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Protein

Neuronal acetylcholine receptor subunit alpha-2

Gene

CHRNA2

Organism
Gallus gallus (Chicken)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Ion channel, Ligand-gated ion channel, Receptor

Keywords - Biological processi

Ion transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Neuronal acetylcholine receptor subunit alpha-2
Gene namesi
Name:CHRNA2
OrganismiGallus gallus (Chicken)
Taxonomic identifieri9031 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiArchelosauriaArchosauriaDinosauriaSaurischiaTheropodaCoelurosauriaAvesNeognathaeGalloanseraeGalliformesPhasianidaePhasianinaeGallus
ProteomesiUP000000539 Componenti: Unplaced

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini24 – 239216ExtracellularAdd
BLAST
Transmembranei240 – 26425HelicalAdd
BLAST
Transmembranei272 – 29019HelicalAdd
BLAST
Transmembranei306 – 32722HelicalAdd
BLAST
Topological domaini328 – 501174CytoplasmicAdd
BLAST
Transmembranei502 – 52019HelicalAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell junction, Cell membrane, Membrane, Postsynaptic cell membrane, Synapse

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2323Add
BLAST
Chaini24 – 528505Neuronal acetylcholine receptor subunit alpha-2PRO_0000000344Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi54 – 541N-linked (GlcNAc...)Sequence Analysis
Glycosylationi104 – 1041N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi158 ↔ 172By similarity
Disulfide bondi222 ↔ 223Associated with receptor activationBy similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Interactioni

Subunit structurei

Neuronal AChR seems to be composed of two different type of subunits: alpha and non-alpha (also called beta). A functional receptor seems to consist of two alpha-chains and three non-alpha chains.

Structurei

3D structure databases

ProteinModelPortaliP09480.
SMRiP09480. Positions 240-331.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi394 – 41017Poly-GluAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiNOG290206.
HOGENOMiHOG000006756.
HOVERGENiHBG003756.
InParanoidiP09480.
KOiK04804.
PhylomeDBiP09480.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 2 hits.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P09480-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGWPCRSIIP LLVWCFVTLQ AATREQKQPH GFAEDRLFKH LFTGYNRWSR
60 70 80 90 100
PVPNTSDVVI VKFGLSIAQL IDVDEKNQMM TTNVWLKQEW SDYKLRWNPE
110 120 130 140 150
DFDNVTSIRV PSEMIWIPDI VLYNNADGEF AVTHMTKAHL FSNGKVKWVP
160 170 180 190 200
PAIYKSSCSI DVTYFPFDQQ NCKMKFGSWT YDKAKIDLEN MEHHVDLKDY
210 220 230 240 250
WESGEWAIIN AIGRYNSKKY DCCTEIYPDI TFYFVIRRLP LFYTINLIIP
260 270 280 290 300
CLLISCLTVL VFYLPSDCGE KITLCISVLL SLTVFLLLIT EIIPSTSLVI
310 320 330 340 350
PLIGEYLLFT MIFVTLSIII TVFVLNVHHR SPSTHTMPHW VRSFFLGFIP
360 370 380 390 400
RWLFMKRPPL LLPAEGTTGQ YDPPGTRLST SRCWLETDVD DKWEEEEEEE
410 420 430 440 450
EEEEEEEEEE KAYPSRVPSG GSQGTQCHYS CERQAGKASG GPAPQVPLKG
460 470 480 490 500
EEVGSDQGLT LSPSILRALE GVQYIADHLR AEDADFSVKE DWKYVAMVID
510 520
RIFLWMFIIV CLLGTVGLFL PPYLAGMI
Length:528
Mass (Da):60,675
Last modified:July 1, 1989 - v1
Checksum:iE76C6360AF876364
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07339
, X07340, X07341, X07342, X07343, X07344 Genomic DNA. Translation: CAB59645.1.
AJ250360 mRNA. Translation: CAB59625.1.
PIRiS00377. ACCH2N.
RefSeqiNP_990146.1. NM_204815.2.
UniGeneiGga.337.

Genome annotation databases

GeneIDi395607.
KEGGigga:395607.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X07339
, X07340, X07341, X07342, X07343, X07344 Genomic DNA. Translation: CAB59645.1.
AJ250360 mRNA. Translation: CAB59625.1.
PIRiS00377. ACCH2N.
RefSeqiNP_990146.1. NM_204815.2.
UniGeneiGga.337.

3D structure databases

ProteinModelPortaliP09480.
SMRiP09480. Positions 240-331.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi395607.
KEGGigga:395607.

Organism-specific databases

CTDi1135.

Phylogenomic databases

eggNOGiNOG290206.
HOGENOMiHOG000006756.
HOVERGENiHBG003756.
InParanoidiP09480.
KOiK04804.
PhylomeDBiP09480.

Miscellaneous databases

NextBioi20815680.
PROiP09480.

Family and domain databases

Gene3Di1.20.120.370. 2 hits.
2.70.170.10. 1 hit.
InterProiIPR027361. Acetylcholine_rcpt_TM.
IPR006202. Neur_chan_lig-bd.
IPR006201. Neur_channel.
IPR006029. Neurotrans-gated_channel_TM.
IPR018000. Neurotransmitter_ion_chnl_CS.
IPR002394. Nicotinic_acetylcholine_rcpt.
[Graphical view]
PANTHERiPTHR18945. PTHR18945. 1 hit.
PfamiPF02931. Neur_chan_LBD. 1 hit.
PF02932. Neur_chan_memb. 1 hit.
[Graphical view]
PRINTSiPR00254. NICOTINICR.
PR00252. NRIONCHANNEL.
SUPFAMiSSF63712. SSF63712. 1 hit.
SSF90112. SSF90112. 2 hits.
TIGRFAMsiTIGR00860. LIC. 1 hit.
PROSITEiPS00236. NEUROTR_ION_CHANNEL. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Genes expressed in the brain define three distinct neuronal nicotinic acetylcholine receptors."
    Nef P., Oneyser C., Alliod C., Couturier S., Ballivet M.
    EMBO J. 7:595-601(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
    Strain: White leghorn.
    Tissue: Brain.

Entry informationi

Entry nameiACHA2_CHICK
AccessioniPrimary (citable) accession number: P09480
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: July 1, 1989
Last modified: July 22, 2015
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.