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Protein

Angiotensin-converting enzyme

Gene

Ace

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. This GPIase activity seems to be crucial for the egg-binding ability of the sperm.3 Publications

Catalytic activityi

Release of a C-terminal dipeptide, oligopeptide-|-Xaa-Yaa, when Xaa is not Pro, and Yaa is neither Asp nor Glu. Thus, conversion of angiotensin I to angiotensin II, with increase in vasoconstrictor activity, but no action on angiotensin II.

Cofactori

Protein has several cofactor binding sites:
  • Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit. Isoform Testis-specific only binds 1 Zn2+ ion per subunit.By similarity
  • chlorideBy similarityNote: Binds 3 chloride ions per subunit.By similarity

Enzyme regulationi

Peptidase activity is specifically inhibited by lisinopril, captopril and enalaprilat. In contrast, GPIase activity is nearly insensitive to captopril.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei236Chloride 1By similarity1
Metal bindingi395Zinc 1; catalyticBy similarity1
Active sitei3961PROSITE-ProRule annotation1
Metal bindingi399Zinc 1; catalyticBy similarity1
Metal bindingi423Zinc 1; catalyticBy similarity1
Binding sitei534Chloride 1By similarity1
Binding sitei796Chloride 2By similarity1
Binding sitei834Chloride 3By similarity1
Metal bindingi993Zinc 2; catalytic1
Active sitei99421
Metal bindingi997Zinc 2; catalytic1
Metal bindingi1021Zinc 2; catalyticBy similarity1
Binding sitei1095Chloride 2By similarity1
Binding sitei1099Chloride 2By similarity1
Binding sitei1132Chloride 3By similarity1

GO - Molecular functioni

GO - Biological processi

  • amyloid-beta metabolic process Source: MGI
  • angiogenesis involved in coronary vascular morphogenesis Source: MGI
  • animal organ regeneration Source: MGI
  • arachidonic acid secretion Source: MGI
  • bradykinin catabolic process Source: MGI
  • cell proliferation in bone marrow Source: BHF-UCL
  • eating behavior Source: MGI
  • embryo development ending in birth or egg hatching Source: MGI
  • heart contraction Source: MGI
  • hormone catabolic process Source: BHF-UCL
  • kidney development Source: MGI
  • lung alveolus development Source: MGI
  • negative regulation of blood vessel diameter Source: MGI
  • negative regulation of calcium ion import Source: MGI
  • negative regulation of gap junction assembly Source: BHF-UCL
  • negative regulation of gene expression Source: BHF-UCL
  • negative regulation of glucose import Source: MGI
  • negative regulation of protein binding Source: BHF-UCL
  • negative regulation of renal sodium excretion Source: MGI
  • neutrophil mediated immunity Source: MGI
  • peptide catabolic process Source: MGI
  • peptide metabolic process Source: MGI
  • positive regulation of apoptotic process Source: MGI
  • positive regulation of blood pressure Source: MGI
  • positive regulation of inflammatory response Source: MGI
  • positive regulation of neurogenesis Source: MGI
  • positive regulation of peptidyl-cysteine S-nitrosylation Source: BHF-UCL
  • positive regulation of peptidyl-tyrosine autophosphorylation Source: BHF-UCL
  • positive regulation of protein binding Source: BHF-UCL
  • positive regulation of protein tyrosine kinase activity Source: BHF-UCL
  • positive regulation of systemic arterial blood pressure Source: MGI
  • posttranscriptional regulation of gene expression Source: BHF-UCL
  • regulation of angiotensin metabolic process Source: MGI
  • regulation of blood pressure Source: BHF-UCL
  • regulation of hematopoietic stem cell proliferation Source: BHF-UCL
  • regulation of smooth muscle cell migration Source: MGI
  • regulation of systemic arterial blood pressure by renin-angiotensin Source: MGI
  • response to lipopolysaccharide Source: MGI
  • sensory perception of pain Source: MGI
  • spermatogenesis Source: MGI
  • vasoconstriction Source: MGI

Keywordsi

Molecular functionCarboxypeptidase, Hydrolase, Metalloprotease, Protease
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-MMU-2022377 Metabolism of Angiotensinogen to Angiotensins

Protein family/group databases

MEROPSiM02.001

Names & Taxonomyi

Protein namesi
Recommended name:
Angiotensin-converting enzyme (EC:3.2.1.-, EC:3.4.15.1)
Short name:
ACE
Alternative name(s):
Dipeptidyl carboxypeptidase I
Kininase II
CD_antigen: CD143
Cleaved into the following chain:
Gene namesi
Name:Ace
Synonyms:Dcp1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:87874 Ace

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini35 – 1264ExtracellularSequence analysisAdd BLAST1230
Transmembranei1265 – 1281HelicalSequence analysisAdd BLAST17
Topological domaini1282 – 1312CytoplasmicSequence analysisAdd BLAST31

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane, Secreted

Pathology & Biotechi

Disruption phenotypei

Low blood pressure, elevated serum potassium, anemia, and renal defects. Male mice have reduced fertility.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi993H → K: Abolishes peptidase activity but no effect on GPIase activity; when associated with K-997. 1 Publication1
Mutagenesisi994E → D: Abolishes peptidase activity but no effect on GPIase activity. 1 Publication1
Mutagenesisi997H → K: Abolishes peptidase activity but no effect on GPIase activity; when associated with K-993. 1 Publication1

Chemistry databases

ChEMBLiCHEMBL2994

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 341 PublicationAdd BLAST34
ChainiPRO_000002853935 – 1312Angiotensin-converting enzymeAdd BLAST1278
ChainiPRO_000002854035 – 1237Angiotensin-converting enzyme, soluble formAdd BLAST1203
PropeptideiPRO_00000285411238 – 1312Removed in secreted formBy similarityAdd BLAST75

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi59N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi79N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi116N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi151N-linked (GlcNAc...) asparagine1 Publication1
Disulfide bondi162 ↔ 170By similarity
Glycosylationi165N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi323N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi514N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi682N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi700N-linked (GlcNAc...) (complex) asparagineBy similarity1
Glycosylationi719N-linked (GlcNAc...) (complex) asparagineBy similarity1
Disulfide bondi762 ↔ 768By similarity
Glycosylationi765N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi947N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi962 ↔ 980By similarity
Disulfide bondi1148 ↔ 1160By similarity
Glycosylationi1196N-linked (GlcNAc...) asparagineSequence analysis1
Modified residuei1305PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by CK2 on Ser-1305; which allows membrane retention.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiP09470
PeptideAtlasiP09470
PRIDEiP09470

PTM databases

iPTMnetiP09470
PhosphoSitePlusiP09470

Expressioni

Tissue specificityi

Testis-specific isoform is expressed in spermatocytes, adult testis.

Inductioni

Expression is thought to be subject to hormonal regulation by androgens.

Gene expression databases

BgeeiENSMUSG00000020681
ExpressionAtlasiP09470 baseline and differential
GenevisibleiP09470 MM

Interactioni

GO - Molecular functioni

Protein-protein interaction databases

BioGridi197920, 1 interactor
IntActiP09470, 3 interactors
MINTiP09470
STRINGi10090.ENSMUSP00000001963

Chemistry databases

BindingDBiP09470

Structurei

3D structure databases

ProteinModelPortaliP09470
SMRiP09470
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni35 – 635Peptidase M2 1Add BLAST601
Regioni636 – 1237Peptidase M2 2Add BLAST602

Sequence similaritiesi

Belongs to the peptidase M2 family.Curated

Keywords - Domaini

Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3690 Eukaryota
ENOG410XPJ3 LUCA
GeneTreeiENSGT00520000055576
HOGENOMiHOG000007838
HOVERGENiHBG000264
InParanoidiP09470
KOiK01283
OMAiVTMEQLF
OrthoDBiEOG091G033S
PhylomeDBiP09470
TreeFamiTF312861

Family and domain databases

CDDicd06461 M2_ACE, 2 hits
InterProiView protein in InterPro
IPR001548 Peptidase_M2
PANTHERiPTHR10514 PTHR10514, 2 hits
PfamiView protein in Pfam
PF01401 Peptidase_M2, 2 hits
PRINTSiPR00791 PEPDIPTASEA
PROSITEiView protein in PROSITE
PS00142 ZINC_PROTEASE, 2 hits

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform Somatic (identifier: P09470-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MGAASGQRGR WPLSPPLLML SLLVLLLQPS PAPALDPGLQ PGNFSPDEAG
60 70 80 90 100
AQLFAESYNS SAEVVMFQST VASWAHDTNI TEENARRQEE AALVSQEFAE
110 120 130 140 150
VWGKKAKELY ESIWQNFTDS KLRRIIGSIR TLGPANLPLA QRQQYNSLLS
160 170 180 190 200
NMSRIYSTGK VCFPNKTATC WSLDPELTNI LASSRSYAKL LFAWEGWHDA
210 220 230 240 250
VGIPLKPLYQ DFTAISNEAY RQDDFSDTGA FWRSWYESPS FEESLEHIYH
260 270 280 290 300
QLEPLYLNLH AYVRRALHRR YGDKYVNLRG PIPAHLLGDM WAQSWENIYD
310 320 330 340 350
MVVPFPDKPN LDVTSTMVQK GWNATHMFRV SEEFFTSLGL SPMPPEFWAE
360 370 380 390 400
SMLEKPTDGR EVVCHASAWD FYNRKDFRIK QCTRVTMEQL ATVHHEMGHV
410 420 430 440 450
QYYLQYKDLH VSLRRGANPG FHEAIGDVLA LSVSTPAHLH KIGLLDHVTN
460 470 480 490 500
DIESDINYLL KMALEKIAFL PFGYLVDQWR WGVFSGRTPP SRYNFDWWYL
510 520 530 540 550
RTKYQGICPP VARNETHFDA GAKFHIPNVT PYIRYFVSFV LQFQFHQALC
560 570 580 590 600
KEAGHQGPLH QCDIYQSAQA GAKLKQVLQA GCSRPWQEVL KDLVGSDALD
610 620 630 640 650
AKALLEYFQP VSQWLEEQNQ RNGEVLGWPE NQWRPPLPDN YPEGIDLETD
660 670 680 690 700
EAKADRFVEE YDRTAQVLLN EYAEANWQYN TNITIEGSKI LLEKSTEVSN
710 720 730 740 750
HTLKYGTRAK TFDVSNFQNS SIKRIIKKLQ NLDRAVLPPK ELEEYNQILL
760 770 780 790 800
DMETTYSLSN ICYTNGTCMP LEPDLTNMMA TSRKYEELLW AWKSWRDKVG
810 820 830 840 850
RAILPFFPKY VEFSNKIAKL NGYTDAGDSW RSLYESDNLE QDLEKLYQEL
860 870 880 890 900
QPLYLNLHAY VRRSLHRHYG SEYINLDGPI PAHLLGNMWA QTWSNIYDLV
910 920 930 940 950
APFPSAPNID ATEAMIKQGW TPRRIFKEAD NFFTSLGLLP VPPEFWNKSM
960 970 980 990 1000
LEKPTDGREV VCHPSAWDFY NGKDFRIKQC TSVNMEDLVI AHHEMGHIQY
1010 1020 1030 1040 1050
FMQYKDLPVT FREGANPGFH EAIGDIMALS VSTPKHLYSL NLLSTEGSGY
1060 1070 1080 1090 1100
EYDINFLMKM ALDKIAFIPF SYLIDQWRWR VFDGSITKEN YNQEWWSLRL
1110 1120 1130 1140 1150
KYQGLCPPVP RSQGDFDPGS KFHVPANVPY VRYFVSFIIQ FQFHEALCRA
1160 1170 1180 1190 1200
AGHTGPLHKC DIYQSKEAGK LLADAMKLGY SKPWPEAMKL ITGQPNMSAS
1210 1220 1230 1240 1250
AMMNYFKPLT EWLVTENRRH GETLGWPEYN WAPNTARAEG STAESNRVNF
1260 1270 1280 1290 1300
LGLYLEPQQA RVGQWVLLFL GVALLVATVG LAHRLYNIRN HHSLRRPHRG
1310
PQFGSEVELR HS
Length:1,312
Mass (Da):150,918
Last modified:August 30, 2005 - v3
Checksum:i7DF9F5BE91762DFF
GO
Isoform Testis-specific (identifier: P09470-2) [UniParc] [UniParc]FASTAAdd to basket
Also known as: ACE-T

The sequence of this isoform differs from the canonical sequence as follows:
     1-580: Missing.
     581-646: GCSRPWQEVL...LPDNYPEGID → MGQGWATPGL...TIDQTTQIPN

Show »
Length:732
Mass (Da):84,047
Checksum:i16C817E7FBD09BD9
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti568A → T in AAA37147 (PubMed:2545691).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0376381 – 580Missing in isoform Testis-specific. 1 PublicationAdd BLAST580
Alternative sequenceiVSP_037639581 – 646GCSRP…PEGID → MGQGWATPGLPSFLFLLLCC GHHLLVLSQVATDHVTANQG ITNQATTRSQTTTHQATIDQ TTQIPN in isoform Testis-specific. 1 PublicationAdd BLAST66

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
J04946 mRNA Translation: AAA37147.1
J04947 mRNA Translation: AAA37148.1
M55333 mRNA Translation: AAA37149.1
M61094 Genomic DNA Translation: AAA37150.1
BC040404 mRNA Translation: AAH40404.1
J03940 mRNA Translation: AAA37146.1
CCDSiCCDS25543.1 [P09470-1]
CCDS25544.1 [P09470-2]
PIRiA34171
A35655
RefSeqiNP_033728.1, NM_009598.2 [P09470-2]
NP_997507.1, NM_207624.5 [P09470-1]
UniGeneiMm.754

Genome annotation databases

EnsembliENSMUST00000001963; ENSMUSP00000001963; ENSMUSG00000020681 [P09470-1]
ENSMUST00000001964; ENSMUSP00000001964; ENSMUSG00000020681 [P09470-2]
GeneIDi11421
KEGGimmu:11421
UCSCiuc007lxu.2 mouse [P09470-1]
uc007lxw.2 mouse [P09470-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiACE_MOUSE
AccessioniPrimary (citable) accession number: P09470
Secondary accession number(s): P22967, Q6GTS2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1989
Last sequence update: August 30, 2005
Last modified: June 20, 2018
This is version 173 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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