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P09482 (ACHA4_CHICK) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 105. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Neuronal acetylcholine receptor subunit alpha-4
Gene names
Name:CHRNA4
OrganismGallus gallus (Chicken) [Reference proteome]
Taxonomic identifier9031 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Archosauria › Dinosauria › Saurischia › Theropoda › Coelurosauria › Aves › Neognathae › Galliformes › Phasianidae › Phasianinae › Gallus

Protein attributes

Sequence length622 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

Subunit structure

Neuronal AChR seems to be composed of two different type of subunits: alpha and non-alpha (also called beta). A functional receptor seems to consist of two alpha-chains and three non-alpha chains. Ref.2

Subcellular location

Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein. Cell membrane; Lipid-anchor By similarity.

Sequence similarities

Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-4/CHRNA4 sub-subfamily. [View classification]

Ontologies

Keywords
   Biological processIon transport
Transport
   Cellular componentCell junction
Cell membrane
Membrane
Postsynaptic cell membrane
Synapse
   DomainSignal
Transmembrane
Transmembrane helix
   Molecular functionIon channel
Ligand-gated ion channel
Receptor
   PTMDisulfide bond
Glycoprotein
Lipoprotein
Palmitate
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processB cell activation

Inferred from electronic annotation. Source: Compara

DNA repair

Inferred from electronic annotation. Source: Compara

behavioral response to nicotine

Inferred from electronic annotation. Source: Compara

calcium ion transport

Inferred from electronic annotation. Source: Compara

cognition

Inferred from electronic annotation. Source: Compara

locomotory behavior

Inferred from electronic annotation. Source: Compara

membrane depolarization

Inferred from electronic annotation. Source: Compara

regulation of action potential

Inferred from electronic annotation. Source: Compara

regulation of dopamine secretion

Inferred from electronic annotation. Source: Compara

regulation of inhibitory postsynaptic membrane potential

Inferred from electronic annotation. Source: Compara

response to hypoxia

Inferred from electronic annotation. Source: Compara

response to oxidative stress

Inferred from electronic annotation. Source: Compara

sensory perception of pain

Inferred from electronic annotation. Source: Compara

synaptic transmission, cholinergic

Inferred from electronic annotation. Source: Compara

   Cellular_componentacetylcholine-gated channel complex

Inferred from electronic annotation. Source: Compara

cell junction

Inferred from electronic annotation. Source: UniProtKB-KW

external side of plasma membrane

Inferred from electronic annotation. Source: Compara

postsynaptic membrane

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionacetylcholine receptor activity

Inferred from electronic annotation. Source: Compara

acetylcholine-activated cation-selective channel activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2323
Chain24 – 622599Neuronal acetylcholine receptor subunit alpha-4
PRO_0000000355

Regions

Topological domain24 – 237214Extracellular
Transmembrane238 – 26225Helical
Transmembrane270 – 28819Helical
Transmembrane304 – 32522Helical
Topological domain326 – 595270Cytoplasmic
Transmembrane596 – 61419Helical

Amino acid modifications

Lipidation2661S-palmitoyl cysteine By similarity
Glycosylation521N-linked (GlcNAc...) Potential
Glycosylation1021N-linked (GlcNAc...) Potential
Disulfide bond156 ↔ 170 By similarity
Disulfide bond220 ↔ 221Associated with receptor activation By similarity

Experimental info

Mutagenesis2891E → K: Reduces channel conductance by 50%. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P09482 [UniParc].

Last modified July 1, 1989. Version 1.
Checksum: 290B035893D6485A

FASTA62270,838
        10         20         30         40         50         60 
MGFLVSKGNL LLLLCASIFP AFGHVETRAH AEERLLKKLF SGYNKWSRPV ANISDVVLVR 

        70         80         90        100        110        120 
FGLSIAQLID VDEKNQMMTT NVWVKQEWHD YKLRWDPQEY ENVTSIRIPS ELIWRPDIVL 

       130        140        150        160        170        180 
YNNADGDFAV THLTKAHLFY DGRIKWMPPA IYKSSCSIDV TFFPFDQQNC KMKFGSWTYD 

       190        200        210        220        230        240 
KAKIDLVSMH SHVDQLDYWE SGEWVIINAV GNYNSKKYEC CTEIYPDITY SFIIRRLPLF 

       250        260        270        280        290        300 
YTINLIIPCL LISCLTVLVF YLPSECGEKI TLCISVLLSL TVFLLLITEI IPSTSLVIPL 

       310        320        330        340        350        360 
IGEYLLFTMI FVTLSIIITV FVLNVHHRSP RTHTMPDWVR RVFLDIVPRL LFMKRPSTVK 

       370        380        390        400        410        420 
DNCKKLIESM HKLTNSPRLW SETDMEPNFT TSSSPSPQSN EPSPTSSFCA HLEEPAKPMC 

       430        440        450        460        470        480 
KSPSGQYSML HPEPPQVTCS SPKPSCHPLS DTQTTSISKG RSLSVQQMYS PNKTEEGSIR 

       490        500        510        520        530        540 
CRSRSIQYCY LQEDSSQTNG HSSASPASQR CHLNEEQPQH KPHQCKCKCR KGEAAGTPTQ 

       550        560        570        580        590        600 
GSKSHSNKGE HLVLMSPALK LAVEGVHYIA DHLRAEDADF SVKEDWKYVA MVIDRIFLWM 

       610        620 
FIIVCLLGTV GLFLPPWLAG MI

« Hide

References

[1]"Genes expressed in the brain define three distinct neuronal nicotinic acetylcholine receptors."
Nef P., Oneyser C., Alliod C., Couturier S., Ballivet M.
EMBO J. 7:595-601(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
Strain: White leghorn.
Tissue: Brain.
[2]"Pentameric structure and subunit stoichiometry of a neuronal nicotinic acetylcholine receptor."
Cooper E., Couturier S., Ballivet M.
Nature 350:235-238(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: MUTAGENESIS OF GLU-289, SUBUNIT.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X07348 expand/collapse EMBL AC list , X07349, X07350, X07351, X07352, X07399 Genomic DNA. Translation: CAA30285.1.
AJ250361 mRNA. Translation: CAB59626.1.
IPIIPI00575000.
PIRACCH4N. S00379.
RefSeqNP_990145.1. NM_204814.1.
UniGeneGga.4440.

3D structure databases

ProteinModelPortalP09482.
SMRP09482. Positions 238-329.
ModBaseSearch...

Protein-protein interaction databases

STRING9031.ENSGALP00000009302.

Proteomic databases

PaxDbP09482.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSGALT00000009316; ENSGALP00000009302; ENSGALG00000005801.
GeneID395606.
KEGGgga:395606.

Organism-specific databases

CTD1137.

Phylogenomic databases

eggNOGNOG290206.
GeneTreeENSGT00620000087732.
HOGENOMHOG000006756.
HOVERGENHBG003756.
InParanoidP09482.
KOK04806.
OMAEDGVRCR.
OrthoDBEOG480HWN.

Family and domain databases

Gene3D2.70.170.10. 1 hit.
InterProIPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERPTHR18945. PTHR18945. 1 hit.
PfamPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMSSF90112. Neu_channel_TM. 1 hit.
SSF63712. Neur_chan_LBD. 1 hit.
TIGRFAMsTIGR00860. LIC. 1 hit.
PROSITEPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

BindingDBP09482.
ChEMBLCHEMBL5569.
NextBio20815679.

Entry information

Entry nameACHA4_CHICK
AccessionPrimary (citable) accession number: P09482
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: April 3, 2013
This is version 105 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

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