE
560 570 580 590 600
HLVLMSPALK LAVEGVHYIA DHLRAEDADF SVKEDWKYVA MVIDRIFLWM
610 620
FIIVCLLGTV GLFLPPWLAG MI
Length:622
Mass (Da):70,838
Last modified:July 1, 1989 - v1
Checksum:i290B035893D6485A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07348
, X07349, X07350, X07351, X07352, X07399 Genomic DNA. Translation: CAA30285.1.
AJ250361 mRNA. Translation: CAB59626.1.
PIRiS00379. ACCH4N.
RefSeqiNP_990145.1. NM_204814.1.
UniGeneiGga.4440.

Genome annotation databases

GeneIDi395606.
KEGGigga:395606.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07348
, X07349, X07350, X07351, X07352, X07399 Genomic DNA. Translation: CAA30285.1.
AJ250361 mRNA. Translation: CAB59626.1.
PIRiS00379. ACCH4N.
RefSeqiNP_990145.1. NM_204814.1.
UniGeneiGga.4440.

3D structure databases

ProteinModelPortaliP09482.
SMRiP09482.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP09482. 1 interactor.
STRINGi9031.ENSGALP00000009302.

Chemistry databases

BindingDBiP09482.
ChEMBLiCHEMBL5569.

Proteomic databases

PaxDbiP09482.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi395606.
KEGGigga:395606.

Organism-specific databases

CTDi1137.

Phylogenomic databases

eggNOGiKOG3645. Eukaryota.
ENOG410XQGR. LUCA.
HOGENOMiHOG000006756.
HOVERGENiHBG003756.
InParanoidiP09482.
KOiK04806.
OMAiCASIFPA.
OrthoDBiEOG091G0R20.
PhylomeDBiP09482.
TreeFamiTF315605.

Enzyme and pathway databases

ReactomeiR-GGA-629587. Highly sodium permeable acetylcholine nicotinic receptors.
R-GGA-629594. Highly calcium permeable postsynaptic nicotinic acetylcholine receptors.
R-GGA-629597. Highly calcium permeable nicotinic acetylcholine receptors.

Miscellaneous databases

PROiP09482.

Gene expression databases

BgeeiENSGALG00000005801.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiView protein in InterPro
IPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiView protein in Pfam
PF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
PRINTSiPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 2 hits.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiView protein in PROSITE
PS00236. NEUROTR_ION_CHANNEL. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiACHA4_CHICK
AccessioniPrimary (citable) accession number: P09482
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: March 15, 2017
This is version 137 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.