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Protein

Neuronal acetylcholine receptor subunit alpha-4

Gene

CHRNA4

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIon channel, Ligand-gated ion channel, Receptor
Biological processIon transport, Transport

Enzyme and pathway databases

ReactomeiR-GGA-629587 Highly sodium permeable acetylcholine nicotinic receptors
R-GGA-629594 Highly calcium permeable postsynaptic nicotinic acetylcholine receptors
R-GGA-629597 Highly calcium permeable nicotinic acetylcholine receptors

Names & Taxonomyi

Protein namesi
Recommended name:
Neuronal acetylcholine receptor subunit alpha-4
Gene namesi
Name:CHRNA4
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
Proteomesi
  • UP000000539 Componenti: Unplaced

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini24 – 237ExtracellularAdd BLAST214
Transmembranei238 – 262HelicalAdd BLAST25
Transmembranei270 – 288HelicalAdd BLAST19
Transmembranei304 – 325HelicalAdd BLAST22
Topological domaini326 – 595CytoplasmicAdd BLAST270
Transmembranei596 – 614HelicalAdd BLAST19

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi289E → K: Reduces channel conductance by 50%. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL5569

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 23Add BLAST23
ChainiPRO_000000035524 – 622Neuronal acetylcholine receptor subunit alpha-4Add BLAST599

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi52N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi102N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi156 ↔ 170By similarity
Disulfide bondi220 ↔ 221Associated with receptor activationBy similarity
Lipidationi266S-palmitoyl cysteineBy similarity1

Keywords - PTMi

Disulfide bond, Glycoprotein, Lipoprotein, Palmitate

Proteomic databases

PaxDbiP09482
PRIDEiP09482

Expressioni

Gene expression databases

BgeeiENSGALG00000005801

Interactioni

Subunit structurei

Neuronal AChR seems to be composed of two different type of subunits: alpha and non-alpha (also called beta). A functional receptor seems to consist of two alpha-chains and three non-alpha chains.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
CHRNB2P094847EBI-10686088,EBI-10686072

Protein-protein interaction databases

IntActiP09482, 1 interactor
STRINGi9031.ENSGALP00000009302

Chemistry databases

BindingDBiP09482

Structurei

3D structure databases

ProteinModelPortaliP09482
SMRiP09482
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3645 Eukaryota
ENOG410XQGR LUCA
HOGENOMiHOG000006756
HOVERGENiHBG003756
InParanoidiP09482
KOiK04806
OMAiRTHTMPA
OrthoDBiEOG091G0R20
PhylomeDBiP09482
TreeFamiTF315605

Family and domain databases

Gene3Di2.70.170.10, 1 hit
InterProiView protein in InterPro
IPR006202 Neur_chan_lig-bd
IPR036734 Neur_chan_lig-bd_sf
IPR006201 Neur_channel
IPR036719 Neuro-gated_channel_TM_sf
IPR006029 Neurotrans-gated_channel_TM
IPR018000 Neurotransmitter_ion_chnl_CS
IPR002394 Nicotinic_acetylcholine_rcpt
PANTHERiPTHR18945 PTHR18945, 1 hit
PfamiView protein in Pfam
PF02931 Neur_chan_LBD, 1 hit
PF02932 Neur_chan_memb, 1 hit
PRINTSiPR00254 NICOTINICR
PR00252 NRIONCHANNEL
SUPFAMiSSF63712 SSF63712, 1 hit
SSF90112 SSF90112, 2 hits
TIGRFAMsiTIGR00860 LIC, 1 hit
PROSITEiView protein in PROSITE
PS00236 NEUROTR_ION_CHANNEL, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09482-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGFLVSKGNL LLLLCASIFP AFGHVETRAH AEERLLKKLF SGYNKWSRPV
60 70 80 90 100
ANISDVVLVR FGLSIAQLID VDEKNQMMTT NVWVKQEWHD YKLRWDPQEY
110 120 130 140 150
ENVTSIRIPS ELIWRPDIVL YNNADGDFAV THLTKAHLFY DGRIKWMPPA
160 170 180 190 200
IYKSSCSIDV TFFPFDQQNC KMKFGSWTYD KAKIDLVSMH SHVDQLDYWE
210 220 230 240 250
SGEWVIINAV GNYNSKKYEC CTEIYPDITY SFIIRRLPLF YTINLIIPCL
260 270 280 290 300
LISCLTVLVF YLPSECGEKI TLCISVLLSL TVFLLLITEI IPSTSLVIPL
310 320 330 340 350
IGEYLLFTMI FVTLSIIITV FVLNVHHRSP RTHTMPDWVR RVFLDIVPRL
360 370 380 390 400
LFMKRPSTVK DNCKKLIESM HKLTNSPRLW SETDMEPNFT TSSSPSPQSN
410 420 430 440 450
EPSPTSSFCA HLEEPAKPMC KSPSGQYSML HPEPPQVTCS SPKPSCHPLS
460 470 480 490 500
DTQTTSISKG RSLSVQQMYS PNKTEEGSIR CRSRSIQYCY LQEDSSQTNG
510 520 530 540 550
HSSASPASQR CHLNEEQPQH KPHQCKCKCR KGEAAGTPTQ GSKSHSNKGE
560 570 580 590 600
HLVLMSPALK LAVEGVHYIA DHLRAEDADF SVKEDWKYVA MVIDRIFLWM
610 620
FIIVCLLGTV GLFLPPWLAG MI
Length:622
Mass (Da):70,838
Last modified:July 1, 1989 - v1
Checksum:i290B035893D6485A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07348
, X07349, X07350, X07351, X07352, X07399 Genomic DNA Translation: CAA30285.1
AJ250361 mRNA Translation: CAB59626.1
PIRiS00379 ACCH4N
RefSeqiNP_990145.1, NM_204814.1
UniGeneiGga.4440

Genome annotation databases

GeneIDi395606
KEGGigga:395606

Similar proteinsi

Entry informationi

Entry nameiACHA4_CHICK
AccessioniPrimary (citable) accession number: P09482
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: May 23, 2018
This is version 145 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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