Reviewed, UniProtKB/Swiss-Prot P09478 (ACH1_DROME)

Names and origin

Protein names

Recommended name:
    Acetylcholine receptor subunit alpha-like 1

Gene names

Name:	nAcRalpha-96Aa
Synonyms:	Acr96Aa, AcrB, als
ORF Names:	CG5610

Organism

Drosophila melanogaster (Fruit fly) [Complete proteome]

Taxonomic identifier

7227 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Arthropoda › Hexapoda › Insecta › Pterygota › Neoptera › Endopterygota › Diptera › Brachycera › Muscomorpha › Ephydroidea › Drosophilidae › Drosophila › Sophophora

Protein attributes

Sequence length	567 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.
Subcellular location	Cell junction › synapse › postsynaptic cell membrane; Multi-pass membrane protein. Cell membrane; Multi-pass membrane protein.
Tissue specificity	CNS in embryos. Ref.1
Developmental stage	Late embryonic, late pupal and second instar larvae stages. Ref.1
Sequence similarities	Belongs to the ligand-gated ionic channel (TC 1.A.9) family. [View classification]

Ontologies

Keywords
Biological process	Ion transport Transport
Cellular component	Cell junction Cell membrane Membrane Postsynaptic cell membrane Synapse
Coding sequence diversity	Polymorphism
Domain	Signal Transmembrane
Molecular function	Ionic channel Receptor
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	ion transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cell junction Inferred from electronic annotation. Source: UniProtKB-KW integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell postsynaptic membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	nicotinic acetylcholine-activated cation-selective channel activity Inferred from electronic annotation. Source: InterPro receptor activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 21	21
	Chain	22 – 567	546	Acetylcholine receptor subunit alpha-like 1		PRO_0000000298
Regions
	Topological domain	22 – 240	219	Extracellular
	Transmembrane	241 – 264	24
	Transmembrane	272 – 290	19
	Transmembrane	306 – 325	20
	Topological domain	326 – 513	188	Cytoplasmic
	Transmembrane	514 – 532	19
Amino acid modifications
	Glycosylation	45	1	N-linked (GlcNAc...) Potential
	Glycosylation	233	1	N-linked (GlcNAc...) Potential
	Disulfide bond	149 ↔ 163		By similarity
	Disulfide bond	222 ↔ 223		Associated with receptor activation By similarity
Natural variations
	Natural variant	538	1	Y → H
Experimental info
	Sequence conflict	108	1	L → H in CAA30172. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P09478-1 [UniParc]. Last modified May 9, 2003. Version 2. Checksum: 08E1F721FB2A92AC	FASTA	567	64,019
	10 20 30 40 50 60 MGSVLFAAVF IALHFATGGL ANPDAKRLYD DLLSNYNRLI RPVGNNSDRL TVKMGLRLSQ 70 80 90 100 110 120 LIDVNLKNQI MTTNVWVEQE WNDYKLKWNP DDYGGVDTLH VPSEHIWLPD IVLYNNADGN 130 140 150 160 170 180 YEVTIMTKAI LHHTGKVVWK PPAIYKSFCE IDVEYFPFDE QTCFMKFGSW TYDGYMVDLR 190 200 210 220 230 240 HLKQTADSDN IEVGIDLQDY YISVEWDIMR VPAVRNEKFY SCCEEPYLDI VFNLTLRRKT 250 260 270 280 290 300 LFYTVNLIIP CVGISFLSVL VFYLPSDSGE KISLCISILL SLTVFFLLLA EIIPPTSLTV 310 320 330 340 350 360 PLLGKYLLFT MMLVTLSVVV TIAVLNVNFR SPVTHRMAPW VQRLFIQILP KLLCIERPKK 370 380 390 400 410 420 EEPEEDQPPE VLTDVYHLPP DVDKFVNYDS KRFSGDYGIP ALPASHRFDL AAAGGISAHC 430 440 450 460 470 480 FAEPPLPSSL PLPGADDDLF SPSGLNGDIS PGCCPAAAAA AAADLSPTFE KPYAREMEKT 490 500 510 520 530 540 IEGSRFIAQH VKNKDKFESV EEDWKYVAMV LDRMFLWIFA IACVVGTALI ILQAPSLYDQ 550 560 SQPIDILYSK IAKKKFELLK MGSENTL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Conservation of neural nicotinic acetylcholine receptors from Drosophila to vertebrate central nervous systems." Bossy B., Ballivet M., Spierer P. EMBO J. 7:611-618(1988) [PubMed: 2840281] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY, DEVELOPMENTAL STAGE. Strain: Oregon-R.
[2]	"The genome sequence of Drosophila melanogaster." Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D. , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C. Science 287:2185-2195(2000) [PubMed: 10731132] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Berkeley.
[3]	"Annotation of the Drosophila melanogaster euchromatic genome: a systematic review." Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S., Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E., Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P., Bettencourt B.R., Celniker S.E., de Grey A.D.N.J. , Drysdale R.A., Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M., Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E. Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002) [PubMed: 12537572] [Abstract] Cited for: GENOME REANNOTATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X07194 mRNA. Translation: CAA30172.1. AE014297 Genomic DNA. Translation: AAF56301.2.
PIR	ACFFA1. S00381.
RefSeq	NP_524481.2.
UniGene	Dm.2364
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P09478.
Genome annotation databases
Ensembl	FBtr0084619; FBpp0084003; FBgn0000036; Drosophila melanogaster. [Genome view]
GeneID	42918.
KEGG	dme:Dmel_CG5610.
NMPDR	fig|7227.3.peg.14446.
Organism-specific databases
CTD	42918.
FlyBase	FBgn0000036. nAcRalpha-96Aa.
Phylogenomic databases
HOGENOM	P09478.
OMA	HYPELAP
OrthoDB	EOG9W6P2P
Enzyme and pathway databases
BioCyc	DMEL-XXX-02:DMEL-XXX-02-013188-MON.
Gene expression databases
GermOnline	CG5610. Drosophila melanogaster.
Family and domain databases
InterPro	IPR006029. Neu_channel_TM. IPR006202. Neur_chan_lig_bd. IPR006201. Neur_channel. IPR018000. Neurotransmitter_ion_chnl_CS. IPR002394. Nicotinic_acetylcholine_rcpt_N. [Graphical view]
Gene3D	G3DSA:2.70.170.10. Neur_chan_lig_bd. 1 hit.
PANTHER	PTHR18945. Neur_channel. 1 hit.
Pfam	PF02931. Neur_chan_LBD. 1 hit. PF02932. Neur_chan_memb. 1 hit. [Graphical view]
PRINTS	PR00254. NICOTINICR. PR00252. NRIONCHANNEL.
TIGRFAMs	TIGR00860. LIC. 1 hit.
PROSITE	PS00236. NEUROTR_ION_CHANNEL. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	831261.

Entry information

Entry name

ACH1_DROME

Accession

Primary (citable) accession number: P09478
Secondary accession number(s): Q9VC74

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	May 9, 2003
Last modified:	November 24, 2009
This is version 99 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Drosophila annotation project