P09471 (GNAO_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Guanine nucleotide-binding protein G(o) subunit alpha

Gene names

Name:

GNAO1

Organism

Homo sapiens (Human) [Reference proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Primates › Haplorrhini › Simiiformes › Catarrhini › Hominoidea › Hominidae › Homininae › Homo

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems. The G(o) protein function is not clear. Stimulated by RGS14.
Subunit structure	Interacts with RGS14 By similarity. G proteins are composed of 3 units; alpha, beta and gamma. The alpha chain contains the guanine nucleotide binding site.
Sequence similarities	Belongs to the G-alpha family. G(i/o/t/z) subfamily.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing
Ligand	GTP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Transducer
PTM	ADP-ribosylation Lipoprotein Myristate Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	adenylate cyclase-modulating G-protein coupled receptor signaling pathway Inferred from Biological aspect of Ancestor. Source: RefGenome aging Inferred from electronic annotation. Source: Compara dopamine receptor signaling pathway Inferred from Biological aspect of Ancestor. Source: RefGenome forebrain development Inferred from electronic annotation. Source: Compara locomotory behavior Inferred from electronic annotation. Source: Compara muscle contraction Traceable author statement PubMed 9050846. Source: ProtInc negative regulation of calcium ion transport Inferred from electronic annotation. Source: Compara neuron projection development Inferred from electronic annotation. Source: Compara positive regulation of GTPase activity Inferred from electronic annotation. Source: Compara regulation of heart contraction Inferred from electronic annotation. Source: Compara response to cytokine stimulus Inferred from electronic annotation. Source: Compara response to drug Inferred from electronic annotation. Source: Compara response to hydrogen peroxide Inferred from electronic annotation. Source: Compara response to morphine Inferred from electronic annotation. Source: Compara
Cellular_component	heterotrimeric G-protein complex Inferred from Biological aspect of Ancestor. Source: RefGenome neuron projection Inferred from electronic annotation. Source: Compara
Molecular_function	G-protein beta/gamma-subunit complex binding Inferred from Biological aspect of Ancestor. Source: RefGenome G-protein coupled serotonin receptor binding Inferred from Biological aspect of Ancestor. Source: RefGenome GTP binding Inferred from electronic annotation. Source: UniProtKB-KW GTPase activity Inferred from Biological aspect of Ancestor. Source: RefGenome metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW signal transducer activity Inferred from Biological aspect of Ancestor. Source: RefGenome
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform Alpha-1 (identifier: P09471-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform Alpha-2 (identifier: P09471-2)

The sequence of this isoform differs from the canonical sequence as follows:
     249-354: MLFDSICNNK...ANNLRGCGLY → KLFDSICNNK...AKNLRGCGLY

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 354	353	Guanine nucleotide-binding protein G(o) subunit alpha		PRO_0000203702
Regions
	Nucleotide binding	40 – 47	8	GTP By similarity
	Nucleotide binding	176 – 182	7	GTP By similarity
	Nucleotide binding	201 – 205	5	GTP By similarity
	Nucleotide binding	270 – 273	4	GTP By similarity
Sites
	Metal binding	47	1	Magnesium By similarity
	Metal binding	182	1	Magnesium By similarity
	Binding site	326	1	GTP; via amide nitrogen By similarity
Amino acid modifications
	Modified residue	179	1	ADP-ribosylarginine; by cholera toxin By similarity
	Modified residue	351	1	ADP-ribosylcysteine; by pertussis toxin By similarity
	Lipidation	2	1	N-myristoyl glycine By similarity
	Lipidation	3	1	S-palmitoyl cysteine By similarity
Natural variations
	Alternative sequence	249 – 354	106	MLFDS…GCGLY → KLFDSICNNKWFTDTSIILF LNKKDIFEEKIKKSPLTICF PEYTGPSAFTEAVAYIQAQY ESKNKSAHKEIYTHVTCATD TNNIQFVFDAVTDVIIAKNL RGCGLY in isoform Alpha-2.		VSP_031250
Experimental info
	Sequence conflict	16	1	S → G in AAA52584. Ref.2
	Sequence conflict	171	1	T → L in AAA52584. Ref.2
	Sequence conflict	218	1	D → E in AAA52584. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform Alpha-1 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: 4F182926A8106E3E	FASTA	354	40,051
	10 20 30 40 50 60 MGCTLSAEER AALERSKAIE KNLKEDGISA AKDVKLLLLG AGESGKSTIV KQMKIIHEDG 70 80 90 100 110 120 FSGEDVKQYK PVVYSNTIQS LAAIVRAMDT LGIEYGDKER KADAKMVCDV VSRMEDTEPF 130 140 150 160 170 180 SAELLSAMMR LWGDSGIQEC FNRSREYQLN DSAKYYLDSL DRIGAADYQP TEQDILRTRV 190 200 210 220 230 240 KTTGIVETHF TFKNLHFRLF DVGGQRSERK KWIHCFEDVT AIIFCVALSG YDQVLHEDET 250 260 270 280 290 300 TNRMHESLML FDSICNNKFF IDTSIILFLN KKDLFGEKIK KSPLTICFPE YTGPNTYEDA 310 320 330 340 350 AAYIQAQFES KNRSPNKEIY CHMTCATDTN NIQVVFDAVT DIIIANNLRG CGLY
	Isoform Alpha-2 [UniParc] [UniParc]. Checksum: 181B613AAF92F123	FASTA	354	40,087
	10 20 30 40 50 60 MGCTLSAEER AALERSKAIE KNLKEDGISA AKDVKLLLLG AGESGKSTIV KQMKIIHEDG 70 80 90 100 110 120 FSGEDVKQYK PVVYSNTIQS LAAIVRAMDT LGIEYGDKER KADAKMVCDV VSRMEDTEPF 130 140 150 160 170 180 SAELLSAMMR LWGDSGIQEC FNRSREYQLN DSAKYYLDSL DRIGAADYQP TEQDILRTRV 190 200 210 220 230 240 KTTGIVETHF TFKNLHFRLF DVGGQRSERK KWIHCFEDVT AIIFCVALSG YDQVLHEDET 250 260 270 280 290 300 TNRMHESLKL FDSICNNKWF TDTSIILFLN KKDIFEEKIK KSPLTICFPE YTGPSAFTEA 310 320 330 340 350 VAYIQAQYES KNKSAHKEIY THVTCATDTN NIQFVFDAVT DVIIAKNLRG CGLY

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure of the human gene and two rat cDNAs encoding the alpha chain of GTP-binding regulatory protein Go: two different mRNAs are generated by alternative splicing." Tsukamoto T., Toyama R., Itoh H., Kozasa T., Matsuoka M., Kaziro Y. Proc. Natl. Acad. Sci. U.S.A. 88:2974-2978(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], ALTERNATIVE SPLICING.
[2]	"Molecular cloning and DNA sequence analysis of the human guanine nucleotide-binding protein Go alpha." Lavu S., Clark J., Swarup R., Matshushima K., Paturu K., Moss J., Kung H.-F. Biochem. Biophys. Res. Commun. 150:811-815(1988) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA].
[3]	"cDNA clones of human proteins involved in signal transduction sequenced by the Guthrie cDNA resource center (www.cdna.org)." Puhl H.L. III, Ikeda S.R., Aronstam R.S. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS ALPHA-1 AND ALPHA-2). Tissue: Brain.
[4]	"Identification and expression of GO1 and 2 alpha-subunit transcripts in human myometrium in relation to pregnancy." Duc-Goiran P., Bourgeois C., Mignot T.M., Robert B., Tanguy G., Ferre F. Biol. Reprod. 60:1528-1535(1999) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 246-354 (ISOFORM ALPHA-2). Tissue: Myometrium.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M60164 , M60156, M60157, M60158, M60159, M60160, M60163 Genomic DNA. Translation: AAA52585.1. M60162 , M60156, M60157, M60158, M60159, M60160, M60161 Genomic DNA. Translation: AAA52586.1. M19182, M19184 Genomic DNA. Translation: AAA52584.1. AF493894 mRNA. Translation: AAM12608.1. AF493895 mRNA. Translation: AAM12609.1. Y18213 mRNA. Translation: CAB46639.1.
IPI	IPI00220281. IPI00398700.
PIR	RGHUO1. A40436. RGHUO2. B40436.
RefSeq	NP_066268.1. NM_020988.2. NP_620073.2. NM_138736.2.
UniGene	Hs.644524.
3D structure databases
ProteinModelPortal	P09471.
ModBase	Search...
Protein-protein interaction databases
IntAct	P09471. 2 interactions.
MINT	MINT-1203064.
STRING	9606.ENSP00000262493.
PTM databases
PhosphoSite	P09471.
Polymorphism databases
DMDM	232133.
Proteomic databases
PaxDb	P09471.
PeptideAtlas	P09471.
PRIDE	P09471.
Protocols and materials databases
DNASU	2775.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000262493; ENSP00000262493; ENSG00000087258. ENST00000262494; ENSP00000262494; ENSG00000087258.
GeneID	2775.
KEGG	hsa:2775.
UCSC	uc002eit.4. human. uc002eiu.4. human.
Organism-specific databases
CTD	2775.
GeneCards	GC16P056277.
HGNC	HGNC:4389. GNAO1.
HPA	CAB008387.
MIM	139311. gene.
neXtProt	NX_P09471.
PharmGKB	PA28771.
GenAtlas	Search...
Phylogenomic databases
eggNOG	NOG322962.
HOGENOM	HOG000038730.
HOVERGEN	HBG063184.
KO	K04534.
OMA	TMSQEER.
OrthoDB	EOG44F69B.
Enzyme and pathway databases
Pathway_Interaction_DB	endothelinpathway. Endothelins. hedgehog_glipathway. Hedgehog signaling events mediated by Gli proteins. lysophospholipid_pathway. LPA receptor mediated events. er_nongenomic_pathway. Plasma membrane estrogen receptor signaling. s1p_s1p1_pathway. S1P1 pathway. s1p_s1p2_pathway. S1P2 pathway. s1p_s1p3_pathway. S1P3 pathway. s1p_s1p4_pathway. S1P4 pathway. s1p_s1p5_pathway. S1P5 pathway. s1p_meta_pathway. Sphingosine 1-phosphate (S1P) pathway.
Reactome	REACT_111102. Signal Transduction. REACT_111217. Metabolism.
SignaLink	P09471.
Gene expression databases
ArrayExpress	P09471.
Bgee	P09471.
CleanEx	HS_GNAO1.
Genevestigator	P09471.
GermOnline	ENSG00000087258. Homo sapiens.
Family and domain databases
Gene3D	1.10.400.10. 1 hit.
InterPro	IPR001408. Gprotein_alpha_I. IPR001019. Gprotein_alpha_su. IPR011025. GproteinA_insert. IPR027417. P-loop_NTPase. [Graphical view]
PANTHER	PTHR10218. PTHR10218. 1 hit.
Pfam	PF00503. G-alpha. 1 hit. [Graphical view]
PRINTS	PR00318. GPROTEINA. PR00441. GPROTEINAI.
SMART	SM00275. G_alpha. 1 hit. [Graphical view]
SUPFAM	SSF52540. SSF52540. 1 hit. SSF47895. Transducn_insert. 1 hit.
ProtoNet	Search...
Other
BindingDB	P09471.
ChEMBL	CHEMBL4742.
ChiTaRS	GNAO1. human.
GenomeRNAi	2775.
NextBio	10916.
SOURCE	Search...

Entry information

Entry name

GNAO_HUMAN

Accession

Primary (citable) accession number: P09471
Secondary accession number(s): P29777, Q8TD72, Q9UMV4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	January 23, 2007
Last modified:	May 29, 2013
This is version 141 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.