P09466 (PAEP_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycodelin
Short name=GD

Alternative name(s):
Placental protein 14
Short name=PP14
Pregnancy-associated endometrial alpha-2 globulin
Short name=PAEG
Short name=PEG
Progestagen-associated endometrial protein
Progesterone-associated endometrial protein

Gene names

Name:

PAEP

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	180 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	This protein is, quantitatively, the main protein synthesized and secreted in the endometrium from mid-luteal phase of the menstrual cycle and during the first semester of pregnancy.
Subunit structure	Homodimer.
Subcellular location	Secreted.
Post-translational modification	At least two differentially glycosylated forms are found. Glycodelin-A (GdA) (amniotic fluid) with contraceptive and immunosuppressive activities and glycodelin-S (Gds) (seminal plasma) whose role is not yet known. The gender-specific glycosylation may serve to regulate key process involved in human reproduction. Ref.10 Ref.11 Ref.12
Sequence similarities	Belongs to the calycin superfamily. Lipocalin family.
Sequence caution	The sequence AAA60147.1 differs from that shown. Reason: Erroneous initiation. The sequence CAB43305.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Secreted
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Signal
PTM	Disulfide bond Glycoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	multicellular organismal development Traceable author statement. Source: ProtInc
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from direct assay. Source: HPA
Molecular function	binding Inferred from electronic annotation. Source: InterPro transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 3 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P09466-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P09466-2)

The sequence of this isoform differs from the canonical sequence as follows:
     33-54: Missing.

Isoform 3 (identifier: P09466-3)

The sequence of this isoform differs from the canonical sequence as follows:
     33-126: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 18	18	Ref.7 Ref.8 Ref.9
	Chain	19 – 180	162	Glycodelin		PRO_0000017953
Amino acid modifications
	Glycosylation	46	1	N-linked (GlcNAc...)		CAR_000123
	Glycosylation	81	1	N-linked (GlcNAc...)		CAR_000124
	Disulfide bond	84 ↔ 178		By similarity
	Disulfide bond	124 ↔ 137		By similarity
Natural variations
	Alternative sequence	33 – 126	94	Missing in isoform 3.		VSP_003141
	Alternative sequence	33 – 54	22	Missing in isoform 2.		VSP_003140
	Natural variant	28	1	L → V. Corresponds to variant rs34284195 [ dbSNP | Ensembl ].		VAR_050178
	Natural variant	126	1	Q → K. Corresponds to variant rs3748210 [ dbSNP | Ensembl ].		VAR_034355
Experimental info
	Sequence conflict	35 – 37	3	GTW → VTA AA sequence Ref.9
	Sequence conflict	36	1	T → K AA sequence Ref.7
	Sequence conflict	95	1	E → G Ref.1
	Sequence conflict	152	1	Q → E Ref.1

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified March 1, 1992. Version 2. Checksum: 0813A74A4231149E	FASTA	180	20,624
	10 20 30 40 50 60 MLCLLLTLGV ALVCGVPAMD IPQTKQDLEL PKLAGTWHSM AMATNNISLM ATLKAPLRVH 70 80 90 100 110 120 ITSLLPTPED NLEIVLHRWE NNSCVEKKVL GEKTENPKKF KINYTVANEA TLLDTDYDNF 130 140 150 160 170 180 LFLCLQDTTT PIQSMMCQYL ARVLVEDDEI MQGFIRAFRP LPRHLWYLLD LKQMEEPCRF
	Isoform 2 [UniParc]. Checksum: FEB77A0B313F33C8	FASTA	158	18,279
	10 20 30 40 50 60 MLCLLLTLGV ALVCGVPAMD IPQTKQDLEL PKAPLRVHIT SLLPTPEDNL EIVLHRWENN 70 80 90 100 110 120 SCVEKKVLGE KTENPKKFKI NYTVANEATL LDTDYDNFLF LCLQDTTTPI QSMMCQYLAR 130 140 150 VLVEDDEIMQ GFIRAFRPLP RHLWYLLDLK QMEEPCRF
	Isoform 3 [UniParc]. Checksum: 01C203BBE8D26652	FASTA	86	9,953
	10 20 30 40 50 60 MLCLLLTLGV ALVCGVPAMD IPQTKQDLEL PKDTTTPIQS MMCQYLARVL VEDDEIMQGF 70 80 IRAFRPLPRH LWYLLDLKQM EEPCRF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Complete amino acid sequence of human placental protein 14: a progesterone-regulated uterine protein homologous to beta-lactoglobulins." Julkunen M., Seppala M., Janne O.A. Proc. Natl. Acad. Sci. U.S.A. 85:8845-8849(1988) [PubMed: 3194393] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"Human placental protein 14 gene: sequence and characterization of a short duplication." Vaisse C., Atger M., Potier B., Milgrom E. DNA Cell Biol. 9:401-413(1990) [PubMed: 2206398] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"Multiple forms of mRNA encoding human pregnancy-associated endometrial alpha 2-globulin, a beta-lactoglobulin homologue." Garde J., Bell S.C., Eperon I.C. Proc. Natl. Acad. Sci. U.S.A. 88:2456-2460(1991) [PubMed: 2006183] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], ALTERNATIVE SPLICING.
[4]	"The full-ORF clone resource of the German cDNA consortium." Bechtel S., Rosenfelder H., Duda A., Schmidt C.P., Ernst U., Wellenreuther R., Mehrle A., Schuster C., Bahr A., Bloecker H., Heubner D., Hoerlein A., Michel G., Wedler H., Koehrer K., Ottenwaelder B., Poustka A., Wiemann S., Schupp I. BMC Genomics 8:399-399(2007) [PubMed: 17974005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Uterus.
[5]	"DNA sequence and analysis of human chromosome 9." Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L., Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R., Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S., Bagguley C.L. , Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K., Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y., Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C., Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E., Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M., Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J., Frankish A., Frankland J.A., French L., Fricker D.G., Garner P., Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S., Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E., Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D., Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E., Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K., Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J., Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E., McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V., Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S., Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K., Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J., Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M., West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L., Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M., Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J., Dunham I. Nature 429:369-374(2004) [PubMed: 15164053] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1). Tissue: Lung.
[7]	"Pregnancy-associated endometrial alpha 2-globulin, the major secretory protein of the luteal phase and first trimester pregnancy endometrium, is not glycosylated prolactin but related to beta-lactoglobulins." Bell S.C., Keyte J.W., Waites G.T. J. Clin. Endocrinol. Metab. 65:1067-1071(1987) [PubMed: 3667877] [Abstract] Cited for: PROTEIN SEQUENCE OF 19-56.
[8]	"Amino acid sequence homology between human placental protein 14 and beta-lactoglobulins from various species." Huhtala M.L., Seppala M., Narvanen A., Palomaki P., Julkunen M., Bohn H. Endocrinology 120:2620-2622(1987) [PubMed: 3569148] [Abstract] Cited for: PROTEIN SEQUENCE OF 19-30.
[9]	"Purification and characterization of an immunomodulatory endometrial protein, glycodelin." Vigne J.-L., Hornung D., Mueller M.D., Taylor R.N. J. Biol. Chem. 276:17101-17105(2001) [PubMed: 11278680] [Abstract] Cited for: PROTEIN SEQUENCE OF 19-38. Tissue: Decidua.
[10]	"Structural analysis of the oligosaccharides derived from glycodelin, a human glycoprotein with potent immunosuppressive and contraceptive activities." Dell A., Morris H.R., Easton R.L., Panico M., Patankar M., Oehniger S., Koistinen R., Koistinen H., Seppala M., Clark G.F. J. Biol. Chem. 270:24116-24126(1995) [PubMed: 7592613] [Abstract] Cited for: STRUCTURE OF CARBOHYDRATES OF GLYCODELIN-A. Tissue: Amniotic fluid.
[11]	"Gender-specific glycosylation of human glycodelin affects its contraceptive activity." Morris H.R., Dell A., Easton R.L., Panico M., Koistinen H., Koistinen R., Oehninger S., Patankar M.S., Seppala M., Clark G.F. J. Biol. Chem. 271:32159-32167(1996) [PubMed: 8943270] [Abstract] Cited for: STRUCTURE OF CARBOHYDRATES OF GLYCODELIN-S. Tissue: Seminal plasma.
[12]	"Glycodelin from seminal plasma is a differentially glycosylated form of contraceptive glycodelin-A." Koistinen H., Koistinen R., Dell A., Morris H.R., Easton R.L., Patankar M.S., Oehninger S., Clark G.F., Seppala M. Mol. Hum. Reprod. 2:759-765(1996) [PubMed: 9239694] [Abstract] Cited for: STRUCTURE OF CARBOHYDRATES.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	J04129 mRNA. Translation: AAA60147.1. Different initiation. M34046 Genomic DNA. Translation: AAA60148.1. M61886 mRNA. Translation: AAA35801.1. M61886 mRNA. Translation: AAA35802.1. AL050169 mRNA. Translation: CAB43305.1. Different initiation. AL354761 Genomic DNA. Translation: CAI17197.1. BC069451 mRNA. Translation: AAH69451.1. BC069562 mRNA. Translation: AAH69562.1. BC112304 mRNA. Translation: AAI12305.1. BC113728 mRNA. Translation: AAI13729.1.
IPI	IPI00014544. IPI00218735. IPI00218736.
PIR	A39167. A35570.
RefSeq	NP_001018059.1. NM_001018049.1. NP_002562.2. NM_002571.2.
UniGene	Hs.532325.
3D structure databases
ProteinModelPortal	P09466.
SMR	P09466. Positions 20-180.
ModBase	Search...
Protein-protein interaction databases
IntAct	P09466. 7 interactions.
MINT	MINT-1209116.
STRING	P09466.
PTM databases
GlycoSuiteDB	P09466.
Polymorphism databases
DMDM	130701.
2D gel databases
Siena-2DPAGE	P09466.
Proteomic databases
PRIDE	P09466.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000277508; ENSP00000277508; ENSG00000122133. ENST00000371766; ENSP00000360831; ENSG00000122133. ENST00000479141; ENSP00000417898; ENSG00000122133.
GeneID	5047.
KEGG	hsa:5047.
UCSC	uc004cgd.1. human.
Organism-specific databases
CTD	5047.
GeneCards	GC09P138453.
H-InvDB	HIX0008530.
HGNC	HGNC:8573. PAEP.
HPA	CAB016762. HPA020108. HPA029473.
MIM	173310. gene.
neXtProt	NX_P09466.
PharmGKB	PA32904.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG19891.
HOVERGEN	HBG104361.
InParanoid	P09466.
OMA	AMATNNI.
OrthoDB	EOG4PNXJ2.
PhylomeDB	P09466.
Gene expression databases
ArrayExpress	P09466.
Bgee	P09466.
CleanEx	HS_PAEP.
Genevestigator	P09466.
GermOnline	ENSG00000122133. Homo sapiens.
Family and domain databases
InterPro	IPR002447. Blactoglobulin. IPR012674. Calycin. IPR011038. Calycin-like. IPR002345. Lipocalin. IPR022272. Lipocalin_CS. IPR000566. Lipocln_cytosolic_FA-bd_dom. [Graphical view]
Gene3D	G3DSA:2.40.128.20. Calycin. 1 hit.
Pfam	PF00061. Lipocalin. 1 hit. [Graphical view]
PRINTS	PR01172. BLCTOGLOBULN. PR00179. LIPOCALIN.
SUPFAM	SSF50814. Calycin. 1 hit.
PROSITE	PS00213. LIPOCALIN. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	19446.
SOURCE	Search...

Entry information

Entry name

PAEP_HUMAN

Accession

Primary (citable) accession number: P09466
Secondary accession number(s): Q5T6T1, Q9UG92

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1989
Last sequence update:	March 1, 1992
Last modified:	January 25, 2012
This is version 118 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.